scholarly journals Strawberry FaNAC2 Enhances Tolerance to Abiotic Stress by Regulating Proline Metabolism

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1417
Author(s):  
Jiahui Liang ◽  
Jing Zheng ◽  
Ze Wu ◽  
Hongqing Wang
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Abiotic Stress ◽  
Plant Responses ◽  
Drought Treatment ◽  
Abscisic Acid Biosynthesis ◽  
Positive Role ◽  
Transgenic Lines ◽  
Response To Stress ◽  
Simulated Drought

The quality and yields of strawberry plants are seriously affected by abiotic stress every year. NAC (NAM, ATAF, CUC) transcription factors are plant-specific, having various functions in plant development and response to stress. In our study, FaNAC2 from strawberry (Fragaria × ananassa, cultivar “Benihoppe”) was isolated and found to be a member of the ATAF sub-family, belonging to the NAC family of transcription factors. FaNAC2 was strongly expressed in the shoot apical meristem and older leaves of strawberries, and was induced by cold, high salinity, and drought stress. To investigate how FaNAC2 functions in plant responses to abiotic stress, transgenic Nicotiana benthamiana plants ectopically overexpressing FaNAC2 were generated. The transgenic plants grew better under salt and cold stress, and, during simulated drought treatment, these transgenic lines not only grew better, but also showed higher seed germination rates than wild-type plants. Gene expression analysis revealed that key genes in proline biosynthesis pathways were up-regulated in FaNAC2 overexpression lines, while its catabolic pathway genes were down-regulated and proline was accumulated more with the overexpression of FaNAC2 after stress treatments. Furthermore, the gene expression of abscisic acid biosynthesis was also promoted. Our results demonstrate that FaNAC2 plays an important positive role in response to different abiotic stresses and may be further utilized to improve the stress tolerance of strawberry plants.

scholarly journals Roles of arabidopsis WRKY18, WRKY40 and WRKY60 transcription factors in plant responses to abscisic acid and abiotic stress

2010 ◽  
Vol 10 (1) ◽  
pp. 281 ◽  
Author(s):  
Han Chen ◽  
Zhibing Lai ◽  
Junwei Shi ◽  
Yong Xiao ◽  
Zhixiang Chen ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Abscisic Acid ◽  
Abiotic Stress ◽  
Plant Responses

scholarly journals Label-free quantitative proteomic analysis of alfalfa in response to microRNA156 under high temperature

2020 ◽  
Author(s):  
Muhammad Arshad ◽  
Alpa Puri ◽  
Aaron J Simkovich ◽  
Justin Renaud ◽  
Margaret Y Gruber ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Heat Stress ◽  
Abiotic Stress ◽  
Stress Response ◽  
Protein Level ◽  
Forage Yield ◽  
Label Free ◽  
Multiple Traits ◽  
Positive Role ◽  
Empty Vector

Abstract Background Abiotic stress, including heat, is one of the major factors that affect alfalfa growth and forage yield. The small RNA, microRNA156 (miR156), regulates multiple traits in alfalfa during abiotic stress. The aim of this study was to explore the role of miR156 in regulating heat response in alfalfa at the protein level. Results In this study, we compared an empty vector control and miR156 overexpressing (miR156OE) alfalfa plants after exposing them to heat stress (40 ° C) for 24 hours. We measured physiological parameters of control and miR156OE plants under heat stress, and collected leaf samples for protein analysis. A higher proline and antioxidant contents were detected in miR156OE plants than in controls under heat stress. Protein samples were analyzed by label-free quantification proteomics. Across all samples, a total of 1878 protein groups were detected. Under heat stress, 45 protein groups in the empty vector plants were significantly altered (P < 0.05; |log 2 FC| > 2). Conversely, 105 protein groups were significantly altered when miR156OE alfalfa was subjected to heat stress, of which 91 were unique to miR156OE plants. The identified protein groups unique to miR156OE plants were related to diverse functions including metabolism, photosynthesis, stress-response and plant defenses. Furthermore, we identified transcription factors in miR156OE plants, which belonged to squamosa promoter binding-like protein, MYB, ethylene responsive factors, AP2 domain, ABA response element binding factor and bZIP families of transcription factors. Conclusions These results suggest a positive role for miR156 in heat stress response in alfalfa. They reveal a miR156-regulated network of mechanisms at the protein level to modulate heat responses in alfalfa.

scholarly journals The Role of Stress-Responsive Transcription Factors in Modulating Abiotic Stress Tolerance in Plants

Agronomy ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 788 ◽  
Author(s):  
Youngdae Yoon ◽  
Deok Hyun Seo ◽  
Hoyoon Shin ◽  
Hui Jin Kim ◽  
Chul Min Kim ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Stress Responses ◽  
Abiotic Stresses ◽  
Crop Production ◽  
Global Climate ◽  
Abiotic Stress Tolerance ◽  
Plant Responses ◽  
Plant Tolerance

Abiotic stresses, such as drought, high temperature, and salinity, affect plant growth and productivity. Furthermore, global climate change may increase the frequency and severity of abiotic stresses, suggesting that development of varieties with improved stress tolerance is critical for future sustainable crop production. Improving stress tolerance requires a detailed understanding of the hormone signaling and transcriptional pathways involved in stress responses. Abscisic acid (ABA) and jasmonic acid (JA) are key stress-response hormones in plants, and some stress-responsive transcription factors such as ABFs and MYCs function as direct components of ABA and JA signaling, playing a pivotal role in plant tolerance to abiotic stress. In addition, extensive studies have identified other stress-responsive transcription factors belonging to the NAC, AP2/ERF, MYB, and WRKY families that mediate plant response and tolerance to abiotic stress. These suggest that transcriptional regulation of stress-responsive genes is an essential step to determine the mechanisms underlying plant stress responses and tolerance to abiotic stress, and that these transcription factors may be important targets for development of crops with enhanced abiotic stress tolerance. In this review, we briefly describe the mechanisms underlying plant abiotic stress responses, focusing on ABA and JA metabolism and signaling pathways. We then summarize the diverse array of transcription factors involved in plant responses to abiotic stress, while noting their potential applications for improvement of stress tolerance.

scholarly journals Transposable elements contribute to activation of maize genes in response to abiotic stress

2014 ◽  
Author(s):  
Irina Makarevitch ◽  
Amanda J Waters ◽  
Patrick T West ◽  
Michelle C Stitzer ◽  
Jeffrey Ross-Ibarra ◽  
...  
Keyword(s):  
Gene Expression ◽  
Abiotic Stress ◽  
Transposable Elements ◽  
Environmental Changes ◽  
Stress Conditions ◽  
Gene Expression Response ◽  
Regulatory Variation ◽  
Ideal System ◽  
Response To Stress ◽  
Regulated Gene Expression

Transposable elements (TEs) account for a large portion of the genome in many eukaryotic species. Despite their reputation as "junk" DNA or genomic parasites deleterious for the host, TEs have complex interactions with host genes and the potential to contribute to regulatory variation in gene expression. It has been hypothesized that TEs and genes they insert near may be transcriptionally activated in response to stress conditions. The maize genome, with many different types of TEs interspersed with genes, provides an ideal system to study the genome-wide influence of TEs on gene regulation. To analyze the magnitude of the TE effect on gene expression response to environmental changes, we profiled gene and TE transcript levels in maize seedlings exposed to a number of abiotic stresses. Many genes exhibit up- or down-regulation in response to these stress conditions. The analysis of TE families inserted within upstream regions of up-regulated genes revealed that between four and nine different TE families are associated with up-regulated gene expression in each of these stress conditions, affecting up to 20% of the genes up-regulated in response to abiotic stress and as many as 33% of genes that are only expressed in response to stress. Expression of many of these same TE families also responds to the same stress conditions. The analysis of the stress- induced transcripts and proximity of the transposon to the gene suggests that these TEs may provide local enhancer activities that stimulate stress-responsive gene expression. Our data on allelic variation for insertions of several of these TEs show strong correlation between the presence of TE insertions and stress-responsive up-regulation of gene expression. Our findings suggest that TEs provide an important source of allelic regulatory variation in gene response to abiotic stress in maize.

scholarly journals Genome Identification and CtCP1 Gene expression of Cysteine Protease in Carthamus Tinctorius under Abiotic Stress

2021 ◽  
Author(s):  
Yingqi Hong ◽  
Lv Yanxi ◽  
Zhang Jianyi ◽  
Nguyen Quoc Viet Hoang ◽  
Li Youbao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Abiotic Stress ◽  
Low Temperature ◽  
Gene Structure ◽  
Cysteine Protease ◽  
Carthamus Tinctorius ◽  
Whole Genome ◽  
Qrt Pcr ◽  
Transgenic Lines

Abstract BackgroundCysteine protease (CP) plays an important role in plant senescence. In this study, the whole genome evolution information of CPs was analyzed by using safflower, and the function of CtCP1 under l abiotic stress was analyzed.Results25 CPs members were identified in the safflower genome and divided into 9 subfamilies. Gene structure analysis showed that the possible evolutionary conservatism and functional similarity of the same family members. qRT-PCR at different florescence showed that the expression of CPs gene was the highest in the decline period, and CtCP1 gene changed significantly under abiotic stress. We cloned the qRT-PCR of CtCP1, at different florescence and stress, which showed that the expression of CtCP1 was the highest in the decline stage and low temperature. In order to study the function of CtCP1 gene, we obtained the overexpression CtCP1 line (OE) and the inhibition CtCP1 expression line (Anti) in Arabidopsis thaliana. The results of transgenic lines under low temperature stress showed that inhibition of CtCP1 expression enhanced the resistance of Carthamus tinctorius to low temperature, and overexpression of CtCP1 weakened the resistance of Carthamus tinctorius to low temperature.ConclusionWe have identified the cysteine protease genome of safflower and CtCP1 gene expression under abiotic stress

scholarly journals Label-free quantitative proteomic analysis of alfalfa in response to microRNA156 under high temperature

2020 ◽  
Author(s):  
Muhammad Arshad ◽  
Alpa Puri ◽  
Aaron J Simkovich ◽  
Justin Renaud ◽  
Margaret Y Gruber ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Heat Stress ◽  
Abiotic Stress ◽  
Stress Response ◽  
Protein Level ◽  
Forage Yield ◽  
Label Free ◽  
Multiple Traits ◽  
Positive Role ◽  
Empty Vector

Abstract Background: Abiotic stress, including heat, is one of the major factors that affect alfalfa growth and forage yield. The small RNA, microRNA156 (miR156), regulates multiple traits in alfalfa during abiotic stress. The aim of this study was to explore the role of miR156 in regulating heat response in alfalfa at the protein level. Results: In this study, we compared an empty vector control and miR156 overexpressing (miR156OE) alfalfa plants after exposing them to heat stress (40 °C) for 24 hours. We measured physiological parameters of control and miR156OE plants under heat stress, and collected leaf samples for protein analysis. A higher proline and antioxidant contents were detected in miR156OE plants than in controls under heat stress. Protein samples were analyzed by label-free quantification proteomics. Across all samples, a total of 1878 protein groups were detected. Under heat stress, 45 protein groups in the empty vector plants were significantly altered (P < 0.05; |log2FC| > 2). Conversely, 105 protein groups were significantly altered when miR156OE alfalfa was subjected to heat stress, of which 91 were unique to miR156OE plants. The identified protein groups unique to miR156OE plants were related to diverse functions including metabolism, photosynthesis, stress-response and plant defenses. Furthermore, we identified transcription factors in miR156OE plants, which belonged to squamosa promoter binding-like protein, MYB, ethylene responsive factors, AP2 domain, ABA response element binding factor and bZIP families of transcription factors. Conclusions: These results suggest a positive role for miR156 in heat stress response in alfalfa. They reveal a miR156-regulated network of mechanisms at the protein level to modulate heat responses in alfalfa.

scholarly journals Label-free quantitative proteomic analysis of alfalfa in response to microRNA156 under high temperature

BMC Genomics ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Muhammad Arshad ◽  
Alpa Puri ◽  
Aaron J. Simkovich ◽  
Justin Renaud ◽  
Margaret Y. Gruber ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Heat Stress ◽  
Abiotic Stress ◽  
Stress Response ◽  
Protein Level ◽  
Forage Yield ◽  
Label Free ◽  
Multiple Traits ◽  
Positive Role ◽  
Empty Vector

Abstract Background Abiotic stress, including heat, is one of the major factors that affect alfalfa growth and forage yield. The small RNA, microRNA156 (miR156), regulates multiple traits in alfalfa during abiotic stress. The aim of this study was to explore the role of miR156 in regulating heat response in alfalfa at the protein level. Results In this study, we compared an empty vector control and miR156 overexpressing (miR156OE) alfalfa plants after exposing them to heat stress (40 °C) for 24 h. We measured physiological parameters of control and miR156OE plants under heat stress, and collected leaf samples for protein analysis. A higher proline and antioxidant contents were detected in miR156OE plants than in controls under heat stress. Protein samples were analyzed by label-free quantification proteomics. Across all samples, a total of 1878 protein groups were detected. Under heat stress, 45 protein groups in the empty vector plants were significantly altered (P < 0.05; |log2FC| > 2). Conversely, 105 protein groups were significantly altered when miR156OE alfalfa was subjected to heat stress, of which 91 were unique to miR156OE plants. The identified protein groups unique to miR156OE plants were related to diverse functions including metabolism, photosynthesis, stress-response and plant defenses. Furthermore, we identified transcription factors in miR156OE plants, which belonged to squamosa promoter binding-like protein, MYB, ethylene responsive factors, AP2 domain, ABA response element binding factor and bZIP families of transcription factors. Conclusions These results suggest a positive role for miR156 in heat stress response in alfalfa. They reveal a miR156-regulated network of mechanisms at the protein level to modulate heat responses in alfalfa.

scholarly journals Label-free quantitative proteomic analysis of alfalfa in response to microRNA156 under high temperature

2020 ◽  
Author(s):  
Muhammad Arshad ◽  
Alpa Puri ◽  
Aaron J Simkovich ◽  
Justin Renaud ◽  
Margaret Y Gruber ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Heat Stress ◽  
Abiotic Stress ◽  
Stress Response ◽  
Protein Level ◽  
Forage Yield ◽  
Label Free ◽  
Multiple Traits ◽  
Positive Role ◽  
Empty Vector

Abstract Background: Abiotic stress, including heat, is one of the major factors that affect alfalfa growth and forage yield. The small RNA, microRNA156 (miR156), regulates multiple traits in alfalfa during abiotic stress. The aim of this study was to explore the role of miR156 in regulating heat response in alfalfa at the protein level. Results: In this study, we compared an empty vector control and miR156 overexpressing (miR156OE) alfalfa plants after exposing them to heat stress (40 ° C) for 24 hours. We measured physiological parameters of control and miR156OE plants under heat stress, and collected leaf samples for protein analysis. A higher proline and antioxidant contents were detected in miR156OE plants than in controls under heat stress. Protein samples were analyzed by label-free quantification proteomics. Across all samples, a total of 1878 protein groups were detected. Under heat stress, 45 protein groups in the empty vector plants were significantly altered (P < 0.05; |log 2 FC| > 2). Conversely, 105 protein groups were significantly altered when miR156OE alfalfa was subjected to heat stress, of which 91 were unique to miR156OE plants. The identified protein groups unique to miR156OE plants were related to diverse functions including metabolism, photosynthesis, stress-response and plant defenses. Furthermore, we identified transcription factors in miR156OE plants, which belonged to squamosa promoter binding-like protein, MYB, ethylene responsive factors, AP2 domain, ABA response element binding factor and bZIP families of transcription factors. Conclusions: These results suggest a positive role for miR156 in heat stress response in alfalfa. They reveal a miR156-regulated network of mechanisms at the protein level to modulate heat responses in alfalfa.

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