scholarly journals Gelatin/Chitosan Bilayer Patches Loaded with Cortex Phellodendron amurense/Centella asiatica Extracts for Anti-Acne Application

Polymers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 579
Author(s):  
Chi-Wen Kuo ◽  
Yi-Fang Chiu ◽  
Min-Hua Wu ◽  
Ming-Hsien Li ◽  
Cheng-Nan Wu ◽  
...  
Keyword(s):  
Water Retention ◽  
Lower Layer ◽  
Retention Rate ◽  
Centella Asiatica ◽  
Fibroblast Cell ◽  
In Vitro Cytotoxicity ◽  
Hypoxic Conditions ◽  
Different Temperatures ◽  
Outer Water

Acne is a chronic inflammatory skin disease that often occurs with anaerobic Propionibacterium acnes (P. acnes). Anti-acne patches, made of hydrocolloid or hydrogel, have become a popular way of topical treatment. The outer water-impermeable layer of commercial patches might create hypoxic conditions and promote P. acnes growth. In this study, gelatin/chitosan (GC) bilayer patches were prepared at different temperatures that included room temperature (RT), −20 °C/RT, and −80 °C/RT. The most promising GC bilayer patch (−80 °C /RT) contained a dense upper layer for protection from bacteria and infection and a porous lower layer for absorbing pus and fluids from pimples. The anti-acne bilayer patch was loaded with Cortex Phellodendri amurensis (PA) and Centella asiatica (CA) extracts. PA extract could inhibit the growth of P. acnes and CA extract was reported to improve wound healing and reduce scar formation. Moreover, the water retention rate, weight loss rate, antibacterial activity, and in vitro cytotoxicity of the patches were investigated. The porous structure of the patches promoted water retention and contributed to absorbing the exudate when used on open acne wounds. The GC bilayer patches loaded with PA/CA extracts were demonstrated to inhibit the growth of P. acnes, and accelerate the skin fibroblast cell viability. Based on their activities and characteristics, the GC bilayer patches with PA/CA extract prepared at −80 °C/RT obtain the potential for the application of acne spot treatment.

PREPARATION AND EVALUATION OF GAG-INCORPORATED SKIN SUBSTITUTE: AN IN VITRO STUDY

2006 ◽  
Vol 18 (03) ◽  
pp. 153-157 ◽  
Author(s):  
TZU-WEI WANG ◽  
HSI-CHIN WU ◽  
YI-CHAU HUANG ◽  
JUI-SHENG SUN ◽  
FENG-HUEI LIN
Keyword(s):  
Lower Layer ◽  
In Vitro Study ◽  
Dermal Fibroblasts ◽  
Skin Equivalent ◽  
Skin Substitute ◽  
Epidermal Keratinocytes ◽  
Different Temperatures ◽  
Air Liquid Interface ◽  
Epidermis Structure

A bi-layered gelatin-C6S-HA membrane with different pore sizes was prepared by freeze-drying at different temperatures - 20°C and -196°C, respectively Glycosaminoglycans (GAGs) were incorporated within the gelatin matrices to mimic the dermal composition and to create an appropriate environment for cell growth. The gelatin-C6S-HA membrane was cross-linked by 1-ethyl-3(3-dimethylaminopropryl) carbodiimide (EDC) to resist rapidly biodegradation by matrix enzymes. In this study, the lower layer of the sponge was inoculated with dermal fibroblasts for dermis development and as the feeder layer for epidermal keratinocytes. The upper layer was seeded with keratinocytes for epidermalization. After cultured for a period of time in air-liquid interface, the upper layer was developed into an epidermis structure with stratified epidermal layers. The lower part was developed into dermis-like structure synthesized by dermal fibroblasts surrounding with its own secreted extracellular matrix. In brief, the bi-layered skin equivalent with biological dermal analog and epidermal analog would be a suitable tool for autologous skin equivalent tissue engineering.

scholarly journals Development of Bioinspired Gelatin and Gelatin/Chitosan Bilayer Hydrofilms for Wound Healing

Pharmaceutics ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 314 ◽  
Author(s):  
Itxaso Garcia-Orue ◽  
Edorta Santos-Vizcaino ◽  
Alaitz Etxabide ◽  
Jone Uranga ◽  
Ardeshir Bayat ◽  
...  
Keyword(s):  
Wound Healing ◽  
Citric Acid ◽  
Wound Dressing ◽  
Ex Vivo ◽  
Lower Layer ◽  
Transmission Rate ◽  
Dry Weight ◽  
Porous Matrix ◽  
In Vitro Cytotoxicity

In the current study, we developed a novel gelatin-based bilayer wound dressing. We used different crosslinking agents to confer unique properties to each layer, obtaining a bioinspired multifunctional hydrofilm suitable for wound healing. First, we produced a resistant and non-degradable upper layer by lactose-mediated crosslinking of gelatin, which provided mechanical support and protection to overall design. For the lower layer, we crosslinked gelatin with citric acid, resulting in a porous matrix with a great swelling ability. In addition, we incorporated chitosan into the lower layer to harness its wound healing ability. FTIR and SEM analyses showed that lactose addition changed the secondary structure of gelatin, leading to a more compact and smoother structure than that obtained with citric acid. The hydrofilm was able to swell 384.2 ± 57.2% of its dry weight while maintaining mechanical integrity. Besides, its water vapour transmission rate was in the range of commercial dressings (1381.5 ± 108.6 g/m2·day). In vitro, cytotoxicity assays revealed excellent biocompatibility. Finally, the hydrofilm was analysed through an ex vivo wound healing assay in human skin. It achieved similar results to the control in terms of biocompatibility and wound healing, showing suitable characteristics to be used as a wound dressing.

scholarly journals Synthesis and biological activity evaluation of new functionally substituted 5-arylidene-2,4-dioxotetrahydro-1,3-thiazoles

2006 ◽  
Vol 71 (8-9) ◽  
pp. 861-866 ◽  
Author(s):  
Katarina Popov-Pergal ◽  
Milica Rancic ◽  
Miroslav Pergal ◽  
Gordana Bogdanovic ◽  
Vesna Kojic ◽  
...  
Keyword(s):  
Human Tumor ◽  
Fibroblast Cell ◽  
In Vitro Cytotoxicity ◽  
Normal Lung ◽  
Cholesteryl Esters ◽  
Tumor Cell Lines ◽  
Human Tumor Cell ◽  
Human Tumor Cell Lines ◽  
Lung Fibroblast Cell Line

New functionally substituted 5-arylidene-2,4-dioxotetrahydro 1,3-thiazole- 3-carboxylic acid cholesteryl esters were synthesized from 2,4-dioxotetrahydro- 1,3-thiazole and evaluated for their in vitro cytotoxicity against several human tumor cell lines and one normal lung fibroblast cell line.

scholarly journals Assessment of environmental condition and drying process of the plants on the concentration of alkaloids and cytotoxicity of traditional Ayahuasca Tea

2021 ◽  
Vol 10 (2) ◽  
pp. 075-089
Author(s):  
Miranda Ordilena Ferreira de ◽  
Almeida Marcílio de ◽  
Sousa Ilza Maria de Oliveira ◽  
Ruiz Ana Lúcia Tasca Gois ◽  
Costa José Luiz da ◽  
...  
Keyword(s):  
High Performance ◽  
Colorimetric Assay ◽  
Human Keratinocytes ◽  
In Vitro Cytotoxicity ◽  
Drying Method ◽  
Drying Process ◽  
Forced Circulation ◽  
Different Temperatures ◽  
Array Detection

Introduction: Ayahuasca is a traditional psychoactive tea of Amazonian indigenous, used medicinal and spiritual purposes. Wide variation in the concentration of N,N-dimethyltryptamine (DMT), Harmaline (HRL), Harmine (HRM) and Tetrahydroharmine (THH) alkaloids in Ayahuasca has been reported worldwide. Objective: To evaluate the causes of variations in alkaloids concentrations of Ayahuasca prepared with fresh and dehydrated plants from different environments and determine the best drying method to plants according to alkaloids content and cytotoxicity of Ayahuasca tea. Material and methods: The environment interference on the alkaloids of the two species was evaluated in samples of Ayahuasca tea prepared with fresh plants. The most suitable drying process to the two species was evaluated in sample Ayahuasca tea prepared with plants submitted to drying under the sun conditions and five different temperatures in forced circulation oven. The concentration of the alkaloids determined by high performance liquid chromatography with UV-vis detector with diode array detection (HPLC-DAD). The in vitro cytotoxicity of Ayahuasca was evaluated in human keratinocytes cells (HaCaT) by colorimetric assay. Results: Environmental characteristics, preparation process and temperature of plants drying interfered on DMT, HRL, HRM and THH concentrations of Ayahuasca. No effect cytotoxicity was detected with relationship to psychoactive alkaloids in samples of Ayahuasca tea prepared with fresh or dried plants. Conclusion: Concentration of DMT, HRL, HRM and THH alkaloids in Ayahuasca are influenced by plants environmental. The most suitable drying process was obtained in forced circulation oven at 43 and 45°C to P. viridis leaves and B. caapi stems respectively. The Ayahuasca prepared with fresh or dry plants no showed cytotoxicity in human keratinocytes cells.

Synthesis, Structure, and Selective Cytotoxicity of Organometallic Cp*RuII O-Alkyl-N-phenylcarbamate Sandwich Complexes

2010 ◽  
Vol 63 (2) ◽  
pp. 245 ◽  
Author(s):  
Bradley T. Loughrey ◽  
Michael L. Williams ◽  
Thomas J. Carruthers ◽  
Peter G. Parsons ◽  
Peter C. Healy
Keyword(s):  
Fibroblast Cell ◽  
In Vitro Cytotoxicity ◽  
Sandwich Complexes ◽  
One Pot ◽  
Cytotoxicity Studies ◽  
Structure Determinations ◽  
Normal Human ◽  
Fourier Transform Ir ◽  
Potent Growth

Tetraphenylborate salts of the η6-arene Cp*RuII O-alkyl-N-phenyl carbamate organometallic sandwich complexes, [Cp*Ru(PhNHCO2R)]BPh4 for R = Me (1), Et (2), and n-Pr (3), have been prepared by a facile one-pot reaction between ruthenium trichloride, pentamethylcyclopentadiene, and phenylisocyanate in refluxing alcohol solutions, and have been characterized by Fourier-transform IR and NMR spectroscopy, electrospray mass spectrometry, and single-crystal X-ray structure determinations. In vitro cytotoxicity studies show the complexes to be potent growth inhibitors for a range of tumour cell lines, while expressing significantly lower levels of toxicity towards a normal human fibroblast cell line.

Bone-like growth of hydroxyapatite in the biomimetic coating of Ti-6Al-4V alloy pretreated with protein at 25 °C

2009 ◽  
Vol 24 (6) ◽  
pp. 2145-2153 ◽  
Author(s):  
Jui Chakraborty ◽  
Matjaz Mazaj ◽  
Renu Kapoor ◽  
S. Pavana Gouri ◽  
Nina Daneu ◽  
...  
Keyword(s):  
Substrate Surface ◽  
Fibroblast Cell ◽  
Biological Properties ◽  
Physical Structure ◽  
In Vitro Cytotoxicity ◽  
X Ray ◽  
Biomimetic Coating ◽  
Periodic Replacement ◽  
Nih 3T3

Commercial-grade dense Ti-6Al-4V alloy substrate was mechanically roughened, cleaned, and treated with a globular protein [bovine serum albumin (BSA)] for 4 h. Biomimetic calcium phosphate (Ca-P) coating was applied onto the above-treated substrate by immersion into simulated body fluid (SBF) at 25 °C for a period of 4 days, with periodic replacement by freshly prepared SBF at 48-h intervals. After 4 days, branched micron-sized fibers of hydroxyapatite (HAp), resembling the structure of bone, were obtained, connecting the clusters of HAp crystal plates in the coating (thickness ∼200 μm) on the substrate surface. Structural and compositional characterization of the coating was carried out using field emission scanning electron microscopy (FE-SEM) with energy-dispersive x-ray analysis unit (EDX) facility, x-ray diffraction (XRD), and Fourier transform infrared (FTIR) data. In vitro cytotoxicity (ISO 10993-5, 1999), cell adhesion assays, and phase contrast microscopy were performed using NIH 3T3 fibroblast cell lines to ascertain the bioactivity of the coated substrates, with and without protein treatment. Based on our study, we propose a correlation between a specific physical structure of the HAp coating and its biological properties.

scholarly journals Cell behavior on silica-hydroxyapatite coaxial composite

PLoS ONE ◽  
2021 ◽  
Vol 16 (5) ◽  
pp. e0246256
Author(s):  
Jesús Alberto Garibay-Alvarado ◽  
Ericka Berenice Herrera-Ríos ◽  
Claudia Lucía Vargas-Requena ◽  
Álvaro de Jesús Ruíz-Baltazar ◽  
Simón Yobanny Reyes-López
Keyword(s):  
Tissue Regeneration ◽  
Water Retention ◽  
Structural Characteristics ◽  
Sol Gel ◽  
Average Diameter ◽  
Fibroblast Cell ◽  
Cell Behavior ◽  
Polymeric Fibers ◽  
20 Nm

Progress in the manufacture of scaffolds in tissue engineering lies in the successful combination of materials such as bioceramics having properties as porosity, biocompatibility, water retention, protein adsorption, mechanical strength and biomineralization. Hydroxyapatite (HA) is a ceramic material with lots of potential in tissue regeneration, however, its structural characteristics need to be improved for better performance. In this study, silica-hydroxyapatite (SiO2-HA) non-woven ceramic electrospunned membranes were prepared through the sol-gel method. Infrared spectra, scanning electron microscopy and XRD confirmed the structure and composition of composite. The obtained SiO2-HA polymeric fibers had approximately 230±20 nm in diameter and were then sintered at 800°C average diameter decreased to 110±17 nm. Three configurations of the membranes were obtained and tested in vitro, showing that the composite of SiO2-HA fibers showed a high percentage of viability on a fibroblast cell line. It is concluded that the fibers of SiO2-HA set in a coaxial configuration may be helpful to develop materials for bone regeneration.

scholarly journals Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes

2021 ◽  
Vol 2021 ◽  
pp. 1-18
Author(s):  
Edina H. Avdović ◽  
Isidora P. Petrović ◽  
Milena J. Stevanović ◽  
Luciano Saso ◽  
Jasmina M. Dimitrić Marković ◽  
...  
Keyword(s):  
Biological Activities ◽  
Cation Radical ◽  
Fibroblast Cell ◽  
Docking Studies ◽  
In Vitro Cytotoxicity ◽  
Toxicity Tests ◽  
Cytotoxic Activities ◽  
Spectroscopic Techniques

Two newly synthesized 4-hydroxycoumarin bidentate ligands (L1 and L2) and their palladium(II) complexes (C1 and C2) were screened for their biological activities, in vitro and in vivo. Structures of new compounds were established based on elemental analysis, 1H NMR, 13C NMR, and IR spectroscopic techniques. The obtained compounds were tested for their antioxidative and cytotoxic activities and results pointed to selective antiradical activity of palladium(II) complexes towards •OH and -•OOH radicals and anti-ABTS (2,2 ′ -Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical) activity comparable to that of ascorbate. Results indicated the effect of C1 and C2 on the enzymatic activity of the antioxidative defense system. In vitro cytotoxicity assay performed on different carcinoma cell lines (HCT166, A375, and MIA PaCa-2), and one healthy fibroblast cell line (MRC-5) showed a cytotoxic effect of both C1 and C2, expressed as a decrease in carcinoma cells’ viability, mostly by induction of apoptosis. In vivo toxicity tests performed on zebrafish embryos indicated different effects of C1 and C2, ranging from adverse developmental effect to no toxicity, depending on tested concentration. According to docking studies, both complexes (C1 and C2) showed better inhibitory activity in comparison to other palladium(II) complexes.

In vitro cytotoxicity evaluation of plastic biomedical devices

2001 ◽  
Vol 20 (8) ◽  
pp. 412-417 ◽  
Author(s):  
A B Pant ◽  
A K Agarwal ◽  
V P Sharma ◽  
P K Seth
Keyword(s):  
Growth Inhibition ◽  
Fibroblast Cell ◽  
Essential Amino Acids ◽  
In Vitro Cytotoxicity ◽  
Mouse Fibroblast ◽  
Biomedical Devices ◽  
Minimum Essential Medium ◽  
Growth Inhibition Assay ◽  
Mouse Fibroblast Cell Line

Cytotoxic potential of four plastic biomedical devices (intravenous transfusion sets, IV sets; dextrose normal saline bottles, DNS bottles; Ringer lactate bottles, RL bottles; and Ryle's tubes) including 17different brands was evaluated by investigating growth inhibition, percent survival, mitotic index and colony-forming ability (cfa) in L929, an adherent type mouse fibroblast cell line. Experimental sets were exposed with leachates of biomedical products in serumfree minimum essential medium (MEM) for 1 h at 378Cina CO2 incubator. After 1 h, medium was replaced with serumrich MEM containing essential amino acids and reincubated up to 96 h. Cells in serum-free MEM only were processed under identical conditions and served as the control. The leachates from all types of biomedical devices evaluated exhibitedreductioninthegrowthandsurvivalofthecellline in the first 12 h postexposure followed by their gradual recoveryupto96h.Asignificantreductionincellgrowthwas apparent in the six brands of IV sets from 24 h onwards up to 36 h (59% growth inhibition). Though the cfa was also reduced in all the brands tested, the magnitude of reduction was less compared to growth inhibition. The results indicate that leachates of IV sets were more toxic compared to other biomedical devices screened, and growth inhibition assay was found to be more sensitive and suitable for cytotoxicity evaluation of biomedical devices.

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