scholarly journals Physiological, Biochemical and Reproductive Studies on Valeriana wallichii, a Critically Endangered Medicinal Plant of the Himalayan Region Grown under In-Situ and Ex-Situ Conditions

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 131 ◽  
Author(s):  
Mohd Asgher ◽  
Susheel Verma ◽  
Nafees A. Khan ◽  
Dhiraj Vyas ◽  
Priyanka Kumari ◽  
...  
Keyword(s):  
Shikimic Acid ◽  
Natural Habitat ◽  
Himalayan Region ◽  
Ex Situ ◽  
Perennial Herb ◽  
Natural Habitats ◽  
Term Survival ◽  
The Difference ◽  
Glucose 6 Phosphate Dehydrogenase

Valeriana wallichii, a perennial herb belonging to family Valerianaceae, is an important medicinal herb of the Himalayan region. The incessant exploitation of nature for meeting the demands of the pharmaceutical industry has put unbearable pressure on its natural habitats. A study on its physiological, biochemical, growth and reproductive attributes was planned. Physiological study revealed that ex-situ (outside their natural habitat) populations faced severe stress as compared to in-situ (natural habitat) plants. The difference in the performance of these habitat plants was related to superoxide and H2O2 in the leaves. Photosynthetic attributes were increased in in-situ populations. Proline content and its biosynthetic enzymes ornithine aminotransferase, and pyrroline-5-carboxylate reductase showed an increase in ex-situ plants; proline oxidase decreased. Glucose-6-phosphate dehydrogenase, shikimic acid dehydrogenese, phenylalanine lyase, and flavonoids content showed an increment in ex-situ plants. Antioxidants enzyme superoxide dismutase, catalase, ascorbate peroxidase and reduced glutathione showed an increment in ex-situ conditions. Growth and reproductive attributes were more in ex-situ plants. The observations made are suggestive that a comprehensive conservation programme involving in-situ as well as ex-situ strategies will be effective for the conservation and long term survival of the species.

Diversity, Distribution, Indigenous Uses and Conservation of Medicinal Plants in Nargu Wildlife Sanctuary, North Western Himalaya

2015 ◽  
Vol 22 (4) ◽  
pp. 181-201
Author(s):  
Pankaj Sharma ◽  
Sher Samant ◽  
Lalit Tewari
Keyword(s):  
Medicinal Plants ◽  
Plant Species ◽  
Gastrointestinal Disease ◽  
Himalayan Region ◽  
Ex Situ ◽  
Herbal Remedies ◽  
Poor People ◽  
Wildlife Sanctuary ◽  
Medicinal Species ◽  
Natural Habitats

In the Indian Himalayan Region, the use of medicinal plants is still a tradition continued by local people or ethnic communities and Traditional Medicine Systems play an important role in daily health care of poor people. The use of plants as medicine is slowly increasing in the developed world because they have minor or no side effects. The present study was conducted in Nargu Wildlife Sanctuary of Himachal Pradesh as the total dependency of the stakeholders on nearby forests for medicinal resources. In the present study, we reported 371 plant species that are used by the people in and around Nargu WLS as medicine. Distribution of maximum species (297 spp.) occurred between 1801-2800 m zone and only 40 species were present above 3800m. Of the total medicinal species recorded, 163 spp. were native to the Himalayan region and 24 spp. native to the Himalaya and other biogeographically regions. Rests (184 spp.) were non-native. The most frequently cured disease category (108 spp.) was gastrointestinal disease. Stakeholders of the Sanctuary and surrounding villages have rich indigenous knowledge of the use of medicinal plants to maintain their primary health. Traditional herbal remedies are important and effective because many traditional uses are scientifically proven through phytochemical and pharmacological studies. However, a large number of plant species remain untested for bio-efficacy and toxicity. Such tests may reveal novel remedies that have bioprospecting potential. Moreover, the study emphasizes the need for the conservation of such precious medicinal resource of the area by managing and conserving the natural habitats, checking unscientific exploitation and by applying in-situ and various ex-situ conservation methods.

scholarly journals In situ conservation of fruit landraces

2010 ◽  
Vol 46 (Special Issue) ◽  
pp. S57-S59 ◽  
Author(s):  
F. Paprštein ◽  
J. Sedlák ◽  
V. Holubec
Keyword(s):  
Global Positioning System ◽  
Natural Habitat ◽  
In Situ Conservation ◽  
Sour Cherry ◽  
Ex Situ ◽  
The Czech Republic ◽  
Fruit Species ◽  
Global Positioning ◽  
Berry Fruits

<I>In situ </I>conservation is considered as conservation of wild biota in the natural habitat (locality). The authors extend the term to cultivated fruit species naturalised in the landscape, such as occasional spontaneous seedlings, and planted material such as old solitary trees among fields, old groves, avenues (country lanes), wind-breaks, and abandoned remnants of orchards. <I>In situ </I>conservation is also used to mark unique materials during collecting expeditions, before they will be taken as <I>ex situ </I>or proclaimed as permanent <I>in situ</I>. Important landraces found within 12 regions of the Czech Republic were registered, evaluated, and <I>in situ </I>localised by Global Positioning System (GPS). The following accessions were marked for in-situ conservation: apple (401), sweet cherry (263), pear (91), plum (42), sour cherry (27), and berry fruits (18).

scholarly journals Long-term conservation of potato genetic resources: Methods and status of conservation

2019 ◽  
pp. 717-725
Author(s):  
Jane Muthoni ◽  
Hussein Shimelis ◽  
Rob Melis
Keyword(s):  
Genetic Resources ◽  
Slow Growth ◽  
Plant Genetic Resources ◽  
Ex Situ ◽  
Growth Media ◽  
Medium Term ◽  
Natural Habitats

Plant genetic resources (PGRs) play an important role in agriculture, environment protection, cultural property and trade; they need to be conserved. There are two fundamental approaches for the conservation of PGRs: in situ and ex situ. In situ conservation is the conservation of ecosystems and natural habitats and the maintenance and recovery of viable populations of species in their natural surroundings. Ex situ preservation is the storage of seeds or plant materials under artificial conditions to maintain their long term viability and availability for use. Genebanks employ seed storage, field collections of living plants and in vitro storage (tissue culture or cryopreservation) for ex situ preservation of PGR. Storage of orthodox seeds, which are tolerant to low moisture content and low temperatures at appropriate temperature and humidity, is the most convenient ex situ conservation method. Plants that produce recalcitrant seeds or non-viable seeds are conserved in field genebanks as well as in-vitro in slow growth media for short-to-medium term and cryopreservation in liquid nitrogen at -1960C for long-term periods. Cryopreservation is very expensive and needs trained personnel; this could explain why this method is rarely used for conservation of plant genetic resources in most developing countries. Potato tubers are bulky and highly perishable; the crop is generally conserved as clones either in field genebanks (with annual replanting), in-vitro conservation in slow growth media for short-to-medium term and cryopreservation for long term. Field genebanks are expensive to maintain and the crop is exposed to many dangers; hence, cryopreservation is the only feasible method for long term conservation. However, given the high cost of cryopreservation, long-term conservation of potato genetic resources is poorly developed in most resource-poor countries leading to high rates of genetic erosion. This paper looks into the various methods that that can be applied to conserve potato genetic resources and the status of conservation of potatoes in major genebanks and some countries.

scholarly journals Origin of the Difference in the Resistivity of As-Grown Focused-Ion- and Focused-Electron-Beam-Induced Pt Nanodeposits

2009 ◽  
Vol 2009 ◽  
pp. 1-11 ◽  
Author(s):  
J. M. De Teresa ◽  
R. Córdoba ◽  
A. Fernández-Pacheco ◽  
O. Montero ◽  
P. Strichovanec ◽  
...  
Keyword(s):  
Voltage Dependence ◽  
Ion Beam ◽  
Carbon Matrix ◽  
Ex Situ ◽  
Semiconducting Materials ◽  
Dual Beam ◽  
Transmission Electron ◽  
The Difference ◽  
Main Component

We study the origin of the strong difference in the resistivity of focused-electron- and focused-Ga-ion-beam-induced deposition (FEBID and FIBID, resp.) of Pt performed in a dual beam equipment using(CH3)3Pt(CpCH3)as the precursor gas. We have performed in-situ and ex-situ resistance measurements in both types of nanodeposits, finding that the resistivity of Pt by FEBID is typically four orders of magnitude higher than Pt by FIBID. In the case of Pt by FEBID, the current-versus-voltage dependence is nonlinear and the resistance-versus-temperature behavior is strongly semiconducting, whereas Pt by FIBID shows linear current-versus-voltage dependence and only slight temperature dependence. The microstructure, as investigated by high-resolution transmission electron microscopy, consists in all cases of Pt single crystals with size about 3 nm embedded in an amorphous carbonaceous matrix. Due to the semiconducting character of the carbon matrix, which is the main component of the deposit, we propose that the transport results can be mapped onto those obtained in semiconducting materials with different degrees of doping. The different transport properties of Pt by FEBID and FIBID are attributed to the higher doping level in the case of FIBID, as given by composition measurements obtained with energy-dispersive X-ray microanalysis.

Ex situ conservation of Aconitum heterophyllum Wall.—an endangered medicinal plant of the Himalaya through mass propagation and its effect on growth and alkaloid content

2005 ◽  
Vol 3 (2) ◽  
pp. 127-135 ◽  
Author(s):  
Subedar Pandey ◽  
Rekha Kushwaha ◽  
Om Prakash ◽  
Amita Bhattacharya ◽  
P.S. Ahuja
Keyword(s):  
Ex Situ Conservation ◽  
Natural Habitat ◽  
Hot Water ◽  
Alkaloid Content ◽  
Ex Situ ◽  
Endangered Medicinal Plant ◽  
Aconitum Heterophyllum ◽  
Total Alkaloid Content ◽  
The Himalaya

The endangered alpine plant Aconitum heterophyllum was conserved under ex situ conditions (greenhouse and open) of Palampur by overcoming seed dormancy and plant establishment through hot water treatment (40–60 °C for 30–120 s) of seeds. High seed germination (88 and 76%) was obtained when seeds were treated with hot water at 45 and 50 °C for 90 s. Correlation studies also revealed that treatment enhanced vegetative growth and reproductive yield of the plants. The total alkaloid content of the roots and rhizomes of the plants growing under ex situ conditions was lower than the ones collected from the natural habitat in the 1-year-old plants. However, in the 2-year-old plants, it was almost at par with the ones collected from nature. The life cycle of the plants growing under ex situ conditions also did not vary largely from the plants growing under in situ conditions. Therefore, the present study indicates the successful adaptation of A. heterophyllum plants in conditions other than their natural habitat and hence its potential for sustainable commercialization.

scholarly journals Saussurea species in Indian Himalayan Region: diversity, distribution and indigenous uses

2010 ◽  
Vol 1 (1) ◽  
pp. 9 ◽  
Author(s):  
Jitendra Singh Butola ◽  
Sher S. Samant
Keyword(s):  
Raw Materials ◽  
Himalayan Region ◽  
Ex Situ ◽  
Natural Habitats ◽  
Habitat Specificity ◽  
Important Species ◽  
Study Region ◽  
Indian Himalayan Region ◽  
Native Communities ◽  
Indigenous Uses

In spite of the high economic value of the Saussurea species in the Indian Himalayan Region (IHR), the potential of most of the species is yet to be investigated. Therefore, an attempt has been made to study the diversity, distribution, habitat preference, nativity, endemism, status and indigenous uses of Saussurea species in the IHR. A total of 62 species were recorded from the IHR; of these, 37 species were native to the Himalayan region, 8 were endemic and 21 were near endemic to the IHR. Twenty-seven of the 28 species that were known to have indigenous uses also had medicinal value and are used for the treatment of various diseases/ailments. Many species, e.g., S. affinis (Ganga Mula*), S. auriculata (Pachak Kut), S. bracteata (Prerak Mul), S. costus (Kuth), S. gossypiphora (Kasturi Kamal) and S. obvallata (Brahm Kamal), have multiple uses. The genus showed high habitat specificity in that 16 species were recorded to be restricted to one or two habitats only. As many as 44 species were identified as rare in the study region. Considering the high industrial demand for raw materials and the endangered status of S. costus, S. gossypiphora, S. obvallata and S. simpsoniana (Fen Kamal), these species should be prioritized for conservation (in situ and ex situ) throughout the IHR. Population assessment of the rare-endangered, native, endemic and economically important species using standard ecological methods has been suggested for the quantification of the existing stock of these species in their natural habitats. Further, phyto-chemical investigations for the identification of active ingredients are suggested. Propagation and cultivation techniques are lacking for most of the species of Saussurea except for S. costus, S. obvallata and S. medusa (Snow Lotus). Furthermore, the native communities need to be sensitized to the sustainable use and conservation value of the species in this genus. *Local names given in parentheses throughout are in the Pahari language, spoken by native communities in the Himalayan region.

scholarly journals Seed and biological productivity of Prunella vulgaris L. in situ and ex situ

2018 ◽  
Vol 11 ◽  
pp. 00004
Author(s):  
Irina Barsukova ◽  
Alexei Astashenkov
Keyword(s):  
Growth Conditions ◽  
Ex Situ ◽  
Optimal Conditions ◽  
Biological Productivity ◽  
Prunella Vulgaris ◽  
Seed Productivity ◽  
Natural Habitats ◽  
Productivity Increase ◽  
In The Wild

Reproductive biology and biological productivity of Prunella vulgaris L. were studied in natural growth conditions in Khakasiya and under introduction. It was established that in the wild a potential seed productivity of the species varied from 85,4 to 160,3 it./ a shoot, a real one – from 73,6 to 149,2 it.; erem germination under optimal conditions reached 90,5%. Under introduction the indicators of seed productivity increase by 2-5 times, of erem germination – by 1,5 times. Biological productivity of individuals in cultivation is by 5-10 times higher compared to that in natural habitats.

scholarly journals Survival of and In Situ Gene Expression by Vibrio vulnificus at Varying Salinities in Estuarine Environments

2007 ◽  
Vol 74 (1) ◽  
pp. 182-187 ◽  
Author(s):  
Melissa K. Jones ◽  
Elizabeth Warner ◽  
James D. Oliver
Keyword(s):  
Gene Expression ◽  
Vibrio Vulnificus ◽  
Natural Habitat ◽  
Term Survival ◽  
Long Term Survival ◽  
Wide Range ◽  
Opportunistic Human Pathogen

ABSTRACT The opportunistic human pathogen Vibrio vulnificus survives in a wide range of ecological environments, which demonstrates its ability to adapt to highly variable conditions. Survival and gene expression under various conditions have been extensively studied in vitro; however, little work has been done to evaluate this bacterium in its natural habitat. Therefore, this study monitored the long-term survival of V. vulnificus in situ and simultaneously evaluated the expression of stress (rpoS, relA, hfq, and groEL) and putative virulence (vvpE, smcR, viuB, and trkA) genes at estuarine sites of varying salinity. Additionally, the survival and gene expression of an rpoS and an oxyR mutant were examined under the same conditions. Differences between the sampling sites in the long-term survival of any strain were not seen. However, differences were seen in the expression of viuB, trkA, and relA but our findings differed from what has been previously shown in vitro. These results also routinely demonstrated that genes required for survival under in vitro stress or host conditions are not necessarily required for survival in the water column. Overall, this study highlights the need for further in situ evaluation of this bacterium in order to gain a true understanding of its ecology and how it relates to its natural habitat.

scholarly journals Phenotypic diversity assessment within a major ex situ collection of wild endemic coffees in Madagascar

2020 ◽  
Vol 126 (5) ◽  
pp. 849-863
Author(s):  
Aurore Rimlinger ◽  
Nathalie Raharimalala ◽  
Véronique Letort ◽  
Jean-Jacques Rakotomalala ◽  
Dominique Crouzillat ◽  
...  
Keyword(s):  
Phenotypic Diversity ◽  
Phylogenetic Signal ◽  
Natural Habitat ◽  
Ex Situ ◽  
Dry Matter Content ◽  
Natural Habitats ◽  
Leaf Mass ◽  
Content Ratio ◽  
Diversity Assessment ◽  
Ex Situ Collection

Abstract Background and Aims Like other clades, the Coffea genus is highly diversified on the island of Madagascar. The 66 endemic species have colonized various environments and consequently exhibit a wide diversity of morphological, functional and phenological features and reproductive strategies. The trends of interspecific trait variation, which stems from interactions between genetically defined species and their environment, still needed to be addressed for Malagasy coffee trees. Methods Data acquisition was done in the most comprehensive ex situ collection of Madagascan wild Coffea. The structure of endemic wild coffees maintained in an ex situ collection was explored in terms of morphological, phenological and functional traits. The environmental (natural habitat) effect was assessed on traits in species from distinct natural habitats. Phylogenetic signal (Pagel’s λ, Blomberg’s K) was used to quantify trait proximities among species according to their phylogenetic relatedness. Key Results Despite the lack of environmental difference in the ex situ collection, widely diverging phenotypes were observed. Phylogenetic signal was found to vary greatly across and even within trait categories. The highest values were exhibited by the ratio of internode mass to leaf mass, the length of the maturation phase and leaf dry matter content (ratio of dry leaf mass to fresh leaf mass). By contrast, traits weakly linked to phylogeny were either constrained by the original natural environment (leaf size) or under selective pressures (phenological traits). Conclusions This study gives insight into complex patterns of trait variability found in an ex situ collection, and underlines the opportunities offered by living ex situ collections for research characterizing phenotypic variation.

Study on the difference between in-situ and ex-situ catalytic pyrolysis of oily sludge

2021 ◽  
Author(s):  
Qian He ◽  
Shengxiong Huang ◽  
Wei Luo ◽  
Yifeng Su ◽  
Mao Xia ◽  
...  
Keyword(s):  
Catalytic Pyrolysis ◽  
Ex Situ ◽  
Oily Sludge ◽  
The Difference
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