scholarly journals Production of Biodegradable Palm Oil-Based Polyurethane as Potential Biomaterial for Biomedical Applications

Polymers ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 1842
Author(s):  
Fang Hoong Yeoh ◽  
Choy Sin Lee ◽  
Yew Beng Kang ◽  
Shew Fung Wong ◽  
Sit Foon Cheng ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Palm Oil ◽  
Biomedical Applications ◽  
Enzymatic Degradation ◽  
Cytotoxicity Test ◽  
Adhesion Test ◽  
Polyester Polyol ◽  
Isocyanate Index ◽  
Soft Tissue Engineering ◽  
Cell Adhesion And Proliferation

Being biodegradable and biocompatible are crucial characteristics for biomaterial used for medical and biomedical applications. Vegetable oil-based polyols are known to contribute both the biodegradability and biocompatibility of polyurethanes; however, petrochemical-based polyols were often incorporated to improve the thermal and mechanical properties of polyurethane. In this work, palm oil-based polyester polyol (PPP) derived from epoxidized palm olein and glutaric acid was reacted with isophorone diisocyanate to produce an aliphatic polyurethane, without the incorporation of any commercial petrochemical-based polyol. The effects of water content and isocyanate index were investigated. The polyurethanes produced consisted of > 90% porosity with interconnected micropores and macropores (37–1700 µm) and PU 1.0 possessed tensile strength and compression stress of 111 kPa and 64 kPa. The polyurethanes with comparable thermal stability, yet susceptible to enzymatic degradation with 7–59% of mass loss after 4 weeks of treatment. The polyurethanes demonstrated superior water uptake (up to 450%) and did not induce significant changes in pH of the medium. The chemical changes of the polyurethanes after enzymatic degradation were evaluated by FTIR and TGA analyses. The polyurethanes showed cell viability of 53.43% and 80.37% after 1 and 10 day(s) of cytotoxicity test; and cell adhesion and proliferation in cell adhesion test. The polyurethanes produced demonstrated its potential as biomaterial for soft tissue engineering applications.

scholarly journals Surface properties and bioactivity of TiO 2 nanotube array prepared by two-step anodic oxidation for biomedical applications

2019 ◽  
Vol 6 (4) ◽  
pp. 181948 ◽  
Author(s):  
Zhaoxiang Peng ◽  
Jiahua Ni
Keyword(s):  
Cell Adhesion ◽  
Electron Microscope ◽  
Impedance Spectroscopy ◽  
Surface Energy ◽  
Anodic Oxidation ◽  
Biomedical Applications ◽  
Nanotube Array ◽  
Measuring Device ◽  
X Ray ◽  
Cell Adhesion And Proliferation

A highly ordered TiO 2 nanotube array has been prepared on a commercial pure titanium substrate in a hydrofluoric (HF) electrolyte using a DC power source through two-step anodic oxidation. The morphology, composition, wettability and surface energy of the nanotube array have been characterized by using a field-emission scanning electron microscope (FE-SEM), a transmission electron microscope (JEM-2010) with energy-dispersive X-ray spectrometer EDX (INCA OXFORD), X-ray diffraction method, an atomic force microscope (AFM), an optical contact angle measuring device and the Owens method with two liquids. The electrochemical behaviours of anodic oxidation films with different structures have been investigated in Sodium Lactate Ringer's Injection at 37±1°C by potentiodynamic polarization curve and electrochemical impedance spectroscopy. The formation mechanism of the nanotube array and the advantages of two-step oxidation have been discussed according to the experimental observation and the characterized results. Meanwhile, the structural changes of nanotubes are analysed according to the results of impedance spectroscopy. Cytotoxicity testing and cell adhesion and proliferation have been studied in order to evaluate the bioactivity of the nanotube array film. The diameters of nanotubes are in the range of 120–140 nm. The nanotube surface shows better wettability and higher surface energy compared to the bare substrate. The nanotube surface exhibits a wide passivation range and good corrosion resistance. The growth of the nanotube array is the result of the combined action of the anodization and field-assisted dissolution. The nanotube array by two-step oxidation becomes more regular and orderly. Moreover, the nanotube array surface is non-toxic and favourable to cell adhesion and proliferation. Such nanotube array films are expected to have significant biomedical applications.

Laponite-based Nanomaterials for Biomedical Applications: A Review

2019 ◽  
Vol 25 (4) ◽  
pp. 424-443 ◽  
Author(s):  
Sabya S. Das ◽  
Neelam ◽  
Kashif Hussain ◽  
Sima Singh ◽  
Afzal Hussain ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Cell Adhesion ◽  
Targeted Drug Delivery ◽  
Biomedical Applications ◽  
Biodegradable Materials ◽  
Clay Material ◽  
Research Directions ◽  
Targeted Drug ◽  
Cell Adhesion And Proliferation

Laponite based nanomaterials (LBNMs) are highly diverse regarding their mechanical, chemical, and structural properties, coupled with shape, size, mass, biodegradability and biocompatibility. These ubiquitous properties of LBNMs make them appropriate materials for extensive applications. These have enormous potential for effective and targeted drug delivery comprised of numerous biodegradable materials which results in enhanced bioavailability. Moreover, the clay material has been explored in tissue engineering and bioimaging for the diagnosis and treatment of various diseases. The material has been profoundly explored for minimized toxicity of nanomedicines. The present review compiled relevant and informative data to focus on the interactions of laponite nanoparticles and application in drug delivery, tissue engineering, imaging, cell adhesion and proliferation, and in biosensors. Eventually, concise conclusions are drawn concerning biomedical applications and identification of new promising research directions.

scholarly journals Polysaccharide-Based Aerogel Production for Biomedical Applications: A Comparative Review

Materials ◽  
2021 ◽  
Vol 14 (7) ◽  
pp. 1631
Author(s):  
Mariangela Guastaferro ◽  
Ernesto Reverchon ◽  
Lucia Baldino
Keyword(s):  
Tissue Regeneration ◽  
Supercritical Co2 ◽  
Biomedical Applications ◽  
Freeze Drying ◽  
Time Interval ◽  
Comparative Review ◽  
Biomedical Field ◽  
Low Cytotoxicity ◽  
Gel Preparation ◽  
Cell Adhesion And Proliferation

A comparative analysis concerning bio-based gels production, to be used for tissue regeneration, has been performed in this review. These gels are generally applied as scaffolds in the biomedical field, thanks to their morphology, low cytotoxicity, and high biocompatibility. Focusing on the time interval 2015–2020, the production of 3D scaffolds of alginate, chitosan and agarose, for skin and bone regeneration, has mainly been investigated. Traditional techniques are critically reviewed to understand their limitations and how supercritical CO2-assisted processes could overcome these drawbacks. In particular, even if freeze-drying represents the most widespread drying technique used to produce polysaccharide-based cryogels, supercritical CO2-assisted drying effectively allows preservation of the nanoporous aerogel structure and removes the organic solvent used for gel preparation. These characteristics are essential for cell adhesion and proliferation.

scholarly journals GAKTpore: Stereological Characterisation Methods for Porous Foams in Biomedical Applications

Materials ◽  
2021 ◽  
Vol 14 (5) ◽  
pp. 1269
Author(s):  
Gareth Sheppard ◽  
Karl Tassenberg ◽  
Bogdan Nenchev ◽  
Joel Strickland ◽  
Ramy Mesalam ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Biomedical Applications ◽  
Large Scale ◽  
Collagen Scaffold ◽  
Porous Structures ◽  
Tissue Engineering Scaffolds ◽  
Biological Responses ◽  
Sem Micrograph ◽  
Cell Adhesion And Proliferation ◽  
Characterisation Methods

In tissue engineering, scaffolds are a key component that possess a highly elaborate pore structure. Careful characterisation of such porous structures enables the prediction of a variety of large-scale biological responses. In this work, a rapid, efficient, and accurate methodology for 2D bulk porous structure analysis is proposed. The algorithm, “GAKTpore”, creates a morphology map allowing quantification and visualisation of spatial feature variation. The software achieves 99.6% and 99.1% mean accuracy for pore diameter and shape factor identification, respectively. There are two main algorithm novelties within this work: (1) feature-dependant homogeneity map; (2) a new waviness function providing insights into the convexity/concavity of pores, important for understanding the influence on cell adhesion and proliferation. The algorithm is applied to foam structures, providing a full characterisation of a 10 mm diameter SEM micrograph (14,784 × 14,915 px) with 190,249 pores in ~9 min and has elucidated new insights into collagen scaffold formation by relating microstructural formation to the bulk formation environment. This novel porosity characterisation algorithm demonstrates its versatility, where accuracy, repeatability, and time are paramount. Thus, GAKTpore offers enormous potential to optimise and enhance scaffolds within tissue engineering.

Silk-based hybrid microfibrous mats as guided bone regeneration membranes

2021 ◽  
Author(s):  
Mi Wu ◽  
Zhengyi Han ◽  
Wen Liu ◽  
Jinrong Yao ◽  
Bingjiao Zhao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Adhesion ◽  
Bone Regeneration ◽  
Silk Fibroin ◽  
Guided Bone Regeneration ◽  
Regenerated Silk Fibroin ◽  
Marrow Mesenchymal Stem Cells ◽  
Cell Adhesion And Proliferation

LAPONITE® (LAP) nanoplatelets were incorporated within a regenerated silk fibroin (RSF) microfibrous mat via electrospinning, which exhibited better cell adhesion and proliferation of bone marrow mesenchymal stem cells (BMSCs) than the pristine RSF ones.

Effect of Surface Potential on NIH3T3 Cell Adhesion and Proliferation

2014 ◽  
Vol 118 (26) ◽  
pp. 14464-14470 ◽  
Author(s):  
Hsun-Yun Chang ◽  
Chih-Chieh Huang ◽  
Kang-Yi Lin ◽  
Wei-Lun Kao ◽  
Hua-Yang Liao ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Surface Potential ◽  
Nih3t3 Cell ◽  
Cell Adhesion And Proliferation ◽  
Effect Of Surface

Oligonucleotides carrying nucleoside antimetabolites as potential prodrugs

2021 ◽  
Vol 28 ◽  
Author(s):  
Carme Fàbrega ◽  
Anna Clua ◽  
Ramon Eritja ◽  
Anna Aviñó
Keyword(s):  
Antisense Oligonucleotides ◽  
Biomedical Applications ◽  
Enzymatic Degradation ◽  
Research Effort ◽  
Synergetic Effect ◽  
Chemotherapeutic Agents ◽  
Dna Nanostructures ◽  
Chemically Modified ◽  
Near Future ◽  
Large Research

Background: Nucleoside and nucleobase antimetabolites are an important class of chemotherapeutic agents for the treatment of cancer as well as other diseases. Introduction: In order to avoid undesirable side effects, several prodrug strategies have been developed for that purpose. In the present review, we describe a relatively unknown strategy that consists in the use of oligonucleotides modified with nucleoside antimetabolites as prodrugs. Method: The active nucleotides are generated by enzymatic degradation once incorporated into cells. This strategy has attracted large interest and is very active at present due to the continuous developments made on therapeutic oligonucleotides and the recent advances in the field of nanomaterials and nanomedicine. Results: A large research effort was done mainly in the improvement of the antiproliferative properties of nucleoside homopolymers, but recently, chemically modified aptamers, antisense oligonucleotides and/or siRNA carrying antiproliferative nucleotides have demonstrated a great potential due to the synergetic effect of both therapeutic entities. In addition, DNA nanostructures with interesting properties have been built to combine antimetabolites and enhancers of cellular uptake in the same scaffold. Finally, protein nanoparticles functionalized with receptor-binders and antiproliferative oligomers represent a new avenue for a more effective treatment in cancer therapy. Conclusion: It is expected that oligonucleotides carrying nucleoside antimetabolites will be considered as potential drugs in the near future for biomedical applications.

scholarly journals Surface functionalisation of nanodiamonds for human neural stem cell adhesion and proliferation

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Alice C. Taylor ◽  
Citlali Helenes González ◽  
Benjamin S. Miller ◽  
Robert J. Edgington ◽  
Patrizia Ferretti ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Adhesion ◽  
Neural Stem Cell ◽  
Human Neural Stem Cell ◽  
Surface Functionalisation ◽  
Cell Adhesion And Proliferation

scholarly journals Three-Dimensional Bone Substitutes for Oral and Maxillofacial Surgery: Biological and Structural Characterization

2018 ◽  
Vol 9 (4) ◽  
pp. 62 ◽  
Author(s):  
Gianluca Turco ◽  
Davide Porrelli ◽  
Eleonora Marsich ◽  
Federica Vecchies ◽  
Teresa Lombardi ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Clinical Performance ◽  
Maxillofacial Surgery ◽  
Chemical Characterization ◽  
Bone Substitutes ◽  
Human Cell Line ◽  
Structural Features ◽  
Oral And Maxillofacial Surgery ◽  
Cell Adhesion And Proliferation

Background: Bone substitutes, either from human (autografts and allografts) or animal (xenografts) sources, suffer from inherent drawbacks including limited availability or potential infectivity to name a few. In the last decade, synthetic biomaterials have emerged as a valid alternative for biomedical applications in the field of orthopedic and maxillofacial surgery. In particular, phosphate-based bone substitution materials have exhibited a high biocompatibility due to their chemical similitude with natural hydroxyapatite. Besides the nature of the biomaterial, its porous and interconnected architecture is essential for a correct osseointegration. This performance could be predicted with an extensive characterization of the biomaterial in vitro. Methods: In this study, we compared the biological, chemical, and structural features of four different commercially available bone substitutes derived from an animal or a synthetic source. To this end, µ-CT and SEM were used to describe the biomaterials structure. Both FTIR and EDS analyses were carried out to provide a chemical characterization. The results obtained by these techniques were correlated with cell adhesion and proliferation of the osteosarcoma MG-63 human cell line cultured in vitro. Results: The findings reported in this paper indicate a significant influence of both the nature and the structure of the biomaterials in cell adhesion and proliferation, which ultimately could affect the clinical performance of the biomaterials. Conclusions: The four commercially available bone substitutes investigated in this work significantly differed in terms of structural features, which ultimately influenced in vitro cell proliferation and may so affect the clinical performance of the biomaterials.

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