scholarly journals Differences among the Anthocyanin Accumulation Patterns and Related Gene Expression Levels in Red Pears

Plants ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 100 ◽  
Author(s):  
Meng Wu ◽  
Jianlong Liu ◽  
Linyan Song ◽  
Xieyu Li ◽  
Liu Cong ◽  
...  
Keyword(s):  
Principal Component ◽  
Hierarchical Cluster ◽  
Anthocyanin Synthesis ◽  
Anthocyanin Accumulation ◽  
Related Gene ◽  
Accumulation Pattern ◽  
Expression Levels ◽  
Coloration Patterns ◽  
Hierarchical Cluster Analyses ◽  
Gene Expression Levels

Differences in coloration exist among red pear cultivars. Here, we selected six red pear cultivars with different genetic backgrounds to elucidate the characteristics of fruit pigmentation. We detected anthocyanin contents and the expression levels of anthocyanin synthesis-related genes in these cultivars at different stages of fruit development. The anthocyanin contents of all six cultivars showed a rise–drop tendency. Principal component and hierarchical cluster analyses were used to distinguish the types of cultivars and the genes crucial to each anthocyanin accumulation pattern. The six cultivars were divided into three groups. Red Zaosu were clustered into one group, Red Sichou and Starkrimson into another group, and Palacer, Red Bartlett, and 5 Hao clustered into a third group. The expression levels of F3H, UFGT2, MYB10, and bHLH3 were similar among the differential coloration patterns of the six cultivars, suggesting a critical and coordinated mechanism for anthocyanin synthesis. Anthocyanin transporters (GST) and light-responsive genes, such as COP1, PIF3.1, and PIF3.2 played limited roles in the regulation of anthocyanin accumulation. This study provides novel insights into the regulation of anthocyanins synthesis and accumulation in red pears.

scholarly journals Clusters of Circulating let-7 Family Tumor Suppressors Are Associated with Clinical Characteristics of Chronic Hepatitis C

2020 ◽  
Vol 21 (14) ◽  
pp. 4945
Author(s):  
Yi-Shan Tsai ◽  
Ming-Lun Yeh ◽  
Pei-Chien Tsai ◽  
Ching-I Huang ◽  
Chung-Feng Huang ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis C ◽  
Chronic Hepatitis C ◽  
Family Members ◽  
Circulatory System ◽  
Principal Component ◽  
Hierarchical Cluster ◽  
Hcv Infection ◽  
Full Spectrum ◽  
Expression Levels

Hepatitis C virus (HCV) infections can cause permanent liver-related diseases, including hepatocellular carcinoma (HCC). Low mortality and incidence of HCC have been observed in patients with chronic hepatitis C undergoing direct-acting antiviral therapy. Tumor suppressive let-7 family members are down-regulated in HCC. The present study, therefore, aimed to investigate whether expression levels for the full spectrum of let-7 family members (let-7a, 7b, 7c, 7d, 7e, 7f, 7g, 7i, and miR-98) in the circulatory system are useful as surveillance biomarkers for liver-related diseases to monitor treatment efficacy during HCV infection. To this end, we measured the levels of mature circulating let-7 family members using quantitative reverse transcription-PCR in 236 patients with HCV infection, and 147 age- and sex-matched controls. Using hierarchical cluster analysis and principal component analysis, three clusters were obtained after measuring expression levels of let-7 family members in the patients and controls. Cluster 1 included let-7a/d/e/g, Cluster 2 comprised let-7b and let-7i, and Cluster 3 comprised let-7c/f/miR-98. Let-7b/c/g represented the three clusters and showed the best survival response to liver cancer when analyzed with respect to patient data. Therefore, considering the circulating levels of let7 b/c/g as representatives of the let-7 family may facilitate effective monitoring of liver-related disease.

5-fluorouracil related gene expression levels in primary colorectal cancer and corresponding liver metastases

2005 ◽  
Vol 23 (16_suppl) ◽  
pp. 3568-3568 ◽  
Author(s):  
H. Kuramochi ◽  
K. Hayashi ◽  
K. Uchida ◽  
D. Shimizu ◽  
D. Vallboehmer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Liver Metastases ◽  
Related Gene ◽  
Primary Colorectal Cancer ◽  
Expression Levels ◽  
Gene Expression Levels

scholarly journals 1H NMR-Based Chemometrics to Gain Insights Into the Bran of Radiation-Induced Colored Wheat Mutant

2022 ◽  
Vol 8 ◽  
Author(s):  
Yun-Seo Kil ◽  
Ah-Reum Han ◽  
Min-Jeong Hong ◽  
Jin-Baek Kim ◽  
Pil-Hoon Park ◽  
...  
Keyword(s):  
Antioxidant Properties ◽  
Principal Component ◽  
Hierarchical Cluster ◽  
Primary Metabolites ◽  
H Nmr ◽  
Induced Mutants ◽  
Radiation Induced ◽  
Mutant Lines ◽  
Hierarchical Cluster Analyses ◽  
High Polarity

Recently, wheat has attracted attention as a functional food, rather than a simple dietary energy source. Accordingly, whole-grain intake increases with an understanding of bioactive phytochemicals in bran. The development of colored wheat has drawn more attention to the value of bran owing to its nutritional quality, as well as the antioxidant properties of the colorant. The present 1H NMR-based chemometric study evaluated the compositional improvement of radiation-induced mutants in purple wheat by focusing on the predominant metabolites with high polarity. A total of 33 metabolites, including three choline derivatives, three sugar alcohols, four sugars, 13 amino acids, eight organic acids, and two nucleosides, were identified throughout the 1H NMR spectra, and quantification data were obtained for the identified metabolites via peak shape-based quantification. Principal component and hierarchical cluster analyses were conducted for performing multivariate analyses. The colored original wheat was found to exhibit improvements compared to yellow wheat in terms of the contents of primary metabolites, thus highlighting the importance of conducting investigations of polar metabolites. The chemometrics studies further revealed mutant lines with a compositional enhancement for metabolites, including lysine, proline, acetate, and glycerol.

scholarly journals 5-fluorouracil-related gene expression levels in primary colorectal cancer and corresponding liver metastasis

2006 ◽  
Vol 119 (3) ◽  
pp. 522-526 ◽  
Author(s):  
Hidekazu Kuramochi ◽  
Kazuhiko Hayashi ◽  
Kazumi Uchida ◽  
Satoru Miyakura ◽  
Daisuke Shimizu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Liver Metastasis ◽  
Related Gene ◽  
Primary Colorectal Cancer ◽  
Expression Levels ◽  
Gene Expression Levels

Principal component and hierarchical cluster analyses for classification and categorization of sesame germplasm

2015 ◽  
Vol 16 (2) ◽  
pp. 345
Author(s):  
Amit Singh ◽  
Ram Avtar ◽  
Nisha Kumari ◽  
O. Sangwan ◽  
R. K. Sheoran
Keyword(s):  
Principal Component ◽  
Hierarchical Cluster ◽  
Cluster Analyses ◽  
Hierarchical Cluster Analyses
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