scholarly journals Degradation Behavior In Vitro of Carbon Nanotubes (CNTs)/Poly(lactic acid) (PLA) Composite Suture

Polymers ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 1015 ◽  
Author(s):  
Shuqiang Liu ◽  
Gaihong Wu ◽  
Xiaogang Chen ◽  
Xiaofang Zhang ◽  
Juanjuan Yu ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Lactic Acid ◽  
Chemical Structure ◽  
Surface Defects ◽  
Healing Time ◽  
Poly Lactic Acid ◽  
Different Types ◽  
Time Required ◽  
Valid Time

Poly(lactic acid) (PLA) suture can be absorbed by the human body, and so have wide applications in modern surgery operations. The degradation period of PLA suture is expected to meet with the healing time of different types of wounds. In order to control the degradation period of the PLA suture, the carbon nanotubes (CNTs) were composited with PLA suture, and the degradation experiment in vitro was performed on sutures. The structure and properties of sutures during degradation, such as surface morphology, breaking strength, elongation, mass and chemical structure, were tracked and analyzed. The results indicated that the degradation brought about surface defects and resulted in 13.5 weeks for the strength valid time of the original PLA suture. By contrast, the strength valid time of the CNTs/PLA suture was increased to 26.6 weeks. Whilst the toughness of both the pure PLA and CNTs/PLA sutures decreased rapidly and almost disappeared after 3 to 4 weeks of degradation. The mass loss demonstrated that the time required for complete degradation of the two sutures was obviously different, the pure PLA suture 49 weeks, while CNTs/PLA sutures 63 to 73 weeks. The research proved that CNTs delayed PLA degradation and prolonged its strength valid time in degradation.

Three-dimensional wet-electrospun poly(lactic acid)/multi-wall carbon nanotubes scaffold induces differentiation of human menstrual blood-derived stem cells into germ-like cells

2017 ◽  
Vol 32 (3) ◽  
pp. 373-383 ◽  
Author(s):  
Hossein Eyni ◽  
Sadegh Ghorbani ◽  
Reza Shirazi ◽  
Leila Salari Asl ◽  
Shahram P Beiranvand ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Stem Cells ◽  
Lactic Acid ◽  
Germ Cells ◽  
Three Dimensional ◽  
Menstrual Blood ◽  
Poly Lactic Acid ◽  
Multi Wall Carbon Nanotubes ◽  
Proliferation And Differentiation

Infertility caused by the disruption or absence of germ cells is a major and largely incurable medical problem. Germ cells (i.e., sperm or egg) play a key role in the transmission of genetic and epigenetic information across generations. Generation of gametes derived in vitro from stem cells hold promising prospects which could potentially help infertile men and women. Menstrual blood-derived stem cells are a unique stem cell source. Evidence suggests that menstrual blood-derived stem cells exhibit a multi-lineage potential and have attracted extensive attention in regenerative medicine. To maintain the three-dimensional structure of natural extra cellular matrices in vitro, scaffolds can do this favor and mimic a microenvironment for cell proliferation and differentiation. According to previous studies, poly(lactic acid) and multi-wall carbon nanotubes have been introduced as novel and promising biomaterials for the proliferation and differentiation of stem cells. Some cell types have been successfully grown on a matrix containing carbon nanotubes in tissue engineering but there is no report for this material to support stem cells differentiation into germ cells lineage. This study designed a 3D wet-electrospun poly(lactic acid) and poly(lactic acid)/multi-wall carbon nanotubes composite scaffold to compare infiltration, proliferation, and differentiation potential of menstrual blood-derived stem cells toward germ cell lineage with 2D culture. Our primary data revealed that the fabricated scaffold has mechanical and biological suitable qualities for supporting and attachments of stem cells. The differentiated menstrual blood-derived stem cells tracking in scaffolds using scanning electron microscopy confirmed cell attachment, aggregation, and distribution on the porous scaffold. Based on the differentiation assay by RT-PCR analysis, stem cells and germ-like cells markers were expressed in 3D groups as well as 2D one. It seems that poly(lactic acid)/multi-wall carbon nanotubes scaffold-seeded menstrual blood-derived stem cells could be viewed as a novel, safe, and accessible construct for these cells, as they enhance germ-like generation from menstrual blood-derived stem cells.

scholarly journals In Vitro and In Vivo Biosafety Analysis of Resorbable Polyglycolic Acid-Polylactic Acid Block Copolymer Composites for Spinal Fixation

Polymers ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 29
Author(s):  
Seung Kyun Yoon ◽  
Jin Ho Yang ◽  
Hyun Tae Lim ◽  
Young-Wook Chang ◽  
Muhammad Ayyoob ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Animal Experiments ◽  
Polymeric Materials ◽  
Polyglycolic Acid ◽  
Poly Lactic Acid ◽  
Skin Sensitization ◽  
Spinal Fixation ◽  
In Vivo Degradation

Herein, spinal fixation implants were constructed using degradable polymeric materials such as PGA–PLA block copolymers (poly(glycolic acid-b-lactic acid)). These materials were reinforced by blending with HA-g-PLA (hydroxyapatite-graft-poly lactic acid) and PGA fiber before being tested to confirm its biocompatibility via in vitro (MTT assay) and in vivo animal experiments (i.e., skin sensitization, intradermal intracutaneous reaction, and in vivo degradation tests). Every specimen exhibited suitable biocompatibility and biodegradability for use as resorbable spinal fixation materials.

Shear Flow and Carbon Nanotubes Synergistically Induced Nonisothermal Crystallization of Poly(lactic acid) and Its Application in Injection Molding

2012 ◽  
Vol 13 (11) ◽  
pp. 3858-3867 ◽  
Author(s):  
Hu Tang ◽  
Jing-Bin Chen ◽  
Yan Wang ◽  
Jia-Zhuang Xu ◽  
Benjamin S. Hsiao ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Lactic Acid ◽  
Shear Flow ◽  
Injection Molding ◽  
Poly Lactic Acid ◽  
Nonisothermal Crystallization

Properties of poly(lactic acid)/montmorillonite/carbon nanotubes nanocomposites: determination of percolation threshold

2021 ◽  
Author(s):  
Olawale Monsur Sanusi ◽  
Abdelkibir Benelfellah ◽  
Lazaros Papadopoulos ◽  
Zoe Terzopoulou ◽  
Dimitrios N. Bikiaris ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Lactic Acid ◽  
Percolation Threshold ◽  
Poly Lactic Acid

scholarly journals Lactic Acid Bacteria Co-Encapsulated with Lactobionic Acid: Probiotic Viability during In Vitro Digestion

2021 ◽  
Vol 11 (23) ◽  
pp. 11404
Author(s):  
Sara Sáez-Orviz ◽  
Francesca Passannanti ◽  
Marianna Gallo ◽  
Rosa Colucci Cante ◽  
Federica Nigro ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Saline Solution ◽  
Lactic Acid Production ◽  
In Vitro Digestion ◽  
Lactobionic Acid ◽  
Different Types ◽  
Stability Time ◽  
Over Time ◽  
Improve Health

Synbiotic products are a type of functional food with great potential due to consumer interest in foods that improve health and/or reduce the risk of certain diseases. In this study, synbiotic macrocapsules were developed using Lactobacillus paracasei CBA L74 as probiotic and lactobionic acid (LBA) as prebiotic. Firstly, the probiotic was proven to be able to use LBA as the only substrate source checking their growth and lactic acid production. Then, four different types of capsules were produced using sodium alginate as matrix and different hardener solutions (CaCl2 and chitosan). The macrocapsules were characterised regarding their strength, and the best performing ones were used for further analysis. In order to obtain a synbiotic capsule characterized by a longer stability time due to low water activity, the capsules were dried using freeze and thermal drying. Successively, to revitalize the microorganisms, the capsules were rehydrated in two different media (saline solution and yogurt), subjected to simulated in vitro digestion tests and visually characterised. In addition, their viability over time was assessed. As a result, L. paracasei was able to grow using LBA as the only source of carbon with better production of lactic acid for prolonged periods. The more resistant freeze- and thermal-dried capsules showed differences in rehydration kinetics, and visual changes were also observed. In simulated in vitro digestion tests, capsules rehydrated in yogurt showed the best results in terms of survival. Regarding their viability over time, the importance of the use of chitosan was noted.

scholarly journals Drug Release from Electrospun Poly(Lactic Acid) Membranes and Their Cell Viability in Vitro Test

2012 ◽  
Vol 44 ◽  
pp. 866-868 ◽  
Author(s):  
A.P.S. Immich ◽  
M. Lis ◽  
L.H. Catalani ◽  
R.L. Boemo ◽  
J.A. Tornero
Keyword(s):  
Lactic Acid ◽  
Drug Release ◽  
Cell Viability ◽  
Poly Lactic Acid ◽  
In Vitro Test ◽  
Vitro Test

In vitro degradation of dry-jet-wet spun poly(lactic acid) monofilament and knitted scaffold

2006 ◽  
Vol 103 (3) ◽  
pp. 2006-2012 ◽  
Author(s):  
Bhuvanesh Gupta ◽  
Nilesh Revagade ◽  
Jöns Hilborn
Keyword(s):  
Lactic Acid ◽  
Poly Lactic Acid ◽  
In Vitro Degradation
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