scholarly journals A Preliminary Evaluation of the Pro-Chondrogenic Potential of 3D-Bioprinted Poly(ester Urea) Scaffolds

Polymers ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 1478 ◽  
Author(s):  
Samuel R. Moxon ◽  
Miguel J.S. Ferreira ◽  
Patricia dos Santos ◽  
Bogdan Popa ◽  
Antonio Gloria ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
In Vitro Model ◽  
Type Ii Collagen ◽  
Substrate Stiffness ◽  
Preliminary Evaluation ◽  
Chondrogenic Potential ◽  
Chondrogenic Phenotype ◽  
Vitro Model ◽  
Cell Phenotypes

Degeneration of articular cartilage (AC) is a common healthcare issue that can result in significantly impaired function and mobility for affected patients. The avascular nature of the tissue strongly burdens its regenerative capacity contributing to the development of more serious conditions such as osteoarthritis. Recent advances in bioprinting have prompted the development of alternative tissue engineering therapies for the generation of AC. Particular interest has been dedicated to scaffold-based strategies where 3D substrates are used to guide cellular function and tissue ingrowth. Despite its extensive use in bioprinting, the application of polycaprolactone (PCL) in AC is, however, restricted by properties that inhibit pro-chondrogenic cell phenotypes. This study proposes the use of a new bioprintable poly(ester urea) (PEU) material as an alternative to PCL for the generation of an in vitro model of early chondrogenesis. The polymer was successfully printed into 3D constructs displaying adequate substrate stiffness and increased hydrophilicity compared to PCL. Human chondrocytes cultured on the scaffolds exhibited higher cell viability and improved chondrogenic phenotype with upregulation of genes associated with type II collagen and aggrecan synthesis. Bioprinted PEU scaffolds could, therefore, provide a potential platform for the fabrication of bespoke, pro-chondrogenic tissue engineering constructs.

Use of an in vitro model in tissue engineering to study wound repair and differentiation of blastema tissue from rabbit pinna

2015 ◽  
Vol 51 (7) ◽  
pp. 680-689 ◽  
Author(s):  
Mohammad Reza Hashemzadeh ◽  
Nasser Mahdavi-Shahri ◽  
Ahmad Reza Bahrami ◽  
Masoumeh Kheirabadi ◽  
Fatemeh Naseri ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Repair ◽  
In Vitro Model ◽  
Vitro Model

Optimization of Schwann Cell Adhesion in Response to Shear Stress in an in Vitro Model for Peripheral Nerve Tissue Engineering

2003 ◽  
Vol 9 (2) ◽  
pp. 233-241 ◽  
Author(s):  
Dara Chafik ◽  
David Bear ◽  
Phong Bui ◽  
Arush Patel ◽  
Neil F. Jones ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Shear Stress ◽  
Cell Adhesion ◽  
Schwann Cell ◽  
Peripheral Nerve ◽  
In Vitro Model ◽  
Nerve Tissue ◽  
Nerve Tissue Engineering ◽  
Vitro Model

Computational Modeling of Cell Orientation in 3D Micro-Constructs

2013 ◽  
Author(s):  
Christine Obbink-Huizer ◽  
Cees W. J. Oomens ◽  
Sandra Loerakker ◽  
Jasper Foolen ◽  
Carlijn V. C. Bouten ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Computational Modeling ◽  
Computational Model ◽  
In Vitro Model ◽  
Stress Fibers ◽  
Cell Behavior ◽  
Cell Orientation ◽  
Mechanical Environment ◽  
Vitro Model

In many tissue engineering applications it is essential to understand how cells orient under the influence of their mechanical environment. In vitro engineered models are used to investigate the orientation of F-actin stress fibers inside cells. One such in vitro model [1] consists of a mixture of cells, collagen and matrigel, that is constrained by an array of silicone posts (Figure 1). We have recently developed a computational model to describe the orientation of stress fibers in response to their mechanical environment [2]. In the present study, this computational model is extended to 3D and used to simulate cell behavior in the mentioned in vitro model. This improves our understanding of how stress fibers orient in response to the mechanical environment and aids in optimizing the use of the in vitro model.

scholarly journals Tissue engineering in an in vitro model of human cartilage repair

2016 ◽  
Vol 24 ◽  
pp. S169-S170 ◽  
Author(s):  
C. Sanjurjo Rodriguez ◽  
R. Castro Viñuelas ◽  
T. Hermida Gomez ◽  
I. Fuentes Boquete ◽  
F. De Toro Santos ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Repair ◽  
In Vitro Model ◽  
Human Cartilage ◽  
Vitro Model

scholarly journals Efficacy of collagen and alginate hydrogels for the prevention of rat chondrocyte dedifferentiation

2018 ◽  
Vol 9 ◽  
pp. 204173141880243 ◽  
Author(s):  
Guang-Zhen Jin ◽  
Hae-Won Kim
Keyword(s):  
Tissue Engineering ◽  
Type I Collagen ◽  
Cartilage Tissue Engineering ◽  
Collagen Gel ◽  
Type Ii Collagen ◽  
Cartilage Tissue ◽  
Type I ◽  
Type Ii ◽  
Chondrogenic Phenotype

Dedifferentiation of chondrocytes remains a major problem in cartilage tissue engineering. The development of hydrogels that can preserve chondrogenic phenotype and prevent chondrocyte dedifferentiation is a meaningful strategy to solve dedifferentiation problem of chondrocytes. In the present study, three gels were prepared (alginate gel (Alg gel), type I collagen gel (Col gel), and their combination gel (Alg/Col gel)), and the in vitro efficacy of chondrocytes culture while preserving their phenotypes was investigated. While Col gel became substantially contracted with time, the cells encapsulated in Alg gel preserved the shape over the culture period of 14 days. The mechanical and cell-associated contraction behaviors of Alg/Col gel were similar to those of Alg. The cells in Alg and Alg/Col gels exhibited round morphology, whereas those in Col gel became elongated (i.e. fibroblast-like) during cultures. The cells proliferated with time in all gels with the highest proliferation being attained in Col gel. The expression of chondrogenic genes, including SOX9, type II collagen, and aggrecan, was significantly up-regulated in Alg/Col gel and Col gel, particularly in Col gel. However, the chondrocyte dedifferentiation markers, type I collagen and alkaline phosphatase ( ALP), were also expressed at significant levels in Col gel, which being contrasted with the events in Alg and Alg/Col gels. The current results suggest the cells cultured in hydrogels can express chondrocyte dedifferentiation markers as well as chondrocyte markers, which draws attention to choose proper hydrogels for chondrocyte-based cartilage tissue engineering.

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