scholarly journals Hybrid Collagen Hydrogel/Chondroitin-4-Sulphate Fortified with Dermal Fibroblast Conditioned Medium for Skin Therapeutic Application

Polymers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 508
Author(s):  
Manira Maarof ◽  
Masrina Mohd Nadzir ◽  
Lau Sin Mun ◽  
Mh Busra Fauzi ◽  
Shiplu Roy Chowdhury ◽  
...  
Keyword(s):  
Conditioned Medium ◽  
Dermal Fibroblast ◽  
Lower Percentage ◽  
Skin Substitute ◽  
Infrared Spectrometry ◽  
3 Dimensional ◽  
Specific Medium ◽  
Collagen Hydrogel ◽  
3D Collagen ◽  
Semi Solid

The current strategy for rapid wound healing treatment involves combining a biomaterial and cell-secreted proteins or biomolecules. This study was aimed at characterizing 3-dimensional (3D) collagen hydrogels fortified with dermal fibroblast-conditioned medium (DFCM) as a readily available acellular skin substitute. Confluent fibroblasts were cultured with serum-free keratinocyte-specific medium (KM1 and KM2) and fibroblast-specific medium (FM) to obtain DFCM. Subsequently, the DFCM was mixed with collagen (Col) hydrogel and chondroitin-4-sulphate (C4S) to fabricate 3D constructs termed Col/C4S/DFCM-KM1, Col/C4S/DFCM-KM2, and Col/C4S/DFCM-FM. The constructs successfully formed soft, semi-solid and translucent hydrogels within 1 h of incubation at 37 °C with strength of <2.5 Newton (N). The Col/C4S/DFCM demonstrated significantly lower turbidity compared to the control groups. The Col/C4S/DFCM also showed a lower percentage of porosity (KM1: 35.15 ± 9.76%; KM2: 6.85 ± 1.60%; FM: 14.14 ± 7.65%) compared to the Col (105.14 ± 11.87%) and Col/C4S (143.44 ± 27.72%) constructs. There were no changes in both swelling and degradation among all constructs. Fourier transform infrared spectrometry showed that all groups consisted of oxygen–hydrogen bonds (O-H) and amide I, II, and III. In conclusion, the Col/C4S/DFCM constructs maintain the characteristics of native collagen and can synergistically deliver essential biomolecules for future use in skin therapeutic applications.

scholarly journals Concentration Dependent Effect of Human Dermal Fibroblast Conditioned Medium (DFCM) from Three Various Origins on Keratinocytes Wound Healing

2020 ◽  
Vol 21 (8) ◽  
pp. 2929
Author(s):  
Manira Maarof ◽  
Shiplu Roy Chowdhury ◽  
Aminuddin Saim ◽  
Ruszymah Bt Hj Idrus ◽  
Yogeswaran Lokanathan
Keyword(s):  
Wound Healing ◽  
Conditioned Medium ◽  
Dermal Fibroblast ◽  
Human Dermal Fibroblast ◽  
Control Group ◽  
Serum Free ◽  
Specific Medium ◽  
Polymerase Chain ◽  
And Migration

Fibroblasts secrete many essential factors that can be collected from fibroblast culture medium, which is termed dermal fibroblast conditioned medium (DFCM). Fibroblasts isolated from human skin samples were cultured in vitro using the serum-free keratinocyte-specific medium (Epilife (KM1), or define keratinocytes serum-free medium, DKSFM (KM2) and serum-free fibroblast-specific medium (FM) to collect DFCM-KM1, DFCM-KM2, and DFCM-FM, respectively). We characterised and evaluated the effects of 100–1600 µg/mL DFCM on keratinocytes based on attachment, proliferation, migration and gene expression. Supplementation with 200–400 µg/mL keratinocyte-specific DFCM-KM1 and DFCM-KM2 enhanced the attachment, proliferation and migration of sub-confluent keratinocytes, whereas 200–1600 µg/mL DFCM-FM significantly increased the healing rate in the wound healing assay, and 400–800 µg/mL DFCM-FM was suitable to enhance keratinocyte attachment and proliferation. A real-time (RT2) profiler polymerase chain reaction (PCR) array showed that 42 genes in the DFCM groups had similar fold regulation compared to the control group and most of the genes were directly involved in wound healing. In conclusion, in vitro keratinocyte re-epithelialisation is supported by the fibroblast-secreted proteins in 200–400 µg/mL DFCM-KM1 and DFCM-KM2, and 400–800 µg/mL DFCM-FM, which could be useful for treating skin injuries.

Safety and efficacy of dermal fibroblast conditioned medium (DFCM) fortified collagen hydrogel as acellular 3D skin patch

2018 ◽  
Vol 9 (1) ◽  
pp. 144-161 ◽  
Author(s):  
Manira Maarof ◽  
Mohd Fauzi Mh Busra ◽  
Yogeswaran Lokanathan ◽  
Ruszymah Bt Hj Idrus ◽  
Nor Fadilah Rajab ◽  
...  
Keyword(s):  
Conditioned Medium ◽  
Dermal Fibroblast ◽  
Safety And Efficacy ◽  
Collagen Hydrogel ◽  
Skin Patch ◽  
3D Skin

Efficacy of dental pulp-derived stem cells conditioned medium loaded in collagen hydrogel in spinal cord injury in rats: Stereological evidence

2021 ◽  
Vol 116 ◽  
pp. 101978
Author(s):  
Reza Asadi-Golshan ◽  
Vahid Razban ◽  
Esmaeil Mirzaei ◽  
Abdolkarim Rahmanian ◽  
Sahar Khajeh ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Conditioned Medium ◽  
Dental Pulp ◽  
Collagen Hydrogel ◽  
Cord Injury

scholarly journals Bone Morphogenetic Protein 4 (BMP4) Enhances the Differentiation of Human Induced Pluripotent Stem Cells into Limbal Progenitor Cells

2021 ◽  
Vol 43 (3) ◽  
pp. 2124-2134
Author(s):  
Hyun Soo Lee ◽  
Jeewon Mok ◽  
Choun-Ki Joo
Keyword(s):  
Progenitor Cells ◽  
Dermal Fibroblast ◽  
Ips Cells ◽  
Human Dermal Fibroblast ◽  
Corneal Epithelial ◽  
Specific Medium ◽  
Morphogenetic Protein ◽  
Human Ips Cells ◽  
Limbal Stem Cell ◽  
Induced Pluripotent

Corneal epithelium maintains visual acuity and is regenerated by the proliferation and differentiation of limbal progenitor cells. Transplantation of human limbal progenitor cells could restore the integrity and functionality of the corneal surface in patients with limbal stem cell deficiency. However, multiple protocols are employed to differentiate human induced pluripotent stem (iPS) cells into corneal epithelium or limbal progenitor cells. The aim of this study was to optimize a protocol that uses bone morphogenetic protein 4 (BMP4) and limbal cell-specific medium. Human dermal fibroblast-derived iPS cells were differentiated into limbal progenitor cells using limbal cell-specific (PI) medium and varying doses (1, 10, and 50 ng/mL) and durations (1, 3, and 10 days) of BMP4 treatment. Differentiated human iPS cells were analyzed by real-time polymerase chain reaction (RT-PCR), Western blotting, and immunocytochemical studies at 2 or 4 weeks after BMP4 treatment. Culturing human dermal fibroblast-derived iPS cells in limbal cell-specific medium and BMP4 gave rise to limbal progenitor and corneal epithelial-like cells. The optimal protocol of 10 ng/mL and three days of BMP4 treatment elicited significantly higher limbal progenitor marker (ABCG2, ∆Np63α) expression and less corneal epithelial cell marker (CK3, CK12) expression than the other combinations of BMP4 dose and duration. In conclusion, this study identified a successful reprogramming strategy to induce limbal progenitor cells from human iPS cells using limbal cell-specific medium and BMP4. Additionally, our experiments indicate that the optimal BMP4 dose and duration favor limbal progenitor cell differentiation over corneal epithelial cells and maintain the phenotype of limbal stem cells. These findings contribute to the development of therapies for limbal stem cell deficiency disorders.

scholarly journals In vitro callogenesis induction of Guarianthe skinneri (Bateman) Dressler & W.E. Higgins (Orchidaceae)

2017 ◽  
Vol 66 (2) ◽  
Author(s):  
Ana Gabriela Coutiño-Cortés ◽  
Vincenzo Bertolini ◽  
Leobardo Iracheta Donjuan ◽  
Lorena Ruíz-Montoya ◽  
Javier Francisco Valle-Mora
Keyword(s):  
Statistical Analysis ◽  
Solid Medium ◽  
Callus Formation ◽  
Natural Habitat ◽  
Leaf Explants ◽  
Lower Percentage ◽  
Production Technique ◽  
Flowering Season ◽  
Semi Solid

Guarianthe skinneri (Bateman) Dressler & W.E. Higgins., is a native orchid from Mexico, considered as threatened species by NOM-ECOL-059-SEMARNAT-2010, mainly due to the disappearance of its natural habitat and the illegal collection during its flowering season. The aim of this research was to induce in vitro callogenesis from different type of explants, using phytoregulators, in order to look for a massive production technique to contribute to its conservation. We evaluated the leaf and pseudobulb marrow explants growing in semi-solid medium MS adding BAP, 2, 4-D, Kin, the interaction of BAP/2, 4-D/Kin/Sad and a control without any type of plant growth regulators. Statistical analysis showed the pseudobulb marrow explants are more suitable for in vitro introduction in comparison to leaf explants, since they perform a lower percentage of contamination (18.8% in marrow and 73.2% in leaves). Likewise, the pseudobulb marrow explants increased callus formation (10.8%) in comparison to leaf explants (7.6%). Regarding the phytoregulators employed, BAP have allowed to increased callus formation (17%) compared to other phytoregulators (7-10%). This is the first report, which proposes the use of pseudobulb marrow as explant for callus induction in G. skinneri.

scholarly journals Improving motor neuron-like cell differentiation of hEnSCs by the combination of epothilone B loaded PCL microspheres in optimized 3D collagen hydrogel

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Narges Mahmoodi ◽  
Jafar Ai ◽  
Zahra Hassannejad ◽  
Somayeh Ebrahimi-Barough ◽  
Elham Hasanzadeh ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Cell Differentiation ◽  
Motor Neuron ◽  
Three Dimensional ◽  
Spinal Cord Regeneration ◽  
Epothilone B ◽  
Collagen Hydrogel ◽  
Cord Injury ◽  
3D Collagen ◽  
3D Composite

AbstractSpinal cord regeneration is limited due to various obstacles and complex pathophysiological events after injury. Combination therapy is one approach that recently garnered attention for spinal cord injury (SCI) recovery. A composite of three-dimensional (3D) collagen hydrogel containing epothilone B (EpoB)-loaded polycaprolactone (PCL) microspheres (2.5 ng/mg, 10 ng/mg, and 40 ng/mg EpoB/PCL) were fabricated and optimized to improve motor neuron (MN) differentiation efficacy of human endometrial stem cells (hEnSCs). The microspheres were characterized using liquid chromatography-mass/mass spectrometry (LC-mas/mas) to assess the drug release and scanning electron microscope (SEM) for morphological assessment. hEnSCs were isolated, then characterized by flow cytometry, and seeded on the optimized 3D composite. Based on cell morphology and proliferation, cross-linked collagen hydrogels with and without 2.5 ng/mg EpoB loaded PCL microspheres were selected as the optimized formulations to compare the effect of EpoB release on MN differentiation. After differentiation, the expression of MN markers was estimated by real-time PCR and immunofluorescence (IF). The collagen hydrogel containing the EpoB group had the highest HB9 and ISL-1 expression and the longest neurite elongation. Providing a 3D permissive environment with EpoB, significantly improves MN-like cell differentiation and maturation of hEnSCs and is a promising approach to replace lost neurons after SCI.

Proteomic Analysis of Human Dermal Fibroblast Conditioned Medium (DFCM)

2018 ◽  
Vol 37 (6) ◽  
pp. 589-607 ◽  
Author(s):  
Manira Maarof ◽  
Yogeswaran Lokanathan ◽  
Hj Idrus Ruszymah ◽  
Aminuddin Saim ◽  
Shiplu Roy Chowdhury
Keyword(s):  
Conditioned Medium ◽  
Proteomic Analysis ◽  
Dermal Fibroblast ◽  
Human Dermal Fibroblast

PDGF-D promotes dermal fibroblast invasion in 3-dimensional extracellular matrix via Snail-mediated MT1-MMP upregulation

Tumor Biology ◽  
2015 ◽  
Vol 37 (1) ◽  
pp. 591-599 ◽  
Author(s):  
Zhuo Qin ◽  
Jinfa Feng ◽  
Yusi Liu ◽  
Li-Li Deng ◽  
Changlian Lu
Keyword(s):  
Extracellular Matrix ◽  
Dermal Fibroblast ◽  
3 Dimensional

Electrophysiological characterization of embryonic hippocampal neurons cultured in a 3D collagen hydrogel

Biomaterials ◽  
2009 ◽  
Vol 30 (26) ◽  
pp. 4377-4383 ◽  
Author(s):  
Tao Xu ◽  
Peter Molnar ◽  
Cassie Gregory ◽  
Mainak Das ◽  
Thomas Boland ◽  
...  
Keyword(s):  
Hippocampal Neurons ◽  
Collagen Hydrogel ◽  
3D Collagen ◽  
Electrophysiological Characterization

scholarly journals Composition, properties, stability and thermal behavior of tamarind (Tamarindus indica) seed oil

2019 ◽  
Vol 70 (4) ◽  
pp. 333 ◽  
Author(s):  
M. G. Chacón-Fernández ◽  
M. R. Hernández-Medel ◽  
M. Bernal-González ◽  
M. C. Durán-Domínguez-de-Bazúa ◽  
J. A. Solís-Fuentes
Keyword(s):  
Fatty Acids ◽  
Phase Behavior ◽  
Seed Oil ◽  
Inert Atmosphere ◽  
Gas Chromatography Mass Spectrometry ◽  
Infrared Spectrometry ◽  
Tamarindus Indica ◽  
Air Atmosphere ◽  
Tamarind Seed ◽  
Semi Solid

The composition, thermal stability and phase behavior of tamarind (Tamarindus indica) seed oil were analyzed to contribute to the exploration of their potential uses. The oil was extracted from the kernel of the tamarind seed with hexane, and its main physical, chemical and thermal properties were analyzed by infrared spectrometry, gas chromatography-mass spectrometry, DSC, and TGA. The results showed that the tamarind seed had a 3.76 ± 0.20% oil with a saponification index of 174.80 ± 9.87 mg KOH/g and the major fatty acids were lignoceric (20.15%), oleic (18.99%) and palmitic (11.99%). Stearic, behenic, linoleic, arachidic, and other fatty acids were also present. TGA and DSC showed that in an inert atmosphere, the triacylglycerols of tamarind seed oil (TSO) are decomposed in a single stage that starts at 224.1 °C and in an air atmosphere in three stages, initiating its decomposition at 218 °C. The TSO showed crystallization and fusion curves with a single maximum peak with Tonset and Toffset of 20.16 and ?38.8 °C and ?22.2 and 28.6 °C, respectively. The solid fat profile of the oil showed a semi-solid and liquid consistency in the ambient temperature range. The composition, thermal and phase behavior showed that TSO is potentially useful for alimentary, pharmacological, and cosmetological purposes.

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