scholarly journals Natural Formulations Provide Antioxidant Complement to Hyaluronic Acid-Based Topical Applications Used in Wound Healing

Polymers ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 1847
Author(s):  
Pooyan Makvandi ◽  
Caterina Caccavale ◽  
Francesca Della Sala ◽  
Stefania Zeppetelli ◽  
Rosanna Veneziano ◽  
...  
Keyword(s):  
Wound Healing ◽  
Hyaluronic Acid ◽  
Vitamin E ◽  
Culture Dish ◽  
Rheological Characterization ◽  
Tissue Culture Dish ◽  
Biological Studies ◽  
A Cell ◽  
Topical Applications

Hyaluronic acid (HA) promotes wound healing, and, accordingly, formulations based on HA have been widely used in regenerative medicine. In addition, naturally derived compounds, e.g., plant-based extracts and vitamin E, have exhibited antioxidant activity. In this study, a formulation containing hyaluronic acid, vitamin E, raspberry extract, and green tea was developed for potential topical applications, targeting wound healing. Rheological analysis was performed along with antioxidant and biological studies. The rheological characterization showed that the HA-based formulation is a thixotropic platform and possesses higher mechanical properties than the control formulation. To evaluate the wound healing potential of the formulation, an in vitro “wound healing” assay was carried out using human derived fibroblasts (HDF) with a cell-free gap on the tissue culture dish. The formulation showed better wound healing ability than the control formulation.

scholarly journals Development of Spanish Broom and Flax Dressings with Glycyrrhetinic Acid-Loaded Films for Wound Healing: Characterization and Evaluation of Biological Properties

Pharmaceutics ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1192
Author(s):  
Angela Abruzzo ◽  
Concettina Cappadone ◽  
Valentina Sallustio ◽  
Giovanna Picone ◽  
Martina Rossi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Drug Loading ◽  
Healing Process ◽  
In Vitro Release ◽  
Sodium Hyaluronate ◽  
Biological Properties ◽  
Glycyrrhetinic Acid ◽  
Wound Dressings ◽  
Biological Studies

The selection of an appropriate dressing for each type of wound is a very important procedure for a faster and more accurate healing process. So, the aim of this study was to develop innovative Spanish Broom and flax wound dressings, as alternatives to cotton used as control, with polymeric films containing glycyrrhetinic acid (GA) to promote wound-exudate absorption and the healing process. The different wound dressings were prepared by a solvent casting method, and characterized in terms of drug loading, water uptake, and in vitro release. Moreover, biological studies were performed to evaluate their biocompatibility and wound-healing efficacy. Comparing the developed wound dressings, Spanish Broom dressings with GA-loaded sodium hyaluronate film had the best functional properties, in terms of hydration ability and GA release. Moreover, they showed a good biocompatibility, determining a moderate induction of cell proliferation and no cytotoxicity. In addition, the wound-healing test revealed that the Spanish Broom dressings promoted cell migration, further facilitating the closure of the wound.

Evaluation of characteristics and in vitro antioxidant properties of RSV loaded hyaluronic acid–DPPC microparticles as a wound healing system

2015 ◽  
Vol 126 ◽  
pp. 50-57 ◽  
Author(s):  
İpek Eroğlu ◽  
Evren H. Gökçe ◽  
Nicolas Tsapis ◽  
Sakine Tuncay Tanrıverdi ◽  
Göksel Gökçe ◽  
...  
Keyword(s):  
Wound Healing ◽  
Hyaluronic Acid ◽  
Antioxidant Properties ◽  
Healing System

Electrospun PVA/hyaluronic acid/L-arginine nanofibers for wound healing applications: Nanofibers optimization and in vitro bioevaluation

2020 ◽  
Vol 164 ◽  
pp. 667-676 ◽  
Author(s):  
Yasmein Hussein ◽  
Esmail M. El-Fakharany ◽  
Elbadawy A. Kamoun ◽  
Samah A. Loutfy ◽  
Rehab Amin ◽  
...  
Keyword(s):  
Wound Healing ◽  
Hyaluronic Acid

scholarly journals Human Keratinocyte Growth and Differentiation on Acellular Porcine Dermal Matrix in relation to Wound Healing Potential

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Robert Zajicek ◽  
Vaclav Mandys ◽  
Ondrej Mestak ◽  
Jan Sevcik ◽  
Radana Königova ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Keratinocytes ◽  
Dermal Matrix ◽  
Keratinocyte Proliferation ◽  
Proliferation And Differentiation ◽  
A Cell ◽  
Cell Culture Assay ◽  
Air Liquid Interface

A number of implantable biomaterials derived from animal tissues are now used in modern surgery. Xe-Derma is a dry, sterile, acellular porcine dermis. It has a remarkable healing effect on burns and other wounds. Our hypothesis was that the natural biological structure of Xe-Derma plays an important role in keratinocyte proliferation and formation of epidermal architecturein vitroas well asin vivo. The bioactivity of Xe-Derma was studied by a cell culture assay. We analyzed growth and differentiation of human keratinocytes culturedin vitroon Xe-Derma, and we compared the results with formation of neoepidermis in the deep dermal wounds treated with Xe-Derma. Keratinocytes cultured on Xe-Derma submerged in the culture medium achieved confluence in 7–10 days. After lifting the cultures to the air-liquid interface, the keratinocytes were stratified and differentiated within one week, forming an epidermis with basal, spinous, granular, and stratum corneum layers. Immunohistochemical detection of high-molecular weight cytokeratins (HMW CKs), CD29, p63, and involucrin confirmed the similarity of organization and differentiation of the cultured epidermal cells to the normal epidermis. The results suggest that the firm natural structure of Xe-Derma stimulates proliferation and differentiation of human primary keratinocytes and by this way improves wound healing.

scholarly journals Brain extract causes acetylcholine receptor redistribution which mimics some early events at developing neuromuscular junctions.

1982 ◽  
Vol 93 (2) ◽  
pp. 417-425 ◽  
Author(s):  
M M Salpeter ◽  
S Spanton ◽  
K Holley ◽  
T R Podleski
Keyword(s):  
Acetylcholine Receptors ◽  
Neuromuscular Junctions ◽  
Marked Change ◽  
Brain Extract ◽  
Bottom Surface ◽  
Culture Dish ◽  
Tissue Culture Dish ◽  
Em Autoradiography ◽  
Alpha Bungarotoxin

We studied the effect of rat brain extract on rat muscle cells in vitro by light and electron microscope (EM) autoradiography after labeling acetylcholine receptors (AChR's) with 125I-alpha-bungarotoxin. We found that: (a) In the absence of brain extract, peak site densities within AChR clusters usually do not exceed 4,000 sites/micrometer2. (b) Within hours after exposure to brain extract, AChR's redistribute to form clusters in which the peak site densities are greater than 10,000 sites/micrometer2. Receptor concentration within extract-induced clusters is thus within a factor of 2 of that at the neuromuscular junction (nmj). (c) In the absence of extract, the AChR's and AChR clusters are predominantly on the bottom surface of the myotubes (facing the tissue culture dish). After extract treatment, they are predominantly at the top surface. (d) Plasma membrane in regions of high-density AChR clusters is enriched in membrane with enhanced electron density and surface basal lamina whether or not cells are treated with extract. Extract causes an increase in both these specializations on the top surface of the myotubes. (e) Brain extract does not produce an overall increase in AChR site density or a marked change in degradation rate of receptors in either clustered or nonclustered regions. By producing AChR clusters with junctional site densities and enhanced surface specialization, and by causing an overall shift in AChR's distribution, brain extract mimics early events reported at developing neuromuscular junctions.

Wound healing of nanofiber comprising Polygalacturonic/Hyaluronic acid embedded silver nanoparticles: In-vitro and in-vivo studies

2020 ◽  
Vol 238 ◽  
pp. 116175 ◽  
Author(s):  
M.R. El-Aassar ◽  
Omar M. Ibrahim ◽  
Moustafa M.G. Fouda ◽  
Nagham G. El-Beheri ◽  
Mona M. Agwa
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
Hyaluronic Acid ◽  
In Vivo Studies

scholarly journals Wound Healing: In Vitro and In Vivo Evaluation of a Bio-Functionalized Scaffold Based on Hyaluronic Acid and Platelet-Rich Plasma in Chronic Ulcers

2019 ◽  
Vol 8 (9) ◽  
pp. 1486 ◽  
Author(s):  
Barbara De Angelis ◽  
Margarida Fernandes Lopes Morais D’Autilio ◽  
Fabrizio Orlandi ◽  
Giampiero Pepe ◽  
Simone Garcovich ◽  
...  
Keyword(s):  
Wound Healing ◽  
Hyaluronic Acid ◽  
Platelet Rich Plasma ◽  
Combined Treatment ◽  
Skin Grafting ◽  
Control Group ◽  
In Vivo Evaluation ◽  
Chronic Ulcers

Chronic ulcers are characterized by loss of substance without a normal tendency towards spontaneous healing. The Wound Bed Preparation Guideline advises that after diagnosis, the expert should correct the biological state of the ulcer micro-environment based on TIME principles (Tissue, Infection, Moisture balance, Epidermal). There are many ways to treat such ulcers, for example through use of advanced dressings, negative pressure, surgical toilets, dermal substitutes, autologous skin grafting, and free or local flaps. In vitro and in vivo pre-clinical models hold widely acknowledged potential yet complex limitations. Tissue bioengineering could be an ideal approach to foster innovative strategies in wound healing. Our observational study reports on an in vitro and in vivo evaluation of a bio-functionalized scaffold composed of platelet-rich plasma (PRP) and hyaluronic acid (HA) used in 182 patients affected by chronic ulcers (diabetic and vascular), comparing the results with a control group of 182 patients treated with traditional dressings (HA alone). After 30 days the patients who had undergone the combined treatment (PRP + HA), showed 96.8% ± 1.5% re-epithelialization, as compared to 78.4% ± 4.4% in the control group (HA only). Within 80 days, they had 98.4% ± 1.3% re-epithelialization as compared to 87.8% ± 4.1% in the control group (HA only; p < 0.05). No local recurrence was observed during the follow-up period. PRP + HA treatment showed stronger regenerative potential in terms of epidermal proliferation and dermal renewal compared with HA alone.

scholarly journals Combination of Two Kinds of Medicated Microparticles Based on Hyaluronic Acid or Chitosan for a Wound Healing Spray Patch

Pharmaceutics ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2195
Author(s):  
Angela Fabiano ◽  
Chiara Migone ◽  
Luca Cerri ◽  
Anna Maria Piras ◽  
Andrea Mezzetta ◽  
...  
Keyword(s):  
Wound Healing ◽  
Hyaluronic Acid ◽  
Second Life ◽  
Olive Leaves ◽  
Waste Products ◽  
Accelerate Wound Healing ◽  
Scratch Wound ◽  
Treatment Of Wounds ◽  
Scratch Wound Healing Assay

Olive leaves extract (OLE) has been extensively studied as antioxidant and antibiotic and these characteristics make it particularly interesting for use on wounds. For this reason, the aim of this study was to introduce OLE in microparticles (MP) of hyaluronic acid (MPHA-OLE) or chitosan (MPCs-OLE) to obtain a spray patch for the treatment of wounds in anatomical areas that are difficult to protect with traditional patches. The MP were characterized for particle size and ability to protect OLE from degradation, to absorb water from wound exudate, to control OLE release from MP. The MPHA and MPCs medicated or not and mixtures of the two types in different proportions were studied in vitro on fibroblasts by the scratch wound healing assay. The MP size was always less than 5 µm, and therefore, suitable for a spray patch. The MPCs-OLE could slow down the release of OLE therefore only about 60% of the polyphenols contained in it were released after 4 h. Both MPHA and MPCs could accelerate wound healing. A 50% MPHA-OLE-50% MPCs-OLE blend was the most suitable for accelerating wound healing. The MPHA-OLE-MPCs-OLE blends studied in this work were shown to have the characteristics suitable for a spray patch, thus giving a second life to the waste products of olive growers.

scholarly journals Thiolated Carboxymethyl-Hyaluronic-Acid-Based Biomaterials Enhance Wound Healing in Rats, Dogs, and Horses

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Guanghui Yang ◽  
Glenn D. Prestwich ◽  
Brenda K. Mann
Keyword(s):  
Wound Healing ◽  
Veterinary Medicine ◽  
Hyaluronic Acid ◽  
Wound Closure ◽  
Keratinocyte Proliferation ◽  
Accelerate Wound Healing ◽  
Multiple Species ◽  
Enhance Wound Healing

The progression of wound healing is a complicated but well-known process involving many factors, yet there are few products on the market that enhance and accelerate wound healing. This is particularly problematic in veterinary medicine where multiple species must be treated and large animals heal slower, oftentimes with complicating factors such as the development of exuberant granulation tissue. In this study a crosslinked-hyaluronic-acid (HA-) based biomaterial was used to treat wounds on multiple species: rats, dogs, and horses. The base molecule, thiolated carboxymethyl HA, was first found to increase keratinocyte proliferation in vitro. Crosslinked gels and films were then both found to enhance the rate of wound healing in rats and resulted in thicker epidermis than untreated controls. Crosslinked films were used to treat wounds on forelimbs of dogs and horses. Although wounds healed slower compared to rats, the films again enhanced wound healing compared to untreated controls, both in terms of wound closure and quality of tissue. This study indicates that these crosslinked HA-based biomaterials enhance wound healing across multiple species and therefore may prove particularly useful in veterinary medicine. Reduced wound closure times and better quality of healed tissue would decrease risk of infection and pain associated with open wounds.

scholarly journals Promotion of Keratinocyte Proliferation by Tracheloside through ERK1/2 Stimulation

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
JaeGoo Kim ◽  
Yu-Kyong Shin ◽  
Ki-Young Kim
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Positive Control ◽  
Skin Injury ◽  
Scratch Assay ◽  
Keratinocyte Proliferation ◽  
Promote Cell Migration ◽  
A Cell ◽  
Cell Migration And Proliferation

Cell migration and proliferation are important for proper wound healing after skin injury. Recent studies have shown that compounds from plants could promote cell migration and proliferation. Tracheloside, which is a plant lignan, has been found to promote the growth of HaCaT cells over 40% compared to other compounds tested based on a cell proliferation assay. An in vitro scratch assay confirmed the healing activity of tracheloside (more than 2-fold increased healing activity after 24 hours of treatment compared with the control) and revealed that this activity is better than that of allantoin (1.2-fold increased after 24 hours of treatment compared with the control), a positive control. With western blot results, wound healing with tracheloside occurred through the phosphorylation of ERK1/2. Therefore, tracheloside is a good candidate to promote wound healing and could be developed as a therapeutic agent for wound treatment or used as a leading compound with higher activity.

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