scholarly journals AgNO3 Sterilizes Grains of Barley (Hordeum vulgare) without Inhibiting Germination—A Necessary Tool for Plant–Microbiome Research

Plants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 372
Author(s):  
Victoria Munkager ◽  
Mette Vestergård ◽  
Anders Priemé ◽  
Andreas Altenburger ◽  
Eva de Visser ◽  
...  
Keyword(s):  
Plant Biomass ◽  
Growth Promotion ◽  
Salt Water ◽  
Germination Percentage ◽  
Experimental Plant ◽  
Standardized Protocol ◽  
Microbiome Research ◽  
Barley Grains ◽  
Microscopic Inspection ◽  
Plant Microbiome

To understand and manipulate the interactions between plants and microorganisms, sterile seeds are a necessity. The seed microbiome (inside and surface microorganisms) is unknown for most plant species and seed-borne microorganisms can persist and transfer to the seedling and rhizosphere, thereby obscuring the effects that purposely introduced microorganisms have on plants. This necessitates that these unidentified, seed-borne microorganisms are removed before seeds are used for studies on plant–microbiome interactions. Unfortunately, there is no single, standardized protocol for seed sterilization, hampering progress in experimental plant growth promotion and our study shows that commonly applied sterilization protocols for barley grains using H2O2, NaClO, and AgNO3 yielded insufficient sterilization. We therefore developed a sterilization protocol with AgNO3 by testing several concentrations of AgNO3 and added two additional steps: Soaking the grains in water before the sterilization and rinsing with salt water (1% (w/w) NaCl) after the sterilization. The most efficient sterilization protocol was to soak the grains, sterilize with 10% (w/w) AgNO3, and to rinse with salt water. By following those three steps, 97% of the grains had no culturable, viable microorganism after 21 days based on microscopic inspection. The protocol left small quantities of AgNO3 residue on the grain, maintained germination percentage similar to unsterilized grains, and plant biomass was unaltered. Hence, our protocol using AgNO3 can be used successfully for experiments on plant–microbiome interactions.

scholarly journals Establishing Causality: Opportunities of Synthetic Communities for Plant Microbiome Research

2017 ◽  
Vol 22 (2) ◽  
pp. 142-155 ◽  
Author(s):  
Julia A. Vorholt ◽  
Christine Vogel ◽  
Charlotte I. Carlström ◽  
Daniel B. Müller
Keyword(s):  
Microbiome Research ◽  
Plant Microbiome

Growth Promotion Utility of the Plant Microbiome

2021 ◽  
pp. 307-319
Author(s):  
S. Kalaiselvi ◽  
A. Panneerselvam
Keyword(s):  
Growth Promotion ◽  
Plant Microbiome

scholarly journals Mitigation of Nickel Toxicity and Growth Promotion in Sesame through the Application of a Bacterial Endophyte and Zeolite in Nickel Contaminated Soil

2020 ◽  
Vol 17 (23) ◽  
pp. 8859 ◽  
Author(s):  
Muhammad Naveed ◽  
Syeda Sosan Bukhari ◽  
Adnan Mustafa ◽  
Allah Ditta ◽  
Saud Alamri ◽  
...  
Keyword(s):  
Plant Growth ◽  
Contaminated Soil ◽  
Root Zone ◽  
Plant Biomass ◽  
Growth Promotion ◽  
Research Work ◽  
Control Treatment ◽  
Soil Conditions ◽  
Combined Application ◽  
Bioavailable Fraction

Nickel (Ni) bioavailable fraction in the soil is of utmost importance because of its involvement in plant growth and environmental feedbacks. High concentrations of Ni in the soil environment, especially in the root zone, may retard plant growth that ultimately results in reduced plant biomass and yield. However, endophytic microorganisms have great potential to reduce the toxicity of Ni, especially when applied together with zeolite. The present research work was conducted to evaluate the potential effects of an endophytic bacterium Caulobacter sp. MN13 in combination with zeolite on the physiology, growth, quality, and yield of sesame plant under normal and Ni stressed soil conditions through possible reduction of Ni uptake. Surface sterilized sesame seeds were sown in pots filled with artificially Ni contaminated soil amended with zeolite. Results revealed that plant agronomic attributes such as shoot root dry weight, total number of pods, and 1000-grains weight were increased by 41, 45, 54, and 65%, respectively, over control treatment, with combined application of bacteria and zeolite in Ni contaminated soil. In comparison to control, the gaseous exchange parameters (CO2 assimilation rate, transpiration rate, stomatal- sub-stomatal conductance, chlorophyll content, and vapor pressure) were significantly enhanced by co-application of bacteria and zeolite ranging from 20 to 49% under Ni stress. Moreover, the combined utilization of bacteria and zeolite considerably improved water relations of sesame plant, in terms of relative water content (RWC) and relative membrane permeability (RMP) along with improvement in biochemical components (protein, ash, crude fiber, fat), and micronutrients in normal as well as in Ni contaminated soil. Moreover, the same treatment modulated the Ni-stress in plants through improvement in antioxidant enzymes (AEs) activities along with improved Ni concentration in the soil and different plant tissues. Correlation and principal component analysis (PCA) further revealed that combined application of metal-tolerant bacterium Caulobacter sp. MN13 and zeolite is the most influential strategy in alleviating Ni-induced stress and subsequent improvement in growth, yield, and physio-biochemical attributes of sesame plant.

Pseudomonads contribute to regulation ofPratylenchus penetrans(Nematoda) populations on apple

2018 ◽  
Vol 64 (11) ◽  
pp. 775-785 ◽  
Author(s):  
Tristan T. Watson ◽  
Tom A. Forge ◽  
Louise M. Nelson
Keyword(s):  
Pseudomonas Fluorescens ◽  
Protease Activity ◽  
Fungal Pathogens ◽  
Plant Biomass ◽  
Growth Promotion ◽  
In Vitro Growth ◽  
Pratylenchus Penetrans ◽  
Pseudomonas Spp ◽  
Orchard Soil

Inoculation with antagonistic soil microorganisms has shown potential to suppress replant disease of apple in orchard soils. Pseudomonas spp. may have the potential to reduce Pratylenchus penetrans populations on apple. Pseudomonas spp. were isolated from the rhizosphere of sweet cherry and screened for antagonistic characteristics. Two highly antagonistic Pseudomonas isolates, P10-32 and P10-42, were evaluated for growth promotion of apple seedlings, suppression of P. penetrans populations, and root colonization in soil from three orchards. During the isolate screening, Pseudomonas fluorescens P10-32 reduced in vitro growth of fungal pathogens, had protease activity, had capacity to produce pyrrolnitrin, suppressed P. penetrans populations, and increased plant biomass. Pseudomonas fluorescens P10-42 reduced in vitro growth of fungal pathogens, had protease activity, suppressed P. penetrans populations, and increased plant biomass. In potted orchard soil, inoculating apple with P. fluorescens P10-32 suppressed P. penetrans populations in one of the three soils examined. Inoculation with P. fluorescens P10-42 improved plant growth in two of the soils and suppressed P. penetrans abundance in one soil. In one of the soils, P. fluorescens P10-42 was detected on the roots 56 days postinoculation. Overall, we conclude that Pseudomonas spp. play a role in suppressing P. penetrans on apple in orchard soil.

scholarly journals Salicylic acid pre-treatment induced physiological and biochemical changes in Solanum lycopersicum L. under salinity stress

2021 ◽  
Vol 13 (2) ◽  
pp. 10917
Author(s):  
Venu SREELAKSHMY ◽  
Gunasekar ANBARASI ◽  
Benaltraja VISHNUPRIYA
Keyword(s):  
Salicylic Acid ◽  
Salt Stress ◽  
Plant Growth ◽  
Solanum Lycopersicum ◽  
Crop Production ◽  
Plant Biomass ◽  
Salt Water ◽  
Radical Scavenging ◽  
Agricultural Crop ◽  
Pre Treatment

Agricultural crop production around the world is adversely affected by excess salt accumulation in the soil. Plants initiate broad range of signal transduction pathways to respond any stress. Salicylic acid (SA) is an endogenous plant growth regulator that acts as a signal molecule to modulate plant response by reducing the effects of abiotic stress on plants. The main objective of this study is to examine whether exogenous salicylic acid pre-treatment may reduce the adverse effects of salt stress and enhance salt tolerance in Solanum lycopersicum. For this experiment, two weeks old seedlings were subjected to salt stress by adding salt water (100 mM NaCl) for three days with or without salicylic acid pre-treatment. After salt stress exposure plant leaves were harvested and the various measures were recorded. Results of this study exhibited that salicylic acid pre-treatment mitigates various advers effects of salt stress on plant growth by stimulating plant biomass, water relations, protein content, chlorophyll pigment, and inorganic osmolytes accumulation. Simultaneously, an increase in activity of antioxidant enzymes of SOD, CAT and POX were also triggered.  This current study suggested that pre-treating of Solanum lycopersicum with salicylic acid attenuates the depressive effect of salinity by accelerating the osmolyte accumulation and triggering activity of free radical scavenging enzymes.

scholarly journals Phytostimulation and growth promotion activities of Trichoderma spp. on groundnut (Arachis hypogaea L.) crop

2021 ◽  
Vol 13 (4) ◽  
pp. 1172-1179
Author(s):  
M. Ayyandurai ◽  
R. Akila ◽  
K. Manonmani ◽  
M. Theradimani ◽  
S. Vellaikumar
Keyword(s):  
Arachis Hypogaea ◽  
Growth Promotion ◽  
Pcr Amplification ◽  
Its Region ◽  
Germination Percentage ◽  
High Yield ◽  
Trichoderma Spp ◽  
Soil Borne Pathogens ◽  
Trichoderma Sp ◽  
Arachis Hypogaea L

Groundnut (Arachis hypogaea L.) suffers from many soil borne pathogens that deteriorate the quality of the seeds and are responsible for high yield loss. Practically Trichoderma sp. is used for seed treatment, it minimizes the seed and soil borne pathogens and supports plant growth promotion activities. In the present study, five different isolates of Trichoderma spp. were isolated from groundnut (A. hypogaea ) rhizosphere soil. All the five isolates were confirmed by morphological methods and using molecular tools through Polymerase Chain Reaction (PCR) amplification of Internal Transcribed Spacer (ITS) region of Trichoderma sp. and DNA gets amplified in 650 bp to 700 bp. Trichoderma spp. were molecularly identified as T(SP)-20 (Trichoderma longibrachiatum), T(AR)-10 (T. asperellum), T(VT)-3 (T. hamatum), T(BI)-16 (T. longibrachiatum), T(TK)-23 (T. citrinoviride). Phytostimulation activities of all the six isolates viz., phosphate solubilization, Ammonia production, IAA production, and Siderophore production, were evaluated. Among the six isolates, T(SP)-20, T(AR)-10, and TNAU-TA showed higher phytostimulation activities. The growth promotion of Trichoderma spp. on groundnut was assessed through the roll towel method. The isolate T(SP)-20 (T. longibrachiatum) produced the highest germination percentage of 93.33 and vigor index of 2246.2. This work developed a new isolate of T. longibrachiatum (T(SP)-20) which is a native isolate having significant  phytostimulation and growth promotion activities and it could be exploited for other soil borne disease managing successfully.

scholarly journals Effect of Light Emitting Diode (LED) Spectrum at Seedlings Production for Optimal Growth on Different Type of Lettuce in MARDI Plant Factory

2021 ◽  
Author(s):  
Mohamed Hafeifi Basir ◽  
Intan Nadhirah Masri
Keyword(s):  
Leaf Area ◽  
Plant Biomass ◽  
Light Emitting Diode ◽  
Germination Percentage ◽  
Seedling Production ◽  
Full Spectrum ◽  
Spad Value ◽  
Fresh Vegetables ◽  
Plant Factory ◽  
Production Area

Seedling production is a crucial part of the production of fresh vegetables in a plant factory. Light is one of the necessities for plants to produce a healthy seedling before being transplanted to the production area. Different light formulations resulted in different growth performances of the plant. Hence, this study was conducted to aim for suitable light formulation on various types of lettuce in the MARDI Plant Factory. The study was conducted in two stages: 1) seedling production and 2) production area. Treatments were evaluated at the seedlings' production stage using the split-plot experimental design with four replications. LED light treatments (LT) was the main factor with the various ratio of spectrum colour of Red (R), Blue (B), Green (G) and full spectrum. (LT 1; 5R:1B, LT 2; 1R:1B, LT 3; 1R: 2B, LT 4; 2R:1B, LT 5; 4R:1B:1G and LT 6; Full spectrum as control). The sub-factor was lettuce variety (V1; Butterhead, V2; Green Coral, V3; Red Coral and V4; Mini Cos). Variables measured at seedlings production were seed germination. Growth biomass and SPAD value were evaluated in the production area. At seedlings production, the full spectrum lighting shows significant seeds germination percentage compared to other LED lighting, and V1 performed well on germination percentage and time compare to other varieties. The interaction between light treatments and lettuce was observed on the leaf numbers, shoot fresh weight, leaf area, and the shoot-root ratio at the production area. LT 1 and LT 5 on butterhead and green coral significantly affected the number of leaves and leaf area, which were relatively influenced by light quality and ambient temperature. The yield on green coral lettuce grown under LT 1, LT 2, and LT 5 was significantly higher than others. However, plant biomass and SPAD value for all treatments were not significantly different. The allometry of plant was expressed on a shoot-root ratio with LT 2 on green coral shows a significantly higher shoot-root ratio than other treatments. The study's findings showed that light treatment with Red and Blue LED ratio of 5:1, 1:1, and Red, Blue, and Green LED ratio of 4:1:1 light arrangement on the seedling's productions provided optimal growing conditions in the production area butterhead and green coral lettuce in MPF cultivation.

scholarly journals Effect of waste Al-phosphate on soil and plant  

2013 ◽  
Vol 59 (No. 3) ◽  
pp. 130-135 ◽  
Author(s):  
Amaizah NR ◽  
D. Cakmak ◽  
E. Saljnikov ◽  
G. Roglic ◽  
N. Kokovic ◽  
...  
Keyword(s):  
Sequential Extraction ◽  
Crop Production ◽  
Plant Biomass ◽  
Dry Weight ◽  
Phosphate Fertilizer ◽  
Aluminum Phosphate ◽  
Soil Types ◽  
Available Phosphorus ◽  
Experimental Plant ◽  
Alternative Sources

Irreplaceability of phosphorus as a necessary macroelement in crop production is due to limited resources and costly processing of ores and immobilization in soil, which force for seeking an alternative sources or the use of waste materials. In this paper, the waste aluminum phosphate from pharmaceutical factory used as phosphate fertilizer and its effects were compared with other phosphorus fertilizers (superphosphate and rock phosphate). Except the analysis of available phosphorus (AL-method) the sequential extraction of phosphorus (modified Chang and Jackson) and sequential extraction of aluminum (modified Tessier) were performed. The experimental plant was mustard (Sinapis alba). The pot experiment was carried out on two soil types: Stagnosol and Vertisol. Application of phosphorus with aluminum phosphate had the same effect as the application of other phosphatic fertilizers in both soil types. In Stagnosol Al-phosphate directly influenced the increase in plant fresh weight by 39% and dry weight by 43% compared to the control, and also decreased the content of mobile Al for 40% and Pb for 47% in plant biomass. Based on these results, the use of waste aluminum phosphate has a potential to be used as a phosphorus fertilizer under given conditions.

scholarly journals Holo-omics for deciphering plant-microbiome interactions

Microbiome ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Ling Xu ◽  
Grady Pierroz ◽  
Heidi M.-L. Wipf ◽  
Cheng Gao ◽  
John W. Taylor ◽  
...  
Keyword(s):  
Heterogeneous Data ◽  
Research Field ◽  
System Level ◽  
Molecular Networks ◽  
Functional Relationships ◽  
Powerful Approach ◽  
Host Health ◽  
Microbiome Research ◽  
Analytical Frameworks ◽  
Plant Microbiome

AbstractHost-microbiome interactions are recognized for their importance to host health. An improved understanding of the molecular underpinnings of host-microbiome relationships will advance our capacity to accurately predict host fitness and manipulate interaction outcomes. Within the plant microbiome research field, unlocking the functional relationships between plants and their microbial partners is the next step to effectively using the microbiome to improve plant fitness. We propose that strategies that pair host and microbial datasets—referred to here as holo-omics—provide a powerful approach for hypothesis development and advancement in this area. We discuss several experimental design considerations and present a case study to highlight the potential for holo-omics to generate a more holistic perspective of molecular networks within the plant microbiome system. In addition, we discuss the biggest challenges for conducting holo-omics studies; specifically, the lack of vetted analytical frameworks, publicly available tools, and required technical expertise to process and integrate heterogeneous data. Finally, we conclude with a perspective on appropriate use-cases for holo-omics studies, the need for downstream validation, and new experimental techniques that hold promise for the plant microbiome research field. We argue that utilizing a holo-omics approach to characterize host-microbiome interactions can provide important opportunities for broadening system-level understandings and significantly inform microbial approaches to improving host health and fitness.

scholarly journals Sorghum Growth Promotion by Paraburkholderia tropica and Herbaspirillum frisingense: Putative Mechanisms Revealed by Genomics and Metagenomics

2020 ◽  
Vol 8 (5) ◽  
pp. 725 ◽  
Author(s):  
Eiko E. Kuramae ◽  
Stan Derksen ◽  
Thiago R. Schlemper ◽  
Maurício R. Dimitrov ◽  
Ohana Y. A. Costa ◽  
...  
Keyword(s):  
Plant Growth ◽  
Sorghum Bicolor ◽  
Plant Biomass ◽  
Growth Promotion ◽  
Genomic Analysis ◽  
Shoot Biomass ◽  
Plant Growth Promoting ◽  
Plant Hormone Signaling ◽  
Underlying Mechanisms ◽  
High Degree

Bacteria from the genera Paraburkholderia and Herbaspirillum can promote the growth of Sorghum bicolor, but the underlying mechanisms are not yet known. In a pot experiment, sorghum plants grown on sterilized substrate were inoculated with Paraburkholderia tropica strain IAC/BECa 135 and Herbaspirillum frisingense strain IAC/BECa 152 under phosphate-deficient conditions. These strains significantly increased Sorghum bicolor cultivar SRN-39 root and shoot biomass. Shotgun metagenomic analysis of the rhizosphere revealed successful colonization by both strains; however, the incidence of colonization was higher in plants inoculated with P. tropica strain IAC/BECa 135 than in those inoculated with H. frisingense strain IAC/BECa 152. Conversely, plants inoculated with H. frisingense strain IAC/BECa 152 showed the highest increase in biomass. Genomic analysis of the two inoculants implied a high degree of rhizosphere fitness of P. tropica strain IAC/BECa 135 through environmental signal processing, biofilm formation, and nutrient acquisition. Both genomes contained genes related to plant growth-promoting bacterial (PGPB) traits, including genes related to indole-3-acetate (IAA) synthesis, nitrogen fixation, nodulation, siderophore production, and phosphate solubilization, although the P. tropica strain IAC/BECa 135 genome contained a slightly more extensive repertoire. This study provides evidence that complementary mechanisms of growth promotion in Sorghum might occur, i.e., that P. tropica strain IAC/BECa 135 acts in the rhizosphere and increases the availability of nutrients, while H. frisingense strain IAC/BECa 152 influences plant hormone signaling. While the functional and taxonomic profiles of the rhizobiomes were similar in all treatments, significant differences in plant biomass were observed, indicating that the rhizobiome and the endophytic microbial community may play equally important roles in the complicated plant-microbial interplay underlying increased host plant growth.

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