scholarly journals Phenolic Profile, Toxicity, Enzyme Inhibition, In Silico Studies, and Antioxidant Properties of Cakile maritima Scop. (Brassicaceae) from Southern Portugal

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 142 ◽  
Author(s):  
Chloé Placines ◽  
Viana Castañeda-Loaiza ◽  
Maria João Rodrigues ◽  
Catarina G. Pereira ◽  
Azzurra Stefanucci ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
High Performance ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Docking Studies ◽  
In Silico Studies ◽  
Cakile Maritima ◽  
Southern Portugal ◽  
Kaempferol Glycosides ◽  
Ethanol Extracts

Cakile maritima Scop. (sea rocket) is an edible halophyte plant with several ethnomedicinal uses. This work reports the chemical profile and bioactivities of food grade extracts from sea rocket organs. Toxicity was determined on mammalian cells, and phenolic profiling and the quantitation of the main metabolites were made by high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS). Enzymatic inhibition was determined towards acetyl- and butyrylcholinesterase (AChE, BuChE), α-glucosidase, α-amylase, and tyrosinase. Docking studies were performed to tyrosinase, on the major metabolites, and samples were tested for antioxidant properties. Extracts were not toxic, were constituted mainly by flavonoids, and some compounds (roseoside and oleuropein) are here described for the first time in the species. The aerial organs’ ethanol extract had relevant activity towards 2,2-diphenyl-1-picrylhydrazyl [DPPH, half maximal inhibitory concentration (IC50) = 0.59 mg/mL], and ferric-reducing activity power (FRAP, IC50 = 0.99 mg/mL). All samples were more active towards AChE than on BuChE. The ethanol fruits’ extract inhibited α-glucosidase [2.19 mmol of equivalent of acarbose (ACAE)/g]. Samples were active against tyrosinase, especially the aerial organs’ ethanol extracts [25.9 mg of equivalent of kojic acid (KAE)/g]. Quercetin and kaempferol glycosides fit well into the enzymatic pocket of tyrosinase. Our results suggest sea rocket as a candidate to be further explored as a source of bioactive products.

scholarly journals Evaluation of Toxicity and Antioxidant Property of Cassia fistula Stem Bark Extracts in Zebrafish

2020 ◽  
Vol 10 (03) ◽  
pp. 109-115
Author(s):  
Shreya Udaya ◽  
Nishith Babu ◽  
Dechamma Pandyanda Nanjappa ◽  
Krithika Kalladka ◽  
Gunimala Chakraborty ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Antioxidant Property ◽  
Stem Bark ◽  
Oxygen Species ◽  
Cassia Fistula ◽  
Potential Applications ◽  
Ethanol Extracts ◽  
Stress Models

Abstract Objective This study was aimed at evaluating the toxicity and the antioxidant property of Cassia fistula stem bark extracts in zebrafish. Materials and Methods Crude aqueous and ethanol extracts of C. fistula stem bark were obtained following a standard solvent-based extraction method. The toxicity of these extracts on zebrafish embryonic development was determined and the LC50 values were calculated. Finally, the antioxidant property of C. fistula stem bark extracts was determined in arsenic-induced oxidative stress models of zebrafish. Results The aqueous extract of C. fistula stem bark showed a slightly larger LC50 value (213.6 ppm) compared with the ethanol extract (LC50 = 63.5 ppm), suggesting a lower toxicity of the aqueous extract. A significant reduction of reactive oxygen species (ROS) signal was observed in arsenic-exposed embryos treated with the aqueous extract, but not the ethanol extract, indicating that the antioxidant activity is present only in the aqueous extract of C. fistula stem bark. Conclusion Identification of antioxidants from natural sources is desirable because of increasing safety concerns associated with synthetic antioxidants. This study demonstrated that aqueous extract from C. fistula stem bark possesses antioxidant properties, which can be further characterized for mechanism of action and potential applications.

Phytochemical and antioxidant properties of Algerian Medicago Sativa L. Water, Ethenol and Chloroform extract

2019 ◽  
Vol 9 (3) ◽  
pp. 80-86
Author(s):  
Hanane Megdad ◽  
Samuel Chiomokhare ◽  
Ahmed M’Hamedi ◽  
Paul L Chazot
Keyword(s):  
Antioxidant Activity ◽  
Medicago Sativa ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Natural Antioxidants ◽  
Chloroform Extract ◽  
Total Phenolic ◽  
Medicago Sativa L ◽  
Ethanol Extracts

Plant natural products have a long history of being a rich source of neuroactive drugs.The total phenolic and flavonoid content, and antioxidant activity of ethanol, chloroform and aqueous extracts of AlgerianMedicagoSativaL, leaves were measured to discover new potential sources of Algerian natural antioxidants and neuroactives. All extracts were non-neurotoxic up to 1 mg/ ml for 24 h exposure. The total phenolcontent of M.Sativa extracts ranged between 38 and 69 mg GAE per g extracts. All of the extracts displayed antioxidant capacityassessed by two methods, but at different levels depending on the concentration and the extraction solvent used. The overall antioxidant potency was in the order water > chloroform> ethanol extracts, and the phenolic content exhibited a positive correlation with the antioxidant activity of M. Sativa extracts.Despite a weaker antioxidant activity, the ethanol extract elicited a calcium spike and suppressed the KCl-induced depolarisation response in neurons, indicating effect on ligand-and/or voltage gated calcium channels. In contrast, the chloroform and water extractsdisplayed no apparent direct functional effects. In conclusion, the antioxidant and functional properties related to phenolic content of Algerian Medicago Sativa L. in water and ethanol extracts, respectively.

scholarly journals Isolation of Betulinic acid from the stem bark and root of Uapaca guineensis

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Abdulrazaq Ogunmoye
Keyword(s):  
Betulinic Acid ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
2D Nmr ◽  
Stem Bark ◽  
Evergreen Forest ◽  
Spectra Analysis ◽  
Pentacyclic Triterpenoid ◽  
Ethanol Extracts ◽  
Isolated Compound

Uapaca guineensis Mull. Arg. (Euphorbiaceae) commonly called false mahogany and “Ewe akun” in the western part of Nigeria grows in humid localities, mixed evergreen forest and sea-level up to 1100 m altitude. The study was designed to isolate Betulinic acid (3?-hydroxylup-20-(29)-en-28-oic acid) which is a naturally occurring pentacyclic triterpenoid that exhibited antibacterial, antimalarial, anti-inflammatory, anthelmintic and antioxidant properties from the stem bark and root of Uapaca guineensis. The air-dried and pulverized stem bark and root of U. guineensis (0.5 kg each) were separately extracted with aqueous ethanol (80%) for 72 hour at room temperature, filtered, concentrated on rotatory evaporator at 37 oC and then stored in a desiccator. The ethanol extracts of the stem bark was partitioned successively with n-hexane, dichloromethane and methanol, while ethanol extract of the root was chromatographed directly. The dichloromethane and methanol extracts of the stem bark was also chromatographed. These results in the formation of whitish powder that are UV inactive and also gave single spot each with cerium sulphate. They are coded as DBG-D13 (25.0 mg), MBG-Q3 (6.1 mg) and ERG-E7 (20.0 mg) for dichloromethane, Methanol and Ethanol extracts respectively. The structure was unequivocally established by 1HNMR, 13CNMR, 2D-NMR, IR, UV and MS spectra analysis as well as by comparison of the spectra data with previously reported values. The dichloromethane, methanol and the ethanol extracts all yielded betulinic acid in substantial quantity. The isolated compound is a confirmation to existing information and an addition to the library of organic compounds.

scholarly journals Antiviral activity and chemical characterization of Cissus erosa (Vitaceae) ethanol extracts

Rodriguésia ◽  
2020 ◽  
Vol 71 ◽  
Author(s):  
Adriana Cotta Cardoso Reis ◽  
Hélia Maria Marques de Moura ◽  
Breno Mello Silva ◽  
Alaíde Braga de Oliveira ◽  
Geraldo Célio Brandão
Keyword(s):  
Antiviral Activity ◽  
Zika Virus ◽  
High Performance ◽  
Colorimetric Method ◽  
Chemical Characterization ◽  
Ethanol Extract ◽  
Virus Activity ◽  
Stems And Leaves ◽  
Ethanol Extracts

Abstract Cissus erosa (Vitaceae), popularly known in Brazil as Cipó-fogo, is a medicinal plant used in the treatment of warts and external ulcers. The present study aimed to evaluate the activity of stems and leaves ethanol extracts of C. erosa against the Dengue and Zika virus by the MTT colorimetric method and to carry on the phytochemical characterization of active extracts by high performance liquid chromatography coupled to mass spectrometry (UPLC-MS). Only the leaves ethanol extract showed anti-Dengue virus activity EC50 18.2 µg/ml (SI > 27.5) and low cytotoxicity for LLCMK2 cells (CC50 > 500 mg/ml). Both extracts (stems and leaves) showed anti-Zika virus activity with EC50 of 45.8 mg/ml and 82.8 mg/ml, respectively. These extracts presented CC50 of 309.2 µg/ml (leaves) and 387.6 µg/ml (stems) to Vero cells. Analysis by CCD and HPLC-DAD detected the presence of triterpenes, steroids, flavonoids and tannins. UPLC-MS analyses of these extracts, allowed the identification of the majority of flavonoids present known as vitexin, isovitexin, orientin, isoorientin and two flavones derivatives, methoxyluteolin-6(8)-C-hexosyl and luteolin-7,4’-di-O-glycosylflavone. The results of the phytochemical studies here described suggest that flavonoids and terpenoids are the substances that contribute to the antiviral activity of the ethanol extracts within this species.

In Silico ADMET and Docking Studies of Thiazolidinedione-acetic-acid Hybrids as Antidiabetics with Cardioprotection

2020 ◽  
Vol 17 (12) ◽  
pp. 1475-1484
Author(s):  
Deepanwita Maji ◽  
Subir Samanta ◽  
Vaishali M. Patil
Keyword(s):  
Acetic Acid ◽  
Amino Acid ◽  
Side Effects ◽  
In Silico ◽  
Methyl Esters ◽  
Antioxidant Properties ◽  
Docking Studies ◽  
Single Amino Acid ◽  
In Silico Admet ◽  
In Silico Studies

Background: Type-2-diabetes mellitus is associated with many side effects affecting vital body organs, especially heart. Thiazolidinediones are potent antidiabetics. Studies have proven that amino-acids and peptides promote glucose transport, have antioxidant properties, and fewer side effects, thus we designed hybrids by combining amino-acid esters and peptide esters with 2, 4 thiazolidinedione acetic acid moiety which can act as antidiabetic agent with cardioprotection properties. Methodology: In vitro ADME, toxicity, and docking studies were performed using Qikprop3.1.OSIRIS, PROTOX (Prediction of Rodent Oral Toxicity), and FlexX 2.1.3, respectively. Results: All the designed molecules belong to three sub-series, i.e. 2, 4-dioxothiazolidine-5-acetic acid single amino acid hybrid methyl esters, 2, 4-dioxothiazolidine-5-acetic acid dipeptide hybrid methyl esters and 2, 4-dioxothiazolidine-5-acetic acid tripeptide hybrid methyl esters. All molecules were non-toxic. SSMA2, SSMA14, SSMA49, and SSDM50 showed good docking scores in 2PRG and 2UV4, respectively. Conclusion: The selected in silico studies helped to design hybrids with less toxicity, target specificity with dual activity as potential anti-diabetic and cardioprotective agents.

scholarly journals Geographic Variation in the Chemical Composition and Antioxidant Properties of Phenolic Compounds from Cyclocarya paliurus (Batal) Iljinskaja Leaves

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2440 ◽  
Author(s):  
Yang Liu ◽  
Pei Chen ◽  
Mingming Zhou ◽  
Tongli Wang ◽  
Shengzuo Fang ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Geographic Origin ◽  
Cyclocarya Paliurus ◽  
Sampling Locations ◽  
Kaempferol Glycosides

Cyclocarya paliurus has been widely used as an ingredient in functional foods in China. However, the antioxidant properties of phenolic compounds and the effect of the plant origin remain unclear. The present study evaluated the geographical variation of this plant in term of its phenolic composition and antioxidant activities based on leaf materials collected from five regions. high-performance liquid chromatography (HPLC) analysis showed that there are three major components, quercetin-3-O-glucuronide, kaempferol-3-O-glucuronide, and kaempferol-3-O-rhamnoside, and their contents varied significantly among sampling locations. The investigated phenolic compounds showed substantial antioxidant activities, both in vitro and in vivo, with the highest capacity observed from Wufeng and Jinzhongshan. Correlation analysis revealed that quercetin and kaempferol glycosides might be responsible for the antioxidant activities. Our results indicate the importance of geographic origin, with sunny hours and temperature as the main drivers affecting the accumulation of C. paliurus phenolics and their antioxidant properties.

scholarly journals Optimization of polyphenols extraction process with antioxidant properties from wild Vaccinium myrtillus L. (bilberry) and Vaccinium vitis-idaea L. (lingonberry) leaves

2020 ◽  
Vol 3 (2) ◽  
pp. 149
Author(s):  
Radka Vrancheva ◽  
Ivan Ivanov ◽  
Ilian Badjakov ◽  
Ivayla Dincheva ◽  
Vasil Georgiev ◽  
...  
Keyword(s):  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Extraction Process ◽  
Vaccinium Myrtillus ◽  
Total Phenol Content ◽  
Flavonoid Content ◽  
Polyphenol Compounds ◽  
Extraction Solvent ◽  
Ethanol Extracts ◽  
Vaccinium Myrtillus L

The aim of the current study was to optimize the extraction condition of polyphenol compounds with antioxidant properties from leaves of natural grown Vaccinium myrtillus L and Vaccinium vitis-idaea L. The extractions were carried out in ultrasonic bath at 40 ºC for 20 minutes with different solvents (water, 20 % ethanol, 40 % ethanol, 60 % ethanol, 80 % ethanol and 96 % ethanol) and different hydro module of samples and solvents used (1:50, 1:100 and 1:200). The highest total phenol content in the leaves of V. myrtillus L. and V. vitis-idaea L. was found when 40% ethanol extract was used (90.50 ± 0.05 mg GAE/g DW and 96.68 ± 0.05 mg GAE/g DW, respectively). The highest total flavonoid content of the leaves of both species was obtained with 80 % ethanol as extraction solvent. The highest level of total proanthocyanidins were in the 60 % ethanol extract of V. myrtillus L. and in the 80 % ethanol extract of V. vitis-idaea L (13.12 ± 0.11 mg LE/g DW and 24.22 ± 0.21 mg LE/g DW, respectively). The highest ability to scavenge DPPH radicals possessed the 40 % ethanol extracts from the leaves of both species (693.99 ± 4.05 mM TE/g DW for V. myrtillus L. and 1083.18 ± 8.48 mM TE/g DW for V. vitis-idaea L). Data analysis showed that the maximal amount of polyphenols was extracted at a hydro-module of 1: 100. HPLC analysis revealed that the dominant phenolic acid in the leaves of V. myrtillus L was chlorogenic acid (13.45 mg/g DW), while ferulic acid (49.48 mg/g DW) was present at the highest concentration in the leaves of V. vitis-idaea L.

scholarly journals Phenolic Compounds and Antioxidant Activity of Nepeta nuda subsp. Albiflora

2020 ◽  
Vol 79 ◽  
pp. 1-8
Author(s):  
İbrahim Teber ◽  
Ercan Bursal
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Plant Sample ◽  
Plant Biochemistry ◽  
Ethanol Extracts ◽  
Biochemical Analyses

Phenolic content and antioxidant activity of Nepeta nuda subsp. albiflora Boiss. were reported in this study. The ethanol and water extracts of Nepeta nuda subsp. albiflora were prepared and used for biochemical analyses. Antioxidant capacities of the extracts were evaluated by three different in vitro bioanalytical methods including a reducing antioxidant method and two radical scavenging antioxidant methods. The water and ethanol extracts of the plant sample were found to have effective antioxidant potentials. Phenolic content of Nepeta nuda subsp. albiflora was determined by high performance liquid chromatography (HPLC). Rosmarinic acid (182.0±4.5 µg/g), apigenin (84.5±57.6 µg/g), and quercetin (44.5±62.9 µg/g) were identified as major compounds in the ethanol extract of the plant sample. This study has a potential scientific base for further studies about Nepeta nuda subsp. albiflora related to plant biochemistry and plant based pharmacological industry.

scholarly journals Phenolic Combination and Comparison of Antioxidant Activity in Three Different Alcoholic Extracts of Dracocephalum moldavica L.

2017 ◽  
Vol 5 (3) ◽  
pp. 199 ◽  
Author(s):  
Behnaz Aslanipour ◽  
Reza Heidari ◽  
Neda Farnad
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Reducing Ability ◽  
Ethanol Extracts ◽  
Phenolic And Flavonoid

Dracocephalum moldavica L. has been used as both treatment of coronary heart disorders and food supplements. In the present study, determination of total phenolic and flavonoid content in methanol, ethanol and methanol/ethanol extracts of leaf was studied in Iranian Moldavian balm for the first time. Antioxidant activity of the studied plant was also measured. The methanol extract had the highest phenolic and flavonoid content, anthocyanin, DPPH and H2O2 radical scavenging activity. The ethanol extract showed the least amount of all. The methanol/ethanol extract showed the highest amount in two oxides including nitric and superoxide radical scavenging activities; it also showed the highest Ferric Reducing Ability Power. The obtained chromatograms of the plant using High Performance Liquid Chromatography showed that the highest and the lowest found phenolic compounds were caffeic acid and vanilic acid, respectively. The results show that this plant is a suitable natural antioxidant to reduce the oxidative stress in human being.

In-vitro and In-silico analysisof anti-diabetic and anti-microbial activity of Cichorium intybus leaf extracts

2020 ◽  
Vol 16 ◽  
Author(s):  
Suganya Ramakrishnamurthy ◽  
Ganesan Singaravelu ◽  
Velmurugan Devadasan ◽  
Aruna Prakasarao
Keyword(s):  
In Silico ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Docking Studies ◽  
Human Pathogens ◽  
Leaf Extracts ◽  
Ligand Complex ◽  
Dynamic Simulations ◽  
In Silico Studies

Objective: To screen the selected phytochemicals against diabetes by docking studies in comparison with experimental analysis. Methods: Ethanol crude extract has been obtained from the leaves of C.intybus and its chemical compounds were identified using GC- MS. Docking studies were carried out for selected phytochemicals to find the binding affinity and H-bond interaction using Scrodinger suite. Dynamic simulations were carried out for protein ligand complex up to 50ns using desmond OPLS AA forcefield and α- Amylase and α- Glucosidase assay were carried for ethanolic extract to infer its inhibition. Results: Four compounds were chosen for induced fit docking based on the docking score and glide energy obtained from GLIDE-XP docking. The compounds were docked with the protein target human aldose reductase (PDB ID: 2FZD) for checking the anti-diabetic nature. The molecular dynamics simulations were carried out for the most favorable compounds and stability has been checked during the simulations. The ethanol extract exhibits significant α-amylase and α-glucosidase inhibitory activities with an IC50 value of 38µg and 88µg dry extract respectively and well compared with standard acarbose drug.The antimicrobial activity was also carried out for various extracts (Chloroform, Ethyl acetate and Ethanol) of the same (C. intybus) was screened against four selected human pathogens. Compared to other solvent extracts, ethanol and chloroform extract shows better inhibition and their minimal inhibitory concentration (MIC) value has been calculated. Conclusion: In-silico studies and in-vitro studies reveals that C.intybus plant compounds have more potent for treating diabetes

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