scholarly journals Plasma Activation as a Powerful Tool for Selective Modification of Cellulose Fibers towards Biomedical Applications

Plasma ◽  
2020 ◽  
Vol 3 (4) ◽  
pp. 196-203
Author(s):  
Olivia Mauger ◽  
Sophia Westphal ◽  
Stefanie Klöpzig ◽  
Anne Krüger-Genge ◽  
Werner Müller ◽  
...  
Keyword(s):  
Photoelectron Spectroscopy ◽  
Bacterial Adhesion ◽  
Biomedical Applications ◽  
Cellulose Fibers ◽  
Plasma Technology ◽  
X Ray ◽  
Cellulose Modification ◽  
Plasma Activation ◽  
Low Pressure Plasma ◽  
Pre Treatment

Cellulosic substrates are known for their biocompatibility, non-cytotoxicity, hypoallergenicity and sterilizability. It is therefore desirable to have a bundle of methods to equip them with tailored properties such as affinity profiles for various applications. In the case of highly swelling materials such as cellulose sponges, “dry” functionalization using plasma activation is the method of choice. The purpose of the study was to adapt low-pressure plasma technology for targeted cellulose modification. Using plasma (pre-) treatment combined with gaseous reactants like O2, ethylene oxide or silane, three different cellulose modifications were obtained and characterized by X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR). Swelling measurements and bacterial adhesion tests revealed distinctive material properties compared to educt. The development of these non-aqueous methods demonstrated an effective procedural route towards modified cellulosic materials for usage in wound dressing, micro patterned assays or bacterial filtration.

scholarly journals α-Cellulose Fibers of Paper-Waste Origin Surface-Modified with Fe3O4 and Thiolated-Chitosan for Efficacious Immobilization of Laccase

Polymers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 581
Author(s):  
Gajanan S. Ghodake ◽  
Surendra K. Shinde ◽  
Ganesh D. Saratale ◽  
Rijuta G. Saratale ◽  
Min Kim ◽  
...  
Keyword(s):  
Enzyme Immobilization ◽  
Photoelectron Spectroscopy ◽  
Cellulose Fibers ◽  
Relative Activity ◽  
Significant Activity ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron ◽  
Laccase Immobilization ◽  
Surface Modified

The utilization of waste-paper-biomass for extraction of important α-cellulose biopolymer, and modification of extracted α-cellulose for application in enzyme immobilization can be extremely vital for green circular bio-economy. Thus, in this study, α-cellulose fibers were super-magnetized (Fe3O4), grafted with chitosan (CTNs), and thiol (-SH) modified for laccase immobilization. The developed material was characterized by high-resolution transmission electron microscopy (HR-TEM), HR-TEM energy dispersive X-ray spectroscopy (HR-TEM-EDS), X-ray diffraction (XRD), vibrating sample magnetometer (VSM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FT-IR) analyses. Laccase immobilized on α-Cellulose-Fe3O4-CTNs (α-Cellulose-Fe3O4-CTNs-Laccase) gave significant activity recovery (99.16%) and laccase loading potential (169.36 mg/g). The α-Cellulose-Fe3O4-CTNs-Laccase displayed excellent stabilities for temperature, pH, and storage time. The α-Cellulose-Fe3O4-CTNs-Laccase applied in repeated cycles shown remarkable consistency of activity retention for 10 cycles. After the 10th cycle, α-Cellulose-Fe3O4-CTNs possessed 80.65% relative activity. Furthermore, α-Cellulose-Fe3O4-CTNs-Laccase shown excellent degradation of pharmaceutical contaminant sulfamethoxazole (SMX). The SMX degradation by α-Cellulose-Fe3O4-CTNs-Laccase was found optimum at incubation time (20 h), pH (3), temperatures (30 °C), and shaking conditions (200 rpm). Finally, α-Cellulose-Fe3O4-CTNs-Laccase gave repeated degradation of SMX. Thus, this study presents a novel, waste-derived, highly capable, and super-magnetic nanocomposite for enzyme immobilization applications.

Physical Chemistry of the Interface between Attached Micro-Organisms and Their Support

1990 ◽  
Vol 22 (1-2) ◽  
pp. 1-16 ◽  
Author(s):  
P. G. Rouxhet ◽  
N. Mozes
Keyword(s):  
Photoelectron Spectroscopy ◽  
Bacterial Adhesion ◽  
Surface Composition ◽  
Chemical Properties ◽  
Dlvo Theory ◽  
Support Surface ◽  
X Ray ◽  
Polymer Molecules ◽  
Physico Chemical ◽  
Micro Organisms

The thermodynamic approach of adhesion and DLVO theory are complementary to predict initial bacterial adhesion; the interplay between short- and long-range forces, respectively, may be due to surface roughness. Due to the influence of electrical double layer interactions, adhesion can be promoted by treatments leading to modification of the cell or support surface properties. Adhesion is influenced by cell-cell interactions, by the cpresence of polymer molecules on the surface and by the composition of the medium. X-ray photoelectron spectroscopy can be applied to determine the elemental composition of the surface of microorganisms; some information on the chemical functions can also be obtained. The surface composition is related to physico-chemical properties which play a determining role in adhesion and flocculation, in particular the hydrophobicity and the zeta potential.

The Study of Immobilization Thermal-Sensitive Hydrogel onto ePTFE Film Use the Cold Plasma and Photo-Grafting Technique

2006 ◽  
Vol 15-17 ◽  
pp. 187-192 ◽  
Author(s):  
Ko Shao Chen ◽  
Su Chen Chen ◽  
Yi Chun Yeh ◽  
Wei Cheng Lien ◽  
Hong Ru Lin ◽  
...  
Keyword(s):  
Contact Angle ◽  
Acetic Acid ◽  
Water Contact Angle ◽  
Photoelectron Spectroscopy ◽  
Cold Plasma ◽  
Plasma Deposition ◽  
Plasma Technology ◽  
Water Contact ◽  
X Ray ◽  
Grafting Technique

Expanded polytetrafluoroethylene (ePTFE) is a bioinert material. To improve the ePTFE film biocompatibility, the cold plasma technology was used with acetic acid as monomer to deposit onto ePTFE film and then (N-isopropylacrylamide) was grafted onto the surface by photo-grafting. The characteristics of the surface were evaluated with X-ray photoelectron spectroscopy (XPS), FTIR and water contact angle. It was found that the contact angle of water on the untreated ePTFE significantly decrease from125° to 72° after ePTFE film being treated with acetic acid plasma deposition treatment. Due to the hydrophilicity of poly(N-isopropylacrylamide), the contact angle of water on the ePTFE-g-NIPAAm approached to 0°.

scholarly journals Influence of the Double Bond LDH Clay on the Exfoliation / Intercalation Mechanism of Polyacrylamide Nanocomposite Hydrogels

2018 ◽  
Vol 55 (3) ◽  
pp. 263-268
Author(s):  
Ionut Cristian Radu ◽  
Eugeniu Vasile ◽  
Celina Maria Damian ◽  
Horia Iovu ◽  
Paul Octavian Stanescu ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Photoelectron Spectroscopy ◽  
Biomedical Applications ◽  
X Ray Diffraction ◽  
X Ray ◽  
Nanocomposite Hydrogels ◽  
Transmission Electron ◽  
Double Hydroxides ◽  
Classical Cross ◽  
Chemical Cross Linking

The paper focuses on the obtaining of novel nanocomposite hydrogels based on polyacrylamide and layered double hydroxides (LDHs) modified with double bonds. The modification of LDH clay was investigated by FTIR, X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) analyses. Mechanical properties of the nanocomposite hydrogels were employed by compression and rheological measurements. The formation of exfoliated and intercalated structures was evidenced in transmission electron microscopy (TEM). Chemical cross-linking of hydrogels using both classical cross-linker and modified clay was an efficient method to improve the mechanical properties of novel nanocomposite hydrogels. These hydrogels with improved mechanical properties could be further tested for biomedical applications such as tissue engineering.

scholarly journals Structure and Properties of - Nanocomposite Films for Biomedical Applications

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Lin Zhang ◽  
Zhi-Hua Sun ◽  
Feng-Mei Yu ◽  
Hong-Bin Chen
Keyword(s):  
Photoelectron Spectroscopy ◽  
Biomedical Applications ◽  
Blood Compatibility ◽  
Rf Sputtering ◽  
Nanocomposite Films ◽  
Optical Absorption Spectroscopy ◽  
X Ray ◽  
Type Semiconductor ◽  
Uv Visible ◽  
Semiconductor Properties

The hemocompatibility of La2O3-doped TiO2films with different concentration prepared by radio frequency (RF) sputtering was studied. The microstructures and blood compatibility of TiO2films were investigated by scan electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and UV-visible optical absorption spectroscopy, respectively. With the increasing of the La2O3concentrations, the TiO2films become smooth, and the grain size becomes smaller. Meanwhile, the band gap of the samples increases from 2.85 to 3.3 eV with increasing of the La2O3content in TiO2films from 0 to 3.64%. La2O3-doped TiO2films exhibit n-type semiconductor properties due to the existence of Ti2+and Ti3+. The mechanism of hemocompatibility of TiO2film doped with La2O3was analyzed and discussed.

scholarly journals Ag Nanoparticle-Decorated Oxide Coatings Formed via Plasma Electrolytic Oxidation on ZrNb Alloy

Materials ◽  
2019 ◽  
Vol 12 (22) ◽  
pp. 3742 ◽  
Author(s):  
Oleksandr Oleshko ◽  
Volodymyr Deineka V ◽  
Yevgeniia Husak ◽  
Viktoriia Korniienko ◽  
Oleg Mishchenko ◽  
...  
Keyword(s):  
Oxide Layer ◽  
Photoelectron Spectroscopy ◽  
Bacterial Adhesion ◽  
Plasma Electrolytic Oxidation ◽  
Infectious Complications ◽  
Osteoblast Cell ◽  
Effective Prevention ◽  
X Ray ◽  
Electrolytic Oxidation ◽  
Plasma Electrolytic

Plasma electrolytic oxidation (PEO) can provide an ideal surface for osteogenic cell attachment and proliferation with further successful osteointegration. However, the same surface is attractive for bacteria due to similar mechanisms of adhesion in prokaryotic and eukaryotic cells. This issue requires the application of additional surface treatments for effective prevention of postoperative infectious complications. In the present work, ZrNb alloy was treated in a Ca-P solution with Ag nanoparticles (AgNPs) for the development of a new oxide layer that hosted osteogenic cells and prevented bacterial adhesion. For the PEO, 0.5 M Ca(H2PO2)2 solution with 264 mg L−1 of round-shaped AgNPs was used. Scanning electron microscopy with energy-dispersive x-ray and x-ray photoelectron spectroscopy were used for morphology and chemical analysis of the obtained samples; the SBF immersion test, bacteria adhesion test, and osteoblast cell culture were used for biological investigation. PEO in a Ca-P bath with AgNPs provides the formation of a mesoporous oxide layer that supports osteoblast cell adhesion and proliferation. Additionally, the obtained surface with incorporated Ag prevents bacterial adhesion in the first 6 h after immersion in a pathogen suspension, which can be an effective approach to prevent infectious complications after implantation.

Material and Interface Characterization of Cu99Ti1 Thin Films Metallized on Polyimide

1997 ◽  
Vol 476 ◽  
Author(s):  
E. Kondoh ◽  
T.P. Nguyen
Keyword(s):  
Photoelectron Spectroscopy ◽  
Analysis Data ◽  
Alloy Element ◽  
Composition Analysis ◽  
Copper Films ◽  
X Ray ◽  
Interface Characterization ◽  
Drastic Increase ◽  
Pre Treatment

AbstractCopper films with a small amount of an alloy element (1 wt % Ti) were metallized on polyimide. Plasma pre-treatment of the polyimide surface and post-metallization annealing were used to modify the interface. Interfaces and metal film layers were investigated; a drastic increase in adhesion strength, the suppression of Cu diffusion into polyimide, and the improvement of (111) texture were found. Composition analysis data taken from the interface indicated the accretion of nitrogen at the interface. The formation of Ti-related chemical bonds, suggested by X-ray photoelectron spectroscopy, can explain the above-mentioned experimental results.

An Experimental Study on the Effect of Aqueous Hypophosphite Pre-Treatment Used on an Aluminium Alloy Substrate before Electroless Nickel Plating

2010 ◽  
Vol 659 ◽  
pp. 103-108 ◽  
Author(s):  
Georgina Szirmai ◽  
József Tóth ◽  
Tamas I. Török ◽  
Norbert Hegman
Keyword(s):  
Electron Microscopy ◽  
Photoelectron Spectroscopy ◽  
Electroless Nickel ◽  
Sodium Hypophosphite ◽  
Treatment Technique ◽  
Electroless Nickel Plating ◽  
Nickel Plating ◽  
Plating Bath ◽  
X Ray ◽  
Pre Treatment

A new surface pre-treatment procedure has been developed in one of our laboratories [1] for electroless nickel (EN) plating, which appears to be an effective and environmentally benign treatment for the following deposition of a sound and high quality surface nickel coating with good adhesion. For that purpose, the aluminium substrate was immersed in a mildly acidic solution (lactic acid) of sodium hypophosphite in order to modify the passive surface and make it suitable for the reductive chemical precipitation of the nickel-phosphorus nuclei from the electroless nickel plating bath. During this novel pre-treatment technique, the surface adsorption of the hypophosphite anions play an important role therefore several advanced surface testing and analytical techniques (Scanning Electron Microscopy-SEM, Transmission Electron Microscopy-TEM, Energy Dispersive X-ray Spectrometry-EDX, X-ray Photoelectron Spectroscopy-XPS were applied in order to monitor and characterize the surface reactions and adsorption phenomena taking place during the pre-treatment. The Al excited XPS (studying P 2p, O 1s, C 1s, Al 2p, Ni 2p photoelectron lines) proved to be one of the most powerful technique in the identification of the chemical species formed and present on the surfaces examined in this study.

Hydroxyapatite Coatings Produced by Right Angle Magnetron Sputtering for Biomedical Applications

2007 ◽  
Vol 1008 ◽  
Author(s):  
Zhendong Hong ◽  
Alexandre Mello ◽  
Tomohiko Yoshida ◽  
Lan Luan ◽  
Paula H. Stern ◽  
...  
Keyword(s):  
Magnetron Sputtering ◽  
Photoelectron Spectroscopy ◽  
Biomedical Applications ◽  
Titanium Substrate ◽  
Degree Of Crystallinity ◽  
X Ray Diffraction ◽  
X Ray ◽  
Force Microscopy ◽  
Atomic Force ◽  
Hydroxyapatite Coatings

AbstractHydroxyapatite coatings have been widely recognized for their biocompatibility and utility in promoting biointegration of implants in both osseous and soft tissue. Conventional sputtering techniques have shown some advantages over the commercially available plasma spraying method; however, the as-sputtered coatings are usually non-stoichiometric and amorphous which can cause some serious problems such as poor adhesion and excessive coating dissolution rate. A versatile right-angle radio frequency magnetron sputtering (RAMS) approach has been developed to deposit HA coatings on various substrates at low power levels. Using this alternative magnetron geometry, as-sputtered HA coatings are nearly stoichiometric, highly crystalline, and strongly bound to the substrate, as evidenced by analyses using x-ray diffraction (XRD), atomic force microscopy (AFM), x-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FTIR). In particular, coatings deposited on oriented substrates show a polycrystalline XRD pattern but with some strongly preferred orientations, indicating that HA crystallization is sensitive to the nature of the substrate. Post deposition heat treatment under high temperature does not result in a marked improvement in the degree of crystallinity of the coatings. To study the biocompatibility of these coatings, murine osteoblast cells were seeded onto various substrates. Cell density counts using fluorescence microscopy show that the best osteoblast proliferation is achieved on an HA RAMS-coated titanium substrate. These experiments demonstrate that RAMS is a promising coating technique for biomedical applications.

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