scholarly journals Growth and Physiology of Two Psammophytes to Precipitation Manipulation in Horqin Sandy Land, Eastern China

Plants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 244
Author(s):  
Juanli Chen ◽  
Xueyong Zhao ◽  
Xinping Liu ◽  
Yaqiu Zhang ◽  
Yayong Luo ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Drought Stress ◽  
Stress Resistance ◽  
Proline Content ◽  
Free Proline ◽  
Horqin Sandy Land ◽  
Community Succession ◽  
Sandy Land ◽  
Mda Content ◽  
Precipitation Reduction

The availability of water is the critical factor driving plant growth, physiological responses, population and community succession in arid and semiarid regions, thus a precipitation addition-reduction platform with five experimental treatments, was established to explore the growth and physiology of two psammophytes (also known as psammophiles) to precipitation manipulation in Horqin Sandy Land. Changes in coverage and density were measured, and antioxidant enzymes and osmoregulatory substances in both of the studied species were determined. Investigation results showed that the average vegetation coverage increased with an increasing precipitation, and reached a maximum in July. Under the −60% precipitation treatment, Tribulus terrestris accounted for a large proportion of the area, but Bassia dasyphylla was the dominant species in the +60% treatment. T. terrestris was found to have higher a drought stress resistance than B. dasyphylla. From days 4 to 7 after rainfall, B. dasyphylla under precipitation reduction showed obvious water stress. The malondialdehyde (MDA) content of B. dasyphylla was higher than that of T. terrestris, but that of B. dasyphylla had the lower relative water content (RWC). The MDA content in the precipitation reduction treatments of the two studied species was higher than that in the precipitation addition treatments from days 4 to 10. Peroxidase (POD) and superoxide dismutase (SOD) activity and the soluble proteins and free proline content of T. terrestris were higher than those of B. dasyphylla. The free proline content of T. terrestris and B. dasyphylla increased with increasing drought stress. Our data illustrated that T. terrestris had a higher drought stress resistance than B. dasyphylla, which was correlated with the augmentation of some antioxidant enzymes and osmoregulatory substance. The adaptive mechanism provides solid physiological support for an understanding of psammophyte adaptation to drought stress, and of community succession or species manipulation for desertified land restoration.

Study of Uranium Accumulation Mechanism and Physiological Responses of Eichhornia crassipes and Pistia stratiotes

2014 ◽  
Vol 1073-1076 ◽  
pp. 142-146
Author(s):  
Shi You Li ◽  
Qing Peng Wei ◽  
Shui Bo Xie ◽  
Ying Jiu Liu ◽  
Zheng Yan
Keyword(s):  
Antioxidant Enzymes ◽  
Proline Content ◽  
Pistia Stratiotes ◽  
Free Proline ◽  
Concentration Effects ◽  
Hydroponic Experiment ◽  
Accumulation Mechanism ◽  
Mda Content ◽  
Uranium Accumulation ◽  
Accumulation Ability

A hoagland solution hydroponic experiment was carried out to study the short-duration (8 d) accumulation ability of uranium byEichhornia crassipesandPistia stratiotesunder different uranium concentration. Effects on antioxidant enzymes (SOD, CAT, POD) activity, malondialdehyde (MDA) and free proline content ofE.crassipesandP.stratiotesunder uranium stress were discussed. The results show the activity of antioxidant enzymes is increased under 0.1~1 mg·L-1uranium stress, MDA content is at lower level indicated that antioxidant enzymes play an important role in alleviating lipid peroxidation induced by uranium stress. Under 5mg·L-1uranium stress, the antioxidant enzymes activity ofE.crassipesis at higher level and MDA content is lower,nevertheless, theP.stratiotesis just the opposite, indicating thatE.crassipestolerated higher concentrations thanP.stratiotes. The activity of antioxidant enzymes is inhibited under higher concentration (20 mg·L-1and 50 mg·L-1) uranium stress. As an important osmoregulation material, free proline content of both plants is more than the contrast.

Evaluation of Azadirachtin on Arthrospira plantensis Gomont growth parameters and antioxidant enzymes

2020 ◽  
Vol 56 ◽  
pp. 8
Author(s):  
Hatice Tunca ◽  
Ali Doğru ◽  
Feray Köçkar ◽  
Burçin Önem ◽  
Tuğba Ongun Sevindik
Keyword(s):  
Enzyme Activity ◽  
Growth Parameters ◽  
Arthrospira Platensis ◽  
Proline Content ◽  
Enzyme Analysis ◽  
Free Proline ◽  
Sod Activity ◽  
Pesticide Pollution ◽  
Antioxidant Parameters ◽  
Mda Content

Azadirachtin (Aza) used as insecticide due to inhibiting growth of insects and preventing them from feeding on plants. To understand the effects of contamination of this insecticide on phototrophs, and to determine the responses of these organisms against these insecticides are extremely important in understanding how the ecosystem is affected. In this study, chlorophyll-a amount, OD 560 and antioxidant parameters (total SOD, APX, GR, Proline, MDA and H2O2) were determined in order to understand the effect of Aza on Arthrospira platensis Gomont. Aza was applied between 0–20 μg mL−1 concentrations for 7 days in the study. Enzyme analysis was conducted at the end of the 7th day. There was a statistically significant decrease in the absorbance of OD560 and the chlorophyll-a content in A. platensis cultures exposed to the Aza (0–20 μg mL−1) during 7 days due to the increase in pesticide levels. SOD activity decreased at 8, 16 and 20 μg mL−1 concentrations; GR enzyme activity showed a significant decrease compared to the control at a concentration of 20 μg mL−1. APX activity did not change significantly compared to control. The MDA content increased significantly at 16 and 20 μg mL−1 concentrations. The H2O2 content significantly increased at 12, 16 and 20 μg mL−1 concentrations (p < 0.05) while the free proline content decreased at 4 μg mL−1 concentration (p < 0.05). As a result, regarding the Aza concentrations used in this study may be a step to prevent pesticide pollution in the environment.

Penconazole and calcium ameliorate drought stress in canola by upregulating the antioxidative enzymes

2020 ◽  
Vol 47 (9) ◽  
pp. 825 ◽  
Author(s):  
Maryam Rezayian ◽  
Vahid Niknam ◽  
Hassan Ebrahimzadeh
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Antioxidative Enzymes ◽  
Proline Content ◽  
Fresh Weight ◽  
Negative Effects ◽  
Mda Content ◽  
Effects Of Drought

The aim of this research was to gauge the alternations in the lipid peroxidation and antioxidative enzyme activity in two cultivars (cv. RGS003 and cv. Sarigol) of canola under drought stress and drought tolerance amelioration by penconazole (PEN) and calcium (Ca). Plants were treated with different polyethylene glycol (PEG) concentrations (0, 5, 10 and 15%) without or with PEN (15 mg L–1) and Ca (15 mM). The Ca treatment prevented the negative effects of drought on fresh weight (FW) in RGS003 and Sarigol at 5 and 15% PEG respectively. Ca and PEN/Ca treatments caused significant induction in the proline content in Sarigol at 15% PEG; the latter treatment was accompanied by higher glycine betaine (GB), lower malondialdehyde (MDA) and growth recovery. Hydrogen peroxide (HO2) content in Sarigol was proportional to the severity of drought stress and all PEN, Ca and PEN/Ca treatments significantly reduced the H2O2 content. PEN and PEN/Ca caused alleviation of the drought-induced oxidative stress in RGS003. RGS003 cultivar exhibited significantly higher antioxidative enzymes activity at most levels of drought, which could lead to its drought tolerance and lower MDA content. In contrast to that of Sarigol, the activity of catalase and superoxide dismutase (SOD) increased with Ca and PEN/Ca treatments in RGS003 under low stress. The application of PEN and Ca induced significantly P5CS and SOD expression in RGS003 under drought stress after 24 h. Overall, these data demonstrated that PEN and Ca have the ability to enhance the tolerance against the drought stress in canola plants.

Effects of polycyclic aromatic hydrocarbons exposure on antioxidant system activities and proline content in Kandelia candel

2011 ◽  
Vol 40 (3) ◽  
Author(s):  
Hui Song ◽  
You-Shao Wang ◽  
Cui-Ci Sun ◽  
Mei-Lin Wu ◽  
Ya-Lan Peng ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Antioxidant System ◽  
Aromatic Hydrocarbons ◽  
Inverse Correlation ◽  
Proline Content ◽  
Kandelia Candel ◽  
Significant Inverse Correlation ◽  
Polycyclic Aromatic ◽  
Mda Content ◽  
Different Levels

AbstractThe antioxidant system effects of Kandelia candel were investigated under four different levels of PAH stress. The activities of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD), the responses to the change of malondialdehyde (MDA) contents and the accumulation of proline in K. candel were determined. Our results suggested that the activities of SOD, CAT, POD increased significantly in leaves and roots of K. candel (p≤0.05) with the increase of the external PAH concentrations, while in stems, the activities of these antioxidant enzymes were all significantly inhibited (p≤0.01). We also observed an increase of MDA in leaves, stems and roots, and an obvious correlation between MDA content and PAH concentrations in three locations, which showed that the change of MDA content could be used as a biomarker of K. candel under PAH stress. The proline content was found remarkably enhanced in leaves, stems and roots. However, a significant inverse correlation was observed between the proline content and SOD (r=−0.99, p≤0.01), POD (r=−0.95, p≤0.05) activities in stems. This study suggested that the antioxidative system of K. candel has an obvious organ-dependent feature when exposed to PAH contamination as revealed by discriminant analysis (DA).

scholarly journals Ecophysiological Responses of Calcicole Cyclobalanopsis glauca (Thunb.) Oerst. to Drought Stress and Calcium Supply

Forests ◽  
2018 ◽  
Vol 9 (11) ◽  
pp. 667 ◽  
Author(s):  
Liang Xue ◽  
Huadong Ren ◽  
Wei Long ◽  
Xiuhui Leng ◽  
Jia Wang ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Drought Stress ◽  
Water Deficit ◽  
Water Status ◽  
Karst Region ◽  
Drought Treatment ◽  
High Calcium ◽  
Mda Content ◽  
Ecophysiological Responses ◽  
Cyclobalanopsis Glauca

Water deficit and high calcium (Ca2+) content and are two typical soil characteristics in the Karst region. However, the problem of whether high Ca2+ in Karst calcareous soil could increase drought tolerance in calcicole plants has not been solved. We investigated the ecophysiological responses of Cyclobalanopsis glauca (Thunb.) Oerst. cuttings to short-term drought stress and Ca2+ application. Drought stress (10% PEG-6000) markedly reduced relative water content (RWC) and water potential (WP), and enhanced the levels of reactive oxygen species (ROS) production (H2O2 and O2•−) and malondialdehyde (MDA) content in C. glauca leaves. Under drought treatment, exogenous Ca2+ application (20 mM CaCl2) markedly increased the RWC and WP, and reduced the H2O2, O2•−, and MDA content. Furthermore, water deficit induced a significant increase in the activities of antioxidant enzymes such as peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR), and glutathione peroxidase (GPX), and increased the accumulation of osmoregulation substances. External Ca2+ alleviated drought-induced oxidative stress and osmotic stress with further increased activities of antioxidant enzymes, and enhanced the accumulation of osmoregulation substances. In addition, exogenous Ca2+ treatment alleviated the reduction of the photosynthesis rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), and chlorophyll content (SPAD), and further increased water use efficiency (WUE) under drought stress. This study confirms that exogenouos Ca2+ application induces improvements in the water status, osmotic adjustment, antioxidant defense, and photosynthesis efficiency of C. glauca under drought stress.

Seed Germination and Physiological Characteristics of Amaranthus mangostanus L. under Drought Stress

2011 ◽  
Vol 183-185 ◽  
pp. 1071-1074
Author(s):  
Yong Dong Sun ◽  
Xiao Hua Du ◽  
Wen Jie Zhang ◽  
Li Sun ◽  
Ran Li
Keyword(s):  
Drought Stress ◽  
Seed Germination ◽  
Germination Rate ◽  
Proline Content ◽  
Shoot Length ◽  
Physiological Characteristics ◽  
Sod Activity ◽  
Peg 6000 ◽  
Mda Content ◽  
Effects Of Drought

Effects of drought stress on the seed germination and physiological characteristics of amaranth were investigated. The results were as follows: the germination rate and germination potential of amaranth decreased with the increasing of PEG-6000 concentrations. Meanwhile, the root length, shoot length and peroxidase (POD) activity were significantly increased at lower PEG-6000 concentrations, but then decreased with the increasing of PEG-6000. Malondialdehyde (MDA) content, proline content and superoxide dismutase (SOD) activity were all significantly increased under drought stress, and reached the top at 20% PEG-6000. These findings indicated that amaranth tolerates drought stress through increasing the activities of SOD and POD and accumulating proline content.

scholarly journals Influence of Storage Temperature and Duration of Tomato Leaf Samples on Proline Content

2017 ◽  
Vol 13 (6) ◽  
pp. 116
Author(s):  
Mohammad H. Brake ◽  
Hassan R. Hamasha ◽  
Hussein M. Migdadi ◽  
Ashraf O. Khashroum ◽  
Moath Al-Gharaibeh ◽  
...  
Keyword(s):  
Drought Stress ◽  
Agricultural Development ◽  
Storage Temperature ◽  
Field Method ◽  
Economic Benefits ◽  
Proline Content ◽  
Severe Drought ◽  
Agricultural Crop ◽  
Free Proline ◽  
The Impact

In arid and semi-arid countries such as Jordan, shortage in water sources might affect agricultural development and reduces the effectiveness of economic benefits of most crops planted in such areas. Tomato is an important agricultural crop and faces severe drought stress due to climate changes, therefore, measurement of proline accumulation in plant tissues is used as an indicator for drought stress tolerance. This research was conducted at Jarash University Campus in northern Jordan. A field experiment was carried out to investigate the impact of different storage temperature (+4ºC, - 20ºC and -80ºC) and different storage durations (0, 3, 6 and 11 weeks) on proline content in five different Jordanian tomato landraces. Results indicated that the average free proline content for samples tested directly after leaves collection was 7.1 µmol/g. Proline content in leaves stored at +4 ºC for 3, 6, and 11 weeks was 4.8, 1.8, and 1.1µmol/g, respectively, while for -20ºC was 11.8, 7.9, and 9.5 µmol/g for samples stored for 3, 6, 11 weeks respectively. In contrast the highest values for these parameters were obtained from samples stored at -80ºC, the average measured values of free proline content were 9.5, 7.8, and 12.9 µmol/g at 3, 6, and 11 weeks of storage, respectively. Based on the results obtained by this research, it is recommended to measure proline content directly after leaves collection. However, for large number of samples, keeping the samples at -20ºC not longer than six weeks could be a solution. Finally, we highly recommend the development of in-field method for measurement of free proline content.

scholarly journals Enhancement of proline content and antioxidant enzyme activities induced by drought stress in maize (Zea mays L.) by application of compost

2020 ◽  
Vol 13 (7) ◽  
pp. 2978-2990
Author(s):  
Atalaèsso Bokobana ◽  
Outendé Toundou ◽  
Komi Odah ◽  
Koffi S.S. Dossou ◽  
Koffi Tozo
Keyword(s):  
Zea Mays ◽  
Antioxidant Enzymes ◽  
Drought Stress ◽  
Water Deficit ◽  
Sandy Soil ◽  
Zea Mays L ◽  
Proline Content ◽  
Sub Saharan Africa ◽  
Antioxidant Enzyme Activities ◽  
Sub Saharan

In sub-Saharan Africa, soil degradation and recurrent droughts are major obstacles to a sustainable agriculture. This study aimed at investigating the effect of compost addition to soil on proline content and activities of the antioxidant enzymes [catalase (CAT), ascorbate peroxidase (APX)] in maize plants, under drought stress conditions. The test was carried out in 20L plastic pots containing either sandy soil or sandy soil with the fertilizer, under natural conditions. The water deficit was induced at male blooming and milky grain stages. Plant irrigation was done by successive weighing of the pots during which the control is reduced to the same weight corresponding to 70% of the useful water reserve (UWR), while the stressed treatment maintains the water content at 30% of the UWR for 10 days. At the end of the stress period, the proline content, the CAT and APX activities in the leaves were determined through a spectrophotometry. The results show an important accumulation of proline and increase in enzymatic activity induced by water deficit in plants grown on compost (p = 0.00000 at p < 0.05). This study provides evidence for a beneficial effect of compost application in enhancing drought tolerance of maize.Keywords: Drought stress; compost; proline; antioxidant enzymes; Zea mays L.

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