scholarly journals Shoot Characterization of Isoprene and Ocimene-Emitting Transgenic Arabidopsis Plants under Contrasting Environmental Conditions

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 477 ◽  
Author(s):  
Michele Faralli ◽  
Mingai Li ◽  
Claudio Varotto
Keyword(s):  
Water Stress ◽  
Stress Tolerance ◽  
Stress Responses ◽  
Water Conservation ◽  
Transgenic Arabidopsis ◽  
Stomatal Closure ◽  
Crop Improvement ◽  
Dry Weight ◽  
Co2 Assimilation ◽  
Biotic And Abiotic Stress

Isoprenoids are among the most abundant biogenic volatile compounds (VOCs) emitted by plants, and mediate both biotic and abiotic stress responses. Here, we provide for the first time a comparative analysis of transgenic Arabidopsis lines constitutively emitting isoprene and ocimene. Transgenic lines and Columbia-0 (Col-0) Arabidopsis were characterized under optimal, water stress, and heat stress conditions. Under optimal conditions, the projected leaf area (PLA), relative growth rate, and final dry weight were generally higher in transgenics than Col-0. These traits were associated to a larger photosynthetic capacity and CO2 assimilation rate at saturating light. Isoprene and ocimene emitters displayed a moderately higher stress tolerance than Col-0, showing higher PLA and gas-exchange traits throughout the experiments. Contrasting behaviors were recorded for the two overexpressors under water stress, with isoprene emitters showing earlier stomatal closure (conservative behavior) than ocimene emitters (non-conservative behavior), which might suggest different induced strategies for water conservation and stress adaptation. Our work indicates that (i) isoprene and ocimene emitters resulted in enhanced PLA and biomass under optimal and control conditions and that (ii) a moderate stress tolerance is induced when isoprene and ocimene are constitutively emitted in Arabidopsis, thus providing evidence of their role as a potential preferable trait for crop improvement.

scholarly journals Functional genomics to study stress responses in crop legumes: progress and prospects

2013 ◽  
Vol 40 (12) ◽  
pp. 1221 ◽  
Author(s):  
Himabindu Kudapa ◽  
Abirami Ramalingam ◽  
Swapna Nayakoti ◽  
Xiaoping Chen ◽  
Wei-Jian Zhuang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Functional Genomics ◽  
Stress Tolerance ◽  
Stress Responses ◽  
Molecular Mechanisms ◽  
Crop Improvement ◽  
Biotic And Abiotic Stress ◽  
Biotic Stresses ◽  
Adverse Conditions ◽  
Recent Advances

Legumes are important food crops worldwide, contributing to more than 33% of human dietary protein. The production of crop legumes is frequently impacted by abiotic and biotic stresses. It is therefore important to identify genes conferring resistance to biotic stresses and tolerance to abiotic stresses that can be used to both understand molecular mechanisms of plant response to the environment and to accelerate crop improvement. Recent advances in genomics offer a range of approaches such as the sequencing of genomes and transcriptomes, gene expression microarray as well as RNA-seq based gene expression profiling, and map-based cloning for the identification and isolation of biotic and abiotic stress-responsive genes in several crop legumes. These candidate stress associated genes should provide insights into the molecular mechanisms of stress tolerance and ultimately help to develop legume varieties with improved stress tolerance and productivity under adverse conditions. This review provides an overview on recent advances in the functional genomics of crop legumes that includes the discovery as well as validation of candidate genes.

Physiological and morphological responses to water stress in Aegilops biuncialis and Triticum aestivum genotypes with differing tolerance to drought

2004 ◽  
Vol 31 (12) ◽  
pp. 1149 ◽  
Author(s):  
István Molnár ◽  
László Gáspár ◽  
Éva Sárvári ◽  
Sándor Dulai ◽  
Borbála Hoffmann ◽  
...  
Keyword(s):  
Growth Rate ◽  
Triticum Aestivum ◽  
Water Stress ◽  
Drought Tolerance ◽  
Osmotic Stress ◽  
Biomass Production ◽  
Water Loss ◽  
Stomatal Closure ◽  
Co2 Assimilation ◽  
Wheat Genotypes

The physiological and morphological responses to water stress induced by polyethylene glycol (PEG) or by withholding water were investigated in Aegilops biuncialis Vis. genotypes differing in the annual rainfall of their habitat (1050, 550 and 225 mm year–1) and in Triticum aestivum L. wheat genotypes differing in drought tolerance. A decrease in the osmotic pressure of the nutrient solution from –0.027 to –1.8 MPa resulted in significant water loss, a low degree of stomatal closure and a decrease in the intercellular CO2 concentration (Ci) in Aegilops genotypes originating from dry habitats, while in wheat genotypes high osmotic stress increased stomatal closure, resulting in a low level of water loss and high Ci. Nevertheless, under saturating light at normal atmospheric CO2 levels, the rate of CO2 assimilation was higher for the Aegilops accessions, under high osmotic stress, than for the wheat genotypes. Moreover, in the wheat genotypes CO2 assimilation exhibited less or no O2 sensitivity. These physiological responses were manifested in changes in the growth rate and biomass production, since Aegilops (Ae550, Ae225) genotypes retained a higher growth rate (especially in the roots), biomass production and yield formation after drought stress than wheat. These results indicate that Aegilops genotypes, originating from a dry habitat have better drought tolerance than wheat, making them good candidates for improving the drought tolerance of wheat through intergeneric crossing.

scholarly journals Genomic Analysis of Serratia plymuthica MBSA-MJ1: A Plant Growth Promoting Rhizobacteria That Improves Water Stress Tolerance in Greenhouse Ornamentals

2021 ◽  
Vol 12 ◽  
Author(s):  
Nathan P. Nordstedt ◽  
Michelle L. Jones
Keyword(s):  
Water Stress ◽  
Plant Growth ◽  
Stress Tolerance ◽  
Stress Responses ◽  
Plant Growth Promoting Rhizobacteria ◽  
Serratia Plymuthica ◽  
Water Stress Tolerance ◽  
Plant Growth Promoting ◽  
Growth Promoting

Water stress decreases the health and quality of horticulture crops by inhibiting photosynthesis, transpiration, and nutrient uptake. Application of plant growth promoting rhizobacteria (PGPR) can increase the growth, stress tolerance, and overall quality of field and greenhouse grown crops subjected to water stress. Here, we evaluated Serratia plymuthica MBSA-MJ1 for its ability to increase plant growth and quality of Petunia × hybrida (petunia), Impatiens walleriana (impatiens), and Viola × wittrockiana (pansy) plants recovering from severe water stress. Plants were treated weekly with inoculum of MBSA-MJ1, and plant growth and quality were evaluated 2 weeks after recovery from water stress. Application of S. plymuthica MBSA-MJ1 increased the visual quality and shoot biomass of petunia and impatiens and increased the flower number of petunia after recovery from water stress. In addition, in vitro characterizations showed that MBSA-MJ1 is a motile bacterium with moderate levels of antibiotic resistance that can withstand osmotic stress. Further, comprehensive genomic analyses identified genes putatively involved in bacterial osmotic and oxidative stress responses and the synthesis of osmoprotectants and vitamins that could potentially be involved in increasing plant water stress tolerance. This work provides a better understanding of potential mechanisms involved in beneficial plant-microbe interactions under abiotic stress using a novel S. plymuthica strain as a model.

Effect of water stress on growth, water use efficiency and gas exchange as related to osmotic adjustment of two halophytes Atriplex spp.

2013 ◽  
Vol 40 (5) ◽  
pp. 466 ◽  
Author(s):  
Oumelkheir Belkheiri ◽  
Maurizio Mulas
Keyword(s):  
Water Stress ◽  
Water Use Efficiency ◽  
Water Use ◽  
Osmotic Adjustment ◽  
Late Summer ◽  
Field Capacity ◽  
Dry Weight ◽  
Co2 Assimilation ◽  
Effect Of Water ◽  
Use Efficiency

Atriplex halimus L. is known in the Mediterranean basin and along the coastal areas of Sardinia for its adaptability to salinity, although less information is available on the resistance of this species to water stress in absence of salinity. The effect of water stress on growth and water utilisation was investigated in two Atriplex species: A. halimus originating of south Sardinian island and the exotic species Atriplex nummularia Lindl., originating in Australia and widely used in land restoration of arid areas. Water stress was applied to young plants growing in 20 L pots with a sufficient water reserve to store a potentially sufficient water reserve to maintain substrate near to field capacity (30%) between irrigations. Watering was at 70% (control) or 40% (stress) of field capacity. In order to simulate the grazing by livestock, four plant biomass cuttings were conducted at times T0, T1, T2 and T3, corresponding to one cutting at the end of well watered phase (T0) before water stress induction, two cuttings after cycles of 5 weeks each during full summer (T1) and late summer (T2) and one cutting during autumn (T3). All plants remained alive until the end of treatment although growth was strongly reduced. Leaf dry weight (DW) and water use efficiency (WUE) were determined for all cuttings; relative water content (RWC), turgid weight : dry weight ratio (TW : DW), water potential (Ψw), osmotic potential (Ψs), CO2 assimilation, osmotic adjustment (OA), abscisic acid (ABA) and sugar accumulation were determined for the late summer cutting at T2. Water stress induced a decrease in DW, RWC, Ψw, Ψs, TW : DW and CO2 assimilation for both species, but an increase in WUE expressed in terms of dry matter production and a high accumulation of ABA and total sugars mainly for A. halimus. This suggests a more developed adaptive mechanism in this selection. Indeed, the clone was selected from the southern part of the island, where natural populations of saltbush are more exposed to abiotic stresses, mainly the water stress generated not by salinity. A. nummularia showed a greater OA and a positive net solute accumulation as than A. halimus, suggesting that water stress resistance in A. halimus is linked to a higher WUE rather than a greater osmotic adjustment.

scholarly journals A tubby-like protein CsTLP8 acts in the ABA signaling pathway and negatively regulates osmotic stresses tolerance during seed germination

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Shuangtao Li ◽  
Zhirong Wang ◽  
Fei Wang ◽  
Hongmei Lv ◽  
Meng Cao ◽  
...  
Keyword(s):  
Seed Germination ◽  
Osmotic Stress ◽  
Stress Responses ◽  
Transcriptional Activation ◽  
Stomatal Closure ◽  
Negative Regulator ◽  
Biotic And Abiotic Stress ◽  
Growth Activity ◽  
A Subunit ◽  
Antioxidant Enzymes Activities

Abstract Background TLPs (Tubby-like proteins) are widespread in eukaryotes and highly conserved in plants and animals. TLP is involved in many biological processes, such as growth, development, biotic and abiotic stress responses, while the underlying molecular mechanism remains largely unknown. In this paper we characterized the biological function of cucumber (Cucumis sativus L.) Tubby-like protein 8 (CsTLP8) in Arabidopsis. Results In cucumber, the expression of the tubby-like protein CsTLP8 was induced by NaCl treatment, but reduced by PEG (Polyethylene Glycol) and ABA (Abscisic Acid) treatment. Subcellular localization and transcriptional activation activity analysis revealed that CsTLP8 possessed two characteristics of classical transcription factors: nuclear localization and trans-activation activity. Yeast two-hybrid assay revealed interactions of CsTLP8 with CsSKP1a and CsSKP1c, suggesting that CsTLP8 might function as a subunit of E3 ubiquitin ligase. The growth activity of yeast with ectopically expressed CsTLP8 was lower than the control under NaCl and mannitol treatments. Under osmotic and salt stresses, overexpression of CsTLP8 inhibited seed germination and the growth of Arabidopsis seedlings, increased the content of MDA (Malondialdehyde), and decreased the activities of SOD (Superoxide Dismutase), POD (Peroxidase) and CAT (Catalase) in Arabidopsis seedlings. Overexpression of CsTLP8 also increased the sensitivity to ABA during seed germination and ABA-mediated stomatal closure. Conclusion Under osmotic stress, CsTLP8 might inhibit seed germination and seedling growth by affecting antioxidant enzymes activities. CsTLP8 acts as a negative regulator in osmotic stress and its effects may be related to ABA.

scholarly journals Biostimulation of Maize (Zea mays) and Irrigation Management Improved Crop Growth and Water Use under Controlled Environment

Agronomy ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 559 ◽  
Author(s):  
Lin ◽  
Lin ◽  
Wu ◽  
Chang
Keyword(s):  
Zea Mays ◽  
Water Stress ◽  
Stress Tolerance ◽  
Water Use ◽  
Crop Production ◽  
Irrigation Management ◽  
Field Capacity ◽  
Dry Weight ◽  
Water Stress Tolerance ◽  
Total Dry Weight

Water deficits during the growing season are a major factor limiting crop production. Therefore, reducing water use during crop production by the application of regulated deficit irrigation (RDI) is crucially important in water resources. There are few reports on the biostimulants used for growth and water use efficiency (WUE) in maize (Zea mays Linn.) under RDI. Therefore, the influence of betaine and chitin treatments, alone and in combination, on maize cultivar ‘White Pearl’ was assessed by observing changes in the physiology and morphology of plants exposed to RDI. Plants were grown in plastic pots in greenhouses and maintained under full irrigation (FI) for 1 week until imposing RDI and biostimulants. Plants were then subjected to FI (no water deficiency treatment, field capacity >70%) and RDI (field capacity <50%) conditions until the end of each experiment. Plant agronomic performance, photosynthesis parameters, and WUE values were recorded weekly for 8 weeks and three individual experiments were carried out to assess the efficacy of biostimulants and irrigation treatments. Betaine (0, 50, and 100 mM/plant) was foliage-treated every 2 weeks during Experiment 1, but chitin (0, 2, and 4 g/kg) was applied to the soil at the beginning of Experiment 2. The optimal concentration of each chemical alone or in combination was then applied to the plants as Experiment 3. A factorial experiment design of two factors with different levels under a completely randomized arrangement was used in this investigation. Betaine (50 mM) or chitin (2 g/kg) treatments alone significantly elevated total fresh weight (63.03 or 124.07 g/plant), dry weight (18.00 or 22.34 g/plant), and cob weight (3.15 or 6.04 g/plant) and boosted the water-stress tolerance of the maize under RDI compared to controls. However, a combination treatment of 50 mM betaine and 2 g/kg chitin did not increase plant height, fresh shoot and root weights, dry cob weight, and total dry weight under RDI compared to controls. Soil-plant analysis development (SPAD) values (>30) were effective in detecting plant growth performance and WUE values under RDI. These findings may have greater significance for farming in dry lands and offer information for further physiological studies on maize WUE and water stress tolerance

scholarly journals Hyperspectral and Thermal Sensing of Stomatal Conductance, Transpiration, and Photosynthesis for Soybean and Maize under Drought

2020 ◽  
Vol 12 (19) ◽  
pp. 3182
Author(s):  
Verónica Sobejano-Paz ◽  
Teis Nørgaard Mikkelsen ◽  
Andreas Baum ◽  
Xingguo Mo ◽  
Suxia Liu ◽  
...  
Keyword(s):  
Remote Sensing ◽  
Water Stress ◽  
Stomatal Conductance ◽  
Stress Responses ◽  
Stomatal Closure ◽  
Hyperspectral Data ◽  
Morphological Properties ◽  
Leaf Physiology ◽  
Spectral Bands ◽  
Sensing Applications

During water stress, crops undertake adjustments in functional, structural, and biochemical traits. Hyperspectral data and machine learning techniques (PLS-R) can be used to assess water stress responses in plant physiology. In this study, we investigated the potential of hyperspectral optical (VNIR) measurements supplemented with thermal remote sensing and canopy height (hc) to detect changes in leaf physiology of soybean (C3) and maize (C4) plants under three levels of soil moisture in controlled environmental conditions. We measured canopy evapotranspiration (ET), leaf transpiration (Tr), leaf stomatal conductance (gs), leaf photosynthesis (A), leaf chlorophyll content and morphological properties (hc and LAI), as well as vegetation cover reflectance and radiometric temperature (TL,Rad). Our results showed that water stress caused significant ET decreases in both crops. This reduction was linked to tighter stomatal control for soybean plants, whereas LAI changes were the primary control on maize ET. Spectral vegetation indices (VIs) and TL,Rad were able to track these different responses to drought, but only after controlling for confounding changes in phenology. PLS-R modeling of gs, Tr, and A using hyperspectral data was more accurate when pooling data from both crops together rather than individually. Nonetheless, separated PLS-R crop models are useful to identify the most relevant variables in each crop such as TL,Rad for soybean and hc for maize under our experimental conditions. Interestingly, the most important spectral bands sensitive to drought, derived from PLS-R analysis, were not exactly centered at the same wavelengths of the studied VIs sensitive to drought, highlighting the benefit of having contiguous narrow spectral bands to predict leaf physiology and suggesting different wavelength combinations based on crop type. Our results are only a first but a promising step towards larger scale remote sensing applications (e.g., airborne and satellite). PLS-R estimates of leaf physiology could help to parameterize canopy level GPP or ET models and to identify different photosynthetic paths or the degree of stomatal closure in response to drought.

Overexpression of DnWRKY11 enhanced salt and drought stress tolerance of transgenic tobacco

Biologia ◽  
2014 ◽  
Vol 69 (8) ◽  
Author(s):  
Xiang-Bin Xu ◽  
Yuan-Yuan Pan ◽  
Chun-Ling Wang ◽  
Qi-Cai Ying ◽  
Hong-Miao Song ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Stress Responses ◽  
Environmental Changes ◽  
Germination Rate ◽  
Defense Responses ◽  
Biotic And Abiotic Stress ◽  
Field Environment

AbstractDendrobium seedlings showed low survival rate when they were transferred from in vitro conditions to greenhouse or field environment. One of the major reasons is their low tolerance to environmental changes. WRKY transcription factors are one of the largest families of transcriptional regulators in plants. They are involved in various biotic and abiotic stress responses. One DnWRKY11 gene was isolated from Dendrobium nobile. To explore the function of DnWRKY11 in Dendrobium defense responses to abiotic stress, it was overexpressed in tobacco. Under salt and drought stresses, the DnWRKY11 transgenic tobacco showed higher germination rate, longer root length, higher fresh weight, higher activities of catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), and lower content of malonidialdehyde (MDA) than the wild type. These results proved the important roles of DnWRKY11 in plant response to drought and salt stresses, and provided a potential gene for improving environmental stress tolerance of Dendrobium seedlings.

scholarly journals Application of Genome Editing in Tomato Breeding: Mechanisms, Advances, and Prospects

2021 ◽  
Vol 22 (2) ◽  
pp. 682
Author(s):  
Hymavathi Salava ◽  
Sravankumar Thula ◽  
Vijee Mohan ◽  
Rahul Kumar ◽  
Fatemeh Maghuly
Keyword(s):  
Abiotic Stress ◽  
Stress Tolerance ◽  
Genome Editing ◽  
Stress Responses ◽  
Abiotic Stresses ◽  
Nutritional Quality ◽  
Insertional Mutagenesis ◽  
Crop Improvement ◽  
Functional Characterization ◽  
Crop Plants

Plants regularly face the changing climatic conditions that cause biotic and abiotic stress responses. The abiotic stresses are the primary constraints affecting crop yield and nutritional quality in many crop plants. The advances in genome sequencing and high-throughput approaches have enabled the researchers to use genome editing tools for the functional characterization of many genes useful for crop improvement. The present review focuses on the genome editing tools for improving many traits such as disease resistance, abiotic stress tolerance, yield, quality, and nutritional aspects of tomato. Many candidate genes conferring tolerance to abiotic stresses such as heat, cold, drought, and salinity stress have been successfully manipulated by gene modification and editing techniques such as RNA interference, insertional mutagenesis, and clustered regularly interspaced short palindromic repeat (CRISPR/Cas9). In this regard, the genome editing tools such as CRISPR/Cas9, which is a fast and efficient technology that can be exploited to explore the genetic resources for the improvement of tomato and other crop plants in terms of stress tolerance and nutritional quality. The review presents examples of gene editing responsible for conferring both biotic and abiotic stresses in tomato simultaneously. The literature on using this powerful technology to improve fruit quality, yield, and nutritional aspects in tomato is highlighted. Finally, the prospects and challenges of genome editing, public and political acceptance in tomato are discussed.

scholarly journals Interspecific Differences in Physiological and Biochemical Traits Drive the Water Stress Tolerance in Young Morus alba L. and Conocarpus erectus L. Saplings

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1615
Author(s):  
Zikria Zafar ◽  
Fahad Rasheed ◽  
Ahsan Ul Haq ◽  
Faridah Hanum Ibrahim ◽  
Shazia Afzal ◽  
...  
Keyword(s):  
Water Stress ◽  
Stress Tolerance ◽  
Soil Water ◽  
Water Deficit ◽  
High Stress ◽  
Field Capacity ◽  
Dry Weight ◽  
Soil Water Deficit ◽  
Water Stress Tolerance ◽  
Intrinsic Water Use Efficiency

Mitigating climate change requires the identification of tree species that can tolerate water stress with fewer negative impacts on plant productivity. Therefore, the study aimed to evaluate the water stress tolerance of young saplings of C. erectus and M. alba under three soil water deficit treatments (control, CK, 90% field capacity, FC, medium stress MS, 60% FC and high stress, HS, 30% FC) under controlled conditions. Results showed that leaf and stem dry weight decreased significantly in both species under MS and HS. However, root dry weight and root/shoot ratio increased, and total dry weight remained similar to CK under MS in C. erectus saplings. Stomatal conductance, CO2 assimilation rate decreased, and intrinsic water use efficiency increased significantly in both species under MS and HS treatments. The concentration of hydrogen peroxide, superoxide radical, malondialdehyde and electrolyte leakage increased in both the species under soil water deficit but was highest in M. alba. The concentration of antioxidative enzymes like superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase also increased in both species under MS and HS but was highest in C. erectus. Therefore, results suggest that C. erectus saplings depicted a better tolerance to MS due to an effective antioxidative enzyme system.

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