scholarly journals Effects of NtSPS1 Overexpression on Solanesol Content, Plant Growth, Photosynthesis, and Metabolome of Nicotiana tabacum

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 518
Author(s):  
Ning Yan ◽  
Xiaolei Gai ◽  
Lin Xue ◽  
Yongmei Du ◽  
John Shi ◽  
...  
Keyword(s):  
Chlorophyll Fluorescence ◽  
Nicotiana Tabacum ◽  
Plant Growth ◽  
Chlorophyll Content ◽  
Carbon Fixation ◽  
Leaf Growth ◽  
Pathway Enrichment Analysis ◽  
Photosynthetic Gas Exchange ◽  
Chlorophyll Fluorescence Parameters ◽  
Fluorescence Parameters

Nicotiana tabacum solanesyl diphosphate synthase 1 (NtSPS1) is the key enzyme in solanesol biosynthesis. However, changes in the solanesol content, plant growth, photosynthesis, and metabolome of tobacco plants after NtSPS1 overexpression (OE) have not been previously reported. In the present study, these parameters, as well as photosynthetic gas exchange, chlorophyll content, and chlorophyll fluorescence parameters, were compared between NtSPS1 OE and wild type (WT) lines of tobacco. As expected, NtSPS1 OE significantly increased solanesol content in tobacco leaves. Although NtSPS1 OE significantly increased leaf growth, photosynthesis, and chlorophyll content, the chlorophyll fluorescence parameters in the leaves of the NtSPS1 OE lines were only slightly higher than those in the WT leaves. Furthermore, NtSPS1 OE resulted in 64 differential metabolites, including 30 up-regulated and 34 down-regulated metabolites, between the OE and WT leaves. Pathway enrichment analysis of these differential metabolites identified differentially enriched pathways between the OE and WT leaves, e.g., carbon fixation in photosynthetic organisms. The maximum carboxylation rate of RuBisCO and the maximum rate of RuBP regeneration were also elevated in the NtSPS1 OE line. To our knowledge, this is the first study to confirm the role of NtSPS1 in solanesol biosynthesis and its possible functional mechanisms in tobacco.

scholarly journals Physiological, Biochemical and Chlorophyll Fluorescence Parameters of Physalis Peruviana L. Seedlings Exposed to Different Short-Term Waterlogging Periods and Fusarium Wilt Infection

Agronomy ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 213 ◽  
Author(s):  
Cristhian C. Chávez-Arias ◽  
Sandra Gómez-Caro ◽  
Hermann Restrepo-Díaz
Keyword(s):  
Chlorophyll Fluorescence ◽  
Plant Growth ◽  
Photosynthetic Pigments ◽  
Abiotic And Biotic Stresses ◽  
Chlorophyll Fluorescence Parameters ◽  
Biotic Stresses ◽  
Fluorescence Parameters ◽  
Physalis Peruviana ◽  
Progress Curve ◽  
Cape Gooseberry

Cape gooseberry has coped with abiotic and biotic stresses such as prolonged waterlogging periods and vascular wilt in recent years. The aim of this study was to evaluate the influence of four waterlogging periods on stomatal conductance (gs), leaf water potential (Ψwf), plant growth, leaf photosynthetic pigments, malondialdehyde (MDA) production, proline content and chlorophyll fluorescence parameters in cape gooseberry plants infected with Fusarium oxysporum f. sp. physali (Foph). Two-month-old ecotype “Colombia” plants were arranged in a completely randomized factorial design in eight treatments: plants without waterlogging (control), plants with waterlogging for 4, 6 and 8 d with and without Foph, respectively. The area under the disease progress curve was higher in inoculated plants subjected to 6 and 8 d of waterlogging (55.25 and 64.25) compared to inoculated plants but without waterlogging (45.25). The results also showed a lower plant growth, gs, Ψwf, leaf photosynthetic pigments and chlorophyll fluorescence parameters (Fv/Fm, electron transport rate (ETR), Y (II) and qP) as waterlogging periods in plants with Foph increased. However, this group of plants showed a greater proline and malondialdehyde (MDA) accumulation and a higher NPQ. In conclusion, cape gooseberry shows a low acclimation to waterlogging conditions of more than 6 d in soils with Foph.

scholarly journals Copper Stress on Photosynthesis of Black Mangle (Avicennia germinans)

2013 ◽  
Vol 85 (2) ◽  
pp. 665-670 ◽  
Author(s):  
DANIEL GONZALEZ-MENDOZA ◽  
FRANCISCO ESPADAS y GIL ◽  
FERNANDO ESCOBOZA-GARCIA ◽  
JORGE M. SANTAMARIA ◽  
OMAR ZAPATA-PEREZ
Keyword(s):  
Chlorophyll Fluorescence ◽  
Photosynthetic Apparatus ◽  
Copper Toxicity ◽  
Net Photosynthesis ◽  
Avicennia Germinans ◽  
Copper Stress ◽  
Photosynthetic Gas Exchange ◽  
Chlorophyll Fluorescence Parameters ◽  
Fluorescence Parameters ◽  
Photosynthetic Activities

The effects of copper toxicity on the photosynthetic activities of Avicennia germinans was investigated using two CuSO4 concentrations (0.062 and 0.33 M) added in Hoagland's solution in an aerated hydroponic system. Photosynthesis and chlorophyll fluorescence were measured after 30 h of copper stress. Results obtained in this study show that increasing levels of Cu+2 of 0.062 and 0.33 M Cu+2 resulted in a general reduction of the stomatal conductance (28 and 18%, respectively) and 100% of inhibition of net photosynthesis. Additionally, at these concentrations of Cu+2, reductions of chlorophyll fluorescence parameters were also observed. These changes suggested that the photosynthetic apparatus of Avicennia germinans was the primary target of the Cu+2 action. It is concluded that Cu+2 ions causes a drastic decline in photosynthetic gas exchange and Chlorophyll fluorescence parameters in A. germinans leaves.

Genetic variability and identification of quantitative trait loci affecting plant growth and chlorophyll fluorescence parameters in the model legume Medicago truncatula under control and salt stress conditions

2014 ◽  
Vol 41 (9) ◽  
pp. 983 ◽  
Author(s):  
Sarah Exbrayat ◽  
Georges Bertoni ◽  
Mohamad Reza Naghavie ◽  
Ali Peyghambari ◽  
Mounavar Badri ◽  
...  
Keyword(s):  
Salt Stress ◽  
Chlorophyll Fluorescence ◽  
Quantitative Trait Loci ◽  
Genetic Variability ◽  
Plant Growth ◽  
Medicago Truncatula ◽  
Quantitative Trait ◽  
Chlorophyll Fluorescence Parameters ◽  
Model Legume ◽  
Fluorescence Parameters

Salinity is one of the major stresses that limits crop production worldwide and affects most physiological activities in plants. In order to study the genetic control of salt stress in the model legume Medicago truncatula Gaertn., an experiment was undertaken to determine the genetic variability and to identify quantitative trait loci (QTLs) controlling several traits related to plant growth and physiology in a population of recombinant inbred lines. Shoot and root DW, relative water content, leaf area, chlorophyll content, chlorophyll fluorescence parameters, and Na+ and K+ in shoots and roots were measured. The experiment was carried out with three replications. ANOVA showed a large genetic variation and transgressive segregation for the traits studied, suggesting putative complex tolerance mechanisms. A total of 21 QTLs were detected under control conditions and 19 QTLs were identified under 100 mm salt stress conditions, with three QTLs being common to both situations. The percentage of total phenotypic variance explained by the QTLs ranged from 4.6% to 23.01%. Overlapping QTLs for different traits were also observed, which enables us to discriminate independent traits from linked ones. The results should be helpful information for further functional analysis of salt tolerance in M. truncatula.

scholarly journals Differential responses of ribulose-1,5-bisphosphate carboxylase/oxygenase activities of two Vigna unguiculata cultivars to salt stress

2005 ◽  
Vol 17 (2) ◽  
pp. 207-212 ◽  
Author(s):  
Maria Erivalda Farias de Aragão ◽  
Marjorie Moreira Guedes ◽  
Maria de Lourdes Oliveira Otoch ◽  
Maria Izabel Florindo Guedes ◽  
Dirce Fernandes de Melo ◽  
...  
Keyword(s):  
Salt Stress ◽  
Chlorophyll Fluorescence ◽  
Chlorophyll Content ◽  
Vigna Unguiculata ◽  
Shoot Growth ◽  
Bisphosphate Carboxylase ◽  
Chlorophyll Fluorescence Parameters ◽  
Fluorescence Parameters ◽  
Root And Shoot Growth ◽  
Specific Activities

Vita 3 and Vita 5 are two Vigna unguiculata cultivars that differ in their capacities for survival in saline environments; Vita 3 is more tolerant and Vita 5 more sensitive. Both cultivars were submitted to salt stress with 0.1 M NaCl. After 8 days, root and shoot growth from both cultivars was reduced but reduction was more pronounced in Vita 5. Furthermore, leaf area was also reduced in this cultivar. Chlorophyll content and chlorophyll fluorescence parameters were not affected by salt stress, but the specific activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) decreased in Vita 3 and increased in Vita 5. The use of immunological techniques also revealed that the Rubisco content from Vita 3 decreased while that of Vita 5 increased. The discussion of these results is aimed at reaching a better understanding of the differences between these cultivars in relation to salt stress.

Effect of Branched Broomrape (Orobanche ramosa) Infection on the Growth and Photosynthesis of Tomato

Weed Science ◽  
2008 ◽  
Vol 56 (4) ◽  
pp. 574-581 ◽  
Author(s):  
Giovanni Mauromicale ◽  
Antonino Lo Monaco ◽  
Angela M. G. Longo
Keyword(s):  
Chlorophyll Fluorescence ◽  
Chlorophyll Content ◽  
Photosynthetic Rate ◽  
Carbon Assimilation ◽  
Parasitic Plants ◽  
Chlorophyll Fluorescence Parameters ◽  
Leaf Chlorophyll Content ◽  
Fluorescence Parameters ◽  
Growing Seasons ◽  
Leaf Chlorophyll

The influence of the holoparasite branched broomrape on the vegetative growth, leaf chlorophyll content, photosynthetic rate, and chlorophyll fluorescence of tomato was studied over two growing seasons on plants grown in a commercial greenhouse. The presence of the parasite strongly reduced the aerial biomass by acting as a competing sink for assimilate, but more importantly, by compromising the efficiency of carbon assimilation via a reduction in leaf chlorophyll content and photosynthetic rate. The chlorophyll fluorescence parameters F0, Fm, Fv, and Fv/Fm were all altered in parasitized plants, indicating that branched broomrape–infected plants are more susceptible to photoinhibition. The degree of damage to the host was not dependent on either the number or the biomass of parasitic plants per host plant. We suggest that the ability to maintain a high photosynthetic rate, leaf chlorophyll content, or both and the ability to minimize photoinhibition can be developed as indirect assays for improved tolerance to branched broomrape.

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