scholarly journals Tolerance of Douglas Fir Somatic Plantlets to Aluminum Stress: Biological, Cytological, and Mineral Studies

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 536
Author(s):  
Holm Amara ◽  
Marie-Anne Lelu-Walter ◽  
Vincent Gloaguen ◽  
Céline Faugeron-Girard
Keyword(s):  
Growth And Development ◽  
Culture Medium ◽  
Dry Mass ◽  
Douglas Fir ◽  
Aluminum Stress ◽  
In Vitro Plantlets ◽  
Significant Damage ◽  
Root Tissues ◽  
Shed Light

Aluminum (Al) is well known as a potent inhibitor of plant growth and development. It is notably present in soils in the soluble and bioavailable form Al3+ when the soil pH drops below 5. This situation is frequent, especially in softwood forests when litter decomposition is slow. In the present work, we studied the effects of Al3+ on the growth and development of Douglas fir plantlets. Somatic plantlets, regenerated via somatic embryogenesis, were grown in vitro on media supplemented with different concentrations of aluminum chloride (AlCl3): 0 µM, 200 µM, 500 µM. and 1 mM. We show that a concentration of 500 µM AlCl3 in medium significantly reduced root elongation (−21.8%), as well as stem growth (−14.6%). Also, a 25% reduction in dry mass of the plantlets was observed in presence of a concentration of 200 µM of AlCl3. Histological analysis of root tissues revealed significant damage, especially in conducting vessels. In addition, mineral cation content of plantlets was disturbed under Al exposure. More particularly, the Mg and K contents of needles and the Ca content of stems and needles were significantly reduced in presence of a concentration of 500 µM AlCl3 in the culture medium (−35.6%, −33.5%, −24%, and −34% respectively). However, all these damages appeared at relatively high Al concentrations when compared with other herbaceous species. This study shed light on the ability of Douglas fir in vitro plantlets to cope with the acid-driven toxicity of Al.

scholarly journals Monitoring the end of the in vitro phase of Anthurium andreanum Lindl. plantlets

2010 ◽  
Vol 22 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Giulio Cesare Stancato ◽  
Maria Luiza Sant’Anna Tucci
Keyword(s):  
Culture Medium ◽  
Soluble Sugars ◽  
Dry Mass ◽  
Total Soluble Sugars ◽  
Gas Exchanges ◽  
Single Action ◽  
Fluorescent Lamps ◽  
Anthurium Andreanum

Estimulation of autotrophy in in vitro plantlets could be achieved through changes in the culture medium, or by changing the traditional hermetic caps by one that could allow gas exchanges between the culture and the environment. Besides that, the use of lamps with distinct emission spectrum irradiaction has propitiated successful results. This work was carried out aiming to evaluate the either the combined or the single action of some factors that can induce autotrophy on in vitro A. andraeanum cv. Eidibel plantlets. 3 sucrose concentrations were used: 0, 15 and 60 mM and for each one, to kinds of flasks according to the cap ventilation: under (0.038 L.h-1) and without ventilation. Flasks were kept under cold light fluorescent lamps or under gro-lux lamps. At the end of the experiment showing the highest shoot dry mass treatment was 60 mM, under ventilation and gro-lux, and the treatment which accumulate root dry mass to a lesser extent were 0 mM with ventilation and cold light and 15 mM without ventilation and cold light. In average, treatments with higher sucrose content in the culture medium, that is, 60 mM, under gro-lux lamps, presented the highest chlorophyll a, b and total contents, than those under cold lamp. Steps of carbohydrates metabolism could be associated with the total soluble sugars (sucrose and reducing sugars) levels, highlighting the steps where nutrient requirements were higher, showing the role of the plantlets sink.

scholarly journals The creation of a Taraxacum kok-saghyz high frequency regeneration system

2021 ◽  
Vol 843 (1) ◽  
pp. 012041
Author(s):  
A A Verbitskaya ◽  
A S Ivanova ◽  
N G Konkova ◽  
A K Gaponenko
Keyword(s):  
Culture Medium ◽  
Ph Value ◽  
Culture Media ◽  
Shoot Formation ◽  
High Survival ◽  
Regeneration System ◽  
Hormone Exposure ◽  
Direct Shoot Formation ◽  
Root Tissues

Abstract The aim of this research was to study the morphogenetic ability of Taraxacum kok-saghyz root tissues and to optimize the culture medium for the subsequent genetic transformation of plants. The effects of exogenous hormone exposure on survival, in vitro shoot induction, and root formation were studied by using root tissues. For the cultivation the samples of kok-saghyz, the Murashige-Skuga nutrient medium was used as the basis, supplemented with sucrose 20 g/l, vitamins B5 1 mg/l, and also containing agar 5.5 g/l. The pH value is 5.7. For the plant regeneration induction, growth regulators, auxins and cytokinins were added in culture media. In this study, the roots of D 1 mm and two media variants were used for comparison: variant 1. MS + 6-BAP 1 mg/l; variant 2. MS + 6-BAP 1 mg/l + IAA 0.2 mg/l. An effective protocol for the regeneration of kok-saghyz explants was developed. There was a high percentage of regeneration of 87.6% on the medium containing a combination of cytokinin and auxin, as well as a high percentage of direct shoot formation, which was 65.1%, the degree of rooting was 100%, the resulting cultured plant tissues grew well and had a high survival rate after transplantation.

scholarly journals Concentrations of cytokinin and the use of agricultural residues in the in vitro propagation media of highbush blueberry

2020 ◽  
Vol 50 (6) ◽  
Author(s):  
Michele Carla Nadal ◽  
Adriane Marinho de Assis ◽  
Dianini Brum Frölech ◽  
Bruna Andressa dos Santos Oliveira ◽  
Cari Rejane Fiss Timm ◽  
...  
Keyword(s):  
Rice Husk ◽  
In Vitro Propagation ◽  
Culture Medium ◽  
Propagation Rate ◽  
Dry Mass ◽  
Vaccinium Corymbosum ◽  
Agricultural Residues ◽  
Highbush Blueberry ◽  
Rice Husks

ABSTRACT: The objective of the research was to evaluate cytokinin concentrations and agricultural residues as physical structuring agents of the culture medium in the in vitro propagation of highbush blueberry (Vaccinium corymbosum ‘Duke’). To that end, three different concentrations of 2-isopentenyladenine (2iP) (0, 5, and 10 mg L-1)in woody plant medium (WPM) and five structuring agents (in natura rice husks, carbonized rice husks, coconut fiber, S-10 Beifort®, and agar)were tested. After 60days of culture, the following parameters were evaluated: survival (%),sprout and shoot lengths (cm),propagation rate, number of sprouts, and shoot dry mass (g). The substrates used as structuring agents were analyzed in terms of pH and electrical conductivity. The in natura rice husk, S-10 Beifort®, and carbonized rice husk did not differ from the agar in terms of the sprout length. The shoots were longer (1.64 cm) in cultures within natura rice husk than in other treatments. In the absence of 2iP, S-10 Beifort® resulted in the highest propagation rate (2.97). Concerning the number of sprouts, S-10 Beifort®, in natura rice husks, and carbonized rice husks did not differ from agar. However, when the regulator was added, the agar performed better in comparison to the other residues. Thus, in natura rice husks, carbonized rice husks, and S-10 Beifort®are potential physical structuring agents of the culture medium that can be used without 2iP.

scholarly journals THE OPTIMIZATION OF JELLIFYING AGENT COMPOSITION ON REGENERATION OF IN VITRO OBTAINED POTATO MICROPLANTS

2016 ◽  
Vol 24 ◽  
pp. 79-82
Author(s):  
H. M. Shevaha ◽  
M. M. Kyryk ◽  
V. M. Hunchak ◽  
T. M. Oliinyk
Keyword(s):  
Growth And Development ◽  
Culture Medium ◽  
In Vitro Growth ◽  
Maize Starch ◽  
Potato Plants

Results of studies on the optimization of Murashige and Skoog culture medium withmodified maize starch for the acceleration of potato plants micropropogation are provided. Ithas been established that the method modification increases surveillance parameters, promotesactive in vitro growth and development of potato springs.

scholarly journals Organic fertilizer on the in vitro cultivation of the Cattleya labiata orchid

2017 ◽  
Vol 2 (2) ◽  
pp. 62
Author(s):  
Rodrigo Thibes Hoshino ◽  
Ronan Carlos Colombo ◽  
Ana Paula Zandoná ◽  
Guilherme Augusto Cito Alves ◽  
Ricardo Tadeu de Faria
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Average Length ◽  
Organic Fertilizer ◽  
Dry Mass ◽  
In Vitro Cultivation ◽  
Shoot Height ◽  
Organic Products ◽  
Number Of Leaves

Propagation of in vitro plants through other culture media rather than the traditional ones has been widely researched, with satisfactory results. However, to increase the effectiveness of these media, the addition of organic products has been presented satisfactory results. Therefore, the objective of this work was to evaluate the influence of the FishFértil® organic fertilizer on the in vitro cultivation of the Brazilian orchid Cattleya labiata. Treatments included FishFértil® fertilizer concentrations of 0; 1; 2; 3; 4; 5 and 6 mL L-1 in a simplified culture medium. At 180 days, shoot height, number of leaves, leaf area, number of roots, root average length, shoot and root dry mass and the shoot: root ratio were evaluated. The experimental design was entirely randomized, with 10 replications, each one containing 10 plantules. Data were submitted to an analysis of variance and regression analysis, at 5% of significance. The FishFértil® organic fertilizer at the concentration of 6 mL L-1 promoted better plantule growth of Cattleya labiata, subcultivated in vitro, in a simplified culture medium.

scholarly journals Effect of agar concentration in culture medium on in vitro development of potato plants

2020 ◽  
Vol 9 (7) ◽  
pp. e542974571
Author(s):  
Cinthia Kutz de Matos ◽  
Camila Eduarda Loli Pereira ◽  
Leonardo Balena ◽  
Jackson Kawakami
Keyword(s):  
Culture Medium ◽  
Agar Medium ◽  
Dry Mass ◽  
Culture Technique ◽  
Potato Cultivars ◽  
Semisolid Medium ◽  
Potato Plants ◽  
Best Response ◽  
Vitro Development

In vitro propagation and tissue culture technique is an alternative for obtaining healthy potato seeds. However, data found in the literature differ on the optimal consistency of the culture medium to be used in vitro. The aim in this research was to look at the adaptation of potato cultivars to medium with different agar concentrations. The experimental design was entirely randomized in a factorial scheme. The experiment tested the effect of 3 agar medium concentrations (solid, semisolid and liquid) on 6 cultivars (Agata, Bel, Camila, Catucha, Clara and Eliza), with 5 repetitions. We quantified from the plantlets, fresh and dry mass of roots and shoot, the number of nodes, the multiplication capacity and vigor. The study showed that potato cultivars respond significantly to different concentrations of agar and their best response was in the semisolid medium.

scholarly journals In vitro propagation of lemon verbena: a plant native of South America

2019 ◽  
Vol 41 ◽  
pp. e47105
Author(s):  
Marcos Vinícius Marques Pinheiro ◽  
Daniele Cristina Fontana ◽  
Jullie dos Santos ◽  
Matheus Milani Pretto ◽  
Gabrieli Cristina Vitalli de Azevedo ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Culture Medium ◽  
Culture Media ◽  
In Vitro Regeneration ◽  
Dry Mass ◽  
Full Development ◽  
Growing Conditions ◽  
Aloysia Triphylla ◽  
Lemon Verbena

In vitro propagation increases the supply and commercialisation of products of interest. For this, optimising the growing conditions and the composition of the culture medium is crucial to benefit the full development of the plants. Thus, the objective was to evaluate the in vitro propagation of Aloysia triphylla on different culture media, with varying agar and sucrose concentrations. The experiment was conducted as a completely randomised design, 3×3×3 factorial scheme, with three culture media (MS, JADS and WPM), three sucrose concentrations (8, 10 and 12 g L-1) and three agar concentrations (15, 30 and 45 g L-1), with three replicates each and experimental units composed of one plant per replicate. After 25 days of cultivation, the fresh and dry mass of the plants, numbers of leaves, numbers of nodes, plant lengths, numbers of oxidised leaves, hyperhydricity and acclimatization percentages were evaluated. The WPM medium resulted in a reduced fresh mass, reflecting in the low hyperhydricity observed in the explants, and favoured the acclimatization of the plants. Thus, the WPM medium with sucrose (15 g L-1) and agar (12 g L-1) is recommended as the medium most suitable for the in vitro regeneration of Aloysia triphylla.

scholarly journals Cattleya walkeriana growth in different micropropagation systems

2013 ◽  
Vol 43 (10) ◽  
pp. 1804-1810 ◽  
Author(s):  
André Luís Moreira ◽  
Adriano Bortolotti da Silva ◽  
Aline Santos ◽  
Caroline Oliveira dos Reis ◽  
Paulo Roberto Correa Landgraf
Keyword(s):  
Aerial Part ◽  
Natural Ventilation ◽  
Culture Medium ◽  
Culture Liquid ◽  
Dry Mass ◽  
Culture Vessel ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Conventional Systems

The aim of the present research was to verify the in vitro growth of orchids in different systems of micropropagation, being cultivated in a bioreactor, with natural ventilation and conventional systems. Cattleya walkeriana plants were obtained from the germination of seeds in culture medium. After 8 months, seedlings with 1 cm of length were placed in a culture vessel according to the treatments, which counted with two micropropagation systems (conventional and natural ventilation) in three media of culture (liquid, solid with 5 or 6g L-1 of agar). Two additional treatments in bioreactor of temporary and continuous immersion were performed. The design was entirely randomized (ERD), consisting of a 2x3 factorial with two additional treatments, totaling 8 treatments with three repetitions. The temporary immersion bioreactor promoted a bigger growth of the aerial part and of the root system, bigger accumulation of dry mass and better control of water loss by the plants. The temporary immersion bioreactor is the best micropropagation system for the C. walkeriana growth in vitro.

scholarly journals Asymbiotic seed germination and in vitro propagation of Cattleya trianae Linden & Reichb.f. (Orchidaceae)

2017 ◽  
Vol 66 (4) ◽  
pp. 544-548 ◽  
Author(s):  
Seir Antonio Salazar Mercado ◽  
Nelson Alfonso Vega Contreras
Keyword(s):  
Seed Germination ◽  
Threatened Species ◽  
Growth And Development ◽  
In Vitro Propagation ◽  
Culture Medium ◽  
Ms Medium ◽  
Organic Additives ◽  
Natural Habitats ◽  
Asymbiotic Seed Germination

Cattleya trianae (Linden & Reichb.f., 1860), Colombian national flower, is in danger of extinction due to the destruction of its natural habitats and excessive collection for horticultural purposes. Therefore, in vitro culture is a tool for the conservation of threatened species. In this study we determined the most suitable culture medium for asimbytic seed germination and in vitro propagation of C. trianae. Initially, mature capsules were collected, the seeds were subsequently disinfected and seeded with the syringe method (Vendrame et al., 2007), to evaluate the effect of five media on the development of C. trianae after 20 weeks. The seedlings were transplanted and acclimated using different substrates. The best percentage (54.2%) of seedling formation after 20 weeks was found in MS + JP medium with significant differences (P <0.05: Tukey HSD). In this research, it is reported that the addition of organic additives to the MS medium improves the efficacy of this, and therefore, allows a greater growth and development of C. trianae under in vitro conditions.

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