scholarly journals Wasted Ganoderma tsugae Derived Chitosans for Smear Layer Removal in Endodontic Treatment

Polymers ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 1795 ◽  
Author(s):  
Huang ◽  
Teng ◽  
Wang ◽  
Hsieh ◽  
Yang
Keyword(s):  
Citric Acid ◽  
Metal Ion ◽  
Synergistic Effects ◽  
Smear Layer ◽  
Ferrous Ions ◽  
Layer Removal ◽  
Significant Difference ◽  
Potential Alternative ◽  
Soluble Polysaccharide ◽  
Metal Ion Chelation

The objective of this study is to investigate the synergistic effects of acid etching and metal-ion chelation in dental smear layer removal using wasted Ganoderma tsugae derived chitosans. The wasted Ganoderma tsugae fruiting body was used to prepare both acid-soluble fungal chitosan (FCS) and alkali-soluble polysaccharide (ASP). To explore the effective irrigant concentration for smear layer removal, a chelating effect on ferrous ions was conducted. Specimens of various concentrations of EDTA, citric acid, and polysaccharide solutions were reacted with FerroZine™ then the absorbance was examined at 562 nm by a UV-visible spectrophotometer to calculate their metal chelating capability. Twenty extracted premolars were instrumented and individually soaked in the solutions of 15 wt% EDTA, 10 wt% citric acid, 0.04 wt% ASP, 0.04 wt% FCS, and normal saline were randomly divided into five groups (N=4). Next, each tooth was cleaved longitudinally and examined by scanning electron microscopy (SEM) to assay the effectiveness of smear layer removal. The chelating capability for EDTA, FCS, and ASP showed no significant difference over the concentration of 0.04 wt% (p > 0.05). The SEM results showed that 0.04 wt% FCS solution was effective in smear layer removal along the canal wall. These results indicated that Ganoderma tsuage derived FCS in acid solutions could be a potential alternative as a root canal irrigant solution due to its synergistic effect.

scholarly journals A Comparative Evaluation of Cleaning Efficacy (Debris and Smear Layer Removal) of Hand and Two NiTi Rotary Instrumentation Systems (K3 and ProTaper): A SEM Study

2013 ◽  
Vol 14 (6) ◽  
pp. 1028-1035
Author(s):  
Sangeetha Vallikanthan ◽  
K Balakoti Reddy ◽  
Shreemoy Dash ◽  
Sowmya Kallepalli ◽  
N Chakrapani ◽  
...  
Keyword(s):  
Root Canal ◽  
Comparative Evaluation ◽  
Group Iv ◽  
Smear Layer ◽  
Layer Removal ◽  
Group I ◽  
Rotary Instruments ◽  
Significant Difference ◽  
Sem Study ◽  
Cleaning Efficacy

ABSTRACT Objectives The present study was conducted to compare the cleaning efficacy (debris and smear layer removal) of hand and two NiTi rotary instrumentation systems (K3 and ProTaper). Materials and methods Sixty single rooted human maxillary anterior teeth decoronated at the cementoenamel junction were used. All the specimens were divided into four groups of 15 teeth each, group I—ProTaper rotary instrumentation done, group II—K3 rotary instrumentation done, group III—Stainless steel K-file instrumentation done, group IV—root canal irrigation without instrumentation. Root canal preparation was done in a crown down manner and 3% sodium hypochlorite was used as irrigant after each file followed by final rinse with 5 ml of 17% EDTA solution, then specimens were scanning electron microscopic (SEM) examination. Results Statistical analysis was done using one-way ANOVA followed by post hoc Tukey's HSD test. Group I showed highly statistical significant difference compared to other groups. There was no statistically significant difference considering smear layer at any levels among the groups with no smear layer formation in group IV. Conclusion ProTaper rotary instrumentation showed the maximum cleaning efficacy followed by K3 rotary instrumentation in the coronal, middle and apical thirds of the root canal. Clinical significance ProTaper rotary instruments are more efficient than hand and K3 rotary instruments during root canal treatment. How to cite this article Reddy KB, Dash S, Kallepalli S, Vallikanthan S, Chakrapani N, Kalepu V. A Comparative Evaluation of Cleaning Efficacy (Debris and Smear Layer Removal) of Hand and Two NiTi Rotary Instrumentation Systems (K3 and ProTaper): A SEM Study. J Contemp Dent Pract 2013;14(6):1028-1035.

scholarly journals Evaluation of four final irrigation protocols for cleaning root canal walls

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Qiang Li ◽  
Qian Zhang ◽  
Xiaoying Zou ◽  
Lin Yue
Keyword(s):  
Root Canal ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Smear Layer ◽  
Confocal Laser ◽  
Bacterial Survival ◽  
Bacterial Inhibition ◽  
Layer Removal ◽  
Significant Difference ◽  
Canal Systems

Abstract The aim of this study was to compare the efficiency of four final irrigation protocols in smear layer removal and bacterial inhibition in root canal systems. Thirty roots inoculated with Enterococcus faecalis were prepared with ProTaper Universal files. The teeth were disinfected by conventional needle irrigation, sonic agitation using the EndoActivator device, passive ultrasonic irrigation, or an M3 Max file. Teeth with no root canal preparation served as blank controls for the establishment of the infection baseline. Teeth with preparation but no final irrigation served as a post-instrumentation baseline. After the final irrigation, the teeth were sectioned in half. One half of each tooth was examined by scanning electron microscopy (SEM) to assess smear layer removal using a five-point scale. The other half was examined by confocal laser scanning microscopy (CLSM) using the LIVE/DEAD BackLight bacterial viability kit to evaluate the depth of bacterial survival in dentinal tubules. SEM analysis revealed no significant difference in smear layer removal throughout the whole canal among the EA, PUI, and M3 Max groups (P > 0.05). CLSM revealed that PUI achieved the greatest bacterial inhibition depth in the coronal ((174.27 ± 31.63) μm), middle ((160.94 ± 37.77) μm), and apical ((119.53 ± 28.49) μm) thirds of the canal (all P < 0.05 vs. other groups). According to this comprehensive SEM and CLSM evaluation, PUI appears to have the best infection control ability in root canal systems.

scholarly journals Comparison Between Different Endodontic Irrigating Protocols In Smear Layer Removal From Radicular Dentin

2016 ◽  
Vol 12 (15) ◽  
pp. 38
Author(s):  
Ramona Vlad ◽  
Monika Kovacs ◽  
Dragos Sita ◽  
Mihai Pop
Keyword(s):  
Root Canal ◽  
Endodontic Treatment ◽  
Smear Layer ◽  
Friedman Test ◽  
Cleaning Efficiency ◽  
Layer Removal ◽  
Significant Difference ◽  
System Data ◽  
Root Canal Dentin ◽  
Level Of Significance

The aim of our study was to measure the cleaning efficiency of irrigating solutions used during endodontic treatment regarding smear layer removal from the root canal dentin walls. Ethylenediaminotetraacetic acid (EDTA) 17%, citric acid (CA) 10% and sodium hypochlorite (NaOCl) 2,5 % solutions were tested as final irrigating solutions. The study was conducted on extracted teeth, divided in four groups according to the irrigation protocol used. The specimens were analyzed by scanning electron microscopy and the amount of smear layer present at apical, middle and coronal level was recorded, based on a scoring system. Data were statistically analyzed using Kruskal-Wallis and Friedman test and the level of significance was set at p<0.05. In the coronal and middle segments of dental roots we noticed no statistically significant difference between EDTA and CA in smear layer removing capacity. Final irrigation with 17% EDTA proved to be more efficient than 10% CA and 2,5% NaOCl in smear layer removal at apical level of the root canal, with p<0.05 (p=0.042), which is an important area for disinfection in endodontic treatment.

Efficacy of different final irrigant activation protocols on smear layer removal by EDTA and citric acid

2013 ◽  
Vol 76 (4) ◽  
pp. 364-369 ◽  
Author(s):  
Daniel R. Herrera ◽  
Zarina T. Santos ◽  
Lidia Y. Tay ◽  
Emmanuel J. Silva ◽  
Alessandro D. Loguercio ◽  
...  
Keyword(s):  
Citric Acid ◽  
Smear Layer ◽  
Layer Removal ◽  
Irrigant Activation

scholarly journals Comparative Evaluation Of Antibacterial And Layer Removal Efficacy Of Two Different Herbal Irrigants – An In Vitro Study

2018 ◽  
Vol 1 (11) ◽  
pp. 350-354
Author(s):  
Arvind Kumar ◽  
A Sheerin Sarthaj ◽  
S Maria Antony
Keyword(s):  
Root Canal ◽  
Leaf Extract ◽  
Smear Layer ◽  
Antimicrobial Efficacy ◽  
Ocimum Sanctum ◽  
Layer Removal ◽  
Significant Difference ◽  
The Mean ◽  
Neem Leaf Extract

INTRODUCTION: Irrigation during root canal therapy is especially needed for teeth with complex internal anatomy. Biomechanical preparation of root canal produces "smear layer" which gets embedded with debris and microorganisms. The major side effects of these widely used chemical irrigants such as Sodium hypochlorite for antibacterial efficacy and EDTA for smear layer removal has led to the search of a more biocompatible irrigant.MATERIALS AND METHODS: Azadirachta indica(neem) and Ocimum sanctum(tulsi) extract were used in this study as herbal irrigants to evaluate the above mentioned properties by the q PCR method and SEM analysis.RESULTS: In antimicrobial efficacy, the Mean value of NaOcl showed higher antimicrobial efficacy followed by Neem leaf extract and the lower antimicrobial efficacy was recorded with Tulsi extract. Similarly, the mean smear layer removal by different irrigants at different root levels, EDTA showed the maximum smear layer removal, followed by Neem leaf extract and Tulsi extract showed the least smear layer removal. Hence Tulsi has poor smear layer removal efficacy and comparable antimicrobial efficacy when compared with Neem extractSTATISTICAL ANALYSIS: The collected data were analyzed by one-way analysis of variance to compare the mean of the groups. The post- hoc test (Tukey) was performed to find the interrelationship between different groups for significant difference (P < 0.05; confidence interval 95%).CONCLUSION: From this study, these two herbal irrigants are proved to be safe and effective. It can be concluded that neem leaf extract has a significant antimicrobial efficacy against E. faecalis and significant smear layer removal efficacy compared to 17% EDTA.

scholarly journals Comparative Evaluation of Cleaning Efficacy using Four Novel Nickel–titanium Rotary Instruments: An in vitro Scanning Electron Microscope Study

2017 ◽  
Vol 18 (12) ◽  
pp. 1135-1143
Author(s):  
Prem P Kar ◽  
Sandhya A Khasnis ◽  
Krisnamurthy H Kidiyoor
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Root Canal ◽  
Nickel Titanium ◽  
Smear Layer ◽  
Layer Removal ◽  
Rotary Instruments ◽  
Significant Difference ◽  
Cleaning Efficacy ◽  
Scanning Electron

ABSTRACT Aim The aim of the study was to compare the cleaning efficacy (debris and smear layer removal) of two multifile rotary systems (MTwo and Silk) and two single-file rotary systems (F6 Skytaper and NeoNiTi). Materials and methods Eighty mesial canals of mandibular first molars were cleaned and shaped using four nickel–titanium (NiTi) rotary instruments to size # 25 and 3% NaOCl and 17% ethylenediaminetetraacetic acid (EDTA). Samples were randomly divided into four equal groups (n = 20) according to instrumentation: Group I, Mtwo; group II, Silk; group III, F6 Skytaper; group IV, NeoNiTi. Samples were split longitudinally and examined under scanning electron microscope (SEM) for debris and smear layer removal in coronal, middle, and apical thirds of each root canal. Results F6 skytaper and Mtwo groups showed significantly higher debris removal than Silk and NeoNiTi groups in apical third of root canal as well as when compared with NeoNiTi group in middle third. F6 Skytaper group showed significantly higher debris and smear layer removal than Silk group in coronal third. There was statistically significant difference among all thirds of root canal in terms of debris removal in Silk and NeoNiTi groups. There was statistically significant difference among all thirds of root canal in F6 Skytaper and NeoNiTi groups in terms of smear layer removal. Conclusion F6 Skytaper single-file rotary instrumentation showed the maximum cleaning efficacy followed by Mtwo multifile rotary instrumentation in all thirds of root canal. Clinical significance F6 Skytaper rotary instrument is most efficient followed by Mtwo rotary instrument among all rotary instruments. How to cite this article Kar PP, Khasnis SA, Kidiyoor KH. Comparative Evaluation of Cleaning Efficacy using Four Novel Nickel-titanium Rotary Instruments: An in vitro Scanning Electron Microscope Study. J Contemp Dent Pract 2017;18(12):1135-1143.

scholarly journals Smear Layer Removal for Collagen Fiber Exposure after Citric Acid Conditionings

2010 ◽  
Vol 11 (6) ◽  
pp. 1-8
Author(s):  
Fabio Renato Manzolli Leite ◽  
José Eduardo Cezar Sampaio ◽  
Andrea Abi Rached Dantas ◽  
Rodrigo Cavassim ◽  
Daniela Leal Zandim
Keyword(s):  
Citric Acid ◽  
Collagen Fiber ◽  
Saline Solution ◽  
Collagen Fibers ◽  
Smear Layer ◽  
Control Treatment ◽  
Scanning Electron Micrographs ◽  
Kappa Score ◽  
Layer Removal ◽  
Application Methods

Abstract Aim The aim of the present study was to compare the removal of the smear layer and exposure of collagen fibers of the root surface following the application of five citric acid solution concentrations. Methods and Materials Two hundred seventy (270) samples were equally divided into six groups (n=45) for treatment with saline solution (control) and five different concentrations of citric acid (0.5, 1, 2, 15, and 25 percent). Three acid application methods were used (passive, brushing, and burnishing) as well as three application periods (1, 2, and 3 minutes). A previously trained, calibrated (kappa score = 0.93), and blind examiner subsequently scored scanning electron micrographs (SEMs) of the samples. Statistical analyses were performed by using Kruskal-Wallis and Dunn's post-hoc tests. Results According to the results obtained and within the limitations of the methodology used, the citric acid applications were more effective than the control treatment of applying saline solution (p<0.05). However, no statistically significant differences were observed among the three application methods and three application periods. Descriptive analyses showed that best results for exposure of collagen fibers were obtained with the application of citric acid at 25 percent by brushing for 1 or 3 minutes. Conclusions The best results for exposure of collagen fibers in this study were obtained with application of citric acid at 25 percent by brushing for 1 or 3 minutes, even though there were no statistically significant differences among the groups. Clinical Significance The best results for exposure of collagen fibers on root surfaces noted in this study were obtained with application of citric acid at 25 percent by brushing for 1 or 3 minutes. Citation Cavassim R, Leite FRM, Zandim DL, Dantas AAR, Sampaio JEC. Smear Layer Removal for Collagen Fiber Exposure after Citric Acid Conditionings. J Contemp Dent Pract [Internet]. 2010 December; 11(6):001-008. Available from: http://www.thejcdp.com/journal/view/volume11- issue6-cavassim

scholarly journals Effect of manual dynamic activation with citric acid solutions in smear layer removal: A scanning electron microscopic evaluation

2016 ◽  
Vol 11 (4) ◽  
pp. 360-364
Author(s):  
Juan Gonzalo Olivieri ◽  
Marc García Font ◽  
Eva Stöber ◽  
Joan de Ribot ◽  
Montse Mercadé ◽  
...  
Keyword(s):  
Citric Acid ◽  
Smear Layer ◽  
Acid Solutions ◽  
Electron Microscopic ◽  
Layer Removal ◽  
Microscopic Evaluation ◽  
Scanning Electron Microscopic ◽  
Scanning Electron
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