scholarly journals The Effect of Single and Combined Use of Gamma Radiation and Ethylmethane Sulfonate on Early Growth Parameters in Sorghum

Plants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 827 ◽  
Author(s):  
Maliata Athon Wanga ◽  
Hussein Shimelis ◽  
Lydia N. Horn ◽  
Fatma Sarsu
Keyword(s):  
Genetic Variation ◽  
Gamma Radiation ◽  
Growth Parameters ◽  
Seedling Survival ◽  
Seedling Emergence ◽  
Mutation Breeding ◽  
Dose Rates ◽  
Combined Use ◽  
Ethylmethane Sulfonate ◽  
Sorghum Genotypes

Success in inducing genetic variation through mutagenic agents is dependent on the source and dose of application. The objective of this study was to determine the optimum doses of a single and combined use of gamma radiation and ethylmethane sulfonate (EMS) for effective mutation breeding in sorghum. The study involved two concurrent experiments as follows: in experiment I, the seeds of four sorghum genotypes (‘Parbhani Moti’, ‘Parbhani Shakti’, ‘ICSV 15013′, and ‘Macia’) were treated using gamma radiation (0, 300, 400, 500 and 600 Gy), EMS (0, 0.5 and 1.0%), and gamma radiation followed by EMS (0 and 300 Gy and 0.1% EMS; 400 Gy and 0.05% EMS). In experiment II, the seeds of two genotypes (‘Macia’ and ‘Red sorghum’) were treated with seven doses of gamma radiation only (0, 100, 200, 300, 400, 500 and 600 Gy). Overall, the combined applied doses of gamma radiation and EMS are not recommended due to poor seedling emergence and seedling survival rate below LD50. The best dosage of gamma radiation for genotypes Red sorghum, Parbhani Moti, Macia, ICSV 15013 and Parbhani Shakti ranged between 392 and 419 Gy, 311 and 354 Gy, 256 and 355 Gy, 273 and 304 Gy, and 266 and 297 Gy, respectively. The EMS optimum dosage ranges for genotypes Parbhani Shakti, ICSV 15013, Parbhani Moti and Macia were between 0.41% and 0.60%, 0.48% and 0.58%, 0.46% and 0.51%, and 0.36% and 0.45%, respectively. The above dose rates are useful to induce genetic variation in the tested sorghum genotypes for greater mutation events in sorghum breeding programs.

Lethal Dose (LD50) Fixation and Sensitivity of Fenugreek (Trigonellafoenum-graecumL.) to Gamma Radiation for Induction of Mutation

2017 ◽  
Vol 4 (04) ◽  
Author(s):  
ANURADHA PATEL ◽  
POONAM VERMA ◽  
SHARDA CHOUDHARY ◽  
ARVIND KUMAR VERMA
Keyword(s):  
Genetic Variability ◽  
Gamma Rays ◽  
Growth Parameters ◽  
Seedling Survival ◽  
Lethal Dose ◽  
Germination Percentage ◽  
Mutation Breeding ◽  
Vigour Index ◽  
Small Flower ◽  
Dose Dependent

Fenugreek (Trigonella foenum-graecumL.) is an annual crop, mainly used as a spiceand leafy vegetable crop in many parts of the world. Classical breeding in fenugreek is restricted due to its low genetic variability and small flower size which hamper manual emasculation and pollination. Mutation breeding is an effective way to enrich genetic variability in crop plants. An experiment was conducted to determine the lethal dose of the physical mutagen gamma rays in fenugreek. The dry seeds of fenugreek were exposed to different doses of gamma rays i.e. 150Gy, 200Gy, 250Gy, 300Gy and 350Gy. These irradiated seeds were sown in the Petri plates with non-irradiated seeds (control). As the dose of gamma rays increased, there was a decrease in germination percentage, seedling survival, root length, shoot length and vigour index. Among five doses of gamma rays, the maximum seed germination was observed at lowest dose 150Gy (93%), followed by 200Gy (83%), 250Gy (76%), 300Gy (76%) and 350Gy (64%). The seedling survival was decreased from 90% (in control) to 56% in 350Gy dose of gamma rays. The gamma rays dose of 150Gy gave stimulatory effect on seedlings growth. The growth parameters were dose dependent, as the dose of gamma rays increased from 200Gy to 350Gy. The gamma rays dose of 350Gy showed 64% seeds germination and 56% of seedlings survival. Therefore, it is concluded that the LD50 dose for fenugreek is close to 350Gy. This information would be highly useful for initiating mutation breeding programme in fenugreek

scholarly journals Effects of High Soil Surface Temperature on Seedling Survival in Pearl Millet

1993 ◽  
Vol 29 (2) ◽  
pp. 215-225 ◽  
Author(s):  
J. M. Peacock ◽  
P. Soman ◽  
R. Jayachandran ◽  
A. U. Rani ◽  
C. J. Howarth ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Pearl Millet ◽  
Seedling Survival ◽  
Seedling Emergence ◽  
Soil Surface ◽  
Soil Moisture Deficit ◽  
High Soil ◽  
Moisture Deficit ◽  
Seedling Emergence And Survival ◽  
Soil Surface Temperature

SummaryA field technique to screen pearl millet genotypes for their emergence and survival at high soil surface temperatures is described. Genetic variation in seedling emergence and survival is shown and it is argued that this variation is largely due to tolerance of high temperatures rather than tolerance of soil moisture deficit, although some interaction occurred. An index for ‘thermotolerance’ is defined and genotypes are ranked accordingly for this trait, which is shown to be highly heritable. The technique is repeatable and allows a large number of genotypes to be screened at the same time.

Genetic variation for rate of establishment in caucasian clover

1998 ◽  
pp. 213-217
Author(s):  
K.H. Widdup ◽  
T.L. Knight ◽  
C.J. Waters
Keyword(s):  
Genetic Variation ◽  
Root Growth ◽  
Seedling Growth ◽  
White Clover ◽  
Seedling Emergence ◽  
Red Clover ◽  
Dry Weight ◽  
Cool Season ◽  
Trifolium Ambiguum ◽  
Root Dry Weight

Slow establishment of caucasian clover (Trifolium ambiguum L.) is hindering the use of this legume in pasture mixtures. Improved genetic material is one strategy of correcting the problem. Newly harvested seed of hexaploid caucasian clover germplasm covering a range of origins, together with white and red clover and lucerne, were sown in 1 m rows in a Wakanui soil at Lincoln in November 1995. After 21 days, the caucasian clover material as a group had similar numbers of emerged seedlings as white clover and lucerne, but was inferior to red clover. There was wide variation among caucasian clover lines (48-70% seedling emergence), with the cool-season selection from cv. Monaro ranked the highest. Recurrent selection at low temperatures could be used to select material with improved rates of seedling emergence. Red clover and lucerne seedlings produced significantly greater shoot and root dry weight than caucasian and white clover seedlings. Initially, caucasian clover seedlings partitioned 1:1 shoot to root dry weight compared with 3:1 for white clover. After 2 months, caucasian clover seedlings had similar shoot growth but 3 times the root growth of white clover. Between 2 and 5 months, caucasian clover partitioned more to root and rhizome growth, resulting in a 0.3:1 shoot:root ratio compared with 2:1 for white clover. Both clover species had similar total dry weight after 5 months. Unhindered root/ rhizome devel-opment is very important to hasten the establishment phase of caucasian clover. The caucasian clover lines KZ3 and cool-season, both selections from Monaro, developed seedlings with greater shoot and root growth than cv. Monaro. KZ3 continued to produce greater root growth after 5 months, indicating the genetic potential for improvement in seedling growth rate. Different pasture estab-lishment techniques are proposed that take account of the seedling growth characteristics of caucasian clover. Keywords: establishment, genetic variation, growth, seedling emergence, Trifolium ambiguum

Competitive attributes ofA. sativa, T. aestivum, andH. vulgareare conserved in no-till cropping systems

Weed Science ◽  
1999 ◽  
Vol 47 (6) ◽  
pp. 712-719 ◽  
Author(s):  
Anne Légère ◽  
Yuguang Bai
Keyword(s):  
Hordeum Vulgare ◽  
Avena Sativa ◽  
Growth Parameters ◽  
Cropping Systems ◽  
Seedling Emergence ◽  
Biomass Accumulation ◽  
Tillage Systems ◽  
Area Index ◽  
No Till ◽  
Lower Test

The robustness of competitive attributes of cereals such as rapid and uniform seedling emergence, tillering, early biomass accumulation and canopy closure, and height advantage over weeds have not yet been tested under environmental conditions typical of no-till (NT) cropping systems. Our objective was to evaluate the effects or NT practices on growth and productivity ofAvena sativa, Triticum aestivum, Hordeum vulgare, and associated weeds. The experiment was conducted on a Kamouraska clay at La Pocatière, QC, in 1994, 1995, and 1996.Avena sativa, T. aestivum, andH. vulgarewere grown under tilled and NT practices. Cereal growth parameters were measured six (1994) or seven (1995) times between planting and the 11th week after planting but only once in 1996. Grain yields and yield components were determined at crop maturity.Avena sativaandH. vulgarepopulations were little affected by tillage, whereasT. aestivumpopulations were reduced by 16 to 20% in NT systems. Growth in height in NT systems was either similar or greater than in tilled systems in all three cereals. Cereal leaf area index (LAI) and biomass accumulation was also comparable between tillage systems, except forT. aestivumLAI in 1994, which was greater in tilled plots on two sampling dates. Response of annual dicots to tillage was inconsistent in all crops. Annual monocots dominated in some but not all NT systems. Perennial dicots dominated in NT systems, whereas perennial monocots were more abundant in tilled systems in all three cereals.Avena sativaandT. aestivumyields in NT plots were comparable or greater than in tilled plots, in spite of having either lower test weights (A. sativa) or lower 1,000-grain weights (T. aestivum). NTT. aestivumproductivity was maintained in spite of reduced plant establishment.Hordeum vulgareyields were also similar across tillage systems, except in 1995, when yields in tilled plots were greater than in NT plots. The height advantage observed for NTH. vulgaredid not result in improved yields. All three cereals, and particularlyA. sativa, appeared well suited to NT systems, despite the pressure provided by different weed groups, compared to tilled systems. However, results suggest that NT production of cereals could benefit from improved attention to perennial dicot control and crop seedling establishment, particularly forT. aestivum.

Bigleaf maple seedling establishment and early growth in Douglas-fir forests

1988 ◽  
Vol 18 (10) ◽  
pp. 1226-1233 ◽  
Author(s):  
Jeremy S. Fried ◽  
John C. Tappeiner II ◽  
David E. Hibbs
Keyword(s):  
Seed Predation ◽  
Seedling Survival ◽  
Seedling Emergence ◽  
Early Growth ◽  
Douglas Fir ◽  
Stand Development ◽  
Size Distributions ◽  
Canopy Density ◽  
Successful Establishment ◽  
Survival Of Seedlings

Survival, age and height distributions, and stocking of bigleaf maple (Acermacrophyllum Pursh) seedlings were studied in 1- to 250-year-old Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) stands in western Oregon to identify the stages in stand development in which bigleaf maple is most likely to establish successfully from seed. Maple seedling emergence averaged 30–40% where seeds were planted and protected from rodents but was typically <2% for unprotected seeds. Seedling survival after 2 years was highly dependent on canopy density, measured by percent sky. Average 1st-year survival of seedlings originating from planted, protected seeds was highest in clearcuts (1–2 years old, 36% survival, 56% sky) and pole-size stands (41–80 years old, 30% survival, 17% sky) with sparse understories and canopies. It was lowest in young stands with dense canopies (20–40 years old, 4% survival, 8% sky) and old stands (81–250 years old, 14% survival, 13% sky) with dense understories. Naturally regenerated populations of bigleaf maple seedlings, which occurred in aggregations (0.005–0.04 ha in area), were most abundant (up to 10 000/ha) in pole-size Douglas-fir stands. Although seedling size distributions within stands had a strongly inverse J shaped form, size distributions within aggregations appeared more normal (bell-shaped). Seedling age rarely exceeded 15 years. Seedlings grew slowly in the understory, often reaching only 25 cm in height after 8–10 years, and were intensively browsed by deer. Naturally regenerated seedlings were virtually absent from clearcuts, probably because of dense competing vegetation and lack of seed caused by poor dispersal and seed predation. The "window" for the most successful establishment of bigleaf maple seedlings appears to begin after canopy thinning and end before forbs and shrubs invade.

Factors influencing germination of Scolochloa festucacea caryopses

1972 ◽  
Vol 50 (11) ◽  
pp. 2085-2092 ◽  
Author(s):  
Alan L. Smith
Keyword(s):  
Ethyl Alcohol ◽  
Seedling Growth ◽  
Seedling Survival ◽  
Seedling Emergence ◽  
Anaerobic Conditions ◽  
Factors Influencing

Germination and seedling emergence increased with increasing caryopses weight in Scolochloa festucacea. Maximum seedling survival occurred when caryopses were planted 1 cm deep. Simulation of anaerobic conditions by soaking caryopses increased seedling emergence. Seedling emergence increased after imbibed caryopses were treated in a N2 atmosphere. Emergence also was increased by soaking caryopses in 95% ethyl alcohol up to 60 min. Stratification apparently improved germination but was not a requirement. Scolochloa festucacea caryopses were more tolerant of MgCl2 than NaCl in the germination media. Optimum seedling growth occurred at 20C.

Direct seeding of PinusSylvestris using microsite preparation and invigorated seed lots of different quality: 2-year results

1994 ◽  
Vol 24 (1) ◽  
pp. 77-86 ◽  
Author(s):  
Hans Winsa ◽  
Urban Bergsten
Keyword(s):  
Seed Quality ◽  
Seedling Survival ◽  
Seedling Emergence ◽  
Mineral Soil ◽  
Direct Seeding ◽  
Growing Seasons ◽  
Humus Layer ◽  
Negative Effect ◽  
Mean Germination Time ◽  
Better Than

Direct seeding of Pinussylvestris L. is a regeneration method, with potential for development considering scarification, microsite preparation, seed invigoration, and seed quality. Three seed lots of different quality concerning seed weight, germination percent, and mean germination time were used on two sites in northern Sweden. Microsite preparation, 2 cm deep pyramidal indentations, of the mineral soil improved seedling emergence on the two sites by 48 and 62%, respectively, compared with seeding without preparation other than removal of the humus layer. Microsite preparation in combination with invigorated seed, i.e., seed incubated at 30% moisture content for 7 days at 15 °C, resulted in seedling emergence of about 85% for the highest and about 50% for the lowest seed quality at both sites. Noninvigorated seed, seeded without microsite preparation, reached about 55% for the highest and 22% at one and 43% at the other for the lowest seed quality. Without microsite preparation there was no, or a negative, effect of seed invigoration on seedling emergence. Seedling survival after the first winter improved significantly with better seed quality. Survival averaged 92 and 72% at the two sites, with frost heaving causing most mortality. Seedlings from invigorated and redried seed survived better than seedlings from untreated seed. Seedlings from the best seed quality had higher values in seedling height, about 35%, shoot length, about 60%, and needle length, about 30%, after two growing seasons than seedlings from lower seed qualities. Invigoration and microsite preparation had no effect on measured growth characteristics.

scholarly journals Differential expression of NPM, GSTA3, and GNMT in mouse liver following long-term in vivo irradiation by means of uranium tailings

2018 ◽  
Vol 38 (5) ◽  
Author(s):  
Lan Yi ◽  
Hongxiang Mu ◽  
Nan Hu ◽  
Jing Sun ◽  
Jie Yin ◽  
...  
Keyword(s):  
Gamma Radiation ◽  
Radiation Exposure ◽  
Central Vein ◽  
Glutathione S Transferase ◽  
Γ Radiation ◽  
Dose Rates ◽  
Living Organisms ◽  
Uranium Tailings

Uranium tailings (UT) are formed as a byproduct of uranium mining and are of potential risk to living organisms. In the present study, we sought to identify potential biomarkers associated with chronic exposure to low dose rate γ radiation originating from UT. We exposed C57BL/6J mice to 30, 100, or 250 μGy/h of gamma radiation originating from UT samples. Nine animals were included in each treatment group. We observed that the liver central vein was significantly enlarged in mice exposed to dose rates of 100 and 250 μGy/h, when compared with nonirradiated controls. Using proteomic techniques, we identified 18 proteins that were differentially expressed (by a factor of at least 2.5-fold) in exposed animals, when compared with controls. We chose glycine N-methyltransferase (GNMT), glutathione S-transferase A3 (GSTA3), and nucleophosmin (NPM) for further investigations. Our data showed that GNMT (at 100 and 250 μGy/h) and NPM (at 250 μGy/h) were up-regulated, and GSTA3 was down-regulated in all of the irradiated groups, indicating that their expression is modulated by chronic gamma radiation exposure. GNMT, GSTA3, and NPM may therefore prove useful as biomarkers of gamma radiation exposure associated with UT. The mechanisms underlying those changes need to be further studied.

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