scholarly journals Electrospun 5-chloro-8-hydroxyquinoline-Loaded Cellulose Acetate/Polyethylene Glycol Antifungal Membranes Against Esca

Polymers ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 1617 ◽  
Author(s):  
Spasova ◽  
Manolova ◽  
Rashkov ◽  
Naydenov
Keyword(s):  
Polyethylene Glycol ◽  
Cellulose Acetate ◽  
Plant Protection ◽  
Bioactive Compound ◽  
Mechanical Tests ◽  
Fibrous Materials ◽  
Water Contact ◽  
Phaeomoniella Chlamydospora ◽  
Optical And Mechanical Properties ◽  
Surface Morphologies

Esca is one of the earliest described diseases in grapevines and causes trunk damage and the sudden wilting of the entire plant; it is caused mainly by the species Phaeomoniella chlamydospora (P. chlamydospora) and Phaeoacremonium aleophilum (P. aleophilum). In practice, there are no known curative approaches for fighting esca directly, which is a huge problem for preserving vineyards. Micro- and nanofibrous membranes from cellulose acetate (CA) and cellulose acetate/polyethylene glycol (CA/PEG) containing 5-chloro-8-hydroxyquinolinol (5-Cl8Q) were successfully prepared by electrospinning. The surface morphologies and optical and mechanical properties of the membranes were characterized by using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), ultraviolet-visible spectroscopy (UV-Vis), water contact angle measurements and mechanical tests. It was found that the bioactive compound release was facilitated by PEG. The antifungal activities of the obtained materials against P. chlamydospora and P. aleophilum were studied. We have demonstrated that 5-Cl8Q is an efficient and sustainable antifungal agent against P. chlamydospora and P. aleophilum. Moreover, for the first time, the present study reveals the possibility of using electrospun polymer membranes containing 5-Cl8Q which impede the penetration and growth of P. chlamydospora and P. aleophilum. Thus, the obtained fibrous materials can be suitable candidates for plant protection against diverse fungi.

scholarly journals Cellulose Acetate-Based Electrospun Materials with a Variety of Biological Potentials: Antibacterial, Antifungal and Anticancer

Polymers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1631
Author(s):  
Mariya Spasova ◽  
Nevena Manolova ◽  
Iliya Rashkov ◽  
Petya Tsekova ◽  
Ani Georgieva ◽  
...  
Keyword(s):  
Cellulose Acetate ◽  
Mechanical Tests ◽  
Fibrous Materials ◽  
Water Contact ◽  
Antibacterial And Antifungal Activities ◽  
3T3 Fibroblasts ◽  
Angle Measurements ◽  
Zones Of Inhibition ◽  
Model Drug ◽  
Antibacterial And Antifungal

Novel eco-friendly fibrous materials with complex activities from cellulose acetate and cellulose acetate/polyethylene glycol (CA,PEG) containing 5-chloro-8-hydroxyquinoline as a model drug were obtained by electrospinning. Several methods, including scanning electron microscopy, X-ray diffraction analysis, ultraviolet-visible spectroscopy, water contact angle measurements, and mechanical tests, were utilized to characterize the obtained materials. The incorporation of PEG into the fibers facilitated the drug release. The amounts of the released drug from CA/5-Cl8Q and CA,PEG/5-Cl8Q were 78 ± 3.38% and 86 ± 3.02%, respectively (for 175 min). The antibacterial and antifungal activities of the obtained materials were studied. The measured zones of inhibition of CA/5-Cl8Q and CA,PEG/5-Cl8Q mats were 4.0 ± 0.18 and 4.5 ± 0.2 cm against S. aureus and around 4.0 ± 0.15 and 4.1 ± 0.22 cm against E. coli, respectively. The complete inhibition of the C. albicans growth was detected. The cytotoxicity of the obtained mats was tested toward HeLa cancer cells, SH-4 melanoma skin cells, and mouse BALB/c 3T3 fibroblasts as well. The CA/5-Cl8Q and CA,PEG/5-Cl8Q materials exhibited anticancer activity and low normal cell toxicity. Thus, the obtained fibrous materials can be suitable candidates for wound dressing applications and for application in local cancer treatment.

scholarly journals Electrospun Eco-Friendly Materials Based on Poly(3-hydroxybutyrate) (PHB) and TiO2 with Antifungal Activity Prospective for Esca Treatment

Polymers ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1384
Author(s):  
Mariya Spasova ◽  
Olya Stoilova ◽  
Nevena Manolova ◽  
Iliya Rashkov ◽  
Mladen Naydenov
Keyword(s):  
Electron Microscopy ◽  
Antifungal Activity ◽  
Fungal Growth ◽  
Fibrous Materials ◽  
One Pot ◽  
Water Contact ◽  
Tio2 Anatase ◽  
Phaeomoniella Chlamydospora ◽  
Design Type ◽  
Nanosized Tio2

Esca is a type of grapevine trunk disease that severely affects vine yield and longevity. Phaeomoniella chlamydospora (P. chlamydospora) is one of the main fungi associated with esca. The aim of the present study was to obtain eco-friendly materials with potential antifungal activity against P. chlamydospora based on biodegradable and biocompatible poly(3-hydroxybutyrate) (PHB), nanosized TiO2-anatase (nanoTiO2), and chitosan oligomers (COS) by conjunction of electrospinning and electrospraying. One-pot electrospinning of a suspension of nanosized TiO2 nanoparticles in PHB solution resulted in materials in which TiO2 was incorporated within the fibers (design type “in”). Simultaneous electrospinning of PHB solution and electrospraying of the dispersion of nanosized TiO2 in COS solution enabled the preparation of materials consisting of PHB fibers on which TiO2 was deposited on the fibers’ surface (design type “on”). Several methods including scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction analysis (XRD), thermogravimetric analyses (TGA) and water contact angle were utilized to characterize the obtained materials. The incorporation of nanoTiO2 in the PHB fibers, as well as nanoTiO2 deposition onto the surface of the PHB fibers resulted in increased roughness and hydrophobicity of the obtained composite fibrous materials. Moreover, TiO2-on-PHB fibrous material exhibited complete inhibition of fungal growth of P. chlamydospora. Therefore, the obtained eco-friendly fibrous materials based on PHB and nanoTiO2 are promising candidates for protection against esca in agriculture.

scholarly journals Humidity-Responsive Photonic Films and Coatings Based on Tuned Cellulose Nanocrystals/Glycerol/Polyethylene Glycol

Polymers ◽  
2021 ◽  
Vol 13 (21) ◽  
pp. 3695
Author(s):  
Amin Babaei-Ghazvini ◽  
Bishnu Acharya
Keyword(s):  
Polyethylene Glycol ◽  
Water Uptake ◽  
Cellulose Nanocrystals ◽  
Color Change ◽  
Colloidal Suspensions ◽  
Mechanical Tests ◽  
Humidity Sensors ◽  
Water Contact ◽  
Structural Colors ◽  
Mesoporous Film

It has been extensively reported that cellulose nanocrystals (CNCs) can represent structural colors due to their unique chiral-nematic self-assembly. However, the application of this remarkable structure does need further investigation. It has been challenging to keep the selective reflection band (SRB) resulting from the CNC structure in the visible spectrum. Herein, composition of CNC colloidal suspensions with polyethylene glycol (PEG) and glycerol (Gly) have been studied to develop humidity-responsive sensors in the form of coatings and films. The fabricated samples were characterized for their mechanical properties, optical properties, water uptake capacity, water contact angle, and surface roughness. Additionally, the chemical structure of the samples was studied with FTIR spectroscopy. The produced humidity indicators on microbial glass slides were maintained and tested in a different relative humidity range from 20% to 98% with a different color response from blue to red, respectively. The color change of the humidity sensors was reversible for several cycles. It should be noted that the color change can be detected easily by the naked eye. The water uptake test showed that pure CNC and CNC/Gly had the lowest (34%) and highest (83%) water absorption levels. The mechanical tests for CNC/PEG composites showed the highest tensile strength (40.22 MPa). Moreover, microstructural characterizations confirmed the CNC pitch formation in all the samples. Addition of the fillers increased the CNC pitch, resulting in a mesoporous film formation. These produced humidity sensors are promising candidates in food and drug packaging due to their biodegradability, biocompatibility, and cost-effectiveness.

Development, Evaluation and Optimization of Osmotic Controlled Tablets of Aceclofenac for Rheumatoid Arthritis Management

2019 ◽  
Vol 9 (1) ◽  
pp. 29-36
Author(s):  
Bijaya Ghosh ◽  
Niraj Mishra ◽  
Preeta Bose ◽  
Moumita D. Kirtania
Keyword(s):  
Rheumatoid Arthritis ◽  
Polyethylene Glycol ◽  
Sodium Chloride ◽  
Cellulose Acetate ◽  
Hydroxypropyl Methylcellulose ◽  
Release Profile ◽  
Polyethylene Glycol 400 ◽  
Permeable Membrane ◽  
Cumulative Release ◽  
The Core

Objective: Rheumatoid arthritis is a dreaded disease, characterized by pain, inflammation and stiffness of joints, leading to severe immobility problems. The disease shows circadian variation and usually gets aggravated in early morning hours. Aceclofenac, a BCS Class II compound is routinely used in the treatment of pain and inflammation associated with rheumatoid arthritis. The objective of this study was to develop an osmotic delivery system of Aceclofenac that after administration at bedtime would deliver the drug in the morning hours. </P><P> Methods: A series of osmotically controlled systems of aceclofenac was developed by using lactose, sodium chloride and hydroxypropyl methylcellulose K100M as osmogens. Cellulose acetate (2% w/v in acetone) with varying concentrations of polyethylene glycol-400 was used as the coating polymer to create semi permeable membrane and dissolution was carried out in 290 mOsm phosphate buffer. Formulation optimization was done from four considerations: cumulative release at the end of 6 hours (lag time), cumulative release at the end of 7 hours (burst time), steady state release rate and completeness of drug release. </P><P> Results: A formulation having swelling polymer hydroxypropyl methylcellulose in the core and lactose and sodium chloride as osmogens, polyethylene glycol-400 (16.39 %) as pore former, with a coating weight of 5% was a close fit to the target release profile and was chosen as the optimum formulation. Conclusion: Aceclofenac tablets containing lactose, HPMC and sodium chloride in the core, given a coating of cellulose acetate and PEG-400 (5% wt gain), generated a release profile for optimum management of rheumatoid arthritic pain.

scholarly journals PLA-Based Hybrid and Composite Electrospun Fibrous Scaffolds as Potential Materials for Tissue Engineering

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Anna Magiera ◽  
Jarosław Markowski ◽  
Elzbieta Menaszek ◽  
Jan Pilch ◽  
Stanislaw Blazewicz
Keyword(s):  
Tissue Engineering ◽  
Mechanical Tests ◽  
Hybrid Structures ◽  
Poly Lactic Acid ◽  
Electrospinning Technique ◽  
Water Contact ◽  
Human Osteoblasts ◽  
Angle Measurements ◽  
Contact Angle Measurements ◽  
Normal Human

The aim of the study was to manufacture poly(lactic acid)- (PLA-) based nanofibrous nonwovens that were modified using two types of modifiers, namely, gelatin- (GEL-) based nanofibres and carbon nanotubes (CNT). Hybrid nonwovens consisting of PLA and GEL nanofibres (PLA/GEL), as well as CNT-modified PLA nanofibres with GEL nanofibres (PLA + CNT/GEL), in the form of mats, were manufactured using concurrent-electrospinning technique (co-ES). The ability of such hybrid structures as potential scaffolds for tissue engineering was studied. Both types of hybrid samples and one-component PLA and CNTs-modified PLA mats were investigated using scanning electron microscopy (SEM), water contact angle measurements, and biological and mechanical tests. The morphology, microstructure, and selected properties of the materials were analyzed. Biocompatibility and bioactivity in contact with normal human osteoblasts (NHOst) were studied. The coelectrospun PLA and GEL nanofibres retained their structures in hybrid samples. Both types of hybrid nonwovens were not cytotoxic and showed better osteoinductivity in comparison to scaffolds made from pure PLA. These samples also showed significantly reduced hydrophobicity compared to one-component PLA nonwovens. The CNT-contained PLA nanofibres improved mechanical properties of hybrid samples and such a 3D system appears to be interesting for potential application as a tissue engineering scaffold.

scholarly journals Nanocomposite Film Based on Cellulose Acetate and Lignin-Rich Rice Straw Nanofibers

Materials ◽  
2019 ◽  
Vol 12 (4) ◽  
pp. 595 ◽  
Author(s):  
Mohammad Hassan ◽  
Linn Berglund ◽  
Ragab Abou-Zeid ◽  
Enas Hassan ◽  
Wafaa Abou-Elseoud ◽  
...  
Keyword(s):  
Contact Angle ◽  
Cellulose Acetate ◽  
Rice Straw ◽  
Water Vapor Permeability ◽  
Light Transmittance ◽  
Vapor Permeability ◽  
Water Contact ◽  
Transparent Films ◽  
Force Microscopy ◽  
Good Transparency

Nanofibers isolated from unbleached neutral sulfite rice straw pulp were used to prepare transparent films without the need to modify the isolated rice straw nanofibers (RSNF). RSNF with loading from 1.25 to 10 wt.% were mixed with cellulose acetate (CA) solution in acetone and films were formed by casting. The films were characterized regarding their transparency and light transmittance, microstructure, mechanical properties, crystallinity, water contact angle, porosity, water vapor permeability, and thermal properties. The results showed good dispersion of RSNF in CA matrix and films with good transparency and homogeneity could be prepared at RSNF loadings of less than 5%. As shown from contact angle and atomic force microscopy (AFM) measurements, the RSNF resulted in increased hydrophilic nature and roughness of the films. No significant improvement in tensile strength and Young’s modulus was recorded as a result of adding RSNF to CA. Addition of the RSNF did not significantly affect the porosity, crystallinity and melting temperature of CA, but slightly increased its glass transition temperature.

scholarly journals INCORPORATION OF IMPRINTED-ZEOLITE TO POLYETHERSULFONE/CELLULOSE ACETATE MEMBRANE FOR CREATININE REMOVAL IN HEMODIALYSIS TREATMENT

2019 ◽  
Vol 81 (3) ◽  
Author(s):  
Yanuardi Raharjo ◽  
Mochamad Zakki Fahmi ◽  
Siti Wafiroh ◽  
Alfa Akustia Widati ◽  
Eviomitta Rizki Amanda ◽  
...  
Keyword(s):  
Cellulose Acetate ◽  
Pure Water ◽  
Water Flux ◽  
Hollow Fibre ◽  
Mixed Matrix Membranes ◽  
Mixed Matrix Membrane ◽  
Cellulose Acetate Membrane ◽  
Zeolite A ◽  
Mixed Matrix ◽  
Water Contact

Polyethersulfon (PES) membrane has been widely used in the biomedical field especially in hemodialysis application. Many modifications of membranes have been applied into hemodialysis such as diffusion, adsorption, and mixed-matrix membrane. The main problem of those membranes is less selectivity to attract the uremic toxins. In this study, we report the modification of PES mixed with cellulose acetate (PES/CA) membrane as mixed-matrix membrane (MMM) using imprinted-zeolite (PES/CA/IZC) in order to increase the selectivity for targeted analyte. The hollow fibre membranes (HFM) were fabricated by dry-wet spinning technique. The successful zeolite A synthesised and was characterised by x-ray diffraction (XRD). The mixed-matrix membranes were characterised in terms of morphology using scanning electron microscopy (SEM), water contact angle (WCA), pure water flux (PWF), clearance of creatinine (CC), and BSA adsorption. In accordance with the results of characterisation, the synthesis of zeolite A, and imprinted-zeolite creatinine was successfully fabricated. The SEM results showed that the PES/CA/IZC membrane has uniform pores and fingerlike structure. The same result was obtained for PES/CA membrane, but not for PES/CA/ZA membrane. The WCA of the PES/CA; PES/CA/ZA; and PES/CA/IZC were 85.63; 84.98; and 77.53 (o), respectively. While the PWF were 22.84; 27.57, and 40.52 (Lm-2h-1), respectively. The addition of imprinted-zeolite into the membrane improved creatinine removal up to 74.99%. It showed that PES/CA/IZC has succeeded in increasing the selectivity of membranes to attract the creatinine as target analyte. Compared to the PES/CA, the creatinine clearance of membranes improved and increased up to 5.2%. For protein rejection, the PES/CA/IZC rejected 79.05% of bovine serum albumin (BSA). Based on these results, it can be concluded that PES/CA/IZC can be considered as hemodialysis membranes.

Study on Preparation and Surface Properties of Fluorinated Acrylate Hydrophobic Polymer Film

2015 ◽  
Vol 1088 ◽  
pp. 101-106
Author(s):  
Jing Chen ◽  
Qian Cheng ◽  
Shun Quan Chen
Keyword(s):  
Contact Angle ◽  
Water Content ◽  
Polymer Film ◽  
Water Contact ◽  
Fluorinated Acrylate ◽  
Fluorinated Polyacrylate ◽  
Hydrophobic Polymer ◽  
Air Interface ◽  
Surface Morphologies ◽  
Lower Water Content

A new fluorinated acrylate terpolymer hydrophobic polymer was prepared by emulsifier-free inverse microemulsion polymerization using methyl methacrylate (MMA) and hexafluorobutyl acrylate (HFBA) as monomers, and methyl polyethylene glycol methacrylate (MPEGMA) as polymerizable emulsifier. Membrane properties were characterized by means of FT-IR, SEM and Contact Angle Meter. The effect of fluorinated monomer content, water content in the inverse precursor microemulsion on the surface morphologies and hydrophobicity of the polymer film was mainly discussed. Morphological studies of the fluorinated polyacrylate polymers by SEM showed that discrete granular microstructures were obtained from precursor microemulsion with higher water content. On the other hand, smooth and continuous surface morphologies were found with lower water content. Contact angle revealed that the fluorine preferentially enriched at the film-air interface and more hydrophobic that the film-glass interface. The amount of HFBA had significantly effects on the film properties. The water contact angle of the film-air interface increased as the amount of HFBA increased from 0 ml to 4 ml and then remained almost unchangeable. The film formed from the fluorinated polyacrylate polymer with higher water content in the precursor microemulsion exhibited better hydrophobicity in comparison with the film formed from the fluorinated polyacrylate polymer with the lower water content, and it confirmed that rough surface have high water contact angles.

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