scholarly journals The Use of Lignin as a Microbial Carrier in the Co-Digestion of Cheese and Wafer Waste

Polymers ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 2073 ◽  
Author(s):  
Agnieszka A. Pilarska ◽  
Agnieszka Wolna-Maruwka ◽  
Krzysztof Pilarski ◽  
Damian Janczak ◽  
Krzysztof Przybył ◽  
...  
Keyword(s):  
Volatile Fatty Acids ◽  
Biogas Production ◽  
Cell Immobilization ◽  
Total Alkalinity ◽  
Batch Reactors ◽  
Adhesive Properties ◽  
Volatile Solids ◽  
Wide Ph Range ◽  
Ph Range ◽  
Microbiological Analyses

The aim of the article was to present the effects of lignin grafted with polyvinylpyrrolidone (PVP) as a microbial carrier in anaerobic co-digestion (AcoD) of cheese (CE) and wafer waste (WF). Individual samples of waste cheese and wafers were also tested. The PVP modifier was used to improve the adhesive properties of the carrier surface. Lignin is a natural biopolymer which exhibits all the properties of a good carrier, including nontoxicity, biocompatibility, porosity, and thermal stability. Moreover, the analysis of the zeta potential of lignin and lignin combined with PVP showed their high electrokinetic stability within a wide pH range, that is, 4–11. The AcoD process was conducted under mesophilic conditions in a laboratory by means of anaerobic batch reactors. Monitoring with two standard parameters: pH and the VFA/TA ratio (volatile fatty acids-to-total alkalinity ratio) proved that the process was stable in all the samples tested. The high share of N–NH4+ in TKN (total Kjeldahl nitrogen), which exceeded 90% for WF+CE and CE at the last phases of the process, proved the effective conversion of nitrogen forms. The microbiological analyses showed that eubacteria proliferated intensively and the dehydrogenase activity increased in the samples containing the carrier, especially in the system with two co-substrates (WF+CE/lignin) and in the waste cheese sample (CE/lignin). The biogas production increased from 1102.00 m3 Mg−1 VS (volatile solids) to 1257.38 m3 Mg−1 VS in the WF+CE/lignin sample, and from 881.26 m3 Mg−1 VS to 989.65 m3 Mg−1 VS in the CE/lignin sample. The research results showed that the cell immobilization on lignin had very positive effect on the anaerobic digestion process.

Impact of volatile fatty acids to alkalinity ratio and volatile solids on biogas production under thermophilic conditions

2020 ◽  
pp. 0734242X2095739
Author(s):  
Abdul-Aziz Issah ◽  
Telesphore Kabera
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Biogas Production ◽  
Total Alkalinity ◽  
Stable Range ◽  
Start Up ◽  
Volatile Solids ◽  
Significant Difference ◽  
Thermophilic Conditions ◽  
The Impact

The study assessed the impact of volatile fatty acids (VFA) to total alkalinity (TA) ratio (VFA/TA), and percentage volatile solids (VS) reduction of batch and semi-continuous anaerobic co-digestion of palm nut paste waste (PNPW) and anaerobic-digested rumen waste (ADRW) on digester stability and biogas production under the environmental condition of 50 ± 1°C and hydraulic retention time of 21 days for the batch studies and 14 days for semi-continuous co-digestion. The co-digestion ratios were based on percentage digester volume corresponding to 90%:10%, 75%:25% and 50%:50%. During batch and semi-continuous anaerobic co-digestion, VFA/TA of 0.32–1.0 and VS reduction of 53–67% were observed as the stable range at which biogas production was maximum. In terms of semi-continuous anaerobic digestion (AD), except for the 50%:50% ratio where biogas production progressed steadily from the first to fourteenth days, biogas production initially dropped from 180.1 to 171.3 mL between the first and third days of the 90%:10% reaching a maximum of 184 mL on the fourteenth day. Biogas production declined from 198.8 to 187.5 mL on the second day and then increased to 198.8 ± 0.5 mL in the case of the 75%:25% with a significant difference between the treatment ratios at p < 0.05. Therefore, the study can confirm that the 50%:50% ratio (PNPW:ADRW) is a suitable option for managing crude fat-based waste under thermophilic AD due to its potential for rapid start-up and complete biodegradation of active biomass within a 21-day period. This presupposes that residual methane as greenhouse gas will be void in the effluent if disposed of.

Sludge electrooxidation as pre-treatment for anaerobic digestion

2016 ◽  
Vol 75 (4) ◽  
pp. 775-781 ◽  
Author(s):  
J. A. Barrios ◽  
U. Duran ◽  
A. Cano ◽  
M. Cisneros-Ortiz ◽  
S. Hernández
Keyword(s):  
Anaerobic Digestion ◽  
Volatile Fatty Acids ◽  
Biogas Production ◽  
Oxygen Demand ◽  
Wastewater Sludge ◽  
Boron Doped Diamond ◽  
Boron Doped ◽  
Treatment Conditions ◽  
Volatile Solids ◽  
Pre Treatment

Anaerobic digestion of wastewater sludge is the preferred method for sludge treatment as it produces energy in the form of biogas as well as a stabilised product that may be land applied. Different pre-treatments have been proposed to solubilise organic matter and increase biogas production. Sludge electrooxidation with boron-doped diamond electrodes was used as pre-treatment for waste activated sludge (WAS) and its effect on physicochemical properties and biomethane potential (BMP) was evaluated. WAS with 2 and 3% total solids (TS) achieved 2.1 and 2.8% solubilisation, respectively, with higher solids requiring more energy. After pre-treatment, biodegradable chemical oxygen demand values were close to the maximum theoretical BMP, which makes sludge suitable for energy production. Anaerobic digestion reduced volatile solids (VS) by more than 30% in pre-treated sludge with a food to microorganism ratio of 0.15 g VSfed g−1 VSbiomass. Volatile fatty acids were lower than those for sludge without pre-treatment. Best pre-treatment conditions were 3% TS and 28.6 mA cm−2.

scholarly journals Comparison between biodegradable polymers from cassava starch and glycerol as additives to biogas production

2016 ◽  
Vol 37 (4) ◽  
pp. 1827 ◽  
Author(s):  
Paulo André Cremonez ◽  
Armin Feiden ◽  
Joel Gustavo Teleken ◽  
Samuel Nelson Melegari de Souza ◽  
Michael Feroldi ◽  
...  
Keyword(s):  
Biodegradable Polymers ◽  
Biogas Production ◽  
Oxygen Demand ◽  
Cassava Starch ◽  
Swine Wastewater ◽  
Batch Reactors ◽  
Total Volatile ◽  
Swine Waste ◽  
Volatile Solids ◽  
Working Volume

In this study, we compared cassava starch-based biodegradable polymers (PBMs) and glycerol (G) as additives used to increase biogas production from the co-digestion of swine wastewater (ARS). We chose to work with an inoculum comprising 40% (v/v) of the total volume of the reactor; this inoculum was obtained from a Canadian model digester for treating swine waste. In the anaerobic digestion process, batch reactors were used on a laboratory scale with a total volume of approximately 4 L and a working volume of 3.2 L. Three treatments were conducted to compare the efficiency of solid removal, the chemical oxygen demand (COD), and the production of biogas. The first treatment contained only swine waste; the second included the addition of glycerol at 1, 3, and 5% (w/v); and the third treatment included the addition of 1, 3, and 5% (w/v) of PBM residue in relation to the swine wastewater. From the results, it can be concluded that higher yields were obtained for the treatment with 3% PBM and 1% glycerol. Most treatments showed high removal rates of total solids and total volatile solids. Reductions lower than 70% were obtained only for treatments with PBM and glycerol at a ratio of 5%.

Anaerobic co-digestion of spent coconut copra with cow urine for enhanced biogas production

2020 ◽  
pp. 0734242X2097509
Author(s):  
Uduak U Ndubuisi-Nnaji ◽  
Utibe A Ofon ◽  
Nnanake-Abasi O Offiong
Keyword(s):  
Performance Indicators ◽  
Volatile Fatty Acids ◽  
Control Experiment ◽  
Biogas Production ◽  
Batch Mode ◽  
Mixing Ratios ◽  
Volatile Solids ◽  
Cow Urine ◽  
Thermophilic Condition ◽  
Biomethane Production

Laboratory-scale bioreactors were used to co-digest spent coconut copra (SCC) and cow urine (CU) as a co-substrate (SCC + CU) in a batch mode under thermophilic condition (45 ± 2°C) in order to enhance biogas production. The effect of CU pretreatment on the performance indicators (biogas and biomethane yields, total solids (TS), and volatile solids (VS) reduction, pH and volatile fatty acids (VFAs) concentrations) were also examined. This was compared with mono-digestion of SCC. The experiment was performed with different mixing ratios in reactors labelled as follows: A = 75 g SCC + 5 ml CU; B = 70 g SCC + 10 ml CU; C = 65 g SCC + 15 ml CU; and D (control) = 80 g SCC at a hydraulic retention time of 42 days. Co-digestion (SCC + CU) significantly improved anaerobic digestion (AD) performance resulting in a threefold and fivefold increase in biogas and biomethane production, respectively, with concomitant TS (44.9–57.7%) and VS (55.4–60.3%) removal efficiencies. But for mono-digestion (control experiment), all CU treated and co-digestion assays showed pH stability ranging between 6.6 and 7.4 and VFAs’ concentrations ranging from 15–330 mgL-1. By acting as a buffer, CU effectively enhanced the AD performance of SCC as demonstrated in this study.

scholarly journals Anaerobic digestion of donkey dung for biogas production

2016 ◽  
Vol Volume 112 (Number 7/8) ◽  
Author(s):  
Patrick Mukumba ◽  
Golden Makaka ◽  
Sampson Mamphweli ◽  
◽  
◽  
...  
Keyword(s):  
Rural Areas ◽  
Biogas Production ◽  
Oxygen Demand ◽  
Calorific Value ◽  
Ammonium Nitrogen ◽  
Total Alkalinity ◽  
Eastern Cape ◽  
Energy Needs ◽  
Volatile Solids ◽  
The University

Abstract Biogas can provide a solution to some of South Africa’s energy needs, especially in rural areas of Eastern Cape Province that have plentiful biogas substrates from donkeys, goats, sheep, cattle and chicken. We investigated the effectiveness of donkey dung for biogas production using a designed and constructed cylindrical field batch biogas digester. The donkey dung was collected from the University of Fort Hare’s Honeydale Farm and was analysed for total solids, volatile solids, total alkalinity, calorific value, pH, chemical oxygen demand and ammonium nitrogen (NH4-N). The biogas composition was analysed using a gas analyser. We found that donkey dung produced biogas with an average methane yield of 55% without co-digesting it with other wastes. The results show that donkey dung is an effective substrate for biogas production.

scholarly journals Microbial and Phenyl Acid Dynamics during the Start-up Phase of Anaerobic Straw Degradation in Meso- and Thermophilic Batch Reactors

2019 ◽  
Vol 7 (12) ◽  
pp. 657 ◽  
Author(s):  
Eva Maria Prem ◽  
Rudolf Markt ◽  
Nina Lackner ◽  
Paul Illmer ◽  
Andreas Otto Wagner
Keyword(s):  
Anaerobic Degradation ◽  
Volatile Fatty Acids ◽  
Biogas Production ◽  
Lignocellulose Degradation ◽  
Batch Reactors ◽  
Highly Active ◽  
Start Up ◽  
Negative Effect ◽  
Complex Organic ◽  
Generation Sequencing

Aromatic compounds like phenyl acids derived from lignocellulose degradation have been suspected to negatively influence biogas production processes. However, results on this topic are still inconclusive. To study phenyl acid formation in batch reactors during the start-up phase of anaerobic degradation, different amounts of straw from grain were mixed with mesophilic and thermophilic sludge, respectively. Molecular biological parameters were assessed using next-generation sequencing and qPCR analyses. Metagenomic predictions were done via the program, piphillin. Methane production, concentrations of phenylacetate, phenylpropionate, phenylbutyrate, and volatile fatty acids were monitored chromatographically. Methanosarcina spp. was the dominant methanogen when high straw loads were effectively degraded, and thus confirmed its robustness towards overload conditions. Several microorganisms correlated negatively with phenyl acids; however, a negative effect, specifically on methanogens, could not be proven. A cascade-like increase/decrease from phenylacetate to phenylpropionate, and then to phenylbutyrate could be observed when methanogenesis was highly active. Due to these results, phenylacetate was shown to be an early sign for overload conditions, whereas an increase in phenylbutyrate possibly indicated a switch from degradation of easily available to more complex substrates. These dynamics during the start-up phase might be relevant for biogas plant operators using complex organic wastes for energy exploitation.

scholarly journals Efektivitas Pemanfaatan Serbuk Gergaji dan Limbah Media Tanam Jamur (Baglog) sebagai Bahan Baku Pembuatan Biogas

2016 ◽  
Vol 2 (1) ◽  
pp. 11-16 ◽  
Author(s):  
Dikdik Mulyadi ◽  
Lela Mukmilah Yuningsih ◽  
Desi Kusumawati
Keyword(s):  
Fermentation Process ◽  
Volatile Fatty Acids ◽  
Anaerobic Fermentation ◽  
Biogas Production ◽  
Volume Measurement ◽  
Raw Material ◽  
Cow Dung ◽  
Total Volatile ◽  
A Value ◽  
Volatile Solids

Biogas is  one of energy   that can be produced by anaerobic fermentation of the organic compounds. The objective of this study was to determine the effectiveness of the utilization of waste of media  mushroom growth (baglog) with sawdust as raw material for biogas with  cow dung  activators. The study was conducted through anaerobic fermentation of the samples containing waste baglog (sample 1) and sawdust (sample 2), with the addition of cow manure activator to each sample. Both of these samples do anaerobic fermentation for 32 days, then measuring the volume of biogas every 4 days for 32 days. Methane content  in  samples 1 and 2 measured by  using gas chromatography. To see the effect of the addition of activators cow dung biogas volume measurement was  carried out with  cow dung without addition baglog waste and sawdust. The process of degradation baglog and sawdust with an activator of cow dung could be observed  in  some of the parameters through  total solids (TS), total volatile solids (TVS), volatile fatty acids (VFA), the degree of acidity (pH), and C/N ratio. The results showed that effectiveness of sample 1 resulted in the everage of total volume biogas 28% higher than  sample 2. The content of methane in  sample 1  and sampel 2  was 54% %, and 0.21% respectively. The fermentation process biogas production in this experiment  was carried out  at pH 7, with a value of TS, TVS and VFA showed a decrease  trend after the fermentation process,  C/N ratiowas  lower than the baglog waste sawdust until day 32 retention time. Keywords: Sawdust, baglog waste, biogas, fermentation, methane DOI : http://dx.doi.org/10.15408/jkv.v2i1.3100

scholarly journals Kraft Lignin Grafted with Polyvinylpyrrolidone as a Novel Microbial Carrier in Biogas Production

Energies ◽  
2018 ◽  
Vol 11 (12) ◽  
pp. 3246 ◽  
Author(s):  
Agnieszka A. Pilarska ◽  
Agnieszka Wolna-Maruwka ◽  
Krzysztof Pilarski
Keyword(s):  
Anaerobic Digestion ◽  
Sewage Sludge ◽  
Volatile Fatty Acids ◽  
Biogas Production ◽  
Kraft Lignin ◽  
Physicochemical Analysis ◽  
Process Efficiency ◽  
Total Alkalinity ◽  
Elementary Analysis ◽  
Biochemical Analyses

The objective of this study was to verify the effect of kraft lignin as a microbial carrier on biogas/methane yield. An anaerobic co-digestion test process was carried out, in which confectionery waste was used with sewage sludge. At the first stage of the study pure lignin and lignin combined with polyvinylpyrrolidone (PVP) were subjected to an extensive physicochemical analysis. Their morphology, dispersion and adsorption properties were determined. The two materials were also subjected to thermal, spectroscopic and elementary analysis. The anaerobic digestion of the two substrates was carried out with and without the addition of the carrier, under mesophilic conditions and in periodic operation. The monitoring and analysis of the two essential parameters, i.e., pH and volatile fatty acids/total alkalinity (VFA/TA) ratio, revealed that the process was stable in both tests. Microbial and biochemical analyses showed intensified proliferation of eubacteria and increased dehydrogenase activity in samples prepared with the lignin + PVP material. The cell count increased by 46% in the stuffed wafers (WAF) + sewage sludge (SS) variant with the carrier, whereas the enzyme activity increased by 43%. Cell immobilisation noticeably improved the process efficiency. The biogas production increased from 722 m3 Mg−1 VS to 850 m3 Mg−1 VS (VS – volatile solids), whereas the methane production increased from 428 m3 Mg−1 VS to 503 m3 Mg−1 VS (by about 18%). The research proved that lignin could be used as a very effective microbial carrier in anaerobic digestion (AD).

Effects of disintegration on anaerobic degradation of sewage excess sludge in downflow stationary fixed film digesters

2000 ◽  
Vol 41 (3) ◽  
pp. 171-179 ◽  
Author(s):  
M. Engelhart ◽  
M. Krüger ◽  
J. Kopp ◽  
N. Dichtl
Keyword(s):  
Anaerobic Degradation ◽  
Volatile Fatty Acids ◽  
Biogas Production ◽  
Soluble Proteins ◽  
Excess Sludge ◽  
Mechanical Pretreatment ◽  
Volatile Solids ◽  
Fixed Film ◽  
High Pressure Homogenizer ◽  
Anaerobic Digestibility

The effects of mechanical disintegration on anaerobic digestibility of sewage excess sludge in downflow stationary fixed film (DSFF) digesters were investigated on laboratory scale. Mechanical pretreatment using a high pressure homogenizer led to significantly enhanced concentrations of soluble proteins and carbohydrates in the feed sludge. Using DSFF digesters with two different tubular plastic media as support material it was shown that a stable digestion process could be achieved at hydraulic retention times (HRT) down to 5 days. Compared to conventional digesters at 10 d and 15 d HRT respectively, the degradation of volatile solids was enhanced up to 25%, also resulting in a higher specific biogas production. Further investigations on degradation of soluble proteins and carbohydrates showed that a slowly degradable fraction of carbohydrates was released via disintegration. Using the distribution of chain length and the concentrations of volatile fatty acids as process parameters, the dependability on the HRT and the degree of disintegration (the release of soluble COD) predominated the effects of specific surface area of the support media.

scholarly journals Mesophilic and Thermophilic Anaerobic Digestion of Wheat Straw in a CSTR System with ‘Synthetic Manure’: Impact of Nickel and Tungsten on Methane Yields, Cell Count, and Microbiome

2022 ◽  
Vol 9 (1) ◽  
pp. 13
Author(s):  
Richard Arthur ◽  
Sebastian Antonczyk ◽  
Sandra Off ◽  
Paul A. Scherer
Keyword(s):  
Wheat Straw ◽  
Methane Production ◽  
Volatile Fatty Acids ◽  
Biogas Production ◽  
Cell Counts ◽  
Volatile Solids ◽  
Thermophilic Conditions ◽  
Pure Cultures ◽  
Biogas Reactors ◽  
And Tungsten

Lignocellulosic residues, such as straw, are currently considered as candidates for biogas production. Therefore, straw fermentations were performed to quantitatively estimate methane yields and cell counts, as well as to qualitatively determine the microbiome. Six fully automated, continuously stirred biogas reactors were used: three mesophilic (41 °C) and three thermophilic (58 °C). They were fed every 8 h with milled wheat straw suspension in a defined, buffered salt solution, called ‘synthetic manure’. Total reflection X-ray fluorescence spectrometry analyses showed nickel and tungsten deficiency in the straw suspension. Supplementation of nickel and subsequently tungsten, or with an increasing combined dosage of both elements, resulted in a final concentration of approximately 0.1 mg/L active, dissolved tungsten ions, which caused an increase of the specific methane production, up to 63% under mesophilic and 31% under thermophilic conditions. That is the same optimal range for pure cultures of methanogens or bacteria found in literature. A simultaneous decrease of volatile fatty acids occurred. The Ni/W effect occurred with all three organic loading rates, being 4.5, 7.5, and 9.0 g volatile solids per litre and day, with a concomitant hydraulic retention time of 18, 10, or 8 days, respectively. A maximum specific methane production of 0.254 m3 CH4, under standard temperature and pressure per kg volatile solids (almost 90% degradation), was obtained. After the final supplementation of tungsten, the cell counts of methanogens increased by 300%, while the total microbial cell counts increased by only 3–62%. The mesophilic methanogenic microflora was shifted from the acetotrophic Methanosaeta to the hydrogenotrophic Methanoculleus (85%) by tungsten, whereas the H2-CO2-converter, Methanothermobacter, always dominated in the thermophilic fermenters.

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