scholarly journals Comparative Functional Analysis of Class II Potassium Transporters, SvHKT2;1, SvHKT2;2, and HvHKT2;1, on Ionic Transport and Salt Tolerance in Transgenic Arabidopsis

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 786
Author(s):  
Yuichi Tada ◽  
Aki Ohnuma
Keyword(s):  
Salt Tolerance ◽  
Transport Properties ◽  
Plant Cells ◽  
Ionic Transport ◽  
Class Ii ◽  
Phloem Sap ◽  
Over Expression ◽  
Potassium Transporters ◽  
Transgenic Lines ◽  
Reduced Salt

Class II high-affinity potassium transporters (HKT2s) mediate Na+–K+ cotransport and Na+/K+ homeostasis under K+-starved or saline conditions. Their functions have been studied in yeast and X. laevis oocytes; however, little is known about their respective properties in plant cells. In this study, we characterized the Na+ and K+ transport properties of SvHKT2;1, SvHKT2;2 and HvHKT2;1 in Arabidopsis under different ionic conditions. The differences were detected in shoot K+ accumulation and root K+ uptake under salt stress conditions, K+ accumulation in roots and phloem sap under K+-starved conditions, and shoot and root Na+ accumulation under K+-starved conditions among the HKT2s transgenic lines and WT plants. These results indicate the diverse ionic transport properties of these HKT2s in plant cells, which could not be detected using yeast or X. laevis oocytes. Furthermore, Arabidopsis expressing HKT2s showed reduced salt tolerance, while over-expression of HvHKT2;1 in barley, which has the ability to sequestrate Na+, showed enhanced salt tolerance by accumulating Na+ in the shoots. These results suggest that the coordinated enhancement of Na+ accumulation and sequestration mechanisms in shoots could be a promising strategy to confer salt tolerance to glycophytes.

scholarly journals Na+ Transporter SvHKT1;1 from a Halophytic Turf Grass Is Specifically Upregulated by High Na+ Concentration and Regulates Shoot Na+ Concentration

2020 ◽  
Vol 21 (17) ◽  
pp. 6100
Author(s):  
Yuki Kawakami ◽  
Shahin Imran ◽  
Maki Katsuhara ◽  
Yuichi Tada
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Early Stage ◽  
Constitutive Expression ◽  
Xenopus Laevis Oocytes ◽  
Na Transport ◽  
Turf Grass ◽  
Defective Mutant ◽  
Reduced Salt ◽  
Na Uptake

We characterized an Na+ transporter SvHKT1;1 from a halophytic turf grass, Sporobolus virginicus. SvHKT1;1 mediated inward and outward Na+ transport in Xenopus laevis oocytes and did not complement K+ transporter-defective mutant yeast. SvHKT1;1 did not complement athkt1;1 mutant Arabidopsis, suggesting its distinguishable function from other typical HKT1 transporters. The transcript was abundant in the shoots compared with the roots in S. virginicus and was upregulated by severe salt stress (500 mM NaCl), but not by lower stress. SvHKT1;1-expressing Arabidopsis lines showed higher shoot Na+ concentrations and lower salt tolerance than wild type (WT) plants under nonstress and salt stress conditions and showed higher Na+ uptake rate in roots at the early stage of salt treatment. These results suggested that constitutive expression of SvHKT1;1 enhanced Na+ uptake in root epidermal cells, followed by increased Na+ transport to shoots, which led to reduced salt tolerance. However, Na+ concentrations in phloem sap of the SvHKT1;1 lines were higher than those in WT plants under salt stress. Based on this result, together with the induction of the SvHKT1;1 transcription under high salinity stress, it was suggested that SvHKT1;1 plays a role in preventing excess shoot Na+ accumulation in S. virginicus.

Ion measurements by X-ray microanalysis in unfixed, frozen, hydrated plant cells of species differing in salt tolerance

Planta ◽  
1977 ◽  
Vol 134 (1) ◽  
pp. 35-38 ◽  
Author(s):  
A. R. Yeo ◽  
A. L�uchli ◽  
D. Kramer ◽  
J. Gullasch
Keyword(s):  
Salt Tolerance ◽  
Plant Cells ◽  
X Ray

scholarly journals Overexpression of a Vesicle Trafficking Gene, OsRab7, Enhances Salt Tolerance in Rice

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Xiaojue Peng ◽  
Xia Ding ◽  
Tianfang Chang ◽  
Zhoulong Wang ◽  
Rong Liu ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Transgenic Rice ◽  
Agricultural Production ◽  
Vesicle Trafficking ◽  
Yeast Cells ◽  
Root Tip ◽  
Over Expression ◽  
Crop Tolerance ◽  
Vacuolar Trafficking ◽  
Main Factor

High soils salinity is a main factor affecting agricultural production. Studying the function of salt-tolerance-related genes is essential to enhance crop tolerance to stress.Rab7is a small GTP-binding protein that is distributed widely among eukaryotes. Endocytic trafficking mediated byRab7plays an important role in animal and yeast cells, but the current understanding ofRab7in plants is still very limited. Herein, we isolated a vesicle trafficking gene,OsRab7, from rice. Transgenic rice over-expressingOsRab7exhibited enhanced seedling growth and increased proline content under salt-treated conditions. Moreover, an increased number of vesicles was observed in the root tip ofOsRab7transgenic rice. TheOsRab7over-expression plants showed enhanced tolerance to salt stress, suggesting that vacuolar trafficking is important for salt tolerance in plants.

Over-expression of the rice OsAMT1-1 gene increases ammonium uptake and content, but impairs growth and development of plants under high ammonium nutrition

2006 ◽  
Vol 33 (2) ◽  
pp. 153 ◽  
Author(s):  
Mohammad S. Hoque ◽  
Josette Masle ◽  
Michael K. Udvardi ◽  
Peter R. Ryan ◽  
Narayana M. Upadhyaya
Keyword(s):  
Transgenic Plants ◽  
Ammonium Assimilation ◽  
Ammonium Transporter ◽  
Ammonium Uptake ◽  
Vegetative Stage ◽  
Over Expression ◽  
Transgenic Lines ◽  
Ammonium Nutrition ◽  
Maize Ubiquitin Promoter

A transgenic approach was undertaken to investigate the role of a rice ammonium transporter (OsAMT1-1) in ammonium uptake and consequent ammonium assimilation under different nitrogen regimes. Transgenic lines overexpressing OsAMT1-1 were produced by Agrobacterium-mediated transformation of two rice cultivars, Taipei 309 and Jarrah, with an OsAMT1-1 cDNA gene construct driven by the maize ubiquitin promoter. Transcript levels of OsAMT1-1 in both Taipei 309 and Jarrah transgenic lines correlated positively with transgene copy number. Shoot and root biomass of some transgenic lines decreased during seedling and early vegetative stage compared to the wild type, especially when grown under high (2 mm) ammonium nutrition. Transgenic plants, particularly those of cv. Jarrah recovered in the mid-vegetative stage under high ammonium nutrition. Roots of the transgenic plants showed increased ammonium uptake and ammonium content. We conclude that the decreased biomass of the transgenic lines at early stages of growth might be caused by the accumulation of ammonium in the roots owing to the inability of ammonium assimilation to match the greater ammonium uptake.

On the electronic and ionic transport properties of La2Mo2O9

2003 ◽  
Vol 38 (15) ◽  
pp. 1965-1977 ◽  
Author(s):  
Raghavan Subasri ◽  
Helfried Näfe ◽  
Fritz Aldinger
Keyword(s):  
Transport Properties ◽  
Ionic Transport

Over-expression of a plasma membrane H+-ATPase SpAHA1 conferred salt tolerance to transgenic Arabidopsis

PROTOPLASMA ◽  
2018 ◽  
Vol 255 (6) ◽  
pp. 1827-1837 ◽  
Author(s):  
Yafei Fan ◽  
Shumin Wan ◽  
Yingshuo Jiang ◽  
Youquan Xia ◽  
Xiaohui Chen ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Salt Tolerance ◽  
Transgenic Arabidopsis ◽  
Over Expression

Rheological and Ionic Transport Properties of Nanocomposite Electrolytes Based on Protic Ionic Liquids and Silica Nanoparticles

Langmuir ◽  
2019 ◽  
Vol 36 (1) ◽  
pp. 148-158 ◽  
Author(s):  
Mayeesha Marium ◽  
Mahfuzul Hoque ◽  
Muhammed Shah Miran ◽  
Morgan L. Thomas ◽  
Izuru Kawamura ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Transport Properties ◽  
Silica Nanoparticles ◽  
Ionic Transport ◽  
Protic Ionic Liquids ◽  
Nanocomposite Electrolytes
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