scholarly journals Fabrication of Injectable, Porous Hyaluronic Acid Hydrogel Based on an In-Situ Bubble-Forming Hydrogel Entrapment Process

Polymers ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1138
Author(s):  
Lixuan Wang ◽  
Shiyan Dong ◽  
Yutong Liu ◽  
Yifan Ma ◽  
Jingjing Zhang ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Regenerative Medicine ◽  
In Vivo Studies ◽  
Injectable Hydrogels ◽  
Cell Behaviors ◽  
Porous Architecture ◽  
In Situ Hydrogel

Injectable hydrogels have been widely applied in the field of regenerative medicine. However, current techniques for injectable hydrogels are facing a challenge when trying to generate a biomimetic, porous architecture that is well-acknowledged to facilitate cell behaviors. In this study, an injectable, interconnected, porous hyaluronic acid (HA) hydrogel based on an in-situ bubble self-generation and entrapment process was developed. Through an amide reaction between HA and cystamine dihydrochloride activated by EDC/NHS, CO2 bubbles were generated and were subsequently entrapped inside the substrate due to a rapid gelation-induced retention effect. HA hydrogels with different molecular weights and concentrations were prepared and the effects of the hydrogel precursor solution’s concentration and viscosity on the properties of hydrogels were investigated. The results showed that HA10-10 (10 wt.%, MW 100,000 Da) and HA20-2.5 (2.5 wt.%, MW 200,000 Da) exhibited desirable gelation and obvious porous structure. Moreover, HA10-10 represented a high elastic modulus (32 kPa). According to the further in vitro and in vivo studies, all the hydrogels prepared in this study show favorable biocompatibility for desirable cell behaviors and mild host response. Overall, such an in-situ hydrogel with a self-forming bubble and entrapment strategy is believed to provide a robust and versatile platform to engineer injectable hydrogels for a variety of applications in tissue engineering, regenerative medicine, and personalized therapeutics.

Comparison of the intestinal absorption and bioavailability of γ-tocotrienol and α-tocopherol: in vitro, in situ and in vivo studies

2012 ◽  
Vol 33 (5) ◽  
pp. 246-256 ◽  
Author(s):  
Bilal S. Abuasal ◽  
Hisham Qosa ◽  
Paul W. Sylvester ◽  
Amal Kaddoumi
Keyword(s):  
Intestinal Absorption ◽  
In Vivo Studies

Mitochondrial Targeted Doxorubicin-Triphenylphosphonium Delivered by Hyaluronic Acid Modified and pH Responsive Nanocarriers to Breast Tumor: in Vitro and in Vivo Studies

2018 ◽  
Vol 15 (3) ◽  
pp. 882-891 ◽  
Author(s):  
Hui-Na Liu ◽  
Ning-Ning Guo ◽  
Tian-Tian Wang ◽  
Wang-Wei Guo ◽  
Meng-Ting Lin ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Breast Tumor ◽  
In Vivo Studies ◽  
Ph Responsive

scholarly journals Targetability of hyaluronic acid nanogel to cancer cells : In vitro and in vivo studies

2017 ◽  
Vol 104 ◽  
pp. 102-113 ◽  
Author(s):  
S.S. Pedrosa ◽  
P. Pereira ◽  
A. Correia ◽  
F.M. Gama
Keyword(s):  
Hyaluronic Acid ◽  
Cancer Cells ◽  
In Vivo Studies

Wound healing of nanofiber comprising Polygalacturonic/Hyaluronic acid embedded silver nanoparticles: In-vitro and in-vivo studies

2020 ◽  
Vol 238 ◽  
pp. 116175 ◽  
Author(s):  
M.R. El-Aassar ◽  
Omar M. Ibrahim ◽  
Moustafa M.G. Fouda ◽  
Nagham G. El-Beheri ◽  
Mona M. Agwa
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
Hyaluronic Acid ◽  
In Vivo Studies

scholarly journals A biomimetically hierarchical polyetherketoneketone scaffold for osteoporotic bone repair

2020 ◽  
Vol 6 (50) ◽  
pp. eabc4704
Author(s):  
Bo Yuan ◽  
Linnan Wang ◽  
Rui Zhao ◽  
Xi Yang ◽  
Xiao Yang ◽  
...  
Keyword(s):  
Bone Repair ◽  
Osteoporotic Fractures ◽  
In Vivo Studies ◽  
Osteoporotic Bone ◽  
Worldwide Population ◽  
Repair Materials ◽  
Porous Architecture ◽  
Viable Approach

Osteoporotic fractures are prevalent in society, and their incidence appears to be increasing as the worldwide population ages. However, conventional bone repair materials hardly satisfy the requirements for the repair of pathological fractures. Here, we developed a biomimetic polyetherketoneketone scaffold with a functionalized strontium-doped nanohydroxyapatite coating for osteoporotic bone defect applications. The scaffold has a hierarchically porous architecture and mechanical strength similar to that of osteoporotic trabecular bone. In vitro and in vivo studies demonstrated that the scaffold could promote osteoporotic bone regeneration and delay adjacent bone loss via regulating both osteoblasts and osteoclasts. In addition, the correlations between multiple preimplantation and postimplantation parameters were evaluated to determine the potential predictors of in vivo performance of the material. The current work not only develops a promising candidate for osteoporotic bone repair but also provides a viable approach for designing other functional biomaterials and predicting their translational value.

scholarly journals In Situ Cross-linking Hydrogel as a Vehicle for Retinal Progenitor Cell Transplantation

2019 ◽  
Vol 28 (5) ◽  
pp. 596-606 ◽  
Author(s):  
Jeayoung Park ◽  
Petr Baranov ◽  
Aybike Aydin ◽  
Hany Abdelgawad ◽  
Deepti Singh ◽  
...  
Keyword(s):  
In Vivo Studies ◽  
Subretinal Space ◽  
Cross Linking ◽  
Post Transplantation ◽  
Ki 67 ◽  
Retinal Progenitor ◽  
Stem Cell Delivery

One of the current limitations of retinal transplantation of stem cells as well as other cell types is the dispersion of cells from the injection site (including loss of cells into the vitreous chamber) and low survival after transplantation. Gelatin-hydroxyphenyl propionic acid (Gtn-HPA) conjugate is a biodegradable polymer that can undergo covalent cross-linking in situ, allowing for injection of incorporated cells through a small caliber needle followed by gel formation in vivo. We tested the hypothesis that Gtn-HPA hydrogel supports survival and integration of retinal progenitor cells (RPCs) post-transplantation. In vitro compatibility and in vivo graft survival were assessed by mixing an equal volume of Gtn-HPA conjugate and RPC suspension and triggering enzyme-mediated gelation, using minute amounts of horseradish peroxidase and peroxide. Immunocytochemistry showed >80% survival of cells and minimal apoptosis for cells incorporated into Gtn-HPA, equivalent to controls grown on fibronectin-coated flasks. RPCs undergoing mitosis were seen within the three-dimensional Gtn-HPA hydrogel, but the percentage of Ki-67-positive cells was lower compared with the monolayer controls. For in vivo studies, gel–cell mixture or cell suspension in saline was trans-sclerally injected into the left eye of female Long Evans rats immunosuppressed with cyclosporine A. Grafts survived at the 1 week time point of the study, with Gtn-HPA-delivered grafts showing less inflammatory response demonstrated by anti-leukocyte staining. More eyes in the gel–cell mixture group showed surviving cells in the subretinal space compared with saline-delivered controls, while the number of cells surviving per graft was not significantly different between the two groups. This work demonstrates an injectable in situ cross-linking hydrogel as a potential vehicle for stem cell delivery in the retina.

scholarly journals Cross-linked hyaluronic acid gel inhibits metastasis and growth of gastric and hepatic cancer cells: in vitro and in vivo studies

Oncotarget ◽  
2016 ◽  
Vol 7 (40) ◽  
pp. 65418-65428 ◽  
Author(s):  
Ting Lan ◽  
Ji Pang ◽  
Yan Wu ◽  
Miaolin Zhu ◽  
Xiaoyuan Yao ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Cancer Cells ◽  
In Vivo Studies ◽  
Hepatic Cancer ◽  
Acid Gel ◽  
Hyaluronic Acid Gel

Preparation and characterization of antibacterial and anti-inflammatory hyaluronic acid-chitosan-dexamethasone hydrogels for peri-implantitis repair

2021 ◽  
pp. 088532822110479
Author(s):  
Zhen Zhou ◽  
Qiang Zhang ◽  
Yamin Wang
Keyword(s):  
Hyaluronic Acid ◽  
Physicochemical Characterization ◽  
Inflammatory Drug ◽  
Composite Hydrogels ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Nih 3T3

Numerous treatment methods for peri-implantitis have been widely used including oral cleaning, traditional metal scraping means, or local antibiotic application. However, to continuously release antibacterial and anti-inflammatory drug in location in situ for effective peri-implantitis repair is still challenging. Herein, an anti-inflammatory drug dexamethasone (DE)–incorporated hyaluronic acid (HA)-chitosan (CT) composite hydrogels system was developed to repair peri-implantitis. The physicochemical characterization and biocompatibility of the hydrogel were evaluated in vitro. The in vivo hydrogels degradation and peri-implantitis repair were assessed in mice. The results showed that the prepared multifunctional hydrogels achieved sustained release, with an equilibrium swelling of 18, and promoted the growth against NIH-3T3 fibroblast cells. The in vitro antibacterial tests showed HA-CT-DE hydrogels can inhibit methicillin-resistant Staphylococcus aureus and Escherichia coli. It down-regulated the expression levels of inflammation factor IL-1β, IL-6 and, TNF-α in peri-implantitis. The prepared HA-CT-DE composite hydrogels with integrated function is promising for the treatment of peri-implantitis.

scholarly journals FORMULATION AND EVALUATION OF NOVEL IN SITU GEL OF LAFUTIDINE FOR GASTRO RETENTIVE DRUG DELIVERY

2018 ◽  
Vol 11 (8) ◽  
pp. 88
Author(s):  
Sindhoor S M ◽  
Sneh Priya ◽  
Amala Maxwell
Keyword(s):  
Drug Release ◽  
Imaging Study ◽  
Lag Time ◽  
In Vivo Studies ◽  
In Vitro Drug Release ◽  
In Situ Gel ◽  
Drug Content

Objective: The aim of the present study was to formulate and evaluate the novel in situ gel of lafutidine for gastroretentive drug deliveryMethods: A gastroretentive in situ gel of lafutidine was formulated by pH-triggered ionic gelation method using different concentrations of gelling polymer such as sodium alginate, gellan gum, and xanthum gum. Prepared formulations were evaluated for viscosity, density, buoyancy lag time and buoyancy duration, and drug content. In vitro drug release studies of all formulations were also performed. In vivo fluorescence imaging study was conducted for optimized formulation and compared with control.Results: The concentration of gelling agents and release retardant polymers significantly affected viscosity, floating behavior, and in vitro drug release of the formulations. The pH and drug content were found in the range of 6.72–7.20 and 88.74–95.33%, respectively. Floating lag time was <2 min; duration of floating was more than 12 h. Minimum and maximum in vitro drug release were found to be for formulation F9 (51.74%) and F1 (82.76%), respectively, at the end of 12 h. The drug was released from the all the formulations in a sustained manner. In vivo studies confirmed the gastroretention of the formulation in mice stomach for 8 h. Stability studies indicated that the there was no significant change in the visual appearance, floating behavior, and drug content.Conclusion: The gastroretentive in situ gel system, prolonged the gastric residence time, thereby targeting site-specific drug release in the upper gastrointestinal tract.

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