scholarly journals Therapeutic Effect and Metabolic Mechanism of A Selenium-Polysaccharide from Ziyang Green Tea on Chronic Fatigue Syndrome

Polymers ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 1269 ◽  
Author(s):  
Changzhuan Shao ◽  
Jing Song ◽  
Shanguang Zhao ◽  
Hongke Jiang ◽  
Baoping Wang ◽  
...  
Keyword(s):  
Therapeutic Effect ◽  
Green Tea ◽  
Metabolic Pathways ◽  
Steroid Hormone ◽  
Chronic Fatigue ◽  
Gas Chromatography Mass Spectrometry ◽  
Urine Metabolites ◽  
Steroid Hormone Biosynthesis ◽  
Hormone Biosynthesis ◽  
Metabolic Mechanism

Ziyang green tea was considered a medicine food homology plant to improve chronic fatigue Ssyndrome (CFS) in China. The aim of this research was to study the therapeutic effect of selenium-polysaccharides (Se-TP) from Ziyang green tea on CFS and explore its metabolic mechanism. A CFS-rats model was established in the present research and Se-TP was administrated to evaluate the therapeutic effect on CFS. Some serum metabolites including blood urea nitrogen (BUN), blood lactate acid (BLA), corticosterone (CORT), and aldosterone (ALD) were checked. Urine metabolites were analyzed via gas chromatography-mass spectrometry (GC-MS). Multivariate statistical analysis was also used to check the data. The results selected biomarkers that were entered into the MetPA database to analyze their corresponding metabolic pathways. The results demonstrated that Se-TP markedly improved the level of BUN and CORT in CFS rats. A total of eight differential metabolites were detected in GC-MS analysis, which were benzoic acid, itaconic acid, glutaric acid, 4-acetamidobutyric acid, creatine, 2-hydroxy-3-isopropylbutanedioic acid, l-dopa, and 21-hydroxypregnenolone. These differential metabolites were entered into the MetPA database to search for the corresponding metabolic pathways and three related metabolic pathways were screened out. The first pathway was steroid hormone biosynthesis. The second was tyrosine metabolism, and the third was arginine-proline metabolism. The 21-hydroxypregnenolone level of rats in the CFS group markedly increased after the Se-TP administration. In conclusion, Se-TP treatments on CFS rats improved their condition. Its metabolic mechanism was closely related to that which regulates the steroid hormone biosynthesis.

scholarly journals Detection of Urine Metabolites in a Rat Model of Chronic Fatigue Syndrome before and after Exercise

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Changzhuan Shao ◽  
Yiming Ren ◽  
Zinan Wang ◽  
Chenzhe Kang ◽  
Hongke Jiang ◽  
...  
Keyword(s):  
Chronic Fatigue Syndrome ◽  
Rat Model ◽  
Steroid Hormone ◽  
Chronic Fatigue ◽  
Sphingolipid Metabolism ◽  
Urine Metabolites ◽  
Steroid Hormone Biosynthesis ◽  
Fatigue Syndrome ◽  
Before And After ◽  
Hormone Biosynthesis

Purpose. The aim of the present study was to elucidate the metabolic mechanisms associated with chronic fatigue syndrome (CFS) via an analysis of urine metabolites prior to and following exercise in a rat model. Methods. A rat model of CFS was established using restraint-stress, forced exercise, and crowded and noisy environments over a period of 4 weeks. Behavioral experiments were conducted in order to evaluate the model. Urine metabolites were analyzed via gas chromatography-mass spectrometry (GC-MS) in combination with multivariate statistical analysis before and after exercise. Results. A total of 20 metabolites were detected in CFS rats before and after exercise. Three metabolic pathways (TCA cycle; alanine, aspartate, and glutamate metabolism; steroid hormone biosynthesis) were significantly impacted before and after exercise, while sphingolipid metabolism alone exhibited significant alterations after exercise only. Conclusion. In addition to metabolic disturbances involving some energy substances, alterations in steroid hormone biosynthesis and sphingolipid metabolism were detected in CFS rats. Sphingosine and 21-hydroxypregnenolone may be key biomarkers of CFS, potentially offering evidence in support of immune dysfunction and hypothalamic-pituitary-adrenal (HPA) axis hypoactivity in patients with CFS.

scholarly journals Untargeted Metabolomics and Steroid Signatures in Urine of Male Pattern Baldness Patients after Finasteride Treatment for a Year

Metabolites ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 131 ◽  
Author(s):  
Yu Ra Lee ◽  
Eunju Im ◽  
Haksoon Kim ◽  
Bark Lynn Lew ◽  
Woo-Young Sim ◽  
...  
Keyword(s):  
Steroid Hormone ◽  
Normal Subjects ◽  
Healthy Controls ◽  
Male Pattern Baldness ◽  
Steroid Hormone Biosynthesis ◽  
Physiological Alterations ◽  
Urinary Androgens ◽  
Hormone Biosynthesis ◽  
One Year ◽  
Male Pattern

Male pattern baldness (MPB) has been associated with dihydrotestosterone (DHT) expression. Finasteride treats MPB by inhibiting 5-alpha reductase and blocking DHT production. In this study, we aimed to identify metabolic differences in urinary metabolomics profiles between MPB patients after a one-year treatment with finasteride and healthy controls. Untargeted and targeted metabolomics profiling was performed using liquid chromatography-mass spectrometry (LC-MS). We hypothesized that there would be changes in overall metabolite concentrations, especially steroids, in the urine of hair loss patients treated with finasteride and normal subjects. Untargeted analysis indicated differences in steroid hormone biosynthesis. Therefore, we conducted targeted profiling for steroid hormone biosynthesis to identify potential biomarkers, especially androgens and estrogens. Our study confirmed the differences in the concentration of urinary androgens and estrogens between healthy controls and MPB patients. Moreover, the effect of finasteride was confirmed by the DHT/T ratio in urine samples of MPB patients. Our metabolomics approach provided insight into the physiological alterations in MPB patients who have been treated with finasteride for a year and provided evidence for the association of finasteride and estrogen levels. Through a targeted approach, our results suggest that urinary estrogens must be studied in relation to MPB and post-finasteride syndrome.

scholarly journals Role of AMP-Activated Protein Kinase on Steroid Hormone Biosynthesis in Adrenal NCI-H295R Cells

PLoS ONE ◽  
2012 ◽  
Vol 7 (1) ◽  
pp. e30956 ◽  
Author(s):  
Andrea Hirsch ◽  
Dagmar Hahn ◽  
Petra Kempná ◽  
Gaby Hofer ◽  
Primus E. Mullis ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Steroid Hormone ◽  
Steroid Hormone Biosynthesis ◽  
H295r Cells ◽  
Hormone Biosynthesis ◽  
Amp Activated Protein Kinase

REGULATION OF STEROID HORMONE BIOSYNTHESIS IN R2C AND MA-10 LEYDIG TUMOR CELLS: ROLE OF THE CHOLESTEROL TRANSFER PROTEINS StAR AND PBR

2002 ◽  
Vol 28 (4) ◽  
pp. 387-394 ◽  
Author(s):  
Rekha M. Rao ◽  
Youngah Jo ◽  
Michelle Babb-Tarbox ◽  
Peter J. Syapin ◽  
Douglas M. Stocco
Keyword(s):  
Tumor Cells ◽  
Steroid Hormone ◽  
Steroid Hormone Biosynthesis ◽  
Leydig Tumor Cells ◽  
Hormone Biosynthesis
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