scholarly journals Poly-Gamma-Glutamic Acid Functions as an Eective Lubricant with Antimicrobial Activity in Multipurpose Contact Lens Care Solutions

Polymers ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 1050 ◽  
Author(s):  
Chen-Ying Su ◽  
Ching-Li Tseng ◽  
Shu-Hsuan Wu ◽  
Bo-Wu Shih ◽  
Yi-Zhou Chen ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Glutamic Acid ◽  
Chlorine Dioxide ◽  
Contact Lens ◽  
Synergetic Effect ◽  
Main Ingredient ◽  
Functional Polymer ◽  
Multiple Functions ◽  
Lubricating Property ◽  
And Storage

In order to perform the multiple functions of disinfection, cleansing, and storage, preservatives are often added to contact lens care solutions. The disadvantage of adding preservatives is that this often causes various eye conditions. However, lens care solutions would not be able to disinfect in the absence of such preservatives. In addition, comfort is an important issue for contact lens wearers due to the long periods of time they are worn. It has been shown that lower friction coefficients are correlated with increased comfort. We have previously developed a multipurpose contact lens care solution in which poly-gamma-glutamic acid (γ-PGA) was the main ingredient. In this study, we investigated the antimicrobial activity and lubricating property of our care solution. We showed that there was a synergetic effect of γ-PGA and chlorine dioxide on antimicrobial activity. We also demonstrated that γ-PGA functioned as a lubricating agent. Our results provided evidence of γ-PGA acting as a multi-functional polymer that could be applied in contact lens care solutions.

scholarly journals The Bio-Tribological Effect of Poly-Gamma-Glutamic Acid in the Lysozyme-Ionic Contact Lens System

Polymers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 156
Author(s):  
Chen-Ying Su ◽  
Lung-Kun Yeh ◽  
Chi-Chun Lai ◽  
Mihaela Dubuisson ◽  
Yi-Fei Tsao ◽  
...  
Keyword(s):  
Friction Coefficient ◽  
Glutamic Acid ◽  
Contact Lens ◽  
Contact Lenses ◽  
Lens System ◽  
Friction Testing ◽  
Lubricating Property ◽  
Velocity Variations

Feeling comfortable is an important issue for contact lens wearers as contact lenses are worn for an extensive period of time. It has been shown that the in vitro friction coefficient of contact lenses is correlated to the degree of in vivo comfort, thus many studies focus on establishing friction testing methods for investigating the friction coefficient of contact lenses or contact lens care solution. We have previously demonstrated the lubricating property of poly-gamma-glutamic acid (γ-PGA)-containing care solution, and it could reduce the high friction coefficient caused by lysozyme. However, the mechanism of how γ-PGA-containing care solution reduces the lysozyme-induced friction coefficient of contact lenses is unclear. We investigated the bio-tribological effect of γ-PGA on ionic contact lenses in the presence of lysozyme by testing load and velocity variations. The ability to remove lysozyme deposition by γ-PGA and viscosity analysis of γ-PGA-containing care solutions were also investigated to understand the potential mechanism. Our results showed that the friction coefficient of γ-PGA-containing care solution with lysozyme was the lowest in both load and velocity variations, and γ-PGA functions distinctly in the lysozyme-ionic contact lens system. We proposed a model of how γ-PGA could reduce the friction coefficient in these two conditions.

Underestimated risks for bacterial keratitis in contact lens usage

2021 ◽  
Vol 26 (3) ◽  
pp. 2626-2630
Author(s):  
EVELINA MILCHEVA MARINOVA ◽  
◽  
DIMITAR STOIMENOV DABOV ◽  
YANI TODOROV ZDRAVKOV ◽  
EMILIA KRASIMIROVA NASEVA ◽  
...  
Keyword(s):  
Risk Factors ◽  
Contact Lens ◽  
Contact Lenses ◽  
Negative Impact ◽  
Bacterial Keratitis ◽  
Ophthalmic Examination ◽  
The Social ◽  
And Storage ◽  
Predisposing Conditions

Objective: The purpose of our research is to emphasize the role of underestimated predisposing conditions for microbial keratitis in contact lens users, to draw attention to the complications of wearing contact lenses without ophthalmic examination, and to offer a short questionnaire for fast and easy estimation of the risk factors for bacterial keratitis. Methods: A series of 23 cases of healthy young individuals, treated for bacterial keratitis, provoked а research on the predisposing factors for its development. Results: 55% of the patients had started to wear contact lenses without specific examination. 75% had made mistakes in the hygiene and storage of the lenses. We identified risk factors for development of bacterial keratitis in all cases. Conclusions: A thorough anamnesis and ophthalmic examination are crucial in decision making about contact lens wearing because they could reveal some of the predisposing conditions for the occurrence of ocular complications. The usage of contact lenses without a prescription poses health risks and could have a negative impact on the social and healthcare system.

scholarly journals Development of Novel Thin Polycaprolactone (PCL)/Clay Nanocomposite Films with Antimicrobial Activity Promoted by the Study of Mechanical, Thermal, and Surface Properties

Polymers ◽  
2021 ◽  
Vol 13 (18) ◽  
pp. 3193
Author(s):  
Sylva Holešová ◽  
Karla Čech Čech Barabaszová ◽  
Marianna Hundáková ◽  
Michaela Ščuková ◽  
Kamila Hrabovská ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Surface Properties ◽  
Food Packaging ◽  
Synergistic Effects ◽  
Nanocomposite Films ◽  
Mechanical Resistance ◽  
X Ray Diffraction ◽  
Microbial Strains ◽  
And Storage ◽  
Structure Properties

Infection with pathogenic microorganisms is of great concern in many areas, especially in healthcare, but also in food packaging and storage, or in water purification systems. Antimicrobial polymer nanocomposites have gained great popularity in these areas. Therefore, this study focused on new approaches to develop thin antimicrobial films based on biodegradable polycaprolactone (PCL) with clay mineral natural vermiculite as a carrier for antimicrobial compounds, where the active organic antimicrobial component is antifungal ciclopirox olamine (CPX). For possible synergistic effects, a sample in combination with the inorganic antimicrobial active ingredient zinc oxide was also prepared. The structures of all the prepared samples were studied by X-ray diffraction, FTIR analysis and, predominantly, by SEM. The very different structure properties of the prepared nanofillers had a fundamental influence on the final structural arrangement of thin PCL nanocomposite films as well as on their mechanical, thermal, and surface properties. As sample PCL/ZnOVER_CPX possessed the best results for antimicrobial activity against examined microbial strains, the synergic effect of CPX and ZnO combination on antimicrobial activity was proved, but on the other hand, its mechanical resistance was the lowest.

scholarly journals Increased Resistance of Contact Lens-Related Bacterial Biofilms to Antimicrobial Activity of Soft Contact Lens Care Solutions

Cornea ◽  
2009 ◽  
Vol 28 (8) ◽  
pp. 918-926 ◽  
Author(s):  
Loretta B Szczotka-Flynn ◽  
Yoshifumi Imamura ◽  
Jyotsna Chandra ◽  
Changping Yu ◽  
Pranab K Mukherjee ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Contact Lens ◽  
Bacterial Biofilms ◽  
Soft Contact ◽  
Soft Contact Lens

scholarly journals DEJAVNIKI TVEGANJA ZA ZAPLETE PRI UPORABI KONTAKTNIH LEČ

2016 ◽  
Vol 85 (2) ◽  
Author(s):  
Mirna Štabuc Šilih
Keyword(s):  
Visual Acuity ◽  
Medical History ◽  
Contact Lens ◽  
Contact Lenses ◽  
Living Environment ◽  
Inappropriate Use ◽  
Everyday Work ◽  
And Storage

Contact lenses are freely available without professional examination and fitting, as well as without adequate introduction and instructions on handling, disinfection and storage. Contact lenses may have physiological, metabolic and anatomic effect on the eyes, which is manifested both on the eyelids, lacrimal film, conjunctiva and in all layers of the cornea - the epithelium, stroma and the endothelium. We can see these complications too often at our everyday work. The purpose of this paper is to draw attention to the potential complications due to inappropriate use of contact lenses.Contact lenses are otherwise a safe and effective method of correcting visual acuity; they are also useful for therapeutic purposes. Complications related to contact lenses wear could be largely avoided with an appropriate professional approach and the compliance of contact lens wearers. It is important to discuss with patients their medical history, their desires and expectations, their working and living environment; followed by professional examination of patients and appropriate fitting of contact lenses. Patients should receive detailed oral and written instructions and be subscribed to regular checks. But unfortunately, all this is not enough, if patients do not comply every day with given instructions.

Improving enzyme activity, thermostability and storage stability of β-1,3-1,4-glucanase with poly-γ-glutamic acid produced by Bacillus sp. SJ-10

2021 ◽  
Vol 143 ◽  
pp. 109703
Author(s):  
Won Je Jang ◽  
Ga Hye Lee ◽  
Jong Min Lee ◽  
Tae-Yong Kim ◽  
Mi-Hyun Jeon ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Glutamic Acid ◽  
Storage Stability ◽  
Bacillus Sp ◽  
And Storage

Evaluation of Stabilized Chlorine Dioxide in Terms of Antimicrobial Activity and Dentin Bond Strength

2021 ◽  
Vol 24 ◽  
Author(s):  
Tugba Serin Kalay ◽  
Yakup Kara ◽  
Sengul alpay Karaoglu ◽  
Sevgi Kolaylı
Keyword(s):  
Antimicrobial Activity ◽  
Bond Strength ◽  
Chlorine Dioxide ◽  
Antimicrobial Agents ◽  
Failure Modes ◽  
Testing Machine ◽  
Control Group ◽  
Limited Information ◽  
Significant Difference ◽  
Oral Pathogens

Background: Antimicrobial agents are recommended for disinfection of the cavity following mechanical dental caries removal prior to application of restorative material. There is limited information about stabilized chlorine dioxide (ClO2) as a cavity disinfectant. Objective: The objective of this study is to determine the antimicrobial activity and effect on dentin bond strength of ClO2 compared to chlorhexidine digluconate (CHX), sodium hypochlorite (NaOCl) and ethanolic propolis extract (EPE). Methods: Antimicrobial activities of agents against oral pathogens (Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans, Lactobacillus acidophilus, Lactobacillus casei, Candida albicans, and Saccharomyces cerevisiae) and analyses of EPE were examined. Seventy-five mandibular third molars were sectioned, prepared and divided into five subgroups (n=15/group). Cavity disinfectants (2% CHX, 2.5% NaOCl, 30% EPE, 0.3% ClO2) were applied to etched dentin prior to adhesive and composite build-up. Shear bond strength (SBS) was evaluated with a universal testing machine at a crosshead speed of 0.5 mm/min. The SBS data were analyzed with one-way analysis of variance (ANOVA) and Tukey’s post-hoc test (p <0.05). The failure modes were evaluated with a stereomicroscope. Results: It was determined that the compared disinfectants were showed different inhibition zone values against oral pathogens. ClO2 exhibited the highest antimicrobial activity, followed by CHX, NaOCI and EPE, respectively. No statistically significant difference was observed in the SBS values between the disinfectant treated groups and control group. The failure modes were predominantly mixed. Conclusion: The use of 0.3% stabilized ClO2 as a cavity disinfectant agent exhibited high antimicrobial activity against oral pathogens and no adverse effects on SBS to etched dentin.

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