scholarly journals Karyological Analysis and DNA Barcoding of Pompia Citron: A First Step toward the Identification of Its Relatives

Plants ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 83 ◽  
Author(s):  
Grazia Viglietti ◽  
Giulio Galla ◽  
Andrea Porceddu ◽  
Gianni Barcaccia ◽  
Franck Curk ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Dna Content ◽  
Nuclear Dna ◽  
Genetic Relationships ◽  
Citrus Fruit ◽  
Plastid Dna ◽  
Molecular Data ◽  
Dna Polymorphisms ◽  
Citrus Species ◽  
Chloroplast Markers

Pompia is a citrus fruit endemic of Sardinia, Italy, with an essential oil profile showing outstanding anti-inflammatory and anti-microbic properties. Despite its remarkable pharmaceutical potential, little taxonomic and genetic information is available for this species. We applied flow cytometry and classical cytogenetic techniques to assess the DNA content and to reconstruct the karyotype of several Pompia accessions. Molecular data from plastid DNA barcoding and nuclear DNA sequencing were used to study the genetic distance between Pompia and other citrus species. Flow cytometric estimates of DNA content and somatic chromosome counts suggest that Pompia is a regular diploid Citrus species. DNA polymorphisms of nuclear and chloroplast markers allowed us to investigate the genetic relationships between Pompia accessions and other Citrus species. Based on DNA polymorphism data we propose that Pompia is a very recent interspecific hybrid generated by a cross between C. aurantium (as seed bearer) and C. medica (as pollen donor). Our findings pave the way for further and more specific investigations of local Pompia germplasm resources that may help the preservation and valorisation of this valuable citrus fruit tree.

scholarly journals The solution to the cytological paradox of isomorphy.

1987 ◽  
Vol 104 (3) ◽  
pp. 739-748 ◽  
Author(s):  
L J Goff ◽  
A W Coleman
Keyword(s):  
Cell Size ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Plastid Dna ◽  
Diploid Cell ◽  
Red Alga ◽  
Haploid Nucleus ◽  
Two Generations ◽  
A Cell

Cells with polyploid nuclei are generally larger than cells of the same organism or species with nonpolyploid nuclei. However, no such change of cell size with ploidy level is observed in those red algae which alternate isomorphic haploid with diploid generations. The results of this investigation reveal the explanation. Nuclear DNA content and other parameters were measured in cells of the filamentous red alga Griffithsia pacifica. Nuclei of the diploid generation contain twice the DNA content of those of the haploid generation. However, all cells except newly formed reproductive cells are multinucleate. The nuclei are arranged in a nearly perfect hexagonal array just beneath the cell surface. When homologous cells of the two generations are compared, although the cell size is nearly identical, each nucleus of the diploid cell is surrounded by a region of cytoplasm (a "domain") nearly twice that surrounding a haploid nucleus. Cytoplasmic domains associated with a diploid nucleus contain twice the number of plastids, and consequently twice the amount of plastid DNA, than is associated with the domain of a haploid nucleus. Thus, doubling of ploidy is reflected in doubling of the size and organelle content of the domain associated with each nucleus. However, cell size does not differ between homologous cells of the two generations, because total nuclear DNA (sum of the DNA in all nuclei in a cell) per cell does not differ. This is the solution to the cytological paradox of isomorphy.

Genome size differences in Hyalella cryptic species

Genome ◽  
2012 ◽  
Vol 55 (2) ◽  
pp. 134-139 ◽  
Author(s):  
Roland Vergilino ◽  
Kaven Dionne ◽  
Christian Nozais ◽  
France Dufresne ◽  
Claude Belzile
Keyword(s):  
Flow Cytometry ◽  
Cryptic Species ◽  
Dna Barcoding ◽  
Genome Size ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Complex Species ◽  
Numerous Species ◽  
Freshwater Habitats

The Hyalella azteca (Saussure) complex includes numerous amphipod cryptic species in freshwater habitats in America as revealed by DNA barcoding surveys. Two ecomorphs (small and large) have evolved numerous times in this complex. Few phenotypic criteria have been found to differentiate between the numerous species of this complex. The present study aims to explore genome size differences between some species of the H. azteca complex co-occurring in a Canadian boreal lake using flow cytometry. Nuclear DNA content was estimated for 50 individuals belonging to six COI haplotypes corresponding to four provisional species of the H. azteca complex. Species from the large ecomorph had C-values significantly larger than species from the small ecomorph, whereas slight differences were found among species of the small ecomorph. These differences in genome sizes might be linked to ecological and physiological differences among species of the H. azteca complex.

scholarly journals Genetic relationships in the genus Gentiana based on chloroplast DNA sequence data and nuclear DNA content

2009 ◽  
Vol 59 (2) ◽  
pp. 119-127 ◽  
Author(s):  
Kei-ichiro Mishiba ◽  
Kyoko Yamane ◽  
Takashi Nakatsuka ◽  
Yuki Nakano ◽  
Saburo Yamamura ◽  
...  
Keyword(s):  
Chloroplast Dna ◽  
Dna Sequence ◽  
Dna Content ◽  
Nuclear Dna ◽  
Sequence Data ◽  
Genetic Relationships ◽  
Nuclear Dna Content ◽  
Dna Sequence Data

scholarly journals Genetic diversity and phylogeography of the rare riparian moss Dichelyma capillaceum (With.) Myr. inferred from trnL-F plastid DNA sequences

2009 ◽  
Vol 61 (1) ◽  
pp. 85-91 ◽  
Author(s):  
M. Sabovljevic ◽  
J.P. Frahm
Keyword(s):  
Genetic Diversity ◽  
Dna Sequences ◽  
North American ◽  
Genetic Relationships ◽  
Plastid Dna ◽  
Molecular Data ◽  
Genetic Distances ◽  
German Population ◽  
F Region

The genetic relationships of Dichelyma capillaceum (With.) Myr. are studied from chloroplast sequences of the trnL-F region. On the basis of the molecular data obtained, the German population can be considered to be derived from Scandinavian(Swedish) rather than North American populations. To judge from the genetic distances between the Swedish and German populations, the separation must have occurred along time ago.

NUCLEAR DNA CONTENT AND GENETIC RELATIONSHIPS BASED ON AFLP DATA IN HELLEBORUS

2012 ◽  
pp. 157-162
Author(s):  
J. Meiners ◽  
T. Debener ◽  
G. Schweizer ◽  
T. Winkelmann
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Genetic Relationships ◽  
Nuclear Dna Content ◽  
Aflp Data

Prognostic value of nuclear DNA content in papillary and follicular thyroid cancer

1988 ◽  
Vol 12 (4) ◽  
pp. 503-507 ◽  
Author(s):  
Jaap F. Hamming ◽  
Lodewijk J. D. M. Schelfhout ◽  
Cees J. Cornelisse ◽  
Cornelis J. H. van de Velde ◽  
Bernard M. Goslings ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Dna Content ◽  
Prognostic Value ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Follicular Thyroid Cancer

scholarly journals The Role of Mitochondria in Carcinogenesis

2021 ◽  
Vol 22 (10) ◽  
pp. 5100
Author(s):  
Paulina Kozakiewicz ◽  
Ludmiła Grzybowska-Szatkowska ◽  
Marzanna Ciesielka ◽  
Jolanta Rzymowska
Keyword(s):  
Prostate Cancer ◽  
Mitochondrial Dna ◽  
Nuclear Dna ◽  
Dna Polymorphisms ◽  
Gene Encoding ◽  
Dna Mutations ◽  
Nadh Ubiquinone Oxidoreductase ◽  
Gene Coi ◽  
The Impact

The mitochondria are essential for normal cell functioning. Changes in mitochondrial DNA (mtDNA) may affect the occurrence of some chronic diseases and cancer. This process is complex and not entirely understood. The assignment to a particular mitochondrial haplogroup may be a factor that either contributes to cancer development or reduces its likelihood. Mutations in mtDNA occurring via an increase in reactive oxygen species may favour the occurrence of further changes both in mitochondrial and nuclear DNA. Mitochondrial DNA mutations in postmitotic cells are not inherited, but may play a role both in initiation and progression of cancer. One of the first discovered polymorphisms associated with cancer was in the gene NADH-ubiquinone oxidoreductase chain 3 (mt-ND3) and it was typical of haplogroup N. In prostate cancer, these mutations and polymorphisms involve a gene encoding subunit I of respiratory complex IV cytochrome c oxidase subunit 1 gene (COI). At present, a growing number of studies also address the impact of mtDNA polymorphisms on prognosis in cancer patients. Some of the mitochondrial DNA polymorphisms occur in both chronic disease and cancer, for instance polymorphism G5913A characteristic of prostate cancer and hypertension.

Estimation of nuclear DNA content by flow cytometry in fishes of the genus Xiphophorus

1989 ◽  
Vol 94 (3) ◽  
pp. 465-468 ◽  
Author(s):  
Terrence R. Tiersch ◽  
Robert W. Chandler ◽  
Klaus D. Kallman ◽  
Stephen S. Wachtel
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content
Sign in / Sign up

Export Citation Format

Share Document