scholarly journals Early Detection of Sage (Salvia officinalis L.) Responses to Ozone Using Reflectance Spectroscopy

Plants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 346 ◽  
Author(s):  
Marchica ◽  
Loré ◽  
Cotrozzi ◽  
Lorenzini ◽  
Nali ◽  
...  

Advancements in techniques to rapidly and non-destructively detect the impact of tropospheric ozone (O3) on crops are required. This study demonstrates the capability of full-range (350–2500 nm) reflectance spectroscopy to characterize responses of asymptomatic sage leaves under an acute O3 exposure (200 ppb for 5 h). Using partial least squares regression, spectral models were developed for the estimation of several traits related to photosynthesis, the oxidative pressure induced by O3, and the antioxidant mechanisms adopted by plants to cope with the pollutant. Physiological traits were well predicted by spectroscopic models (average model goodness-of-fit for validation (R2): 0.65–0.90), whereas lower prediction performances were found for biochemical traits (R2: 0.42–0.71). Furthermore, even in the absence of visible symptoms, comparing the full-range spectral profiles, it was possible to distinguish with accuracy plants exposed to charcoal-filtered air from those exposed to O3. An O3 effect on sage spectra was detectable from 1 to 5 h from the beginning of the exposure, but ozonated plants quickly recovered after the fumigation. This O3-tolerance was confirmed by trends of vegetation indices and leaf traits derived from spectra, further highlighting the capability of reflectance spectroscopy to early detect the responses of crops to O3.

Agronomy ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1895
Author(s):  
José Ramón Rodríguez-Pérez ◽  
Víctor Marcelo ◽  
Dimas Pereira-Obaya ◽  
Marta García-Fernández ◽  
Enoc Sanz-Ablanedo

Visible, near, and shortwave infrared (VIS-NIR-SWIR) reflectance spectroscopy, a cost-effective and rapid means of characterizing soils, was used to predict soil sample properties for four vineyards (central and north-western Spain). Sieved and air-dried samples were measured using a portable spectroradiometer (350–2500 nm) and compared for pistol grip (PG) versus contact probe (CP) setups. Raw data processed using standard normal variate (SVN) and detrending transformation (DT) were grouped into four subsets (VIS: 350–700 nm; NIR: 701–1000 nm; SWIR: 1001–2500 nm; and full range: 350–2500 nm) in order to identify the most suitable range for determining soil characteristics. The performance of partial least squares regression (PLSR) models in predicting soil properties from reflectance spectra was evaluated by cross-validation. The four spectral subsets and transformed reflectances for each setup were used as PLSR predictor variables. The best performing PLSR models were obtained for pH, electrical conductivity, and phosphorous (R2 values above 0.92), while models for sand, nitrogen, and potassium showed moderately good performances (R2 values between 0.69 and 0.77). The SWIR subset and SVN + DT processing yielded the best PLSR models for both the PG and CP setups. VIS-NIR-SWIR reflectance spectroscopy shows promise as a technique for characterizing vineyard soils for precision viticulture purposes. Further studies will be carried out to corroborate our findings.


2020 ◽  
Vol 21 (17) ◽  
pp. 6441 ◽  
Author(s):  
Lorenzo Cotrozzi ◽  
Giacomo Lorenzini ◽  
Cristina Nali ◽  
Elisa Pellegrini ◽  
Vincenzo Saponaro ◽  
...  

High-throughput and large-scale measurements of chlorophyll a fluorescence (ChlF) are of great interest to investigate the photosynthetic performance of plants in the field. Here, we tested the capability to rapidly, precisely, and simultaneously estimate the number of pulse-amplitude-modulation ChlF parameters commonly calculated from both dark- and light-adapted leaves (an operation which usually takes tens of minutes) from the reflectance of hyperspectral data collected on light-adapted leaves of date palm seedlings chronically exposed in a FACE facility to three ozone (O3) concentrations (ambient air, AA; target 1.5 × AA O3, named as moderate O3, MO; target 2 × AA O3, named as elevated O3, EO) for 75 consecutive days. Leaf spectral measurements were paired with reference measurements of ChlF, and predictive spectral models were constructed using partial least squares regression. Most of the ChlF parameters were well predicted by spectroscopic models (average model goodness-of-fit for validation, R2: 0.53–0.82). Furthermore, comparing the full-range spectral profiles (i.e., 400–2400 nm), it was possible to distinguish with high accuracy (81% of success) plants exposed to the different O3 concentrations, especially those exposed to EO from those exposed to MO and AA. This was possible even in the absence of visible foliar injury and using a moderately O3-susceptible species like the date palm. The latter view is confirmed by the few variations of the ChlF parameters, that occurred only under EO. The results of the current study could be applied in several scientific fields, such as precision agriculture and plant phenotyping.


Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3454 ◽  
Author(s):  
Eduardo D. Munaiz ◽  
Philip A. Townsend ◽  
Michael J. Havey

Epicuticular waxes on the surface of plant leaves are important for the tolerance to abiotic stresses and plant–parasite interactions. In the onion (Allium cepa L.), the variation for the amounts and types of epicuticular waxes is significantly associated with less feeding damage by the insect Thrips tabaci (thrips). Epicuticular wax profiles are measured using used gas chromatography mass spectrometry (GCMS), which is a labor intensive and relatively expensive approach. Biochemical spectroscopy is a non-destructive tool for measurement and analysis of physiological and chemical features of plants. This study used GCMS and full-range biochemical spectroscopy to characterize epicuticular waxes on seven onion accessions with visually glossy (low wax), semi-glossy (intermediate wax), or waxy (copious wax) foliage, as well as a segregating family from the cross of glossy and waxy onions. In agreement with previous studies, GCMS revealed that the three main waxes on the leaves of a wild type waxy onion were the ketone hentriacontanone-16 (H16) and fatty alcohols octacosanol-1 (Oct) and triacontanol-1 (Tri). The glossy cultivar “Odourless Greenleaf” had a unique phenotype with essentially no H16 and Tri and higher amounts of Oct and the fatty alcohol hexacosanol-1 (Hex). Hyperspectral reflectance profiles were measured on leaves of the onion accessions and segregating family, and partial least-squares regression (PLSR) was utilized to generate a spectral coefficient for every wavelength and prediction models for the amounts of the three major wax components. PLSR predictions were robust with independent validation coefficients of determination at 0.72, 0.70, and 0.42 for H16, Oct, and Tri, respectively. The predicted amounts of H16, Oct, and Tri are the result of an additive effect of multiple spectral features of different intensities. The variation of reflectance for H16, Oct, and Tri revealed unique spectral features at 2259 nm, 645 nm, and 730 nm, respectively. Reflectance spectroscopy successfully revealed a major quantitative trait locus (QTL) for amounts of H16, Oct, and Tri in the segregating family, agreeing with previous genetic studies. This study demonstrates that hyperspectral signatures can be used for non-destructive measurement of major waxes on onion leaves as a basis for rapid plant assessment in support of developing thrips-resistant onions.


2019 ◽  
Vol 11 (4) ◽  
pp. 398 ◽  
Author(s):  
Haidi Abdullah ◽  
Roshanak Darvishzadeh ◽  
Andrew Skidmore ◽  
Marco Heurich

In this study, the early stage of European spruce bark beetle (Ips typographus, L.) infestation (so-called green attack) is investigated using Landsat-8 optical and thermal data. We conducted an extensive field survey in June and the beginning of July 2016, to collect field data measurements from several infested and healthy trees in the Bavarian Forest National Park (BFNP), Germany. In total, 157 trees were selected, and leaf traits (i.e. stomatal conductance, chlorophyll fluorescence, and water content) were measured. Three Landsat-8 images from May, July, and August 2016 were studied, representing an early stage, advanced stage, and post-infestation, respectively. Spectral vegetation indices (SVIs) sensitive to the measured traits were calculated from the optical domain (VIS, NIR, and SWIR), and canopy surface temperature (CST) was calculated from the thermal infrared band using the mono-window algorithm. The leaf traits were used to examine the impact of bark beetle infestation on the infested trees and to explore the link between these traits and remote sensing data (CST and SVIs). The differences between healthy and infested samples regarding measured leaf traits were assessed using Student’s t test. The relative importance of the CST and SVIs for estimating measured leaf traits was evaluated based on the variable importance in projection (VIP) obtained from the partial least squares regression (PLSR) analysis. A temporal comparison was then made for SVIs with a VIP > 1, including CST, using statistical significance tests. The clustering method using a principal components analysis (PCA) was used to examine visually how well the two groups of sample plots (healthy and infested) are separated in 2-D space based on principal component scores. Finally, linear regression (LR) was used to generate the leaf traits maps using the SVI that have highest VIP score and then used to produce a stress map for the study area. The results revealed that all measured leaf traits were significantly different (p < 0.05) between healthy versus infested samples. Moreover, the study showed that CST was superior to the SVIs in detecting subtle canopy changes due to bark beetle infestation for the three months considered in this study. The results showed that CST is an essential variable for estimating measured leaf traits with VIP > 1, improving the results of clustering when used with other SVIs. Likewise, the stress map produced by CST and leaf traits well presented the infestation areas at the green attacked stage. The new insight offered by this study is that the stress induced by the early stage of bark beetle infestation is more pronounced by Landsat-8 thermal bands than the SVIs calculated from its optical bands. The potential of CST in detecting the green attack stage would have positive implications for forest practice.


Agronomy ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 427
Author(s):  
Ana Belén González-Fernández ◽  
Enoc Sanz-Ablanedo ◽  
Víctor Marcelo Gabella ◽  
Marta García-Fernández ◽  
José Ramón Rodríguez-Pérez

Water status controls plant physiology and is key to managing vineyard grape quality and yield. Water status is usually estimated by leaf water potential (LWP), which is measured using a pressure chamber; however, this method is difficult, time-consuming, and error-prone. While traditional spectral methods based on leaf reflectance are faster and non-destructive, most are based on vegetation indices derived from satellite imagery (and so only take into account discrete bandwidths) and do not take full advantage of modern hyperspectral sensors that capture spectral reflectance for thousands of wavelengths. We used partial least squares regression (PLSR) to predict LWP from reflectance values (wavelength 350–2500 nm) captured with a field spectroradiometer. We first identified wavelength ranges that minimized regression error. We then tested several common data pre-processing methods to analyze the impact on PLSR prediction precision, finding that derivative pre-processing increased the determination coefficients of our models and reduced root mean squared error (RMSE). The models fitted with raw data obtained their best results at around 1450 nm, while the models with derivative pre-processed achieved their best estimates at 826 nm and 1520 nm.


2012 ◽  
Vol 61 (2) ◽  
pp. 277-290 ◽  
Author(s):  
Ádám Csorba ◽  
Vince Láng ◽  
László Fenyvesi ◽  
Erika Michéli

Napjainkban egyre nagyobb igény mutatkozik olyan technológiák és módszerek kidolgozására és alkalmazására, melyek lehetővé teszik a gyors, költséghatékony és környezetbarát talajadat-felvételezést és kiértékelést. Ezeknek az igényeknek felel meg a reflektancia spektroszkópia, mely az elektromágneses spektrum látható (VIS) és közeli infravörös (NIR) tartományában (350–2500 nm) végzett reflektancia-mérésekre épül. Figyelembe véve, hogy a talajokról felvett reflektancia spektrum információban nagyon gazdag, és a vizsgált tartományban számos talajalkotó rendelkezik karakterisztikus spektrális „ujjlenyomattal”, egyetlen görbéből lehetővé válik nagyszámú, kulcsfontosságú talajparaméter egyidejű meghatározása. Dolgozatunkban, a reflektancia spektroszkópia alapjaira helyezett, a talajok ösz-szetételének meghatározását célzó módszertani fejlesztés első lépéseit mutatjuk be. Munkánk során talajok szervesszén- és CaCO3-tartalmának megbecslését lehetővé tévő többváltozós matematikai-statisztikai módszerekre (részleges legkisebb négyzetek módszere, partial least squares regression – PLSR) épülő prediktív modellek létrehozását és tesztelését végeztük el. A létrehozott modellek tesztelése során megállapítottuk, hogy az eljárás mindkét talajparaméter esetében magas R2értéket [R2(szerves szén) = 0,815; R2(CaCO3) = 0,907] adott. A becslés pontosságát jelző közepes négyzetes eltérés (root mean squared error – RMSE) érték mindkét paraméter esetében közepesnek mondható [RMSE (szerves szén) = 0,467; RMSE (CaCO3) = 3,508], mely a reflektancia mérési előírások standardizálásával jelentősen javítható. Vizsgálataink alapján arra a következtetésre jutottunk, hogy a reflektancia spektroszkópia és a többváltozós kemometriai eljárások együttes alkalmazásával, gyors és költséghatékony adatfelvételezési és -értékelési módszerhez juthatunk.


Author(s):  
Eman Al-erqi ◽  
◽  
Mohd Lizam Mohd Diah ◽  
Najmaddin Abo Mosali ◽  
◽  
...  

This study seeks to address the impact of service quality affecting international student's satisfaction towards loyalty tothe Universiti Tun Hussein Onn Malaysia(UTHM). The aim of thestudy is to develop relationship between service quality factor and loyalty to the university from the international students’ perspectives. The study adopted quantitative approach where data was collected through questionnaire survey and analysed statistically. A total of 246 responses were received and found to be valid. The model was developed and analysed using AMOS-SEM software. Confirmatory factor analysis (CFA) function of the software was to assessed the measurement models and found that all the models achieved goodness of fit. Then path analysis function was used to assessed structural model and found that service qualityfactors have a significant effect on the students’ satisfaction and thus affecting the loyaltyto the university. Hopefully the outcome form this study will benefit the university in providing services especially to the international students.


2007 ◽  
Vol 21 (3) ◽  
pp. 245-263 ◽  
Author(s):  
Elizabeth K. Keating ◽  
Eric S. Berman

The Government Accounting Standards Board (GASB) recently released Statement No. 45, Accounting and Financial Reporting by Employers for Post-Employment Benefits Other Than Pensions and its companion Statement No. 43 for pooled stand-alone health care plans, which will profoundly affect American governmental finance. The goal of this article is to encourage governments to consider carefully a full range of options in funding and restructuring other post-employment benefits (OPEB). This article will review Statement No. 45's potential impact on governments and review existing disclosures in financial reports as well as bond offering statements. The article will discuss the statement's impact on budgets and governmental operations, including collective bargaining. Funding options under Statement No. 45 will be detailed, including the advantages and disadvantages of irrevocable trusts and OPEB bonds. The article will also discuss the impact of Medicare Part D subsidies received by governments, as well as the bond rating implications of policy decisions surrounding OPEB. As the largest government entities are just now implementing GASB Statement No. 45, estimates of the magnitude of unfunded OPEB liabilities are limited as are the strategies likely to be adopted to cover these obligations. This article offers a summary of the unfunded OPEB liabilities reported by states and major cities and suggests some measures for assessing the ability of these entities to address these costs.


2021 ◽  
Vol 13 (14) ◽  
pp. 7637
Author(s):  
Taekyoung Lee ◽  
Jieun Cha ◽  
Sohyun Sung

Trees’ ability to capture atmospheric Particular Matter (PM) is related to morphological traits (shape, size, and micro-morphology) of the leaves. The objectives of this study were (1) to find out whether cluster pattern of the leaves is also a parameter that affects trees’ PM capturing performance and (2) to apply the cluster patterns of the leaves on architectural surfaces to confirm its impact on PM capturing performance. Two series of chamber experiments were designed to observe the impact of cluster patterns on PM capturing performance whilst other influential variables were controlled. First, we exposed synthetic leaf structures of different cluster patterns (a large and sparsely arranged cluster pattern and a small and densely arranged cluster pattern) to artificially generated PM in a chamber for 60 min and recorded the changing levels of PM2.5 and PM10 every minute. The results confirmed that the small and densely arranged cluster pattern has more significant effect on reducing PM2.5 and PM10 than the large and sparsely arranged cluster pattern. Secondly, we created three different types of architectural surfaces mimicking the cluster patterns of the leaves: a base surface, a folded surface, and a folded and porous surface. The surfaces were also exposed to artificially generated PM in the chamber and the levels of PM2.5 and PM10 were recorded. The results confirmed that the folded and porous surface has a more significant effect on reducing PM2.5 and PM10 than other surfaces. The study has confirmed that the PM capturing performance of architectural surfaces can be improved by mimicking cluster pattern of the leaves.


2014 ◽  
Vol 15 (4) ◽  
pp. 1517-1531 ◽  
Author(s):  
Gerhard Smiatek ◽  
Harald Kunstmann ◽  
Andreas Heckl

Abstract The impact of climate change on the future water availability of the upper Jordan River (UJR) and its tributaries Dan, Snir, and Hermon located in the eastern Mediterranean is evaluated by a highly resolved distributed approach with the fifth-generation Pennsylvania State University–NCAR Mesoscale Model (MM5) run at 18.6- and 6.2-km resolution offline coupled with the Water Flow and Balance Simulation Model (WaSiM). The MM5 was driven with NCEP reanalysis for 1971–2000 and with Hadley Centre Coupled Model, version 3 (HadCM3), GCM forcings for 1971–2099. Because only one regional–global climate model combination was applied, the results may not give the full range of possible future projections. To describe the Dan spring behavior, the hydrological model was extended by a bypass approach to allow the fast discharge components of the Snir to enter the Dan catchment. Simulation results for the period 1976–2000 reveal that the coupled system was able to reproduce the observed discharge rates in the partially karstic complex terrain to a reasonable extent with the high-resolution 6.2-km meteorological input only. The performed future climate simulations show steadily rising temperatures with 2.2 K above the 1976–2000 mean for the period 2031–60 and 3.5 K for the period 2070–99. Precipitation trends are insignificant until the middle of the century, although a decrease of approximately 12% is simulated. For the end of the century, a reduction in rainfall ranging between 10% and 35% can be expected. Discharge in the UJR is simulated to decrease by 12% until 2060 and by 26% until 2099, both related to the 1976–2000 mean. The discharge decrease is associated with a lower number of high river flow years.


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