scholarly journals Identification and Bioinformatic Analysis of the GmDOG1-Like Family in Soybean and Investigation of Their Expression in Response to Gibberellic Acid and Abscisic Acid

Plants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 937
Author(s):  
Yingzeng Yang ◽  
Chuan Zheng ◽  
Umashankar Chandrasekaran ◽  
Liang Yu ◽  
Chunyan Liu ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Seed Germination ◽  
Gibberellic Acid ◽  
Seed Dormancy ◽  
Expression Patterns ◽  
Evolutionary Relationship ◽  
Soybean Seed ◽  
Bioinformatic Analysis ◽  
Chromosomal Distribution ◽  
Seed Dormancy And Germination

Seed germination is one of the most important stages during plant life cycle, and DOG1 (Delay of germination1) plays a pivotal regulatory role in seed dormancy and germination. In this study, we have identified the DOG1-Like (DOG1L) family in soybean (Glycine max), a staple oil crop worldwide, and investigated their chromosomal distribution, structure and expression patterns. The results showed that the GmDOG1L family is composed of 40 members, which can be divided into six subgroups, according to their evolutionary relationship with other known DOG1-Like genes. These GmDOG1Ls are distributed on 18 of 20 chromosomes in the soybean genome and the number of exons for all the 40 GmDOG1Ls varied greatly. Members of the different subgroups possess a similar motif structure composition. qRT-PCR assay showed that the expression patterns of different GmDOG1Ls were significantly altered in various tissues, and some GmDOG1Ls expressed primarily in soybean seeds. Gibberellic acid (GA) remarkably inhibited the expression of most of GmDOG1Ls, whereas Abscisic acid (ABA) inhibited some of the GmDOG1Ls expression while promoting others. It is speculated that some GmDOG1Ls regulate seed dormancy and germination by directly or indirectly relating to ABA and GA pathways, with complex interaction networks. This study provides an important theoretical basis for further investigation about the regulatory roles of GmDOG1L family on soybean seed germination.

scholarly journals Regulation of Seed Dormancy and Germination Mechanisms in a Changing Environment

2021 ◽  
Vol 22 (3) ◽  
pp. 1357
Author(s):  
Ewelina A. Klupczyńska ◽  
Tomasz A. Pawłowski
Keyword(s):  
Climate Change ◽  
Seed Germination ◽  
Seed Dormancy ◽  
Environmental Conditions ◽  
Global Climate ◽  
Plant Evolution ◽  
Suitable Habitat ◽  
Climate Change Scenarios ◽  
Adaptation Mechanism ◽  
Seed Dormancy And Germination

Environmental conditions are the basis of plant reproduction and are the critical factors controlling seed dormancy and germination. Global climate change is currently affecting environmental conditions and changing the reproduction of plants from seeds. Disturbances in germination will cause disturbances in the diversity of plant communities. Models developed for climate change scenarios show that some species will face a significant decrease in suitable habitat area. Dormancy is an adaptive mechanism that affects the probability of survival of a species. The ability of seeds of many plant species to survive until dormancy recedes and meet the requirements for germination is an adaptive strategy that can act as a buffer against the negative effects of environmental heterogeneity. The influence of temperature and humidity on seed dormancy status underlines the need to understand how changing environmental conditions will affect seed germination patterns. Knowledge of these processes is important for understanding plant evolution and adaptation to changes in the habitat. The network of genes controlling seed dormancy under the influence of environmental conditions is not fully characterized. Integrating research techniques from different disciplines of biology could aid understanding of the mechanisms of the processes controlling seed germination. Transcriptomics, proteomics, epigenetics, and other fields provide researchers with new opportunities to understand the many processes of plant life. This paper focuses on presenting the adaptation mechanism of seed dormancy and germination to the various environments, with emphasis on their prospective roles in adaptation to the changing climate.

scholarly journals Rice GERMIN-LIKE PROTEIN 2-1 Functions in Seed Dormancy under the Control of Abscisic Acid and Gibberellic Acid Signaling Pathways

2020 ◽  
Vol 183 (3) ◽  
pp. 1157-1170 ◽  
Author(s):  
Haiting Wang ◽  
Yuman Zhang ◽  
Na Xiao ◽  
Ge Zhang ◽  
Fang Wang ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Gibberellic Acid ◽  
Signaling Pathways ◽  
Seed Dormancy ◽  
Gibberellic Acid Signaling

scholarly journals Methods of breaking seed dormancy for ornamental passion fruit species

2017 ◽  
Vol 23 (1) ◽  
pp. 72 ◽  
Author(s):  
Thalita Neves Marostega ◽  
Petterson Baptista Da Luz ◽  
Armando Reis Tavares ◽  
Leonarda Grillo Neves ◽  
Severino De Paiva Sobrinho
Keyword(s):  
Experimental Design ◽  
Seed Germination ◽  
Gibberellic Acid ◽  
Seed Dormancy ◽  
Potassium Nitrate ◽  
Plant Production ◽  
Commercial Plant ◽  
Fruit Species ◽  
Breaking Seed Dormancy ◽  
And Control

The Passiflora L. genus covers a diversity of wild species with ornamental potential, especially due to the intrinsic beauty of its exotic flowers, flowering more than once a year and the lush foliage. However, Passiflora seeds present dormancy complicating seed germination and the establishment of commercial plant production with species with high ornamental potential. This study was conducted to determine the best pre-germination treatments to overcome seed dormancy for Passiflora quadrangularis, P. nitida, P. foetida, P. eichleriana, P. alata, P. cincinnata, P. mucronata, P. micropetala, P. suberosa, P. morifolia and P. tenuifila. The experimental design was completely randomized, with five treatments and four replicates, with 25 seeds per plot. Pre-germination treatments were: seeds soaked in 1,000 mg L- 1 GA3 (gibberellic acid) for 6 hours, seeds soaked in 0.2 % KNO3 (potassium nitrate) for 24 hours, seeds soaked in 1 % KNO3 for 24 hours, partial seedcoat scarification with sandpaper number 120 and control (seeds untreated). Percentage of germination, germination velocity index and radicle length were evaluated for all species. The results showed that GA3 was effective to overcome seed dormancy in P. suberosa (86%), P. morifolia (68 %) and P. tenuifila (54%). KNO3 1% had significant effect on overcoming dormancy in seeds of P. eichleriana (66%) and scarification with sandpaper increased seed germination of P. micropetala (38%).

scholarly journals Seed dormancy and germination in Cardiocrinum giganteum var. yunnanense, a perennial herb in China with post-dispersal embryo growth

2020 ◽  
Vol 48 (2) ◽  
pp. 303-314
Author(s):  
Ye-Fang Li ◽  
Jie Song ◽  
Wen-Ling Guan ◽  
Feng-Rong Li
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Practical Knowledge ◽  
Perennial Herb ◽  
Late Winter ◽  
Dormancy Breaking ◽  
Morphophysiological Dormancy ◽  
Summer Temperatures ◽  
Autumn And Winter ◽  
Seed Dormancy And Germination

Seeds of Cardiocrinum giganteum var. yunnanense, which is native to China, has underdeveloped embryos when dispersed from parent plants that did not grow until the second autumn and winter after exposure to summer temperatures. Radicles and cotyledons emerged in late winter and spring. Thus, a 15–16 month period was required from dispersal to seed germination. Under laboratory conditions, this period could be shortened to 5–6 months in a 25°C/15°C (60 days) → 15°C/5°C (60 days) → 5°C (60 days) temperature sequence. Based on dormancy-breaking requirements, the seeds have deep simple morphophysiological dormancy (MPD). This is practical knowledge for propagation of the species from seeds.

The effect of sodium hypochlorite and gibberellic acid on seed dormancy and germination of wild oats (Avena fatua)

1979 ◽  
Vol 57 (16) ◽  
pp. 1729-1734 ◽  
Author(s):  
A. I. Hsiao
Keyword(s):  
Gibberellic Acid ◽  
Seed Dormancy ◽  
Sodium Hypochlorite ◽  
Slow Rate ◽  
Avena Fatua ◽  
Germination Inhibitors ◽  
Wild Oats ◽  
Dormancy Induction ◽  
Seed Dormancy And Germination

The seed coverings, including the pericarp and testa of the caryopsis and the hull, arc the main barriers to the exchange of gases and the penetration of exogenous gibberellic acid (GA3) for germination of wild oats (Avena fatua L.). Dormancy was induced in seeds by immersing them in water for 15 minor longer. Dormancy induction was greater in those seeds immersed for up to 1 h in 6% sodium hypochlorite (NaOCl) and then 1 h in water than in those seeds immersed only in water for 1 h. The addition of GA3, to seeds subjected to NaOCl treatment for 15 min or less did not break dormancy, indicating a slow rate of entry, or the exclusion, of GA3, by the seeds. In the presence of GA3, germination increased with increasing exposure to NaOCl. Maximum germination was obtained by immersing dry seeds in NaOCl for 2 h, in water for 1 h, and then incubating the seeds in GA3. Gibberellic acid was not required for complete germination of imbibed, dehulled seeds immersed in NaOCl for 1 h then in water for 1 h, but it was necessary to use 10−4 M GA3 for complete germination of intact seeds that were treated with NaOCl or 2 h then with water for 1 h. Imbibed, dormant seeds that were dehulled and pierced required 10−7 M GA3, to give complete germination in this study. Piercing of the seed coverings enhances GA3, penetration and thus increases the availability of GA3, for germination. NaOCl treatment to the seeds mimics the effects of piercing. NaOCl may also have caused loss of germination inhibitors or rendered these inhibitors susceptible to oxidation. However, prolonged NaOCl treatment resulted in either poor germination or seed disintegration.

scholarly journals Genome-wide identification, phylogeny and expression analysis of the SPL gene family in wheat

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Ting Zhu ◽  
Yue Liu ◽  
Liting Ma ◽  
Xiaoying Wang ◽  
Dazhong Zhang ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Gene Family ◽  
Gene Structure ◽  
Expression Patterns ◽  
Wheat Yield ◽  
Chromosomal Distribution ◽  
Positive Role ◽  
Qrt Pcr ◽  
Genome Wide ◽  
Duplication Events

Abstract Background Members of the plant-specific SPL gene family (squamosa promoter-binding protein -like) contain the SBP conserved domain and are involved in the regulation of plant growth and development, including the development of plant flowers and plant epidermal hair, the plant stress response, and the synthesis of secondary metabolites. This family has been identified in various plants. However, there is no systematic analysis of the SPL gene family at the genome-wide level of wheat. Results In this study, 56 putative TaSPL genes were identified using the comparative genomics method; we renamed them TaSPL001 - TaSPL056 on their chromosomal distribution. According to the un-rooted neighbor joining phylogenetic tree, gene structure and motif analyses, the 56 TaSPL genes were divided into 8 subgroups. A total of 81 TaSPL gene pairs were designated as arising from duplication events and 64 interacting protein branches were identified as involve in the protein interaction network. The expression patterns of 21 randomly selected TaSPL genes in different tissues (roots, stems, leaves and inflorescence) and under 4 treatments (abscisic acid, gibberellin, drought and salt) were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Conclusions The wheat genome contains 56 TaSPL genes and those in same subfamily share similar gene structure and motifs. TaSPL gene expansion occurred through segmental duplication events. Combining the results of transcriptional and qRT-PCR analyses, most of these TaSPL genes were found to regulate inflorescence and spike development. Additionally, we found that 13 TaSPLs were upregulated by abscisic acid, indicating that TaSPL genes play a positive role in the abscisic acid-mediated pathway of the seedling stage. This study provides comprehensive information on the SPL gene family of wheat and lays a solid foundation for elucidating the biological functions of TaSPLs and improvement of wheat yield.

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