scholarly journals Effect of Cadmium-Tolerant Rhizobacteria on Growth Attributes and Chlorophyll Contents of Bitter Gourd under Cadmium Toxicity

Plants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1386 ◽  
Author(s):  
Muhammad Zafar-ul-Hye ◽  
Muhammad Naeem ◽  
Subhan Danish ◽  
Muhammad Jamil Khan ◽  
Shah Fahad ◽  
...  
Keyword(s):  
Cadmium Toxicity ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Antagonistic Effect ◽  
Bitter Gourd ◽  
Chlorophyll Contents ◽  
Plant Growth Promoting ◽  
Npk Fertilizer ◽  
Growth Attributes ◽  
Growth Promoting

Cadmium (Cd) is one of the heavy metals that negatively affects the growth of plants. High solubilization in water leads Cd to enter into plants quite easily, thus decreasing seed germination, photosynthesis, and transpiration. It also shows an antagonistic effect with many of the plants’ nutrients like Mn, Ca, K, Mg and Fe. Nowadays, inoculation of plants with ACC deaminase (ACCD) rhizobacteria to mitigate Cd’s adverse effects has drawn the attention of environmental microbiologists. The rhizobacteria secrete organic compounds that can immobilize Cd in soil. Therefore, this study was accomplished to investigate the effect of ACCD plant growth promoting rhizobacteria (PGPR) on the bitter gourd under Cd stress. There were six treatments consisting of two ACCD PGPR (Stenotrophomonas maltophilia and Agrobacterium fabrum) strains and inorganic fertilizers at two levels of Cd, i.e., 2 (Cd2) and 5 mg kg−1 soil (Cd5). The results showed A. fabrum with the recommended NPK fertilizer (RNPKF) significantly increased the vine length (48 and 55%), fresh weight (24 and 22%), and contents of chlorophyll a (79 and 50%), chlorophyll b (30 and 33%) and total chlorophyll (61 and 36%), over control at the two Cd levels i.e., Cd2 and Cd5, respectively. In conclusion, the recommended NPK fertilizer + A. fabrum combination is a very effective treatment with which to immobilize Cd in soil for the improvement of bitter gourd growth.

scholarly journals VEGETATIVE GROWTH OF RAINBOW CHILI (Capsicum annuum L.) IN THE TREATMENT OF PGPR (Plant Growth Promoting Rhizobacteria), PGPR-NPK FERTILIZER, AND PGPR-COMPOST COMBINATION

Agric ◽  
2020 ◽  
Vol 32 (2) ◽  
pp. 95-104
Author(s):  
Jennefer Constantia ◽  
Rejeki Siti Ferniah
Keyword(s):  
Vegetative Growth ◽  
Organic Fertilizer ◽  
Plant Growth Promoting Rhizobacteria ◽  
Optimal Growth ◽  
Randomized Design ◽  
Plant Growth Promoting ◽  
Npk Fertilizer ◽  
Growth Promoting ◽  
Biological Fertilizer

Bolivian Rainbow Chili is one of the ornamental chili varieties which is unique in the form of its colorful fruit color. The vegetative growth of ornamental chilies requires additional nutrients and the role of microorganisms to obtain optimal growth. This study aims to determine the optimal growth of rainbow chilies using the application of PGPR biological fertilizer, a combination of PGPR biological fertilizer and NPK synthetic fertilizer, as well as a combination of PGPR biological fertilizer and compost organic fertilizer. This research was conducted with a completely randomized design (CRD). The data obtained were then analyzed by ANOVA and continued with the LSD test and Duncan’s test at the 5% test stage. The results showed that the combination treatment of PGPR and compost or PGPR and NPK provided optimal vegetative growth compared to single PGPR treatment.

scholarly journals Characterization and optimization of 1-Aminocyclopropane-1-Carboxylate Deaminase (ACCD) activity in different rhizospheric PGPR along with Microbacterium sp. strain ECI-12A

2013 ◽  
Vol 1 (1) ◽  
pp. 11-15 ◽  
Author(s):  
Umesh P. Shrivastava ◽  
Ashok Kumar
Keyword(s):  
Plant Growth ◽  
Pcr Amplification ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Optimum Activity ◽  
Rice Rhizosphere ◽  
Acds Gene ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Culture Growth

A total of nine strains of plant growth promoting rhizobacteria were analyzed for ACC deaminase activity, where highest ACC deaminase activity was found in Klebsiella sp strain ECI-10A (539.1 nmol α-keto butyrate/ mg protein/ h) and lowest in Microbacterium sp strain ECI-12A (122.0 nmol α-keto butyrate/ mg protein/ h). Although Microbacterium sp strain ECI-12A showed lowest level of ACC deaminase activity, but, the species of Microbacterium isolated from rhizosphere is the first report. Microbacterium sp strain ECI-12A was also analyzed under varying conditions of time, amount of 1-Aminocyclopropane-1- carboxylate (ACC), and temperature for optimization of the ACC deaminase activity. The optimum activity was recorded with the supplementation of 5mM ACC at 30°C temperature after 24h of culture growth. All the nine strains showed acdS gene in the PCR amplification of that gene. No any rhizospheric Microbacterium species showing ACC deaminase activity have been reported earlier, therefore, we report here ACC deaminase activity in Microbacterium sp ECI-12A isolated from rice rhizosphere is a novel finding. DOI: http://dx.doi.org/10.3126/ijasbt.v1i1.7921 Int J Appl Sci Biotechnol, 2013, Vol. 1(1): 11-15

Isolation and Characterization of ACC Deaminase Gene from Two Plant Growth-Promoting Rhizobacteria

2008 ◽  
Vol 57 (4) ◽  
pp. 312-317 ◽  
Author(s):  
Venkadasamy Govindasamy ◽  
Murugesan Senthilkumar ◽  
Kishore Gaikwad ◽  
Kannepalli Annapurna
Keyword(s):  
Plant Growth ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals 1-Aminocyclopropane-1-Carboxylate: A Novel and Strong Chemoattractant for the Plant Beneficial Rhizobacterium Pseudomonas putida UW4

2019 ◽  
Vol 32 (6) ◽  
pp. 750-759 ◽  
Author(s):  
Tao Li ◽  
Jun Zhang ◽  
Chaohui Shen ◽  
Huiru Li ◽  
Liyou Qiu
Keyword(s):  
Pseudomonas Putida ◽  
Plant Root ◽  
Acc Oxidase ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Growth Promoting ◽  
Complementary Strain ◽  
Growth Promoting ◽  
Almost All ◽  
Gene Deletion Mutant

Plant growth–promoting rhizobacteria (PGPR) and fungi-bacterial biofilms are both important biofertilizer inoculants for sustainable agriculture. However, the strongest chemoattractant for bacteria to colonize the rhizosphere and mycelia is not clear. Coincidentally, almost all the PGPRs possess 1-aminocyclopropane-1-carboxylate (ACC) deaminase (AcdS) and can utilize ACC as the sole nitrogen source. Here, we found that ACC was a novel, metabolic dependent and methyl-accepting chemoreceptor–involved chemoattractant for Pseudomonas putida UW4. The chemotactic response of UW4 to ACC is significantly greater than that to the amino acids and organic acids identified in the plant root and fungal hyphal exudates. The colonization counts of the UW4 acdS or cheR deletion mutants in the wheat rhizosphere and on Agaricus bisporus mycelia were reduced one magnitude compared with those of UW4. The colonization counts of UW4 on A. bisporus antisense ACC oxidase mycelia with a high ACC production significantly increased compared with A. bisporus, followed by the UW4 cheR complementary strain and the ethylene chemoreceptor gene–deletion mutant. The colonization counts of the UW4 strains on A. bisporus acdS+ mycelia with a low ACC production decreased significantly compared with A. bisporus wild type. These results suggested that ACC and not ethylene should be the strongest chemoattractant for the PGPR that contain AcdS.

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