scholarly journals Viscosity-Regulated Control of RNA Microstructure Fabrication

Polymers ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 454
Author(s):  
Sunghyun Moon ◽  
Hyejin Kim ◽  
Dajeong Kim ◽  
Jong Bum Lee
Keyword(s):  
Self Assembly ◽  
Biomedical Applications ◽  
Polymerization Rate ◽  
Solution Viscosity ◽  
Assembly Process ◽  
New Approach ◽  
Precise Control ◽  
Microstructure Fabrication ◽  
Wide Range ◽  
Rna Polymerization

The development of RNA self-assemblies offers a powerful platform for a wide range of biomedical applications. The fabrication process has become more elaborate in order to achieve functional structures with maximized potential. As a facile means to control the structure, here, we report a new approach to manipulate the polymerization rate and subsequent self-assembly process through regulation of the reaction viscosity. As the RNA polymerization rate has a dependence on solution viscosity, the resulting assembly, crystallization, and overall sizes of the product could be manipulated. The simple and precise control of RNA polymerization and self-assembly by reaction viscosity will provide a way to widen the utility of RNA-based materials.

Factors Affecting Molecular Self-Assembly and Its Mechanism

2012 ◽  
Vol 9 (1) ◽  
pp. 43 ◽  
Author(s):  
Hueyling Tan
Keyword(s):  
Self Assembly ◽  
Building Blocks ◽  
Molecular Engineering ◽  
Molecular Structures ◽  
Polymer Science ◽  
New Approach ◽  
Factors Affecting ◽  
Dna Structures ◽  
Self Assembling ◽  
Wide Range

Molecular self-assembly is ubiquitous in nature and has emerged as a new approach to produce new materials in chemistry, engineering, nanotechnology, polymer science and materials. Molecular self-assembly has been attracting increasing interest from the scientific community in recent years due to its importance in understanding biology and a variety of diseases at the molecular level. In the last few years, considerable advances have been made in the use ofpeptides as building blocks to produce biological materials for wide range of applications, including fabricating novel supra-molecular structures and scaffolding for tissue repair. The study ofbiological self-assembly systems represents a significant advancement in molecular engineering and is a rapidly growing scientific and engineering field that crosses the boundaries ofexisting disciplines. Many self-assembling systems are rangefrom bi- andtri-block copolymers to DNA structures as well as simple and complex proteins andpeptides. The ultimate goal is to harness molecular self-assembly such that design andcontrol ofbottom-up processes is achieved thereby enabling exploitation of structures developed at the meso- and macro-scopic scale for the purposes oflife and non-life science applications. Such aspirations can be achievedthrough understanding thefundamental principles behind the selforganisation and self-synthesis processes exhibited by biological systems.

scholarly journals Modification of a Single Atom Affects the Physical Properties of Double Fluorinated Fmoc-Phe Derivatives

2021 ◽  
Vol 22 (17) ◽  
pp. 9634
Author(s):  
Moran Aviv ◽  
Dana Cohen-Gerassi ◽  
Asuka A. Orr ◽  
Rajkumar Misra ◽  
Zohar A. Arnon ◽  
...  
Keyword(s):  
Amino Acid ◽  
Physical Properties ◽  
Self Assembly ◽  
Deep Understanding ◽  
Building Blocks ◽  
The Self ◽  
Single Atom ◽  
Supramolecular Organization ◽  
Assembly Process ◽  
Wide Range

Supramolecular hydrogels formed by the self-assembly of amino-acid based gelators are receiving increasing attention from the fields of biomedicine and material science. Self-assembled systems exhibit well-ordered functional architectures and unique physicochemical properties. However, the control over the kinetics and mechanical properties of the end-products remains puzzling. A minimal alteration of the chemical environment could cause a significant impact. In this context, we report the effects of modifying the position of a single atom on the properties and kinetics of the self-assembly process. A combination of experimental and computational methods, used to investigate double-fluorinated Fmoc-Phe derivatives, Fmoc-3,4F-Phe and Fmoc-3,5F-Phe, reveals the unique effects of modifying the position of a single fluorine on the self-assembly process, and the physical properties of the product. The presence of significant physical and morphological differences between the two derivatives was verified by molecular-dynamics simulations. Analysis of the spontaneous phase-transition of both building blocks, as well as crystal X-ray diffraction to determine the molecular structure of Fmoc-3,4F-Phe, are in good agreement with known changes in the Phe fluorination pattern and highlight the effect of a single atom position on the self-assembly process. These findings prove that fluorination is an effective strategy to influence supramolecular organization on the nanoscale. Moreover, we believe that a deep understanding of the self-assembly process may provide fundamental insights that will facilitate the development of optimal amino-acid-based low-molecular-weight hydrogelators for a wide range of applications.

scholarly journals Aspiration-assisted bioprinting for precise positioning of biologics

2020 ◽  
Vol 6 (10) ◽  
pp. eaaw5111 ◽  
Author(s):  
Bugra Ayan ◽  
Dong Nyoung Heo ◽  
Zhifeng Zhang ◽  
Madhuri Dey ◽  
Adomas Povilianskas ◽  
...  
Keyword(s):  
Self Assembly ◽  
Physical Mechanism ◽  
Single Cells ◽  
Three Dimensional ◽  
3D Bioprinting ◽  
Precise Control ◽  
3D Space ◽  
Magnitude Range ◽  
Wide Range ◽  
Order Of Magnitude

Three-dimensional (3D) bioprinting is an appealing approach for building tissues; however, bioprinting of mini-tissue blocks (i.e., spheroids) with precise control on their positioning in 3D space has been a major obstacle. Here, we unveil “aspiration-assisted bioprinting (AAB),” which enables picking and bioprinting biologics in 3D through harnessing the power of aspiration forces, and when coupled with microvalve bioprinting, it facilitated different biofabrication schemes including scaffold-based or scaffold-free bioprinting at an unprecedented placement precision, ~11% with respect to the spheroid size. We studied the underlying physical mechanism of AAB to understand interactions between aspirated viscoelastic spheroids and physical governing forces during aspiration and bioprinting. We bioprinted a wide range of biologics with dimensions in an order-of-magnitude range including tissue spheroids (80 to 600 μm), tissue strands (~800 μm), or single cells (electrocytes, ~400 μm), and as applications, we illustrated the patterning of angiogenic sprouting spheroids and self-assembly of osteogenic spheroids.

DABCO-Directed Self-Assembly of Pyrene-Centered Porphyrin Pentamers

2013 ◽  
Vol 575-576 ◽  
pp. 123-129
Author(s):  
Zhuang Dong Yuan ◽  
Jing Xia Wang ◽  
Ning Sheng
Keyword(s):  
Self Assembly ◽  
Fluorescence Emission ◽  
The Self ◽  
Spectroscopic Methods ◽  
Assembly Process ◽  
Zinc Porphyrin ◽  
Axial Coordination ◽  
H Nmr ◽  
Wide Range ◽  
Nmr Techniques

DABCO (1, 4-diazabicyclo [2.2.2] octane) has been used in combination with pentameric zinc porphyrin-pyrene array 1 to form well-defined supramolecular arrays through axial coordination. The self-assembly process has been investigated by a wide range of spectroscopic methods including UV-vis, fluorescence emission and 1H NMR techniques.

Force-Assembled Fe3O4 Particle Chains in Polyurethane Matrix

2016 ◽  
Vol 851 ◽  
pp. 221-225
Author(s):  
Marek Zboncak ◽  
Frantisek Ondreas ◽  
Josef Jancar
Keyword(s):  
Magnetic Field ◽  
Self Assembly ◽  
Spatial Arrangement ◽  
Precise Control ◽  
Electro Magnetic Field ◽  
Wide Range ◽  
Structural Variables ◽  
Simple Change ◽  
Electro Magnetic ◽  
Polyurethane Matrix

Despite substantial research efforts, the potential of polymer nanocomposites has still not been fully revealed, mainly due to poor control over the dispersion and alignment of nanoparticles (NPs). Since nanocomposite properties are controlled by the structural variables, it is crucial to achieve control over the NP assembly process.Self-assembly of NPs offers limited control over the NP spatial arrangement. This process results in a poorly controlled variation of simple structures such as agglomerates, clusters and dispersed NPs with the resulting structure strongly dependent a on wide range of thermodynamic parameters.On the other hand, force-assembly exploits interactions between particles induced by external force fields overcoming the thermodynamic ones. Stimulus of external electric, magnetic or electro-magnetic field is applied as the main force controlling the assembly of NPs. Understanding this process gives us the opportunity to create prescribed NP structures with controlled shape, size, and anisotropy by simple change of the force field. Precise control of structure formation on different length scales (from nanoto macro) gives us the opportunity to imitate hierarchical biological structures possessing unique balance of stiffness and toughness.Here, we report on magnetic field force assembly of Fe3O4 nanoparticles in the polyurethane matrix. Resulting NP chain structures were several NP wide and tens of micrometers long aligned along the magnetic force lines. Without the magnetic field, NP agglomerates of random size and shape were formed due to their self-assembly.

scholarly journals One-pot synthesis of oxidation-sensitive supramolecular gels and vesicles

2021 ◽  
Author(s):  
Aroa Duro-Castano ◽  
Laura Rodriguez-Arco ◽  
Lorena Ruiz-Perez ◽  
Cesare De Pace ◽  
Gabriele Marchello ◽  
...  
Keyword(s):  
Self Assembly ◽  
Biomedical Applications ◽  
Stimuli Responsive ◽  
Sustained Drug Release ◽  
One Pot ◽  
Physiological Conditions ◽  
One Pot Synthesis ◽  
Wide Range ◽  
Spherical Micelles ◽  
The One

Polypeptide-based nanoparticles offer unique advantages from a nanomedicine perspective such as biocompatibility, biodegradability and stimuli-responsive properties to (patho)physiological conditions. Conventionally, self-assembled polypeptide nanostructures are prepared by first synthesizing their constituent amphiphilic polypeptides followed by post-polymerization self-assembly. Herein, we describe the one-pot synthesis of oxidation-sensitive supramolecular micelles and vesicles. This was achieved by polymerization-induced self-assembly (PISA) of the N-carboxyanhydride (NCA) precursor of methionine using polyethylene oxide as stabilizing and hydrophilic block in dimethyl sulfoxide (DMSO). By adjusting the hydrophobic block length and concentration we obtained a range of morphologies from spherical to worm-like micelles, to vesicles. Remarkably, the secondary structure of polypeptides greatly influenced the final morphology of the assemblies. Surprisingly, worm-like micellar morphologies were obtained for a wide range of methionine block lengths and solid contents, with spherical micelles restricted to very short hydrophobic lengths. Worm-like micelles further assembled into oxidation-sensitive, self-standing gels in the reaction pot. Both vesicles and worm-like micelles obtained using this method demonstrated to degrade under controlled oxidant conditions which would expand their biomedical applications such as in sustained drug release or as cellular scaffolds in tissue engineering.

Self-Assembly of Peptide Amphiphiles: Molecularly Engineered Bionanomaterials

2015 ◽  
Vol 1113 ◽  
pp. 586-593 ◽  
Author(s):  
Hamizah Shamsudeen ◽  
Huey Ling Tan
Keyword(s):  
Self Assembly ◽  
Building Blocks ◽  
Molecular Engineering ◽  
Molecular Structures ◽  
Significant Advance ◽  
Polymer Science ◽  
New Approach ◽  
Dna Structures ◽  
Self Assembling ◽  
Wide Range

Molecular self-assembly is ubiquitous in nature and has now emerged as a new approach in chemical synthesis, engineering, nanotechnology, polymer science, and materials. Molecular self-assembly has been attracting increasing interest from the scientific community in the recent years due to its importance in understanding biology and a variety of diseases at the molecular level. In the last few years, considerable advances have been made in the use of peptides as building blocks to produce biological materials for wide range of applications, including fabricating novel supra-molecular structures and scaffolding for tissue repair. Today, the study of biological self-assembly systems represent a significant advance in the molecular engineering and is a rapidly growing scientific and engineering field that crosses the boundaries of existing disciplines. Many self-assembling systems are range from bi-and tri-block copolymers to complex DNA structures as well as simple and complex proteins and peptides. The attractiveness of such bottom-up processes lies in their capability to build uniform, functional units or arrays and the possibility to exploit such structures at meso-and macroscopic scale for life and non-life science applications.

scholarly journals Nanoparticles Coated with Cell Membranes for Biomedical Applications

Biology ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 406 ◽  
Author(s):  
Carla Jiménez-Jiménez ◽  
Miguel Manzano ◽  
María Vallet-Regí
Keyword(s):  
Biomedical Applications ◽  
Cell Membranes ◽  
Scientific Research ◽  
Research Groups ◽  
New Developments ◽  
New Approach ◽  
Wide Range ◽  
Different Types ◽  
Near Future

Nanoparticles designed for diagnosing and treating different diseases have impacted the scientific research in biomedicine, and are expected to revolutionize the clinic in the near future through a new area called nanomedicine. In the last few years, a new approach in this field has emerged: the use of cell membranes for coating nanoparticles in an attempt to mimic the ability of cells to interface and interact with physiological environments. Although such functions have been replicated through synthetic techniques, many research groups are now employing naturally derived cell membranes to coat different types of nanoparticles in an attempt to improve their performance for a wide range of applications. This review summarizes the literature on nanoparticles coated with cell membranes and, more importantly, aims at inspiring and encouraging new developments to this technology in the biomedical area.

The non-innocent nature of graphene oxide as a theranostic platform for biomedical applications and its reactivity towards metal-based anticancer drugs

RSC Advances ◽  
2015 ◽  
Vol 5 (93) ◽  
pp. 76556-76566 ◽  
Author(s):  
Audrey Mokdad ◽  
Konstantinos Dimos ◽  
Giorgio Zoppellaro ◽  
Jiri Tucek ◽  
Jason A. Perman ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Anticancer Drugs ◽  
Self Assembly ◽  
Biomedical Applications ◽  
Anticancer Agent ◽  
The Self ◽  
Assembly Process ◽  
Mononuclear Iron

The self-assembly process of a mononuclear iron(ii) complex as anticancer agent with graphene oxide (GO) unveils the ability of GO to oxidize the metal drug.

A new approach to the preparation of poly(p-phenylene terephthalamide) nanofibers

RSC Advances ◽  
2016 ◽  
Vol 6 (32) ◽  
pp. 26599-26605 ◽  
Author(s):  
Hongchen Yan ◽  
Jinglong Li ◽  
Wenting Tian ◽  
Lianyuan He ◽  
Xinlin Tuo ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Self Assembly ◽  
Assembly Process ◽  
New Approach ◽  
Methoxy Polyethylene Glycol

Poly(p-phenylene terephthalamide) nanofibers were prepared via a polymerization induced self-assembly process with the assistance of methoxy polyethylene glycol (mPEG).

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