scholarly journals Transcriptome Profiling Provides Molecular Insights into Auxin-Induced Adventitious Root Formation in Sugarcane (Saccharum spp. Interspecific Hybrids) Microshoots

Plants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 931 ◽  
Author(s):  
Aomei Li ◽  
Prakash Lakshmanan ◽  
Weizhong He ◽  
Hongwei Tan ◽  
Limin Liu ◽  
...  
Keyword(s):  
Adventitious Root ◽  
Root Formation ◽  
Expression Patterns ◽  
Transcriptome Profiling ◽  
Adventitious Root Formation ◽  
Pcr Analysis ◽  
Naphthalene Acetic Acid ◽  
Rna Seq ◽  
Cell Wall Modification ◽  
Plant Hormone Signaling

Adventitious root (AR) formation was enhanced following the treatment of sugarcane microshoots with indole-3-butyric acid (IBA) and 1-naphthalene acetic acid (NAA) combined, suggesting that auxin is a positive regulator of sugarcane microshoot AR formation. The transcriptome profile identified 1737 and 1268 differentially expressed genes (DEGs) in the basal tissues (5 mm) of sugarcane microshoots treated with IBA+NAA compared to nontreated control on the 3rd and 7th days post-auxin or water treatment (days post-treatment—dpt), respectively. To understand the molecular changes, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed. This analysis showed that DEGs associated with the pathways were associated with plant hormone signaling, flavonoid and phenylpropanoid biosyntheses, cell cycle, and cell wall modification, and transcription factors could be involved in sugarcane microshoot AR formation. Furthermore, qRT–PCR analysis was used to validate the expression patterns of nine genes associated with root formation and growth, and the results were consistent with the RNA-seq results. Finally, a hypothetical hormonal regulatory working model of sugarcane microshoot AR formation is proposed. Our results provide valuable insights into the molecular processes associated with auxin-induced AR formation in sugarcane.

scholarly journals Transcriptomic profiling and discovery of key genes involved in adventitious root formation from green cuttings of highbush blueberry (Vaccinium corymbosum L.)

2020 ◽  
Author(s):  
Haishan An ◽  
Jiaying Zhang ◽  
Fangjie Xu ◽  
Shuang Jiang ◽  
Xueying Zhang
Keyword(s):  
Candidate Genes ◽  
Transcriptome Analysis ◽  
Adventitious Root ◽  
Root Formation ◽  
Transcriptome Profiling ◽  
Vaccinium Corymbosum ◽  
Adventitious Root Formation ◽  
Root Characteristics ◽  
Indole Butyric Acid ◽  
Root Primordium

Abstract Background: Propagation of cuttings is frequently used in various plant species, including blueberry, which shows special root characteristics that may hinder adventitious root (AR) formation. AR formation is influenced by various factors, and auxin is considered to play a central role; however, little is known of the related regulatory mechanisms. In this study, a comparative transcriptome analysis of green cuttings treated with or without indole-butyric acid (IBA) was performed via RNA_seq to identify candidate genes associated with IBA-induced AR formation.Results: Rooting phenotypes, especially the rooting rate, were significantly promoted by exogenous auxin in the IBA application. Blueberry AR formation was an auxin-induced process, during which adventitious root primordium initiation (rpi) began at 14 days after cutting (DAC), root primordium (rp) was developed at 21 DAC, mature AR was observed at 28 DAC and finally outgrowth from the stem occurred at 35 DAC. Higher IAA levels and lower ABA and zeatin contents might facilitate AR formation and development. A time series transcriptome analysis identified 14970 differentially expressed genes (DEGs) during AR formation, of which there were 7467 upregulated and 7503 downregulated genes. Of these, approximately 35 candidate DEGs involved in the auxin-induced pathway and AR formation were further identified, including 10 auxin respective genes (ARFs and SAURs), 13 transcription factors (LOB domain-containing protein (LBDs)), 6 auxin transporters (AUX22, LAX3/5 and PIN-like 6 (PIL6s)) and 6 rooting-associated genes (root meristem growth factor 9 (RGF9), lateral root primordium 1 (LRP1s), and dormancy-associated protein homologue 3 (DRMH3)). All these identified DEGs were highly upregulated in certain stages during AR formation, indicating their potential roles in blueberry AR formation.Conclusions: The transcriptome profiling results indicated candidate genes or major regulatory factors that influence adventitious root formation in blueberry and provided a comprehensive understanding of the rooting mechanism underlying the auxin-induced AR formation from blueberry green cuttings.

scholarly journals Transcriptomic profiling and discovery of key genes involved in adventitious root formation from green cuttings of highbush blueberry (Vaccinium corymbosum L.)

2020 ◽  
Author(s):  
Haishan An ◽  
Jiaying Zhang ◽  
Fangjie Xu ◽  
Shuang Jiang ◽  
Xueying Zhang
Keyword(s):  
Candidate Genes ◽  
Transcriptome Analysis ◽  
Adventitious Root ◽  
Root Formation ◽  
Transcriptome Profiling ◽  
Vaccinium Corymbosum ◽  
Adventitious Root Formation ◽  
Root Characteristics ◽  
Indole Butyric Acid ◽  
Root Primordium

Abstract Background: Propagation of cuttings is frequently used in various plant species, including blueberry, which shows special root characteristics that may hinder adventitious root (AR) formation. AR formation is influenced by various factors, and auxin is considered to play a central role; however, little is known of the related regulatory mechanisms. In this study, a comparative transcriptome analysis of green cuttings treated with or without indole-butyric acid (IBA) was performed via RNA_seq to identify candidate genes associated with IBA-induced AR formation. Results: Rooting phenotypes, especially the rooting rate, were significantly promoted by exogenous auxin in the IBA application. Blueberry AR formation was an auxin-induced process, during which adventitious root primordium initiation (rpi) began at 14 days after cutting (DAC), root primordium (rp) was developed at 21 DAC, mature AR was observed at 28 DAC and finally outgrowth from the stem occurred at 35 DAC. Higher IAA levels and lower ABA and zeatin contents might facilitate AR formation and development. A time series transcriptome analysis identified 14970 differentially expressed genes (DEGs) during AR formation, of which there were 7467 upregulated and 7503 downregulated genes. Of these, approximately 35 candidate DEGs involved in the auxin-induced pathway and AR formation were further identified, including 10 auxin respective genes ( ARFs and SAURs ), 13 transcription factors ( LOB domain-containing protein ( LBD s)), 6 auxin transporters ( AUX22 , LAX3/5 and PIN-like 6 ( PIL6s )) and 6 rooting-associated genes ( root meristem growth factor 9 ( RGF9 ), lateral root primordium 1 ( LRP1s ), and dormancy-associated protein homologue 3 ( DRMH3 )). All these identified DEGs were highly upregulated in certain stages during AR formation, indicating their potential roles in blueberry AR formation. Conclusions: The transcriptome profiling results indicated candidate genes or major regulatory factors that influence adventitious root formation in blueberry and provided a comprehensive understanding of the rooting mechanism underlying the auxin-induced AR formation from blueberry green cuttings.

scholarly journals Transcriptomic profiling and discovery of key genes involved in adventitious root formation from green cuttings of highbush blueberry (Vaccinium corymbosum L.)

2019 ◽  
Author(s):  
Haishan An ◽  
Jiaying Zhang ◽  
Fangjie Xu ◽  
Shuang Jiang ◽  
Xueying Zhang
Keyword(s):  
Candidate Genes ◽  
Transcriptome Analysis ◽  
Adventitious Root ◽  
Root Formation ◽  
Transcriptome Profiling ◽  
Vaccinium Corymbosum ◽  
Adventitious Root Formation ◽  
Highbush Blueberry ◽  
Root Characteristics ◽  
Root Primordium

Abstract Background Propagation of cuttings was mostly used in various plant species including blueberry, the special root characteristics of blueberry usually resulted in a difficulty in adventitious root (AR) formation. The AR formation was influenced by various factors, of which auxin was considered to play a center role, however little is known of the related regulative mechanisms. In this study, transcriptome analysis using RNA_seq from the stem of green cuttings of southern highbush blueberry 'Biloxi' was performed to discover candidate genes associated with AR formation.Results Rooting phenotypes, especially rooting rate, was significantly promoted by exogenous auxin IBA application. The adventitious root primordium initiation (rpi) began to be formed at 14 day (d) after cutting, developed into root primordium (rp) at 21d, finally the rp further developed to mature AR at 28d. Higher IAA and lower ABA and zeatin might facilitate the AR formation and development. A time series transcriptome analysis indentified 14970 differentially expressed genes (DEGs) during AR formation, of which there were 7467 up-regulated and 7503 down-regulated genes, respectively. Of these, about 35 candidate DEGs involved in auxin-induced pathway and AR formation were further identified, including 10 auxin respective genes ARFs and SAURs , 13 transcription factors LOB domain-containing protein ( LBD s), 6 auxin transporter AUX22 , LAX3/5 and PIN-like 6s ( PIL6s ) and 6 rooting-associated genes root meristem growth factor 9 ( RGF9 ), lateral root primordium 1 ( LRP1s ), dormancy-associated protein homolog 3 ( DRMH3 ). All these identified DEGs were highly up-regulated in certain AR developed stage, indicating their potential roles in blueberry AR formation.Conclusions The transcriptome profiling indicated candidate genes or major regulative factors that influence adventitious root formation in blueberry, and provided a comprehensive understanding of rooting mechanism of the auxin-induced AR formation from blueberry green cuttings.

scholarly journals Adventitious root formation in branch cuttings of Taxus wallichiana Zucc.(Himalayan yew): A clonal approach to conserve the scarce resource

2017 ◽  
Vol 8 ◽  
Author(s):  
Mohd Aslam ◽  
Parvaiz A. Raina ◽  
Rouf Ur Rafiq ◽  
T.O. Siddiqi ◽  
Zafar A. Reshi
Keyword(s):  
Acetic Acid ◽  
Adventitious Root ◽  
Root Formation ◽  
Block Design ◽  
Natural Habitat ◽  
Adventitious Root Formation ◽  
Naphthalene Acetic Acid ◽  
Indole Butyric Acid ◽  
Himalayan Yew ◽  
Taxus Wallichiana

Himalayan yew (<em>Taxus wallichiana</em> Zucc.), is an economically valuable plant and critically endangered due to overexploitation for the isolation of Taxol,an exciting anticancer drug from its bark and leaves. Since the species is unisexual and due to its long seed dormancy period and rapid loss of viability coupled with low survival percentage, its natural regeneration from seeds is very poor. As the seed raised plants add little growth, propagation by stem cuttings was tried under natural conditions and a considerable success was achieved after making use of different auxins (Indole-3- Acetic Acid-IAA, Indole Butyric Acid-IBA and Naphthalene Acetic Acid-NAA) in different concentrations. A Randomized block design (RBD) was adopted for laying the experiment of the present study. Of the ten treatments studied, IBA at 500ppm performed best of all the treatments and registered higher callusing percentage, rooting percentage, number of roots and length of roots in the juvenile shoot cuttings of the species. The results achieved through the application ofplant growth regulators (PGRs)by way of adventitious root formation (ARF) could be useful for the management of this understory coniferous tree species, whether for conservation, habitat restoration or for the production of Taxol,a promising anti-cancer agent. The technique evolved will be the most handy, quickest, inexpensive and can be applied any where in its natural habitat for the restoration and restocking of this valuable plant, which is otherwise facing the peril of extinction throughout the range of its distribution including Indian Himalayas.

scholarly journals Analysis of Horticultural Performance of Ethylene-insensitive Petunias and Tomatoes

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 499D-499
Author(s):  
E.K. Gubrium ◽  
D.G. Clark ◽  
H.J. Klee ◽  
T.A. Nell ◽  
J.E. Barrett
Keyword(s):  
Fruit Ripening ◽  
Adventitious Root ◽  
Root Formation ◽  
Developmental Stages ◽  
Stem Elongation ◽  
Mutant Gene ◽  
Adventitious Root Formation ◽  
Model Systems ◽  
Pcr Analysis ◽  
Wild Type

We are studying the horticultural performance of two model plant systems that carry a mutant gene that confers ethylene-insensitivity: Never Ripe tomatoes and petunia plants transformed with the mutant etr1-1 gene isolated from Arabidopsis thaliana. Having two model systems to compare side-by-side allows us to determine with greater certainty ethylene's role at different developmental stages. Presence of the mutant etr1-1 gene in transgenic petunias was determined using three techniques: PCR analysis, the seedling triple response assay (inhibition of stem elongation, radial swelling of stem and roots, and an exaggerated apical hook when grown in the dark and in the presence of ethylene), and the flower wilting response to pollination, which is known to be induced by ethylene. Flowers from ethylene-insensitive petunias took almost four times as long to wilt after pollination as wild-type plants. It is well known that fruit ripening in Never Ripe tomato is inhibited, and a similar delayed fruit ripening phenotype is observed in petunia plants transformed with etr1-1. In an effort to maintain ethylene-insensitive petunia plants by vegetative propagation, we observed that the rate of adventitious root formation was much lower with transgenic plants than in wild-type plants. In subsequent experiments on adventitious root formation in Never Ripe tomato, we observed the same result. Therefore, while ethylene-insensitive tomato and petunia plants appear phenotypically normal for many characters, other factors are altered by the presence of this mutation. The fact that these changes are present in two model systems helps to define the role of ethylene perception in plant growth and reproduction.

Erratum to: Transcriptome profiling reveals an IAA-regulated response to adventitious root formation in lotus seedling

2018 ◽  
Vol 73 (9-10) ◽  
pp. 409-409
Author(s):  
Cheng Libao ◽  
Jiang Runzhi ◽  
Yang Jianjun ◽  
Xu Xiaoyong ◽  
Zeng Haitao ◽  
...  
Keyword(s):  
Adventitious Root ◽  
Root Formation ◽  
Transcriptome Profiling ◽  
Adventitious Root Formation

scholarly journals Transcriptomic and Anatomic Profiling Reveal Etiolation Promotes Adventitious Rooting by Exogenous Application of 1-Naphthalene Acetic Acid in Robinia pseudoacacia L.

Forests ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 789
Author(s):  
Muhammad Zeeshan Munir ◽  
Saleem Ud Din ◽  
Muhammad Imran ◽  
Zijie Zhang ◽  
Tariq Pervaiz ◽  
...  
Keyword(s):  
Adventitious Root ◽  
Woody Plants ◽  
Root Formation ◽  
Robinia Pseudoacacia ◽  
Adventitious Rooting ◽  
Adventitious Root Formation ◽  
Naphthalene Acetic Acid ◽  
Exogenous Application ◽  
Phenylpropanoid Biosynthesis ◽  
Robinia Pseudoacacia L

The process of etiolation contributes significantly to vegetative propagation and root formation of woody plants. However, the molecular interaction pattern of different factors for etiolated adventitious root development in woody plants remains unclear. In the present study, we explored the changes at different etiolation stages of adventitious root formation in Robinia pseudoacacia. Histological and transcriptomic analyses were performed for the etiolated lower portion of hypocotyls to ascertain the adventitious root responses. We found that the dark-treated hypocotyls formed roots earlier than the control. Exogenous application of NAA (0.3 mg/L) stimulated the expressions of about 310 genes. Among these, 155 were upregulated and 155 were downregulated. Moreover, differentially expressed genes (DEGs) were significantly enriched in multiple pathways, including the biosynthesis of secondary metabolites, metabolic pathway, plant hormone signal transduction, starch and sucrose metabolism, phenylpropanoid biosynthesis, and carbon metabolism. These pathways could play a significant role during adventitious root formation in etiolated hypocotyls. The findings of this study can provide novel insights and a foundation for further studies to elucidate the connection between etiolation and adventitious root formation in woody plants.

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