scholarly journals The Growth of 3T3 Fibroblasts on PHB, PLA and PHB/PLA Blend Films at Different Stages of Their Biodegradation In Vitro

Polymers ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 108
Author(s):  
Vsevolod A. Zhuikov ◽  
Elizaveta A. Akoulina ◽  
Dariana V. Chesnokova ◽  
You Wenhao ◽  
Tatiana K. Makhina ◽  
...  
Keyword(s):  
Polymer Films ◽  
Enzymatic Degradation ◽  
Dynamic Change ◽  
Hydrolytic Degradation ◽  
Degradation Process ◽  
Past Century ◽  
Scanning Calorimetry ◽  
Blend Films ◽  
3T3 Fibroblasts

Over the past century there was a significant development and extensive application of biodegradable and biocompatible polymers for their biomedical applications. This research investigates the dynamic change in properties of biodegradable polymers: poly(3-hydroxybutyrate (PHB), poly-l-lactide (PLA), and their 50:50 blend (PHB/PLA)) during their hydrolytic non-enzymatic (in phosphate buffered saline (PBS), at pH = 7.4, 37 °C) and enzymatic degradation (in PBS supplemented with 0.25 mg/mL pancreatic lipase). 3T3 fibroblast proliferation on the polymer films experiencing different degradation durations was also studied. Enzymatic degradation significantly accelerated the degradation rate of polymers compared to non-enzymatic hydrolytic degradation, whereas the seeding of 3T3 cells on the polymer films accelerated only the PLA molecular weight loss. Surprisingly, the immiscible nature of PHB/PLA blend (showed by differential scanning calorimetry) led to a slower and more uniform enzymatic degradation in comparison with pure polymers, PHB and PLA, which displayed a two-stage degradation process. PHB/PLA blend also displayed relatively stable cell viability on films upon exposure to degradation of different durations, which was associated with the uneven distribution of cells on polymer films. Thus, the obtained data are of great benefit for designing biodegradable scaffolds based on polymer blends for tissue engineering.

scholarly journals Synthesis, Characterization andIn VitroAnticancer Evaluation of Itaconic Acid Based Random Copolyester

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
J. Gowsika ◽  
R. Nanthini
Keyword(s):  
Itaconic Acid ◽  
Hydrolytic Degradation ◽  
Gel Permeation Chromatography ◽  
Xrd Analysis ◽  
Titanium Tetraisopropoxide ◽  
X Ray Diffraction ◽  
Scanning Calorimetry ◽  
H Nmr ◽  
Melt Polycondensation

The present study deals with the synthesis and characterization of an aliphatic copolyester, poly [butylene fumarate-co-butylene itaconate] (PIFB) copolymer was obtained from itaconic acid, fumaric acid, and 1,4-butanediol using titanium tetraisopropoxide (TTiPO) through a two step process of transesterification and melt polycondensation. The synthesized aliphatic random copolyester was characterized with the help of FT-IR,1H-NMR,13C-NMR, viscosity measurements, Gel Permeation Chromatography (GPC) and X-ray diffraction (XRD) analysis. Thermal properties have been analyzed using thermogravimetric analysis (TGA) and Differential Scanning Calorimetry (DSC). Hydrolytic degradation studies were carried out in acid and alkaline regions of various pH values. The synthesized copolymer was subjected toin vitroanticancer activity studies against human breast cancer (MCF-7) cell line.

scholarly journals Enzymatic degradation of poly(butylene succinate) copolyesters synthesized with the use of Candida antarctica lipase B

2018 ◽  
Author(s):  
Aleksandra Wcislek ◽  
Agueda Sonseca Olalla ◽  
Andrew McClain ◽  
Agnieszka Piegat ◽  
Peter Sobolewski ◽  
...  
Keyword(s):  
Mass Loss ◽  
Enzymatic Degradation ◽  
Degradation Process ◽  
Candida Antarctica ◽  
Degree Of Crystallinity ◽  
Surface Erosion ◽  
Fibrous Materials ◽  
Lipase B ◽  
Scanning Calorimetry ◽  
Butylene Succinate

<div><div><div><p>Abstract: Biodegradable polymers are an active area of investigation, particularly ones that can be produced from sustainable, biobased monomers, such as copolymers of poly(butylene succinate) (PBS). In this study, we examine the enzymatic degradation of poly(butylene succinate-dilinoleic succinate) (PBS-DLS) copolymers obtained by “green” enzymatic synthesis using lipase B from Candida antarctica (CALB). The copolymers differed in their hard to soft segments ratio, from 70:30 to 50:50 wt.%. Enzymatic degradation was carried out on electrospun membranes (scaffolds) and compression-moulded films using lipase fromPseudomomas cepacia. Poly(e-caprolactone) (PCL) was used as a reference aliphatic polyester. The degradation process was monitored gravimetrically via water uptake and mass loss. After 24 days, approx. 40% mass loss was observed for fibrous materials prepared from PBS-DLS 70:30 copolymer, as compared to approx. 10% mass loss for PBS-DLS 50:50. Infrared spectroscopy (FTIR) and SEC analysis were used to examine changes in chemical structure. Differential scanning calorimetry (DSC) and scanning light microscopy (LSM) revealed changes in degree of crystallinity, and changes in surface morphology, consistent with a surface erosion mechanism. We conclude that the obtained copolymers are suitable for tissue engineering applications thanks to tuneable degradation and lack of acidification during breakdown.</p></div></div></div>

scholarly journals Enzymatic degradation of poly(butylene succinate) copolyesters synthesized with the use of Candida antarctica lipase B

2018 ◽  
Author(s):  
Aleksandra Wcislek ◽  
Agueda Sonseca Olalla ◽  
Andrew McClain ◽  
Agnieszka Piegat ◽  
Peter Sobolewski ◽  
...  
Keyword(s):  
Mass Loss ◽  
Enzymatic Degradation ◽  
Degradation Process ◽  
Candida Antarctica ◽  
Degree Of Crystallinity ◽  
Surface Erosion ◽  
Fibrous Materials ◽  
Lipase B ◽  
Scanning Calorimetry ◽  
Butylene Succinate

<div><div><div><p>Abstract: Biodegradable polymers are an active area of investigation, particularly ones that can be produced from sustainable, biobased monomers, such as copolymers of poly(butylene succinate) (PBS). In this study, we examine the enzymatic degradation of poly(butylene succinate-dilinoleic succinate) (PBS-DLS) copolymers obtained by “green” enzymatic synthesis using lipase B from Candida antarctica (CALB). The copolymers differed in their hard to soft segments ratio, from 70:30 to 50:50 wt.%. Enzymatic degradation was carried out on electrospun membranes (scaffolds) and compression-moulded films using lipase fromPseudomomas cepacia. Poly(e-caprolactone) (PCL) was used as a reference aliphatic polyester. The degradation process was monitored gravimetrically via water uptake and mass loss. After 24 days, approx. 40% mass loss was observed for fibrous materials prepared from PBS-DLS 70:30 copolymer, as compared to approx. 10% mass loss for PBS-DLS 50:50. Infrared spectroscopy (FTIR) and SEC analysis were used to examine changes in chemical structure. Differential scanning calorimetry (DSC) and scanning light microscopy (LSM) revealed changes in degree of crystallinity, and changes in surface morphology, consistent with a surface erosion mechanism. We conclude that the obtained copolymers are suitable for tissue engineering applications thanks to tuneable degradation and lack of acidification during breakdown.</p></div></div></div>

scholarly journals Comparative Structure-Property Characterization of Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate)s Films under Hydrolytic and Enzymatic Degradation: Finding a Transition Point in 3-Hydroxyvalerate Content

Polymers ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 728 ◽  
Author(s):  
Vsevolod A. Zhuikov ◽  
Yuliya V. Zhuikova ◽  
Tatiana K. Makhina ◽  
Vera L. Myshkina ◽  
Alexey Rusakov ◽  
...  
Keyword(s):  
Molecular Mass ◽  
Enzymatic Degradation ◽  
Food Packaging ◽  
Hydrolytic Degradation ◽  
Analytical Techniques ◽  
Degree Of Crystallinity ◽  
Structure Property ◽  
Crystallinity Degree ◽  
Average Value

The hydrolytic and enzymatic degradation of polymer films of poly(3-hydroxybutyrate) (PHB) of different molecular mass and its copolymers with 3-hydroxyvalerate (PHBV) of different 3-hydroxyvalerate (3-HV) content and molecular mass, 3-hydroxy-4-methylvalerate (PHB4MV), and polyethylene glycol (PHBV-PEG) produced by the Azotobacter chroococcum 7B by controlled biosynthesis technique were studied under in vitro model conditions. The changes in the physicochemical properties of the polymers during their in vitro degradation in the pancreatic lipase solution and in phosphate-buffered saline for a long time (183 days) were investigated using different analytical techniques. A mathematical model was used to analyze the kinetics of hydrolytic degradation of poly(3-hydroxyaklannoate)s by not autocatalytic and autocatalytic hydrolysis mechanisms. It was also shown that the degree of crystallinity of some polymers changes differently during degradation in vitro. The total mass of the films decreased slightly up to 8–9% (for the high-molecular weight PHBV with the 3-HV content 17.6% and 9%), in contrast to the copolymer molecular mass, the decrease of which reached 80%. The contact angle for all copolymers after the enzymatic degradation decreased by an average value of 23% compared to 17% after the hydrolytic degradation. Young’s modulus increased up to 2-fold. It was shown that the effect of autocatalysis was observed during enzymatic degradation, while autocatalysis was not available during hydrolytic degradation. During hydrolytic and enzymatic degradation in vitro, it was found that PHBV, containing 5.7–5.9 mol.% 3-HV and having about 50% crystallinity degree, presents critical content, beyond which the structural and mechanical properties of the copolymer have essentially changed. The obtained results could be applicable to biomedical polymer systems and food packaging materials.

In vitro degradation of polyglycolic acid synthesized by a one-step reaction

2014 ◽  
Vol 34 (7) ◽  
pp. 591-596 ◽  
Author(s):  
Siye Tang ◽  
Guilian Li ◽  
Rui Zhang ◽  
Leilei Huang ◽  
Hui Tang
Keyword(s):  
Molecular Weight ◽  
Weight Loss ◽  
Melting Point ◽  
Hydrolytic Degradation ◽  
Chloroacetic Acid ◽  
Degradation Process ◽  
Polyglycolic Acid ◽  
Buffer Solution ◽  
One Step

Abstract Polyglycolic acid was synthesized by a one-step reaction of chloroacetic acid and triethylamine in tetrahydrofuran. The hydrolytic degradation of polyglycolic acid has been investigated. The in vitro degradation was investigated in a saline phosphate buffer. The degradation process was examined using Fourier transform infrared spectroscopy spectrum, weight loss, melting point, X-ray powder diffraction, pH, and scanning electron microscopy measurements. The chemical structure of polyglycolic acid has a small change during in vitro degradation. Degradation occurred with an increase in weight loss, a decrease in melting point, an increase in crystallinity during the initial degradation period and then a decrease afterwards. The melting point fell abruptly after the sixth week. This indicates that the degradation degree increased suddenly, i.e., the molecular weight of polyglycolic acid should decrease abruptly. The pH of the buffer solution fell quickly and decreased with time during in vitro degradation. The lower pH indicates that the molecular weight of polyglycolic acid synthesized by a one-step reaction should be low, and this may enable polyglycolic acid to degrade easily. As the degradation time increased, the surfaces of polyglycolic acid samples were highly degraded, and the surface porosity increased.

scholarly journals Enzymatic degradation of poly(butylene succinate) copolyesters synthesized with the use of Candida antarctica lipase B

2018 ◽  
Author(s):  
Aleksandra Wcislek ◽  
Agueda Sonseca Olalla ◽  
Andrew McClain ◽  
Agnieszka Piegat ◽  
Peter Sobolewski ◽  
...  
Keyword(s):  
Mass Loss ◽  
Enzymatic Degradation ◽  
Degradation Process ◽  
Candida Antarctica ◽  
Degree Of Crystallinity ◽  
Surface Erosion ◽  
Fibrous Materials ◽  
Lipase B ◽  
Scanning Calorimetry ◽  
Butylene Succinate

<div><div><div><p>Abstract: Biodegradable polymers are an active area of investigation, particularly ones that can be produced from sustainable, biobased monomers, such as copolymers of poly(butylene succinate) (PBS). In this study, we examine the enzymatic degradation of poly(butylene succinate-dilinoleic succinate) (PBS-DLS) copolymers obtained by “green” enzymatic synthesis using lipase B from Candida antarctica (CALB). The copolymers differed in their hard to soft segments ratio, from 70:30 to 50:50 wt.%. Enzymatic degradation was carried out on electrospun membranes (scaffolds) and compression-moulded films using lipase fromPseudomomas cepacia. Poly(e-caprolactone) (PCL) was used as a reference aliphatic polyester. The degradation process was monitored gravimetrically via water uptake and mass loss. After 24 days, approx. 40% mass loss was observed for fibrous materials prepared from PBS-DLS 70:30 copolymer, as compared to approx. 10% mass loss for PBS-DLS 50:50. Infrared spectroscopy (FTIR) and SEC analysis were used to examine changes in chemical structure. Differential scanning calorimetry (DSC) and scanning light microscopy (LSM) revealed changes in degree of crystallinity, and changes in surface morphology, consistent with a surface erosion mechanism. We conclude that the obtained copolymers are suitable for tissue engineering applications thanks to tuneable degradation and lack of acidification during breakdown.</p></div></div></div>

Enzymatic Degradation of Candida Antarctica Lipase B Catalyzed Poly(butylene Succinate) Copolyesters Containing Dimer Linoleic Diol

2018 ◽  
Author(s):  
Aleksandra Wcislek ◽  
Agueda Sonseca ◽  
Andrew McClain ◽  
Agnieszka Piegat ◽  
Peter Sobolewski ◽  
...  
Keyword(s):  
Mass Loss ◽  
Enzymatic Degradation ◽  
Degradation Process ◽  
Candida Antarctica ◽  
Degree Of Crystallinity ◽  
Surface Erosion ◽  
Fibrous Materials ◽  
Lipase B ◽  
Scanning Calorimetry ◽  
Butylene Succinate

<div><div><div><p>Biodegradable polymers are an active area of investigation, particularly ones that can be produced from sustainable, biobased monomers, such as copolymers of poly(butylene succinate) (PBS). In this study, we examine the enzymatic degradation of poly(butylene succinate-dilinoleic succinate) (PBS-DLS) copolymers obtained by “green” enzymatic synthesis using lipase B from Candida antarctica (CALB). The copolymers differed in their hard to soft segments ratio, from 70:30 to 50:50 wt.%. Enzymatic degradation was carried out on electrospun membranes (scaffolds) and compression-moulded films using lipase fromPseudomomas cepacia. Poly(e-caprolactone) (PCL) was used as a reference aliphatic polyester. The degradation process was monitored gravimetrically via water uptake and mass loss. After 24 days, approx. 40% mass loss was observed for fibrous materials prepared from PBS-DLS 70:30 copolymer, as compared to approx. 10% mass loss for PBS-DLS 50:50. Infrared spectroscopy (FTIR) and SEC analysis were used to examine changes in chemical structure. Differential scanning calorimetry (DSC) and scanning light microscopy (LSM) revealed changes in degree of crystallinity, and changes in surface morphology, consistent with a surface erosion mechanism. We conclude that the obtained copolymers are suitable for tissue engineering applications thanks to tuneable degradation and lack of acidification during breakdown.</p></div></div></div>

Release of Aspirin from Biodegradable Polyesterurethane Networks

2009 ◽  
Vol 79-82 ◽  
pp. 1431-1434 ◽  
Author(s):  
Ya Kai Feng ◽  
Shi Feng Zhang ◽  
Li Zhang ◽  
Jin Tang Guo ◽  
Yong Shen Xu
Keyword(s):  
Drug Release ◽  
Release Rate ◽  
In Vitro Release ◽  
Degradation Process ◽  
Crosslinking Density ◽  
Release Mechanism ◽  
Scanning Calorimetry ◽  
Model Drug ◽  
Phosphate Buffered Saline

In this paper, the release of model drug aspirin (ASP) from biodegradable polyesterurethane networks was studied. Poly(D,L-lactide-co-glycolide)urethane (PULG) networks were prepared from hydroxyl telechelic star-shaped oligo(D,L-lactide-co-glycolide) coupled with 1,6-diisocyanate-2,2,4-trimethylhexane and 1,6-diisocyanate-2,4,4-trimethylhexane or isophorone diisocyanate. PULG networks turned from transparent to opaque after ASP loading. PULG networks with lower crosslinking density always resulted in higher drug loaded content. The results of differential scanning calorimetry (DSC) and scanning electron microscope (SEM) measurements demonstrated that ASP was uniformly distributed in the networks. The drug release courses of ASP from PULG networks in phosphate buffered saline pH = 7.0 at 37 °C could be divided into three stages. Firstly, ASP release was at approximately uniform rate from PULG networks; Secondly, the release rate obviously increased for the degradation of the PULG networks; Thirdly, the release rate decreased gradually because most of the ASP had diffused out of the PULG networks. The crosslinking density of polyesterurethane networks also affected drug release rate. The in vitro release test revealed that ASP accelerated the degradation process of PULG, which exhibited a typical erosion-controlled release mechanism.

Fenofibrate Solid Dispersion for Improving Oral Bioavailability: Preparation, and Characterization and in vivo Evaluation

2020 ◽  
Vol 13 (5) ◽  
pp. 5102-5109
Author(s):  
Venu Madhav K ◽  
Somnath De ◽  
Chandra Shekar Bonagiri ◽  
Sridhar Babu Gummadi
Keyword(s):  
Oral Bioavailability ◽  
Oral Delivery ◽  
Solid Dispersions ◽  
In Vitro Release ◽  
Aqueous Solubility ◽  
Water Soluble ◽  
Scanning Calorimetry ◽  
In Vivo Evaluation

Fenofibrate (FN) is used in the treatment of hypercholesterolemia. It shows poor dissolution and poor oral bioavailability after oral administration due to high liphophilicity and low aqueous solubility. Hence, solid dispersions (SDs) of FN (FN-SDs) were develop that might enhance the dissolution and subsequently oral bioavailability. FN-SDs were prepared by solvent casting method using different carriers (PEG 4000, PEG 6000, β cyclodextrin and HP β cyclodextrin) in different proportions (0.25%, 0.5%, 0.75% and 1% w/v). FN-SDs were evaluated solubility, assay and in vitro release studies for the optimization of SD formulation. Differential scanning calorimetry (DSC), powder X-ray diffraction (PXRD) and scanning electron microscopy (SEM) analysis was performed for crystalline and morphology analysis, respectively. Further, optimized FN-SD formulation evaluated for pharmacokinetic performance in Wistar rats, in vivo in comparison with FN suspension.  From the results, FN-SD3 and FN-SD6 have showed 102.9 ±1.3% and 105.5±3.1% drug release, respectively in 2 h. DSC and PXRD studies revealed that conversion of crystalline to amorphous nature of FN from FT-SD formulation. SEM studies revealed the change in the orientation of FN when incorporated in SDs. The oral bioavailability FN-SD3 and FN-SD6 formulations exhibited 2.5-folds and 3.1-folds improvement when compared to FN suspension as control. Overall, SD of FN could be considered as an alternative dosage form for the enhancement of oral delivery of poorly water-soluble FN.

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