scholarly journals Functional Analysis of StPHT1;7, a Solanum tuberosum L. Phosphate Transporter Gene, in Growth and Drought Tolerance

Plants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1384
Author(s):  
Minxuan Cao ◽  
Hengzhi Liu ◽  
Chao Zhang ◽  
Dongdong Wang ◽  
Xiaofang Liu ◽  
...  
Keyword(s):  
Root Length ◽  
Solanum Tuberosum L ◽  
Potato Cultivar ◽  
Phosphate Transporter ◽  
Wild Type ◽  
Relative Expression ◽  
Potato Genome ◽  
Potato Plants ◽  
Overexpression Line ◽  
Bioinformatic Approaches

PHT1 (phosphate transporter 1) family genes play important roles in regulating plant growth and responding to stress. However, there has been little research on the role of the PHT1 family in potatoes. In this study, using molecular and bioinformatic approaches, 8 PHT1 family genes were identified from the potato genome. StPHT1;7 was highly expressed in the whole potato plants. The overexpression and silence vectors of StPHT1;7 were constructed and transformed into the potato cultivar Desiree. Consequently, StPHT1;7 overexpression (with a relative expression 2–7-fold that in the control) and silence lines (with a relative expression of 0.3%–1% that in the control) were obtained. Their growth vigor was ranked in the order overexpression line > wild type > silence line. In the absence of phosphorus, the root length of the overexpression line was approximately 2.6 times that of the wild type, while the root length of the silence line was approximately 0.6 times that of the wild type. Furthermore, their tolerance to drought stress was ranked as wild type > overexpression line > silence line. These results suggest that StPHT1;7 affects growth and stress tolerance in potato plants.

Efficiency of Transient Expression in Protoplasts of Various Potato Varieties

2020 ◽  
Vol 36 (3) ◽  
pp. 16-24
Author(s):  
S.R. Strelnikova ◽  
N.E. Zlobin ◽  
P.N. Kharchenko ◽  
R.A. Komakhin
Keyword(s):  
Solanum Tuberosum ◽  
Genome Editing ◽  
Transient Expression ◽  
Solanum Tuberosum L ◽  
Transient Gene Expression ◽  
Potato Cultivar ◽  
Potato Genome ◽  
Technological Stage ◽  
Genetic Constructs

Production of mesophilic protoplasts in potato (Solanum tuberosum L.) for transient gene expression is a mandatory technological stage in testing efficiency of new genetic constructs and CRISPR/Cas genome editing. In this study, isolation of protoplasts was carried out from leaves of potato plants of nine Russian cultivars grown in vitro for 6-7 weeks in vessels with foil caps, which prevented gas exchange with the environment. We first ever demonstrated the possibility of obtaining 2.4∙106 to 4.6∙106 viable protoplasts from one gram of aseptic plant leaves depending on the used potato cultivar. We established that the level of transfection of protoplasts with the genetic construct pHBT-sGFP-NosT amounts from 10 to 49% depending on the cultivar, which is sufficient for subsequent successful analysis of the efficiency of genetic constructs and potato genome editing. potato, Solanum tuberosum, protoplasts, plasmid DNA, transfection, transient expression, gfp The work was financially supported by the Comprehensive Research Program «Development of Potato Breeding and Seed Production».

scholarly journals Salicylic Acid Is Important for Basal Defense of Solanum tuberosum Against Phytophthora infestans

2007 ◽  
Vol 20 (11) ◽  
pp. 1346-1352 ◽  
Author(s):  
Vincentius A. Halim ◽  
Lennart Eschen-Lippold ◽  
Simone Altmann ◽  
Mandy Birschwilks ◽  
Dierk Scheel ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Solanum Tuberosum ◽  
Phytophthora Infestans ◽  
Isonicotinic Acid ◽  
Solanum Tuberosum L ◽  
Wild Type ◽  
Defense Gene Expression ◽  
Potato Plants ◽  
Basal Defense ◽  
Callose Formation

The importance of the signaling compound salicylic acid for basal defense of potato (Solanum tuberosum L. cv. Désirée) against Phytophthora infestans, the causal agent of late blight disease, was assessed using transgenic NahG potato plants which are unable to accumulate salicylic acid. Although the size of lesions caused by P. infestans was not significantly different in wild-type and transgenic NahG plants, real-time polymerase chain reaction analyses revealed a drastic enhancement of pathogen growth in potato plants depleted of salicylic acid. Increased susceptibility of NahG plants correlated with compromised callose formation and reduced early defense gene expression. NahG plants pretreated with the salicylic acid analog 2,6-dichloro-isonicotinic acid allowed pathogen growth to a similar extent as did wild-type plants, indicating that salicylic acid is an important compound required for basal defense of potato against P. infestans.

scholarly journals KEMAMPUAN DUA SPESIES Enterobacter sp. SEBAGAI BAKTERI PEMACU PERTUMBUHAN TANAMAN KENTANG (Solanum tuberosum L.) PADA KONDISI CEKAMAN KEKERINGAN

BUANA SAINS ◽  
2017 ◽  
Vol 17 (1) ◽  
pp. 85
Author(s):  
Khoirun Enisa Maharina ◽  
Nurul Aini ◽  
Luqman Qurata Aini
Keyword(s):  
Drought Stress ◽  
Root Length ◽  
Potato Plant ◽  
Endophytic Bacteria ◽  
Solanum Tuberosum L ◽  
Field Capacity ◽  
Stress Conditions ◽  
Plant Growth Promoting Bacteria ◽  
Vitro Assay ◽  
Potato Plants

Enterobacter xiangfangensis and Enterobacter cloaceae are known to have the ability as a phosfat solubilizing and producing hormone IAA. Their also have ability to survive under drought stress in vitro assay conditions. Both of these bacteria has good potential as a potato plant growth promoting bacteria, especially in drought stress conditions. This is an alternative to improve tolerance and growth of the potato crop in drought stress conditions. The results showed that the level of drought stress 75% of field capacity can boost the potential of endophytic bacteria E. xiangfangensis in increasing leaf area, plant dry weight, root length and physiological activity i.e total phosphorus content in potato plants. Endophytic bacteria E. xiangfangensis is better to increase potato plant height than endophytic bacteria E. cloaceae. These results are supported by the results of potential test of E. xiangfangensis which have the advantage of producing hormones IAA compared with E. cloaceae. Furthermore, in drought stress conditions 50% of field capacity and 75% of field capacity have different effects on the observations of root length growth parameters potato plants.

WEEDS IN POTATO CULTURE AND THEIR OUTCOME IN SPREADING OF Alternaria spp.

2018 ◽  
Vol 17 (6) ◽  
pp. 159-166
Author(s):  
Halina Kurzawińska ◽  
Stanisław Mazur ◽  
Małgorzata Nadziakiewicz ◽  
Jacek Nawrocki
Keyword(s):  
Chenopodium Album ◽  
Potato Cultivar ◽  
Cirsium Arvense ◽  
Potato Plants ◽  
Potato Culture ◽  
Rapd Pcr ◽  
Microscopic Features ◽  
Weed Infestation ◽  
Pcr Method ◽  
Potato Crops

The aim of this study was to determine whether the weeds accompanying potato crops can be a source of Alternaria spp. causing Alternaria leaf blight and to determine the genetic similarities of Alternaria alternata isolates infecting selected weeds: Chenopodium album, Cirsium arvense and tested potato cultivar. Three-year field experiment was conducted on the potato cultivar ‘Vineta N’. The isolates were classified into different species on the basis of macro- and microscopic features. In each year of the study, A. alternata dominated among the isolated fungi colonizing the leaves of potato plants and the selected weeds. The genetic similarities of A. alternata isolates was determined by the RAPD-PCR method. Tested genetic forms of A. alternata were closely related; only small differences in the pattern of the separated amplification products was evidenced. The dominance of A. alternata on the weeds accompanying potato crops suggests that if weed infestation is extensive, the pathogen is very likely to spread and its population to increase.

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro)

2019 ◽  
Vol 9 (2) ◽  
pp. 91
Author(s):  
Ghea Dotulong ◽  
Stella Umboh ◽  
Johanis Pelealu
Keyword(s):  
Solanum Tuberosum ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Leaf Extract ◽  
Solanum Tuberosum L ◽  
In Vitro Toxicity ◽  
Potato Plants ◽  
Colony Diameter ◽  
In Vitro Toxicity Test

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro) Ghea Dotulong1*), Stella Umboh1), Johanis Pelealu1), 1) Program Studi Biologi, FMIPA Universitas Sam Ratulangi, Manado 95115*Email korespondensi: [email protected] Diterima 9 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 Abstrak Tanaman kentang (Solanum tuberosum L.) adalah salah satu tanaman hortikultura yang sering mengalami penurunan dari segi produksi dan produktivitasnya, akibat adanya serangan penyakit layu yang salah satunya disebabkan oleh Fusarium oxysporum. Tujuan penelitian ini adalah mengidentifikasi toksisitas beberapa fungisida nabati dalam mengendalikan penyakit Layu Fusarium (F. oxysporum) pada tanaman kentang (Solanum tuberosum L.) secara In Vitro. Metode Penelitian yang digunakan yaitu metode umpan beracun. Data dianalisis dengan Rancangan Acak Lengkap (RAL) dengan Analisis Varian (ANAVA) yang dilanjutkan dengan menggunakan metode BNT (Beda Nyata Terkecil). Hasil Penelitian, diperoleh nilai toksisitas fungisida nabati tertinggi yaitu pada ekstrak daun sirsak dengan nilai HR (69,44%), kategori berpengaruh, ditandai dengan diameter koloni 2,75 cm (100ppm) dan yang terendah toksisitasnya yaitu pada ekstrak daun jeruk purut dengan nilai HR (49,81%), kategori cukup berpengaruh ditandai dengan diameter koloni 3,75 cm (25ppm). Semakin tinggi konsentrasi yang diujikan maka semakin tinggi toksisitas dari fungisida nabati yang diberikan.Kata Kunci: fungisida nabati, Fusarium oxysporum, tanaman kentang, In Vitro Abstract Potato plants (Solanum tuberosum L.) is one of the horticulture plants which often decreases in terms of production and productivity, due to the attack of wilt, one of which is caused by Fusarium oxysporum. The purpose of this study was to determine the toxicity of several biofungicides in controlling Fusarium wilt (F. oxysporum) in potato plants (Solanum tuberosum L.) in Vitro. The research method used was the In Vitro method with the poison bait method. Data were analyzed by Completely Randomized Design with Variant Analysis (ANAVA), followed by the BNT method. The results showed that the highest biofungicide toxicity value was soursop leaf extract with HR values (69.44%), influential categories, characterized by colony diameter 2.75 cm (100ppm) and the lowest toxicity, namely in kaffir lime leaf extract with a value of HR (49.81%), quite influential category was characterized by colony diameter of 3.75 cm (25ppm). The higher the concentration tested, the higher the toxicity of the biofungicide given.Keywords: biofungicides, Fusarium oxysporum, Potato Plants, In Vitro.

scholarly journals Expression Level of Mature miR172 in Wild Type and StSUT4-Silenced Plants of Solanum tuberosum Is Sucrose-Dependent

2021 ◽  
Vol 22 (3) ◽  
pp. 1455
Author(s):  
Varsha Garg ◽  
Aleksandra Hackel ◽  
Christina Kühn
Keyword(s):  
Solanum Tuberosum ◽  
Wild Type ◽  
Potato Plants ◽  
Mature Leaves ◽  
In Vitro Plantlets ◽  
Long Time ◽  
Short Time ◽  
Cut Leaves

In potato plants, the phloem-mobile miR172 is involved in the sugar-dependent transmission of flower and tuber inducing signal transduction pathways and a clear link between solute transport and the induction of flowering and tuberization was demonstrated. The sucrose transporter StSUT4 seems to play an important role in the photoperiod-dependent triggering of both developmental processes, flowering and tuberization, and the phenotype of StSUT4-inhibited potato plants is reminiscent to miR172 overexpressing plants. The first aim of this study was the determination of the level of miR172 in sink and source leaves of StSUT4-silenced as well as StSUT4-overexpressing plants in comparison to Solanum tuberosum ssp. Andigena wild type plants. The second aim was to investigate the effect of sugars on the level of miRNA172 in whole cut leaves, as well as in whole in vitro plantlets that were supplemented with exogenous sugars. Experiments clearly show a sucrose-dependent induction of the level of mature miR172 in short time as well as long time experiments. A sucrose-dependent accumulation of miR172 was also measured in mature leaves of StSUT4-silenced plants where sucrose export is delayed and sucrose accumulates at the end of the light period.

scholarly journals Silicon reduces aluminum content in tissues and ameliorates its toxic effects on potato plant growth

2016 ◽  
Vol 46 (3) ◽  
pp. 506-512 ◽  
Author(s):  
Athos Odin Severo Dorneles ◽  
Aline Soares Pereira ◽  
Liana Verônica Rossato ◽  
Gessieli Possebom ◽  
Victória Martini Sasso ◽  
...  
Keyword(s):  
Chemical Element ◽  
Shoot Growth ◽  
Aluminum Content ◽  
Solanum Tuberosum L ◽  
Toxic Effects ◽  
Al Content ◽  
Potato Plants ◽  
High Affinity ◽  
Root And Shoot Growth ◽  
Tolerant Genotype

ABSTRACT: Aluminum (Al) is highly toxic to plants, causing stress and inhibiting growth and silicon (Si) is considered beneficial for plants. This chemical element has a high affinity with Al. The aim of this study was to investigate the potential of Si to mitigate the toxic effects of Al on potato ( Solanum tuberosum L.) plants and assess whether this behavior is different among genotypes with differing degrees of sensitivity to Al. Potato plants of the genotypes SMIJ319-7 (Al-sensitive) and SMIF212-3 (Al-tolerant) were grown for fourteen days in nutrient solution (without P and pH 4.5±0.1) under exposure to combinations of Al (0 and 1.85mM) and Si (0, 0.5 and 1.0mM). After this period, shoot and roots of the two genotypes were collected to determine Al content in tissues and assess morphological parameters of root and shoot growth. Roots of both genotypes accumulated more Al than shoots and the Al-tolerant genotype accumulated more Al than the sensitive one, both in roots and in shoot. Furthermore, the presence of 0.5 and 1.0mM Si together with Al reduced the Al content in shoot in both genotypes and in roots of the Al-tolerant genotype, respectively. Si ameliorated the toxic effects of Al with regard to number of root branches and leaf number in both potato genotypes. Si has the potential to mitigate the toxic effects of Al in potato plants regardless of Al sensitivity.

Uptake of Plutonium-238 into Solanum tuberosum L. (potato plants) in presence of complexing agent EDTA

2017 ◽  
Vol 178-179 ◽  
pp. 186-192
Author(s):  
Frank Tawussi ◽  
Dharmendra K. Gupta ◽  
Elena L. Mühr-Ebert ◽  
Stephanie Schneider ◽  
Stefan Bister ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Complexing Agent ◽  
Potato Plants

scholarly journals Novel Nanobiocomposites Based on Natural Polysaccharides as Universal Trophic Low-Dose Micronutrients

2021 ◽  
Vol 22 (21) ◽  
pp. 12006
Author(s):  
Spartak S. Khutsishvili ◽  
Alla I. Perfileva ◽  
Olga A. Nozhkina ◽  
Tatjana V. Ganenko ◽  
Konstantin V. Krutovsky
Keyword(s):  
Low Dose ◽  
Solanum Tuberosum L ◽  
Soil Microflora ◽  
Natural Soil ◽  
Plant Tissues ◽  
Paramagnetic Resonance ◽  
Phytopathogenic Bacterium ◽  
Toxicological Effects ◽  
Potato Plants ◽  
Electron Paramagnetic

New promising manganese-containing nanobiocomposites (NCs) based on natural polysaccharides, arabinogalactan (AG), arabinogalactan sulfate (AGS), and κ-carrageenan (κ-CG) were studied to develop novel multi-purpose trophic low-dose organomineral fertilizers. The general toxicological effects of manganese (Mn) on the vegetation of potatoes (Solanum tuberosum L.) was evaluated in this study. The essential physicochemical properties of this trace element in plant tissues, such as its elemental analysis and its spectroscopic parameters in electron paramagnetic resonance (EPR), were determined. Potato plants grown in an NC-containing medium demonstrated better biometric parameters than in the control medium, and no Mn accumulated in plant tissues. In addition, the synthesized NCs demonstrated a pronounced antibacterial effect against the phytopathogenic bacterium Clavibacter sepedonicus (Cms) and were proved to be safe for natural soil microflora.

scholarly journals Comprehensive Genome-Wide Analysis of The Catalase Enzyme Toolbox In Potato (Solanum Tuberosum L.)

2021 ◽  
Author(s):  
Rania Jbir Koubaa ◽  
Mariem Ayadi ◽  
Mohamed Najib Saidi ◽  
Safa Charfeddine ◽  
Radhia Gargouri Bouzid ◽  
...  
Keyword(s):  
Stress Responses ◽  
Expression Profiling ◽  
Phylogenetic Trees ◽  
Solanum Tuberosum L ◽  
Potato Cultivar ◽  
Promoter Sequence ◽  
3D Protein Structure ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
A Genome

Abstract As antioxidant enzymes, catalase (CAT) protects organisms from oxidative stress via the production of reactive oxygen species (ROS). These enzymes play important roles in diverse biological processes. However, little is known about the CAT genes in potato plants despite its important economical rank of this crop in the world. Yet, abiotic and biotic stresses severely hinder growth and development of the plants which affects the production and quality of the crop. To define the possible roles of CAT genes under various stresses, a genome-wide analysis of CAT gene family has been performed in potato plant.In this study, the StCAT gene’s structure, secondary and 3D protein structure, physicochemical properties, synteny analysis, phylogenetic tree and also expression profiling under various developmental and environmental cues were predicted using bioinformatics tools. The expression analysis by RT-PCR was performed using commercial potato cultivar. Three genes encoding StCAT that code for three proteins each of size 492 aa, interrupted by seven introns have been identified in potatoes. StCAT proteins were found to be localized in the peroxisome which is judged as the main H2O2 cell production site during different processes. Many regulating cis-elements related to stress responses and plant hormones signaling were found in the promoter sequence of each gene. The analysis of motifs and phylogenetic trees showed that StCAT are closer to their homologous in S. lycopersicum and share a 41% – 95% identity with other plants’ CATs. Expression profiling revealed that StCAT1 is the constitutively expressive member; while StCAT2 and StCAT3 are the stress-responsive members.

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