scholarly journals Effect of Ink and Pretreatment Conditions on Bioethanol and Biomethane Yields from Waste Banknote Paper

Polymers ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 239
Author(s):  
Omid Yazdani Aghmashhadi ◽  
Lisandra Rocha-Meneses ◽  
Nemailla Bonturi ◽  
Kaja Orupõld ◽  
Ghasem Asadpour ◽  
...  
Keyword(s):  
Environmental Impacts ◽  
Banking Industry ◽  
Ethanol Concentration ◽  
Biogas Production ◽  
Value Added ◽  
Raw Material ◽  
Acid Pretreatment ◽  
Different Temperatures ◽  
Pretreatment Conditions ◽  
Value Added Products

Waste banknote paper is a residue from the banking industry that cannot be recycled due to the presence of ink, microbial load and special coating that provides protection against humidity. As a result, waste banknote paper ends up being burned or buried, which brings environmental impacts, mainly caused by the presence of heavy metals in its composition. To minimize the environmental impacts that come from the disposal of waste banknote paper, this study proposes to produce value-added products (bioethanol and biogas) from waste banknote paper. For this, the effect of ink and pretreatment conditions on bioethanol and biomethane yields were analyzed. Waste banknote paper provided by the Central Bank of Iran was used. The raw material with ink (WPB) and without ink (WPD) was pretreated using sulfuric acid at different concentrations (1%, 2%, 3%, and 4%) and the nitrogen explosive decompression (NED) at different temperatures (150 °C, 170 °C, 190 °C, and 200 °C). The results show that the use of NED pretreatment in WPD resulted in the highest glucose concentration of all studies (13 ± 0.19 g/L). The acid pretreatment for WPB showed a correlation with the acid concentration. The highest ethanol concentration was obtained from the fermentation using WPD pretreated with NED (6.36 ± 0.72 g/L). The maximum methane yields varied between 136 ± 5 mol/kg TS (2% acid WPB) and 294 ± 4 mol/kg TS (3% acid WPD). Our results show that the presence of ink reduces bioethanol and biogas yields and that the chemical-free NED pretreatment is more advantageous for bioethanol and biogas production than the acid pretreatment method. Waste banknote paper without ink is a suitable feedstock for sustainable biorefinery processes.

Revalorization of Sunflower Stalk Pitch As Feedstock for the Coproduction of Pectin and Glucose Using a Two-Step Dilute Acid Pretreatment Process

2021 ◽  
Author(s):  
Qibo Zhang ◽  
Xutong Ma ◽  
Xin Zhou ◽  
Yong Xu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Value Added ◽  
Raw Material ◽  
Dilute Acid Pretreatment ◽  
Dilute Acid ◽  
Pectin Degradation ◽  
Acid Pretreatment ◽  
Hydrolysis Method ◽  
Sulfuric Acid Treatment ◽  
Value Added Products

Abstract Background: Sunflower stalk pith, residue from the processing of sunflower, is rich in pectin and cellulose, thereby acting as an economic raw material for the acquisition of these compounds. In order to increase the commercial value of sunflower processing industry, a two-step sequential dilute sulfuric acid treatment combined with subsequent enzymatic hydrolysis was conducted on spent sunflower stalk pith to obtain the value-added products, pectin and glucose. Results: In this study, pectin was firstly extracted with a mild condition to avoid pectin degradation, which conducted at 95℃ with a pH of 2.0 for 2 h, and approximately 0.12 g/g of pectin could be recovered. Then the remaining solids followed by extracted pectin were subjected to the reinforced treatment process with 0.75% H2SO4 at 150 oC for 30 min to further improving enzymatic hydrolysis efficiency. Moreover, a fed-batch enzymatic hydrolysis was successfully performed with a solid 16% content, the glucose titer reached 103.1 g/L with a yield of 83.6 %.Conclusion: Finally, approximately 140 g pectin and 260 g glucose were produced from 1 kg of raw sunflower stalk pith using the integrated biorefinery process. This work put forward a two-step dilute acid pretreatment combined with enzymatic hydrolysis method to produce pectin and glucose from sunflower spent waste.

scholarly journals β-Galactosidases from a Sequence-Based Metagenome: Cloning, Expression, Purification and Characterization

2020 ◽  
Vol 9 (1) ◽  
pp. 55
Author(s):  
María Florencia Eberhardt ◽  
José Matías Irazoqui ◽  
Ariel Fernando Amadio
Keyword(s):  
Bacterial Species ◽  
Value Added ◽  
Raw Material ◽  
Activity Levels ◽  
Treatment Technology ◽  
Catalytic Domains ◽  
Stabilization Ponds ◽  
Cazy Database ◽  
Genes Encoding ◽  
Value Added Products

Stabilization ponds are a common treatment technology for wastewater generated by dairy industries. Large proportions of cheese whey are thrown into these ponds, creating an environmental problem because of the large volume produced and the high biological and chemical oxygen demands. Due to its composition, mainly lactose and proteins, it can be considered as a raw material for value-added products, through physicochemical or enzymatic treatments. β-Galactosidases (EC 3.2.1.23) are lactose modifying enzymes that can transform lactose in free monomers, glucose and galactose, or galactooligosacharides. Here, the identification of novel genes encoding β-galactosidases, identified via whole-genome shotgun sequencing of the metagenome of dairy industries stabilization ponds is reported. The genes were selected based on the conservation of catalytic domains, comparing against the CAZy database, and focusing on families with β-galactosidases activity (GH1, GH2 and GH42). A total of 394 candidate genes were found, all belonging to bacterial species. From these candidates, 12 were selected to be cloned and expressed. A total of six enzymes were expressed, and five cleaved efficiently ortho-nitrophenyl-β-galactoside and lactose. The activity levels of one of these novel β-galactosidase was higher than other enzymes reported from functional metagenomics screening and higher than the only enzyme reported from sequence-based metagenomics. A group of novel mesophilic β-galactosidases from diary stabilization ponds’ metagenomes was successfully identified, cloned and expressed. These novel enzymes provide alternatives for the production of value-added products from dairy industries’ by-products.

scholarly journals Optimization of a pretreatment and hydrolysis process for the efficient recovery of recycled sugars and unknown compounds from agricultural sweet sorghum bagasse stem pith solid waste

PeerJ ◽  
2019 ◽  
Vol 6 ◽  
pp. e6186 ◽  
Author(s):  
Ting-Ting Jiang ◽  
Yan Liang ◽  
Xiang Zhou ◽  
Zi-Wei Shi ◽  
Zhi-Jun Xin
Keyword(s):  
Solid Waste ◽  
Sweet Sorghum ◽  
Agricultural Waste ◽  
Value Added ◽  
Raw Material ◽  
Alkali Concentration ◽  
Sweet Sorghum Bagasse ◽  
Amorphous Cellulose ◽  
Sorghum Bagasse ◽  
Value Added Products

Background Sweet sorghum bagasse (SSB), comprising both a dermal layer and pith, is a solid waste generated by agricultural activities. Open burning was previously used to treat agricultural solid waste but is harmful to the environment and human health. Recent reports showed that certain techniques can convert this agricultural waste into valuable products. While SSB has been considered an attractive raw material for sugar extraction and the production of value-added products, the pith root in the SSB can be difficult to process. Therefore, it is necessary to pretreat bagasse before conventional hydrolysis. Methods A thorough analysis and comparison of various pretreatment methods were conducted based on physicochemical and microscopic approaches. The responses of agricultural SSB stem pith with different particle sizes to pretreatment temperature, acid and alkali concentration and enzyme dosage were investigated to determine the optimal pretreatment. The integrated methods are beneficial to the utilization of carbohydrate-based and unknown compounds in agricultural solid waste. Results Acid (1.5−4.5%, v/v) and alkali (5−8%, w/v) reagents were used to collect cellulose from different meshes of pith at 25–100 °C. The results showed that the use of 100 mesh pith soaked in 8% (w/v) NaOH solution at 100 °C resulted in 32.47% ± 0.01% solid recovery. Follow-up fermentation with 3% (v/v) acid and 6.5% (w/v) alkali at 50 °C for enzymolysis was performed with the optimal enzyme ratio. An analysis of the surface topography and porosity before and after pretreatment showed that both the pore size of the pith and the amount of exposed cellulose increased as the mesh size increased. Interestingly, various compounds, including 42 compounds previously known to be present and 13 compounds not previously known to be present, were detected in the pretreatment liquid, while 10 types of monosaccharides, including D-glucose, D-xylose and D-arabinose, were found in the enzymatic solution. The total monosaccharide content of the pith was 149.48 ± 0.3 mg/g dry matter. Discussion An integrated technique for obtaining value-added products from sweet sorghum pith is presented in this work. Based on this technique, lignin and hemicellulose were effectively broken down, amorphous cellulose was obtained and all sugars in the sweet sorghum pith were hydrolysed into monosaccharides. A total of 42 compounds previously found in these materials, including alcohol, ester, acid, alkene, aldehyde ketone, alkene, phenolic and benzene ring compounds, were detected in the pretreatment pith. In addition, several compounds that had not been previously observed in these materials were found in the pretreatment solution. These findings will improve the transformation of lignocellulosic biomass into sugar to create a high-value-added coproduct during the integrated process and to maximize the potential utilization of agricultural waste in current biorefinery processing.

Whey Utilization: Sustainable Uses and Environmental Approach

2021 ◽  
Vol 59 (2) ◽  
Author(s):  
Elizabeta Zandona ◽  
Marijana Blažić ◽  
Anet Režek Jambrak
Keyword(s):  
Whey Proteins ◽  
Organic Matter Content ◽  
Value Added ◽  
Edible Films ◽  
Matter Content ◽  
Raw Material ◽  
Sustainable Utilization ◽  
The Past ◽  
Food Applications ◽  
Value Added Products

The dairy industry produces large amounts of whey as a by- product or co-product, which has led to considerable environmental problems due to its high organic matter content. Over the past decades, possibilities of more environmentally and economically efficient whey utilisation have been studied, primarily to convert unwanted end products into a valuable raw material. Sustainable whey management is mostly oriented to biotechnological and food applications for the development of value-added products such as whey powders, whey proteins, functional food and beverages, edible films and coatings, lactic acid and other biochemicals, bioplastic, biofuels and similar valuable bioproducts. This paper provides an overview of the sustainable utilization of whey and its constituents, considering new refining approaches and integrated processes to covert whey, or lactose and whey proteins to high value-added whey-based products.

scholarly journals Exploiting xylan as sugar donor for the synthesis of an antiproliferative xyloside using an enzyme cascade

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Manuel Nieto-Domínguez ◽  
José Alberto Martínez-Fernández ◽  
Beatriz Fernández de Toro ◽  
Juan A. Méndez-Líter ◽  
Francisco Javier Cañada ◽  
...  
Keyword(s):  
Chemical Synthesis ◽  
Biomass Conversion ◽  
Value Added ◽  
Cost Effective ◽  
Raw Material ◽  
Birchwood Xylan ◽  
Enzyme Cascade ◽  
Antiproliferative Agent ◽  
Xylanolytic Enzymes ◽  
Value Added Products

Abstract Background Currently, industrial societies are seeking for green alternatives to conventional chemical synthesis. This demand has merged with the efforts to convert lignocellulosic biomass into value-added products. In this context, xylan, as one of main components of lignocellulose, has emerged as a raw material with high potential for advancing towards a sustainable economy. Results In this study, the recombinant endoxylanase rXynM from the ascomycete Talaromyces amestolkiae has been heterologously expressed in Pichia pastoris and used as one of the catalysts of an enzyme cascade developed to synthesize the antiproliferative 2-(6-hydroxynaphthyl) β-d-xylopyranoside, by transglycosylation of 2,6-dihydroxynaphthalene. The approach combines the use of two fungal xylanolytic enzymes, rXynM and the β-xylosidase rBxTW1 from the same fungus, with the cost-effective substrate xylan. The reaction conditions for the cascade were optimized by a Central Composite Design. Maximal productions of 0.59 and 0.38 g/L were reached using beechwood xylan and birchwood xylan, respectively. For comparison, xylans from other sources were tested in the same reaction, suggesting that a specific optimization is required for each xylan variety. The results obtained using this enzyme cascade and xylan were similar or better to those previously reported for a single catalyst and xylobiose, an expensive sugar donor. Conclusions Beechwood and birchwood xylan, two polysaccharides easily available from biomass, were used in a novel enzyme cascade to synthetize an antiproliferative agent. The approach represents a green alternative to the conventional chemical synthesis of 2-(6-hydroxynaphthyl) β-d-xylopyranoside using a cost-effective substrate. The work highlights the role of xylan as a raw material for producing value-added products and the potential of fungal xylanolytic enzymes in the biomass conversion.

scholarly journals A Review on Bioconversion of Agro-Industrial Wastes to Industrially Important Enzymes

2018 ◽  
Vol 5 (4) ◽  
pp. 93 ◽  
Author(s):  
Rajeev Ravindran ◽  
Shady Hassan ◽  
Gwilym Williams ◽  
Amit Jaiswal
Keyword(s):  
Solid Phase ◽  
Agricultural Waste ◽  
Large Fraction ◽  
Value Added ◽  
Industrial Wastes ◽  
Raw Material ◽  
Corn Cob ◽  
Phase Culture ◽  
Commercially Important ◽  
Value Added Products

Agro-industrial waste is highly nutritious in nature and facilitates microbial growth. Most agricultural wastes are lignocellulosic in nature; a large fraction of it is composed of carbohydrates. Agricultural residues can thus be used for the production of various value-added products, such as industrially important enzymes. Agro-industrial wastes, such as sugar cane bagasse, corn cob and rice bran, have been widely investigated via different fermentation strategies for the production of enzymes. Solid-state fermentation holds much potential compared with submerged fermentation methods for the utilization of agro-based wastes for enzyme production. This is because the physical–chemical nature of many lignocellulosic substrates naturally lends itself to solid phase culture, and thereby represents a means to reap the acknowledged potential of this fermentation method. Recent studies have shown that pretreatment technologies can greatly enhance enzyme yields by several fold. This article gives an overview of how agricultural waste can be productively harnessed as a raw material for fermentation. Furthermore, a detailed analysis of studies conducted in the production of different commercially important enzymes using lignocellulosic food waste has been provided.

scholarly journals Current Status of Microalgae to Produce Biogas through Pretreatment of Lignocellulosic Waste in the Era of 21-Century

2020 ◽  
Author(s):  
Ali Raza Ishaq ◽  
Faiza Jabeen ◽  
Maleeha Manzoor ◽  
Tahira Younis ◽  
Ayesha Noor ◽  
...  
Keyword(s):  
Fossil Fuels ◽  
Value Added ◽  
Raw Material ◽  
Current Status ◽  
Fish Feed ◽  
Potential Candidate ◽  
Lignocellulosic Waste ◽  
Major Drawback ◽  
Lignocellulosic Feedstocks ◽  
Value Added Products

Modern day civilization is dependent on energy generation by fossil fuels. But the major drawback of using fossil fuels is environmental pollution. Microalgae are potential candidate for production of various products of interest, such as proteins, mini food, pigments and triglycerides that can be converted into biofuels. Lignocellulosic feedstocks are the most abundantly available raw material of plants that can serve as a promising feedstock for cultivating bacteria, fungi, yeasts and microalgae to produce biofuels and other value-added products. Owing to the abundant availability of these low/no cost substrates, can be utilized as feedstocks for cultivating microalgae to generate biogas/biodiesel. Likewise, there is much room to exploit defatted algal biomass to be used as animal/fish feed and oil producing/accumulating genes knowledge in future to produce high and good quality biodiesel and biogas.

scholarly journals Improving Mildew Resistance of Soy Meal by Nano-Ag/TiO2, Zinc Pyrithione and 4-Cumylphenol

Polymers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 169 ◽  
Author(s):  
Wenping Li ◽  
Mingsong Chen ◽  
Yanchen Li ◽  
Jingmeng Sun ◽  
Yi Liu ◽  
...  
Keyword(s):  
Soy Protein ◽  
Cellular Structure ◽  
Value Added ◽  
Oil Industry ◽  
Raw Material ◽  
Zinc Pyrithione ◽  
Mildew Resistance ◽  
Soy Meal ◽  
Mold Resistance ◽  
Value Added Products

As a byproduct from the soybean oil industry, soy meal can be reproduced into value-added products to replace formaldehyde as a plywood adhesive. However, the use of soy meal has been limited by its poor antifungal and antiseptic properties. In this work, three kinds of material, namely nano-Ag/TiO2, zinc pyrithione, and 4-cumylphenol were applied to enhance the mildew resistance of soy meal via breakdown of the cellular structure of mildew. The fungi and mold resistance, morphology, thermal properties, and mechanism of the modified soy meal were evaluated. The success of the antifungal and antiseptic properties was confirmed by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy. The results indicated that all three kinds of material improved the fungi and mold resistance of soy meal, and sample B, which was modified with a compound of nano-Ag/TiO2 and zinc pyrithione, was the effective antifungal raw material for the soy-based adhesives. FTIR indicated that the great improvement of antifungal properties of soy meal modified with 4-cumylphenol might be caused by the reaction between COO– groups of soy protein. This research can help understand the effects of the chemical modification of nano-Ag/TiO2, zinc pyrithione, and 4-cumylphenol on soy meal, and the modified soy meal exhibits potential for utilization in the plywood adhesive industry.

scholarly journals Bamboo particleboards: recent developments

2019 ◽  
Vol 49 ◽  
Author(s):  
Christian Gauss ◽  
Victor De Araujo ◽  
Maristela Gava ◽  
Juliana Cortez-Barbosa ◽  
Holmer Savastano Junior
Keyword(s):  
Value Added ◽  
International Standards ◽  
Raw Material ◽  
Laminated Bamboo ◽  
Viable Solution ◽  
Recent Developments ◽  
Durable Products ◽  
Dimensional Variation ◽  
Support Research ◽  
Value Added Products

ABSTRACT Due to the high dimensional variation of bamboo, the manufacturing of materials such as plywood and laminated bamboo produces a high amount of residues. The production of particleboards could be used to overcome this problem and become a viable solution to reuse the generated waste as a raw material to high value-added products. This study aimed to present an overview of the bamboo particleboard production, as well as the mechanical and physical properties of this material, followed by a review of the advances in its research and development. In general, independently of the resin or bamboo species utilization, several bamboo particleboards meet the mechanical properties requirements of international standards for wood-based medium-density particleboards. The main focus of this study is to provide a review, in order to support research groups interested in using new bamboo-based materials for the development of manufactured durable products.

scholarly journals Potential Usage of Edible Mushrooms and Their Residues to Retrieve Valuable Supplies for Industrial Applications

2021 ◽  
Vol 7 (6) ◽  
pp. 427
Author(s):  
Harsh Kumar ◽  
Kanchan Bhardwaj ◽  
Ruchi Sharma ◽  
Eugenie Nepovimova ◽  
Natália Cruz-Martins ◽  
...  
Keyword(s):  
Agricultural Waste ◽  
Value Added ◽  
Edible Mushroom ◽  
Edible Films ◽  
Industrial Applications ◽  
Edible Mushrooms ◽  
Raw Material ◽  
Medicinal Properties ◽  
Disposal Problem ◽  
Value Added Products

Currently, the food and agricultural sectors are concerned about environmental problems caused by raw material waste, and they are looking for strategies to reduce the growing amount of waste disposal. Now, approaches are being explored that could increment and provide value-added products from agricultural waste to contribute to the circular economy and environmental protection. Edible mushrooms have been globally appreciated for their medicinal properties and nutritional value, but during the mushroom production process nearly one-fifth of the mushroom gets wasted. Therefore, improper disposal of mushrooms and untreated residues can cause fungal disease. The residues of edible mushrooms, being rich in sterols, vitamin D2, amino acids, and polysaccharides, among others, makes it underutilized waste. Most of the published literature has primarily focused on the isolation of bioactive components of these edible mushrooms; however, utilization of waste or edible mushrooms themselves, for the production of value-added products, has remained an overlooked area. Waste of edible mushrooms also represents a disposal problem, but they are a rich source of important compounds, owing to their nutritional and functional properties. Researchers have started exploiting edible mushroom by-products/waste for value-added goods with applications in diverse fields. Bioactive compounds obtained from edible mushrooms are being used in media production and skincare formulations. Furthermore, diverse applications from edible mushrooms are also being explored, including the synthesis of biosorbent, biochar, edible films/coating, probiotics, nanoparticles and cosmetic products. The primary intent of this review is to summarize the information related to edible mushrooms and their valorization in developing value-added products with industrial applications.

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