scholarly journals The NAC Protein from Tamarix hispida, ThNAC7, Confers Salt and Osmotic Stress Tolerance by Increasing Reactive Oxygen Species Scavenging Capability

Plants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 221 ◽  
Author(s):  
Zihang He ◽  
Ziyi Li ◽  
Huijun Lu ◽  
Lin Huo ◽  
Zhibo Wang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Abiotic Stress ◽  
Osmotic Stress ◽  
Stress Tolerance ◽  
Oxygen Species ◽  
Tamarix Hispida ◽  
Ros Scavenging ◽  
Transcription Level ◽  
Osmotic Stress Tolerance ◽  
Salt And Osmotic Stress

Plant specific NAC (NAM, ATAF1/2 and CUC2) transcription factors (TFs) play important roles in response to abiotic stress. In this study, we identified and characterized a NAC protein, ThNAC7, from Tamarix hispida. ThNAC7 is a nuclear localized protein and has transcriptional activation activity. ThNAC7 expression was markedly induced by salt and osmotic stresses. Transiently transformed T. hispida seedlings overexpressing ThNAC7 (OE) or with RNA interference (RNAi) silenced ThNAC7 were generated to investigate abiotic stress tolerance via the gain- and loss- of function. Overexpressing ThNAC7 showed an increased reactive oxygen species (ROS) scavenging capabilities and proline content, which was accomplished by enhancing the activities of superoxide dismutase (SOD) and peroxidase (POD) in transiently transformed T. hispida and stably transformed Arabidopsis plants. Additionally, ThNAC7 activated these physiological changes by regulating the transcription level of P5CS, SOD and POD genes. RNA-sequencing (RNA-seq) comparison between wild-type and ThNAC7-transformed Arabidopsis showed that more than 40 known salt tolerance genes might regulated by ThNAC7, including stress tolerance-related genes and TF genes. The results indicated that ThNAC7 induces the transcription level of genes associated with stress tolerance to enhance salt and osmotic stress tolerance via an increase in osmotic potential and enhanced ROS scavenging.

Tamarix hispida aquaporin ThPIP2;5 confers salt and osmotic stress tolerance to transgenic Tamarix and Arabidopsis

2018 ◽  
Vol 152 ◽  
pp. 158-166 ◽  
Author(s):  
Liuqiang Wang ◽  
Chunrui Zhang ◽  
Yanmin Wang ◽  
Yucheng Wang ◽  
Chuanping Yang ◽  
...  
Keyword(s):  
Osmotic Stress ◽  
Stress Tolerance ◽  
Tamarix Hispida ◽  
Osmotic Stress Tolerance ◽  
Salt And Osmotic Stress

scholarly journals CAND2/PMTR1 Is Required for Melatonin-Conferred Osmotic Stress Tolerance in Arabidopsis

2021 ◽  
Vol 22 (8) ◽  
pp. 4014
Author(s):  
Lin-Feng Wang ◽  
Ting-Ting Li ◽  
Yu Zhang ◽  
Jia-Xing Guo ◽  
Kai-Kai Lu ◽  
...  
Keyword(s):  
Osmotic Stress ◽  
Stress Tolerance ◽  
Wild Type Plant ◽  
Wild Type ◽  
Melatonin Receptor ◽  
Ros Scavenging ◽  
Exogenous Melatonin ◽  
Osmotic Stress Tolerance ◽  
Osmotic Stress Response ◽  
In The Wild

Osmotic stress severely inhibits plant growth and development, causing huge loss of crop quality and quantity worldwide. Melatonin is an important signaling molecule that generally confers plant increased tolerance to various environmental stresses, however, whether and how melatonin participates in plant osmotic stress response remain elusive. Here, we report that melatonin enhances plant osmotic stress tolerance through increasing ROS-scavenging ability, and melatonin receptor CAND2 plays a key role in melatonin-mediated plant response to osmotic stress. Upon osmotic stress treatment, the expression of melatonin biosynthetic genes including SNAT1, COMT1, and ASMT1 and the accumulation of melatonin are increased in the wild-type plants. The snat1 mutant is defective in osmotic stress-induced melatonin accumulation and thus sensitive to osmotic stress, while exogenous melatonin enhances the tolerance of the wild-type plant and rescues the sensitivity of the snat1 mutant to osmotic stress by upregulating the expression and activity of catalase and superoxide dismutase to repress H2O2 accumulation. Further study showed that the melatonin receptor mutant cand2 exhibits reduced osmotic stress tolerance with increased ROS accumulation, but exogenous melatonin cannot revert its osmotic stress phenotype. Together, our study reveals that CADN2 functions necessarily in melatonin-conferred osmotic stress tolerance by activating ROS-scavenging ability in Arabidopsis.

scholarly journals The grapevine VvWRKY2 gene enhances salt and osmotic stress tolerance in transgenic Nicotiana tabacum

3 Biotech ◽  
2018 ◽  
Vol 8 (6) ◽  
Author(s):  
Rim Mzid ◽  
Walid Zorrig ◽  
Rayda Ben Ayed ◽  
Karim Ben Hamed ◽  
Mariem Ayadi ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Osmotic Stress ◽  
Stress Tolerance ◽  
Osmotic Stress Tolerance ◽  
Salt And Osmotic Stress ◽  
Transgenic Nicotiana Tabacum
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