scholarly journals Investigation of the Cytotoxicity of Electrospun Polysuccinimide-Based Fiber Mats

Polymers ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2324
Author(s):  
Kristof Molnar ◽  
Rita Varga ◽  
Benjamin Jozsa ◽  
Dora Barczikai ◽  
Eniko Krisch ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cell Line ◽  
Aspartic Acid ◽  
Phase Contrast ◽  
Low Pressure ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
Fiber Mats ◽  
Normal Morphology ◽  
Equilibrium Plasma

This study investigated cell viability in the presence of allylamine-modified and plasma-treated electrospun polysuccinimide fiber mats (PSI-AAmp). Low pressure non-equilibrium plasma was used for crosslinking the PSI-AAm. Comparison of FTIR and XPS analyses demonstrated that crosslinking occurred on the surface of the samples. Cell viability was investigated using the MG-63 osteosarcoma cell line and WST-1 viability reagent. Since PSI hydrolyzes to poly(aspartic acid) (PASP), PASP was used in addition to the regular controls (cells only). Phase contrast showed normal morphology in all cases at 24 h; however, in the presence of PSI-AAmp at 72 h, some rounded, dead cells could also be seen, and proliferation was inhibited. Since proliferation in the presence of PASP alone was not inhibited, the cause of inhibition was not the final product of the hydrolysis. Further investigations will be carried out to pinpoint the cause.

scholarly journals VCP (p97) Regulates NFKB Signaling Pathway, Which Is Important for Metastasis of Osteosarcoma Cell Line

2002 ◽  
Vol 93 (3) ◽  
pp. 296-304 ◽  
Author(s):  
Tatsuya Asai ◽  
Yasuhiko Tomita ◽  
Shin-ichi Nakatsuka ◽  
Yoshihiko Hoshida ◽  
Akira Myoui ◽  
...  
Keyword(s):  
Cell Line ◽  
Signaling Pathway ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell

Recognition by cellular and humoral autologous immunity in a human osteosarcoma cell line

2003 ◽  
Vol 8 (4) ◽  
pp. 554-559 ◽  
Author(s):  
Yuki Nabeta ◽  
Kawaguchi Satoshi ◽  
Hiroeki Sahara ◽  
Hideyuki Ikeda ◽  
Yoshihiko Hirohashi ◽  
...  
Keyword(s):  
Cell Line ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
Human Osteosarcoma Cell Line ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cell

LRP8 activates STAT3 to induce PD-L1 expression in osteosarcoma

2020 ◽  
pp. 030089162095287
Author(s):  
Shiqin Zheng ◽  
Yuxi Wei ◽  
Yu Jiang ◽  
Yi Hao
Keyword(s):  
Cell Line ◽  
Culture System ◽  
Nuclear Translocation ◽  
Interleukin 2 ◽  
Dominant Negative ◽  
Jurkat Cells ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
Normal Tissues

Purpose: Targeting programmed death-ligand 1 (PD-L1) may be an effective intervention for osteosarcoma and PD-L1 expression is controlled by diverse regulatory factors. Low-density lipoprotein receptor-related protein 8 (LRP8) regulates osteoblast differentiation and it is unclear whether and how LRP8 could contribute to osteosarcoma pathogenesis. In this study, we investigated the LRP8/signal transducer and activator of transcription 3 (STAT3)/PD-L1 network in osteosarcoma. Methods: The expression of LRP8, STAT3, and PD-L1 was measured in osteosarcoma tissues and paired normal tissues. The effects of LRP8 on STAT3 and PD-L1 expression were investigated in an osteosarcoma cell line. The effects on immunosuppression were investigated in an in vitro co-culture system with Jurkat cell line and osteosarcoma cell line. The effects of LRP8 were blocked by a LRP8 neutralizing antibody, dominant-negative STAT3, or STAT3 inhibitor. Results: LRP8 was overexpressed in osteosarcoma compared to normal tissues and its level was correlated with phospho-STAT3 (p-STAT3) level in osteosarcoma tissues. In osteosarcoma cell lines, LRP8 increased p-STAT3 level and promoted nuclear translocation of STAT3. STAT3 activation also increased PD-L1 mRNA, protein, and promoter activity. In addition, LRP8 enhanced PD-L1 expression via STAT3. In a co-culture system, LRP8 overexpression in an osteosarcoma cell line impaired viability and interleukin-2 secretion of Jurkat cells and induced apoptosis of Jurkat cells. The effects of LRP8 could be blocked by neutralizing LRP8 antibody or STAT3 inhibitor. Blocking LRP8 inhibits proliferation and induces apoptosis of osteosarcoma cells. Conclusions: Our results provide evidence for a novel regulation network of LRP8/STAT3/PD-L1 in osteosarcoma and LRP8 may be a potential therapeutic target in osteosarcoma.

Inhibitory Effects of Indomethacin in Human MNNG/HOS Osteosarcoma Cell Line In Vitro

2019 ◽  
Vol 38 (1) ◽  
pp. 23-36 ◽  
Author(s):  
Saied Mirshahidi ◽  
Rosalia de Necochea-Campion ◽  
Annie Moretta ◽  
Nadine L. Williams ◽  
Mark E. Reeves ◽  
...  
Keyword(s):  
Cell Line ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
Inhibitory Effects
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