scholarly journals Effect of Iron-Oxide Nanoparticles Impregnated Bacterial Cellulose on Overall Properties of Alginate/Casein Hydrogels: Potential Injectable Biomaterial for Wound Healing Applications

Polymers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 2690
Author(s):  
Rahul Patwa ◽  
Oyunchimeg Zandraa ◽  
Zdenka Capáková ◽  
Nabanita Saha ◽  
Petr Sáha
Keyword(s):  
Wound Healing ◽  
Magnetic Properties ◽  
Bacterial Cellulose ◽  
Chemical Properties ◽  
Microscopic Analysis ◽  
Cross Linking ◽  
Mechanical Compression ◽  
Physico Chemical ◽  
Cytotoxicity Studies ◽  
Migration Of Cells

In this study we report the preparation of novel multicomponent hydrogels as potential biomaterials for injectable hydrogels comprised of alginate, casein and bacterial cellulose impregnated with iron nanoparticles (BCF). These hydrogels demonstrated amide cross-linking of alginate–casein, ionic cross-linking of alginate and supramolecular interaction due to incorporation of BCF. Incorporation of BCF into the hydrogels based on natural biopolymers was done to reinforce the hydrogels and impart magnetic properties critical for targeted drug delivery. This study aimed to improve overall properties of alginate/casein hydrogels by varying the BCF loading. The physico-chemical properties of gels were characterized via FTIR, XRD, DSC, TGA, VSM and mechanical compression. In addition, swelling, drug release, antibacterial activity and cytotoxicity studies were also conducted on these hydrogels. The results indicated that incorporation of BCF in alginate/casein hydrogels led to mechanically stronger gels with magnetic properties, increased porosity and hence increased swelling. A porous structure, which is essential for migration of cells and biomolecule transportation, was confirmed from microscopic analysis. The porous internal structure promoted cell viability, which was confirmed through MTT assay of fibroblasts. Moreover, a hydrogel can be useful for the delivery of essential drugs or biomolecules in a sustained manner for longer durations. These hydrogels are porous, cell viable and possess mechanical properties that match closely to the native tissue. Collectively, these hybrid alginate–casein hydrogels laden with BCF can be fabricated by a facile approach for potential wound healing applications.

scholarly journals How to Improve Physico-Chemical Properties of Silk Fibroin Materials for Biomedical Applications?—Blending and Cross-Linking of Silk Fibroin—A Review

Materials ◽  
2021 ◽  
Vol 14 (6) ◽  
pp. 1510
Author(s):  
Sylwia Grabska-Zielińska ◽  
Alina Sionkowska
Keyword(s):  
Silk Fibroin ◽  
Biomedical Applications ◽  
Magnetic Particles ◽  
Chemical Properties ◽  
Ternary Mixtures ◽  
Cross Linking ◽  
Advantages And Disadvantages ◽  
Physico Chemical ◽  
Binary And Ternary Mixtures ◽  
To Receive

This review supplies a report on fresh advances in the field of silk fibroin (SF) biopolymer and its blends with biopolymers as new biomaterials. The review also includes a subsection about silk fibroin mixtures with synthetic polymers. Silk fibroin is commonly used to receive biomaterials. However, the materials based on pure polymer present low mechanical parameters, and high enzymatic degradation rate. These properties can be problematic for tissue engineering applications. An increased interest in two- and three-component mixtures and chemically cross-linked materials has been observed due to their improved physico-chemical properties. These materials can be attractive and desirable for both academic, and, industrial attention because they expose improvements in properties required in the biomedical field. The structure, forms, methods of preparation, and some physico-chemical properties of silk fibroin are discussed in this review. Detailed examples are also given from scientific reports and practical experiments. The most common biopolymers: collagen (Coll), chitosan (CTS), alginate (AL), and hyaluronic acid (HA) are discussed as components of silk fibroin-based mixtures. Examples of binary and ternary mixtures, composites with the addition of magnetic particles, hydroxyapatite or titanium dioxide are also included and given. Additionally, the advantages and disadvantages of chemical, physical, and enzymatic cross-linking were demonstrated.

Analysis of the influence of synthetic paramaters on the structure and physico-chemical properties of non-spherical iron oxide nanocrystals and their biological stability and compatibility

2016 ◽  
Vol 45 (2) ◽  
pp. 797-810 ◽  
Author(s):  
Alberto Pardo ◽  
Rosa Pujales ◽  
Mateo Blanco ◽  
Eva M. Villar-Alvarez ◽  
Silvia Barbosa ◽  
...  
Keyword(s):  
Magnetic Properties ◽  
Iron Oxide ◽  
Chemical Properties ◽  
High Potential ◽  
Biological Stability ◽  
Physico Chemical ◽  
Theranostic Applications

Monodisperse non-spherical magnetic IONCs obtained by simple methods display excellent magnetic properties with high potential for theranostic applications.

scholarly journals Synthesis and Cytotoxicity Studies of Wood-Based Cationic Cellulose Nanocrystals as Potential Immunomodulators

Nanomaterials ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1603
Author(s):  
Yusha Imtiaz ◽  
Beza Tuga ◽  
Christopher W. Smith ◽  
Alexander Rabideau ◽  
Long Nguyen ◽  
...  
Keyword(s):  
Cellulose Nanocrystals ◽  
Chemical Properties ◽  
Neutral Red ◽  
Cationic Polymers ◽  
Experimental Conditions ◽  
Physiological Processes ◽  
Transmission Electron ◽  
Physico Chemical ◽  
Cytotoxicity Studies ◽  
Low Cytotoxicity

Polysaccharides have been shown to have immunomodulatory properties. Modulation of the immune system plays a crucial role in physiological processes as well as in the treatment and/or prevention of autoimmune and infectious diseases. Cellulose nanocrystals (CNCs) are derived from cellulose, the most abundant polysaccharide on the earth. CNCs are an emerging class of crystalline nanomaterials with exceptional physico-chemical properties for high-end applications and commercialization prospects. The aim of this study was to design, synthesize, and evaluate the cytotoxicity of a series of biocompatible, wood-based, cationic CNCs as potential immunomodulators. The anionic CNCs were rendered cationic by grafting with cationic polymers having pendant +NMe3 and +NH3 moieties. The success of the synthesis of the cationic CNCs was evidenced by Fourier transform infrared spectroscopy, dynamic light scattering, zeta potential, and elemental analysis. No modification in the nanocrystals rod-like shape was observed in transmission electron microscopy and atomic force microscopy analyses. Cytotoxicity studies using three different cell-based assays (MTT, Neutral Red, and LIVE/DEAD®) and three relevant mouse and human immune cells indicated very low cytotoxicity of the cationic CNCs in all tested experimental conditions. Overall, our results showed that cationic CNCs are suitable to be further investigated as immunomodulators and potential vaccine nanoadjuvants.

scholarly journals Preparation and of PVA-based compositions with embedded silver, copper and zinc oxide nanoparticles and assessment of their antibacterial properties

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Jolanta Pulit-Prociak ◽  
Anita Staroń ◽  
Paweł Staroń ◽  
Anna Chmielowiec-Korzeniowska ◽  
Agata Drabik ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Metallic Nanoparticles ◽  
Antibacterial Properties ◽  
Chemical Properties ◽  
Microscopic Analysis ◽  
Poly Vinyl Alcohol ◽  
Physico Chemical ◽  
Dynamic Light Scattering Technique ◽  
Ft Ir ◽  
Bacterial Film

Abstract A series of poly(vinyl alcohol) (PVA) based liquid compositions with addition of zinc oxide, silver and copper nanoparticles has been prepared. The compositions also contained other consistency-forming organic components. The physico-chemical properties of the products have been determined. Their pH and density have been assessed. Also, the size of nanoparticles has been defined with using a dynamic light scattering technique. The compositions were subjected to XRD, FT-IR and microscopic analysis as well. Thanks to the incorporation of both metal oxide and metallic nanoparticles, it was possible to enrich the products with antibacterial properties. Their inhibiting properties in the growth of microorganisms have been confirmed against both Gram-negative and Gram-positive strains such as E. coli, S. aureus and P. aeruginosa. Thanks to the ability for solidification, the compositions may be applied on a bacterially contaminated surface, and after destroying the microorganisms and its solidification, it may be peeled off along with the dead bacterial film.

scholarly journals Sterculia Gum: Chemical Structure, Composition and Physico-Chemical Properties

2019 ◽  
Vol 32 (1) ◽  
pp. 1-8
Author(s):  
N. Sharma ◽  
T. Sinderpal
Keyword(s):  
Drug Delivery ◽  
Wound Healing ◽  
Chemical Properties ◽  
High Water ◽  
Molecular Organization ◽  
Water Retention Capacity ◽  
Molecular Architecture ◽  
Retention Capacity ◽  
Gelling Agents ◽  
Physico Chemical

Physico-chemical properties are crucial characteristics of hydrocolloids as they decide the applicability of them. Rheology of system, flow behaviour and mechanical properties make hydrocolloids suitable for food industry. Modification of consistency or texture properties of functional polymers also controls their sensory characteristics, thereby they become significant essences such as thickener, gelling agents, foaming agent, texture modifier, viscosifier, emulsifier, stabilizer and binder. Industrial and pharmaceutical applications are also controlled by some suitable physico-chemical properties of hydrocolloids. The polysaccharide gum exudates constitute a architecturally distinct class of complex biomacromolecules having unique physico-chemical properties. Due to their good bio/tissue compatibility, non-toxicity, they are extensively used in the field of tissue engineering, drug delivery and wound healing. Chemical and molecular architecture of hydrocolloids in turn controls their physico-chemical and functional properties. Sterculia gum is a substituted rhamnogalacturonoglycan (pectic) type exudate gum used as suspending agent, gelling agents, emulsifier, bulk laxative, dental adhesive, drug delivery agent and wound healing agent. It exhibits high water retention capacity, high viscosity and least solubility. Solutions of sterculia gum are viscoelastic and thixotropic. Sterculia gum has been recommended as effective wound dressing material as it can form a intensely adhesive gel when dispersed in minimum ammount of water. Owing to wide applications and distinctive properties of sterculia gum, present work is an endeavor to summarize the molecular organization, chemical configuration and physico-chemical properties of sterculia gum and the factors affecting physico-chemical properties of sterculia gum.

scholarly journals The Structural and Magnetic Properties of FeII and CoII Complexes with 2-(furan-2-yl)-5-pyridin-2-yl-1,3,4-oxadiazole

Molecules ◽  
2020 ◽  
Vol 25 (2) ◽  
pp. 277 ◽  
Author(s):  
Pavel Zoufalý ◽  
Erik Čižmár ◽  
Juraj Kuchár ◽  
Radovan Herchel
Keyword(s):  
Magnetic Properties ◽  
Magnetic Measurements ◽  
Chemical Properties ◽  
Static Field ◽  
X Ray Diffraction ◽  
Paramagnetic Resonance ◽  
Physico Chemical ◽  
X Band ◽  
Spin Arrangement ◽  
Ft Ir

Two novel coordination compounds containing heterocyclic bidentate N,N-donor ligand 2-(furan-2-yl)-5-(pyridin-2-yl)-1,3,4-oxadiazole (fpo) were synthesized. A general formula for compounds originating from perchlorates of iron, cobalt, and fpo can be written as: [M(fpo)2(H2O)2](ClO4)2 (M = Fe(II) for (1) Co(II) for (2)). The characterization of compounds was performed by general physico-chemical methods—elemental analysis (EA), Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR) in case of organics, and single crystal X-ray diffraction (sXRD). Moreover, magneto-chemical properties were studied employing measurements in static field (DC) for 1 and X-band EPR (Electron paramagnetic resonance), direct current (DC), and alternating current (AC) magnetic measurements in case of 2. The analysis of DC magnetic properties revealed a high spin arrangement in 1, significant rhombicity for both complexes, and large magnetic anisotropy in 2 (D = −21.2 cm−1). Moreover, 2 showed field-induced slow relaxation of the magnetization (Ueff = 65.3 K). EPR spectroscopy and ab initio calculations (CASSCF/NEVPT2) confirmed the presence of easy axis anisotropy and the importance of the second coordination sphere.

scholarly journals Biomaterials with Potential Use in Bone Tissue Regeneration—Collagen/Chitosan/Silk Fibroin Scaffolds Cross-Linked by EDC/NHS

Materials ◽  
2021 ◽  
Vol 14 (5) ◽  
pp. 1105
Author(s):  
Sylwia Grabska-Zielińska ◽  
Alina Sionkowska ◽  
Ângela Carvalho ◽  
Fernando J. Monteiro
Keyword(s):  
Silk Fibroin ◽  
Three Dimensional ◽  
Chemical Properties ◽  
Biological Properties ◽  
Bone Tissue Regeneration ◽  
Cross Linking ◽  
Microscopy Imaging ◽  
Physico Chemical ◽  
Ethylcarbodiimide Hydrochloride ◽  
Scaffold Structure

Blending of different biopolymers, e.g., collagen, chitosan, silk fibroin and cross-linking modifications of these mixtures can lead to new materials with improved physico-chemical properties, compared to single-component scaffolds. Three-dimensional scaffolds based on three-component mixtures of silk fibroin, collagen and chitosan, chemically cross-linked, were prepared and their physico-chemical and biological properties were evaluated. A mixture of EDC (N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride) and NHS (N-hydroxysuccinimide) was used as a cross-linking agent. FTIR was used to observe the position of the peaks characteristic for collagen, chitosan and silk fibroin. The following properties depending on the scaffold structure were studied: swelling behavior, liquid uptake, moisture content, porosity, density, and mechanical parameters. Scanning Electron Microscopy imaging was performed. Additionally, the biological properties of these materials were assessed, by metabolic activity assay. The results showed that the three-component mixtures, cross-linked by EDC/NHS and prepared by lyophilization method, presented porous structures. They were characterized by a high swelling degree. The composition of scaffolds has an influence on mechanical properties. All of the studied materials were cytocompatible with MG-63 osteoblast-like cells.

scholarly journals Hyaluronic Acid Reduces Bacterial Fouling and Promotes Fibroblasts’ Adhesion onto Chitosan 2D-Wound Dressings

2020 ◽  
Vol 21 (6) ◽  
pp. 2070 ◽  
Author(s):  
Ilaria Silvestro ◽  
Mariangela Lopreiato ◽  
Anna Scotto d’Abusco ◽  
Valerio Di Lisio ◽  
Andrea Martinelli ◽  
...  
Keyword(s):  
Wound Healing ◽  
Hyaluronic Acid ◽  
Biofilm Formation ◽  
Transmission Rate ◽  
Wound Care ◽  
Chemical Properties ◽  
Infectious Complications ◽  
Wound Dressings ◽  
Physico Chemical ◽  
Wound Healing Activity

Wound healing is a dynamic process that can be seriously delayed by many factors including infectious complications. The development of dressings with intrinsic wound healing activity and/or releasing bioactive compounds may help with addressing such an issue. In this study, hyaluronic acid (HA) at different percentages (1–35%) was used to modify chitosan (CS) biological and physico-chemical properties in order to obtain 2D-matrices able to promote healing and protect from infection. HA incorporation in the CS matrix decreased film transparency and homogeneity, but improved film water uptake and surface wettability. The water vapor transmission rate (WVTR) increased up to a 5% HA content, where it reached the highest value (672 g/m2 day), and decreased for higher HA contents. At all of the tested HA concentrations, HA affected mechanical properties providing matrices more flexible than pure CS with benefit for wound care. Pure CS films permitted S. epidermidis adhesion and biofilm formation. That was not true for CS/HA matrices, where HA at concentrations equal to or greater than 5% was able to avoid S. epidermidis adhesion. Fibroblasts adhesion also took benefit from the HA presence in the film, especially at 5% content, where the best adhesion and proliferation was found.

scholarly journals Preparation and Characterization of TPP-Chitosan Crosslinked Scaffolds for Tissue Engineering

Materials ◽  
2020 ◽  
Vol 13 (16) ◽  
pp. 3577 ◽  
Author(s):  
Ilaria Silvestro ◽  
Iolanda Francolini ◽  
Valerio Di Lisio ◽  
Andrea Martinelli ◽  
Loris Pietrelli ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Reaction Times ◽  
Chemical Properties ◽  
Biological Properties ◽  
Cross Linking ◽  
Crosslinking Reaction ◽  
Porous Structures ◽  
Factors Affecting ◽  
Physico Chemical

Scaffolds are three-dimensional porous structures that must have specific requirements to be applied in tissue engineering. Therefore, the study of factors affecting scaffold performance is of great importance. In this work, the optimal conditions for cross-linking preformed chitosan (CS) scaffolds by the tripolyphosphate polyanion (TPP) were investigated. The effect on scaffold physico-chemical properties of different concentrations of chitosan (1 and 2% w/v) and tripolyphosphate (1 and 2% w/v) as well as of cross-linking reaction times (2, 4, or 8 h) were studied. It was evidenced that a low CS concentration favored the formation of three-dimensional porous structures with a good pore interconnection while the use of more severe conditions in the cross-linking reaction (high TPP concentration and crosslinking reaction time) led to scaffolds with a suitable pore homogeneity, thermal stability, swelling behavior, and mechanical properties, but having a low pore interconnectivity. Preliminary biocompatibility tests showed a good osteoblasts’ viability when cultured on the scaffold obtained by CS 1%, TPP 1%, and an 8-h crosslinking time. These findings suggest how modulation of scaffold cross-linking conditions may permit to obtain chitosan scaffold with properly tuned morphological, mechanical and biological properties for application in the tissue regeneration field.

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