scholarly journals Biocompatibility of Polymer and Ceramic CAD/CAM Materials with Human Gingival Fibroblasts (HGFs)

Polymers ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 1446 ◽  
Author(s):  
Rizo-Gorrita ◽  
Herráez-Galindo ◽  
Torres-Lagares ◽  
Serrera-Figallo ◽  
Gutiérre-Pérez
Keyword(s):  
Collagen Type ◽  
Tetragonal Zirconia ◽  
Collagen Type I ◽  
Human Gingival Fibroblasts ◽  
Lithium Silicate ◽  
Type I ◽  
Gingival Fibroblasts ◽  
Collagen Secretion ◽  
Computer Aided ◽  
Cad Cam

Four polymer and ceramic computer-aided design/computer-aided manufacturing (CAD/CAM) materials from different manufacturers (VITA CAD-Temp (polymethyl methacrylate, PMMA), Celtra Duo (zirconia-reinforced lithium silicate ceramic, ZLS), IPS e.max CAD (lithium disilicate (LS2)), and VITA YZ (yttrium-tetragonal zirconia polycrystal, Y-TZP)) were tested to evaluate the cytotoxic effects and collagen type I secretions on human gingival fibroblasts (HGFs). A total of 160 disc-shaped samples (Ø: 10 ± 2 mm; h: 2 mm) were milled from commercial blanks and blocks. Direct-contact cytotoxicity assays were evaluated at 24, 48, and 72 h, and collagen type I (COL1) secretions were analysed by cell-based ELISA at 24 and 72 h. Both experiments revealed statistically significant differences (p < 0.05). At 24 and 48 h of contact, cytotoxic potential was observed for all materials. Later, at 72 h, all groups reached biologically acceptable levels. LS2 showed the best results regarding cell viability and collagen secretion in all of the time evaluations, while Y-TZP and ZLS revealed intermediate results, and PMMA exhibited the lowest values in both experiments. At 72 h, all groups showed sharp decreases in COL1 secretion regarding the 24-h values. According to the results obtained and the limitations of the present in vitro study, it may be concluded that the ceramic materials revealed a better cell response than the polymers. Nevertheless, further studies are needed to consolidate these findings and thus extrapolate the results into clinical practice.

Bacterial Endotoxin Inhibits Migration, Attachment, and Orientation of Human Gingival Fibroblasts in vitro and Delays Collagen Gel Contraction

1987 ◽  
Vol 66 (9) ◽  
pp. 1449-1455 ◽  
Author(s):  
S. Pitaru ◽  
M. Soldinger ◽  
D. Madgar ◽  
Z. Metzger
Keyword(s):  
Collagen Type ◽  
Cell Attachment ◽  
Collagen Gel ◽  
Collagen Type I ◽  
Human Gingival Fibroblasts ◽  
Bacterial Endotoxin ◽  
Type I ◽  
Gingival Fibroblasts ◽  
Collagen Gels

The purpose of this study was to assess the effect of endotoxin adsorbed to dental surfaces and to collagen type I on the migration, attachment, and orientation of human gingival fibroblasts (HGF). Transversely cut porcine tooth root slices (RS), 200 μm thick, were prepared. Half of the RS obtained were partially demineralized in EDTA. Half of the demineralized and non-demineralized RS were incubated with 400 μg/mL of endotoxin for 24 hr, whereas the other half were maintained in PBS and served as controls. Experimental and control RS were placed on confluent layers of HFG and cultured for six days. Cell migration toward and cell attachment to the periphery of the RS and the formation of oriented cell sheets were assessed by means of photographic techniques. Additionally, six-day-old cultures were fixed and processed for SEM observation. In separate experiments, the effect of endotoxin on cell attachment to collagen type I and on contraction of three-dimensional collagen gels was assessed. It was found that: (i) bacterial endotoxin inhibited migration and attachment of HGF to both demineralized and non-demineralized cementum and interfered with the development of oriented cellular structure ; (ii) the inhibitory effect was significantly more pronounced for non-demineralized than for demineralized cementum; (iii) the morphology of HGF attached to endotoxin-treated dental surfaces was altered compared with that of their controls; and (iv) bacterial endotoxin inhibited cell attachment to collagen type I and delayed the contraction of collagen gel.

Attachment, proliferation and collagen type I mRNA expression of human gingival fibroblasts on different biodegradable membranes

2013 ◽  
Vol 54 (4-5) ◽  
pp. 260-266 ◽  
Author(s):  
Sema S. Hakki ◽  
Petek Korkusuz ◽  
Nuhan Purali ◽  
Buket Bozkurt ◽  
Mahmut Kus ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Collagen Type ◽  
Collagen Type I ◽  
Human Gingival Fibroblasts ◽  
Type I ◽  
Gingival Fibroblasts

Behavior of Osteoblasts Cultured on Collagen Coated Bioactive Glass

2005 ◽  
Vol 284-286 ◽  
pp. 675-678 ◽  
Author(s):  
A.L. Andrade ◽  
Patricia Valério ◽  
Alfredo Goes ◽  
M. Fatima Leite ◽  
Rosana Z. Domingues
Keyword(s):  
Alkaline Phosphatase ◽  
Chemical Process ◽  
Collagen Type ◽  
Sol Gel ◽  
Collagen Type I ◽  
Type I ◽  
Cellular Viability ◽  
Collagen Secretion ◽  
Sol Gel Process ◽  
Alkaline Phosphate

In the current work, we investigated cellular viability, proliferation, and metabolic activity of rat primary culture osteoblasts in contact with a sample of collagen type I (C) and of this same collagen chemically treated (CTP). The chemical process used here consisted in recover the collagen surface with silica glass obtained from a sol-gel process. The cell viability, the cell death, the alkaline phosphate production and collagen secretion, after 72 hours of incubating the samples with osteoblasts, were measured by MTT assay, propidium iodide, NBT-BCIP assay and SIRCOL method, respectively. The viability and proliferation of osteoblasts had a significant decrease in the presence of samples when compared to control. The alkaline phosphatase production by the cells had a significant increase in the presence of CTP and collagen secretion by the cells was practically the same when compared to control.

Effects of Manufacturing and Finishing Techniques of Feldspathic Ceramics on Surface Topography, Biofilm Formation, and Cell Viability for Human Gingival Fibroblasts

2018 ◽  
Vol 43 (6) ◽  
pp. 593-601 ◽  
Author(s):  
LPC Contreras ◽  
AMO Dal Piva ◽  
FC Ribeiro ◽  
LC Anami ◽  
SEA Camargo ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Computer Aided Design ◽  
Three Dimensional ◽  
Human Gingival Fibroblasts ◽  
Cytotoxicity Test ◽  
Gingival Fibroblasts ◽  
Computer Aided ◽  
Cad Cam ◽  
Diphenyl Tetrazolium Bromide ◽  
Ceramic Restorations

SUMMARY Purpose: Feldspathic ceramic restorations can be obtained by different techniques (stratification or computer-aided design/computer-aided manufacturing [CAD/CAM] blocks) and finishing procedures (polishing or glaze application). This study evaluated the effects of techniques and finishing procedures on surface properties, biofilm formation, and viability of human gingival fibroblasts (FMM-1) in contact with these materials. Methods and Materials: Ceramic specimens were obtained through a stratification technique (Vita VM9) and from CAD/CAM blocks (Vita Blocs Mark II; both Vita Zahnfabrik) and their surfaces were finished by polishing (ceramisté diamond rubbers + polishing paste; “p” subgroups) or glaze spray application + sintering (“g” subgroups). Roughness (Ra and RSm parameters) and surface free energy (SFE) were measured. Early biofilm formation of Streptococcus mutans, Streptococcus sanguinis, and Candida albicans was evaluated by counting colony-forming units (CFU). MTT (3-[4.5-dimethyl-thiazol-2-yl-]-2.5-diphenyl tetrazolium bromide) cytotoxicity test evaluated cellular viability for the growth of FMM-1 after 24 hours and seven days of contact. Scanning electron microscopy (SEM) and three-dimensional optical profilometry were performed to qualitatively analyze the surface. Data were analyzed by analysis of variance, Tukey test, and t-test (all α=0.05). Results: Polished samples presented lower roughness (Ra, p=0.015; RSm, p=0.049) and higher SFE (p=0.00). Streptococci had higher CFU in all groups, but the CFU of C albicans was lower for polished samples. Biofilm formation was influenced by the interaction of all factors (p=0.018), and the materials showed no cytotoxicity to FMM-1 growth. Conclusions: Polishing resulted in the lowest values for surface roughness and higher SFE values. Polished ceramics showed less C albicans adherence while the adherence of Streptococci was greater than C albicans in all conditions.

The effect of centrifugal force on the transcription levels of collagen type I and collagenase in cultured canine gingival fibroblasts

1998 ◽  
Vol 43 (4) ◽  
pp. 313-316 ◽  
Author(s):  
Meir Redlich ◽  
Aaron Palmon ◽  
Batya Zaks ◽  
Edel Geremi ◽  
Sofia Rayzman ◽  
...  
Keyword(s):  
Centrifugal Force ◽  
Collagen Type ◽  
Collagen Type I ◽  
Type I ◽  
Gingival Fibroblasts

TGF-β-regulated collagen type I accumulation: role of Src-based signals

2007 ◽  
Vol 292 (4) ◽  
pp. C1361-C1369 ◽  
Author(s):  
Rangnath Mishra ◽  
Ling Zhu ◽  
Richard L. Eckert ◽  
Michael S. Simonson
Keyword(s):  
Type I Collagen ◽  
Collagen Type ◽  
Transforming Growth Factor ◽  
Mesangial Cells ◽  
Collagen Type I ◽  
Dominant Negative ◽  
Dominant Negative Mutant ◽  
Type I ◽  
Small Interference Rna ◽  
Collagen Secretion

Transforming growth factor-β (TGF-β) stimulates myofibroblast transdifferentiation, leading to type I collagen accumulation and fibrosis. We investigated the function of Src in TGF-β-induced collagen I accumulation. In human mesangial cells, PTyr416 Src (activated Src) was 3.3-fold higher in TGF-β-treated cells than in controls. Src activation by TGF-β was blocked by rottlerin and by a dominant negative mutant of protein kinase Cδ (PKCδ), showing that TGF-β activates Src by a PKCδ-based mechanism. Pharmacological inhibitors and a dominant negative Src mutant prevented the increase in collagen type I secretion in cells exposed to TGF-β. Similarly, on-target Src small interference RNA (siRNA) prevented type I collagen secretion in response to TGF-β, but off-target siRNA complexes had no effect. It is well established in mesangial cells that upregulation of type I collagen by TGF-β requires extracellular signal-regulated kinase 1/2 (ERK1/2), and we found that activation of ERK1/2 by TGF-β requires Src. In conclusion, these results suggest that stimulation of collagen type I secretion by TGF-β requires a PKCδ-Src-ERK1/2 signaling motif.

scholarly journals Effect of injectable equine collagen type I on metabolic activity and apoptosis of gingival fibroblasts

2020 ◽  
Author(s):  
Sylwia Klewin-Steinböck ◽  
Agnieszka Nowak-Terpiłowska ◽  
Zygmunt Adamski ◽  
Katarzyna Grocholewicz ◽  
Marzena Wyganowska-Świątkowska
Keyword(s):  
Metabolic Activity ◽  
Collagen Type ◽  
Collagen Type I ◽  
Type I ◽  
Gingival Fibroblasts

scholarly journals P067 The effect of a probiotic mix on mucosal healing and fibrotic responses in healthy colonic subepithelial myofibroblasts

2021 ◽  
Vol 15 (Supplement_1) ◽  
pp. S172-S173
Author(s):  
G Tarapatzi ◽  
E Filidou ◽  
L Kandilogiannakis ◽  
K Arvanitidis ◽  
S Vradelis ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Culture Medium ◽  
Collagen Type ◽  
Collagen Type I ◽  
Mucosal Healing ◽  
Type I ◽  
Healthy Individuals ◽  
Collagen Secretion ◽  
Probiotic Strains ◽  
Migration Capacity

Abstract Background Various probiotic strains play a positive role in Inflammatory Bowel Disease and might support mucosal healing. Colonic Subepithelial Myofibroblasts (SEMFs) have a pivotal role in mucosal healing and fibrosis, by migrating to the trauma region and secreting collagen. Our study aimed to investigate whether different strains of probiotics can affect the migration capacity and the fibrotic phenotype of SEMFs isolated from healthy individuals. Methods The probiotic mix of Lactobacillus acidophilus, Lactobacillus plantarum, Bifidobacterium lactis and Saccharomyces boulardii was reconstituted in SEMFs culture medium, they were identified by Gram staining and their viability was assessed by Trypan Blue staining. Primary SEMFs were isolated from colonic biopsies from healthy individuals, and stimulated with 102, 104 or 108 cfu/ml of the mix for 6h or 48h; in 6h total RNA was collected and mRNA expression of collagen type I, III, fibronectin, tissue factor (TF) and α-SMA was measured by quantitative PCR, and in 48h their effect on migration rate through the Wound Healing Assay and collagen secretion via Sircol was assessed. Results Trypan Blue confirmed that the majority of the probiotic mix was alive, and all three bacterial strains and the yeast were stained Gram positive. Stimulation of SEMFs with the probiotic mix resulted in different effects depending on its concentration; 102 cfu/ml downregulated protein collagen secretion (3.4μg/ml, IQR: 2.7–5.0, p&lt;0.01 vs control 6.3μg/ml, IQR: 5.2–6.7) and α-SMA mRNA production (0.7-fold, IQR: 0.7–0.8, p&lt;0.01), 104 cfu/ml upregulated the Fibronectin mRNA expression (1.6-fold, IQR: 1.2–1.8, p&lt;0.0001) and 108 cfu/ml downregulated collagen type I (0.2-fold, IQR: 0.1–0.8, p&lt;0.01) and III mRNAs (0.3-fold, IQR: 0.1–0.8, p&lt;0.01). In addition, all three concentrations resulted in the overall upregulation of TF mRNA expression. Regarding the effect of the probiotic mix on SEMFs migration capacity, 104 cfu/ml of the probiotic mix resulted in increased migration rate by 133.8% (IQR: 102.8–150.2, p&lt;0.01) after 24h, while the use of 102 cfu/ml and 108 cfu/ml decreased it by 58.2% (IQR: 34.8–79.7, p&lt;0.01) and by 46.8% (IQR: 34.3–72.1, p&lt;0.01) respectively. Conclusion This study provides evidence that the probiotic strains L. plantarum, L. acidophillus, B. lactis and S. boulardii remain viable in SEMFs’ culture medium and have the ability to interact with and alter the behavior of SEMFs. Moreover, the effect of the probiotic mix on SEMFs fibrotic phenotype is dependent of the concentration of the probiotics used. Further preclinical research is needed in order to identify the effect of each strain, as well as the suitable dose and combinations in various pathological conditions.

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