scholarly journals Graphene Oxide Composite for Selective Recognition, Capturing, Photothermal Killing of Bacteria over Mammalian Cells

Polymers ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1116 ◽  
Author(s):  
Gang Ma ◽  
Junjie Qi ◽  
Qifan Cui ◽  
Xueying Bao ◽  
Dong Gao ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Mammalian Cells ◽  
Selective Recognition ◽  
Great Promise ◽  
Poly Ethylene Glycol ◽  
Amino Groups ◽  
Low Cytotoxicity ◽  
Poly Ethylene ◽  
Insight Into ◽  
Positively Charged

The multifunctional photothermal therapy (PTT) platform with the ability to selectively kill bacteria over mammalian cells has received widespread attention recently. Herein, we prepared graphene oxide-amino(polyethyleneglycol) (GO-PEG-NH2) while using the hydrophobic interaction between heptadecyl end groups of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethyleneglycol)] (DSPE-PEG-NH2) and graphene oxide (GO). Based on GO-PEG-NH2, the versatile PTT system was constructed with simultaneous selective recognition, capturing, and photothermal killing of bacteria. When the cells undergo bacterial infection, owing to the poly(ethylene glycol) (PEG) chains and positively charged amino groups, GO-PEG-NH2 can specifically recognize and capture bacteria in the presence of cells. Meanwhile, the stable photothermal performance of GO-PEG-NH2 enables the captured bacteria to be efficiently photothermally ablated upon the irradiation of 808 nm laser. Besides, the GO-PEG-NH2 is highly stable in various biological media and it exhibits low cytotoxicity, suggesting that it holds great promise for biological applications. This work provides new insight into graphene-based materials as a PTT agent for the development of new therapeutic platforms.

A stir foam composed of graphene oxide, poly(ethylene glycol) and natural latex for the extraction of preservatives and antioxidant

2018 ◽  
Vol 185 (2) ◽  
Author(s):  
Charinrat Siritham ◽  
Chongdee Thammakhet-Buranachai ◽  
Panote Thavarungkul ◽  
Proespichaya Kanatharana
Keyword(s):  
Graphene Oxide ◽  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

scholarly journals Haemolytic activity of stonustoxin from stonefish (Synanceja horrida) venom: pore formation and the role of cationic amino acid residues

1997 ◽  
Vol 325 (3) ◽  
pp. 685-691 ◽  
Author(s):  
Desong CHEN ◽  
R. Manjunatha KINI ◽  
Raymond YUEN ◽  
Hoon Eng KHOO
Keyword(s):  
Cell Membrane ◽  
Ethylene Glycol ◽  
Haemolytic Activity ◽  
Side Chains ◽  
Poly Ethylene Glycol ◽  
Arginine Residues ◽  
Poly Ethylene ◽  
Cationic Residues ◽  
Positively Charged

Stonustoxin (SNTX) is a two-subunit protein toxin purified from the venom of the stonefish (Synanceja horrida), which induces potent haemolytic activity. We examined the pore-forming property of this non-enzymic protein by an osmotic protection assay. SNTX-induced haemolysis was completely prevented by osmotic protectants of adequate size [poly(ethylene) glycol 3000; molecular diameter approx. 3.2 nm]. Uncharged molecules of smaller size, such as raffinose and poly(ethylene) glycol 1000–2000, failed to protect against cell lysis. These findings indicate that SNTX induces the formation of hydrophilic pores in the cell membrane, which results in the lysis of erythrocytes. Since cationic residues contribute significantly to the cytolytic activity of several other pore-forming toxins, we examined the role of positively charged lysine and arginine residues in the haemolytic activity of SNTX. SNTX lost its haemolytic activity when the positively charged side chains of lysine residues were neutralized or converted into negatively charged side chains upon carbamylation or succinylation respectively. The haemolytic activity of SNTX was also inhibited by the modification of positively charged arginine residues using 2,3-butanedione. The loss of haemolysis showed strong correlation with the number of Lys or Arg residues modified. CD analyses, however, showed that the conformation of SNTX was not significantly affected by these chemical modifications. Further, the haemolytic activity of SNTX was competitively inhibited by various negatively charged lipids, such as phosphatidylserine, cardiolipin and monosialogangliosides. These results indicate that SNTX induces potent haemolytic activity through the formation of pores in the cell membrane, and that cationic residues play a crucial role in its cytolytic mechanism.

scholarly journals Comparison of triblock copolymeric micelles based on α- and ε-poly(L-lysine): a Cornelian choice

2021 ◽  
Author(s):  
Franck Marquet ◽  
Viorica Patrulea ◽  
Gerrit Borchard
Keyword(s):  
Triblock Copolymer ◽  
Colloidal Stability ◽  
Solid Phase ◽  
Clinical Applications ◽  
Poly Ethylene Glycol ◽  
Amino Groups ◽  
Molecular Weights ◽  
Hydrophobic Compounds ◽  
Poly Ethylene ◽  
Interfering Rna

AbstractDue to the lack of safe carriers for the delivery of small interfering RNA (siRNA), clinical applications of nucleotide-based therapeutics have been limited. In this study, biodegradable amphiphilic triblock copolymers with tailored molecular weights for each block composed of methoxy poly(ethylene glycol) (2000 g/mol), poly(L-lysine) (1300 g/mol) and poly(D,L-lactic acid) (1800 g/mol) (mPEG45-α-PLL10-PLA25) were synthesized and fully characterized. The peptide synthesis was carried out on a solid phase to limit the presence of cationic charges. The arrangement and availability of cationic amino groups within a micellar vector were investigated to determine the colloidal stability as well as the predisposition of these systems to vectorize siRNAs in addition to their already known ability to improve the solubility of hydrophobic compounds. For this purpose, a triblock copolymer containing an epsilon poly(L-lysine) was synthesized similarly. Accordingly, the arrangement of the cationic segment modifies the rigidity involving a complexation constraint due to limited cationic charges available on the surface, which can compromise the efficiency of delivery into cells. In addition, the two vectors were biocompatible in different human cell lines.

Novel nanocomposites of graphene oxide reinforced poly (3,4-ethylenedioxythiophene)-block-poly (ethylene glycol) and polyvinylidene fluoride for embedded capacitor applications

RSC Advances ◽  
2014 ◽  
Vol 4 (71) ◽  
pp. 37954-37963 ◽  
Author(s):  
Kalim Deshmukh ◽  
Girish M. Joshi
Keyword(s):  
Graphene Oxide ◽  
Ethylene Glycol ◽  
Polyvinylidene Fluoride ◽  
Poly Ethylene Glycol ◽  
Embedded Capacitor ◽  
Fabrication And Characterization ◽  
Poly Ethylene

The fabrication and characterization of nanocomposites consisting of graphene oxide (GO) reinforced poly(3,4-ethylenedioxythiophene)-block-poly (ethylene glycol) (PEDOT-block-PEG)/polyvinylidenefluoride (PVDF) were investigated.

Positively Charged Oligo[Poly(Ethylene Glycol) Fumarate] Scaffold Implantation Results in a Permissive Lesion Environment after Spinal Cord Injury in Rat

2015 ◽  
pp. 150617132638005 ◽  
Author(s):  
Jeffrey S. Hakim ◽  
Melika Esmaeili Rad ◽  
Peter J. Grahn ◽  
Bingkun K. Chen ◽  
Andrew M. Knight ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Cord Injury ◽  
Poly Ethylene ◽  
Positively Charged

A facile approach to biodegradable poly(ε-caprolactone)-poly(ethylene glycol)-based polyurethanes containing pendant amino groups

2007 ◽  
Vol 43 (5) ◽  
pp. 2080-2087 ◽  
Author(s):  
Zhigang Xie ◽  
Changhai Lu ◽  
Xuesi Chen ◽  
Li Chen ◽  
Xiuli Hu ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Amino Groups ◽  
Biodegradable Poly ◽  
Poly Ethylene
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