scholarly journals Isolation and Characterization of Pseudomonas chlororaphis Strain ST9; Rhizomicrobiota and in Planta Studies

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1466
Author(s):  
Iris Bertani ◽  
Elisa Zampieri ◽  
Cristina Bez ◽  
Andrea Volante ◽  
Vittorio Venturi ◽  
...  
Keyword(s):  
Plant Growth ◽  
Crop Yields ◽  
Whole Genome Sequence ◽  
Pseudomonas Chlororaphis ◽  
Plant Tolerance ◽  
In Planta ◽  
Biotic Stresses ◽  
Isolation And Characterization ◽  
Plant Genes

The development of biotechnologies based on beneficial microorganisms for improving soil fertility and crop yields could help to address many current agriculture challenges, such as food security, climate change, pest control, soil depletion while decreasing the use of chemical fertilizers and pesticides. Plant growth-promoting (PGP) microbes can be used as probiotics in order to increase plant tolerance/resistance to abiotic/biotic stresses and in this context strains belonging to the Pseudomonas chlororaphis group have shown to have potential as PGP candidates. In this study a new P. chlororaphis isolate is reported and tested for (i) in vitro PGP features, (ii) whole-genome sequence analysis, and (iii) its effects on the rhizosphere microbiota composition, plant growth, and different plant genes expression levels in greenhouse experiments. Results showed that P. chlororaphis ST9 is an efficient rice root colonizer which integrates into the plant resident-microbiota and affects the expression of several plant genes. The potential use of this P. chlororaphis strain as a plant probiotic is discussed.

scholarly journals Isolation and characterization of Pseudomonas chlororaphis strain ST9 and its potential as a bioinoculant for agriculture

2020 ◽  
Author(s):  
Iris Bertani ◽  
Elisa Zampieri ◽  
Cristina Bez ◽  
Andrea Volante ◽  
Vittorio Venturi ◽  
...  
Keyword(s):  
Plant Growth ◽  
Crop Yields ◽  
Whole Genome Sequence ◽  
Pseudomonas Chlororaphis ◽  
Plant Tolerance ◽  
Biotic Stresses ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Plant Genes

AbstractThe development of biotechnologies based on beneficial microorganisms for improving soil fertility and crop yields could help addressing many current agriculture challenges, such as food security, climate change, pests control, soil depletion while decreasing the use of chemical fertilizers and pesticides. Plant Growth Promoting (PGP) microbes can be used as probiotics in order to increase plant tolerance/resistance to abiotic/biotic stresses and in this context strains belonging to the Pseudomonas chlororaphis group have shown to have potential as PGP candidates. In this work a new P. chlororaphis isolate is reported and tested for (i) in vitro PGP features, (ii) whole genome sequence analysis, and (iii) its effects on root microbiome, plant growth and on the expression of different plant genes in greenhouse experiments. The potential use of this P. chlororaphis strain as a plant probiotic is discussed.

scholarly journals From Strain Characterization to Field Authorization: Highlights on Bacillus velezensis Strain B25 Beneficial Properties for Plants and Its Activities on Phytopathogenic Fungi

2021 ◽  
Vol 9 (9) ◽  
pp. 1924
Author(s):  
Pierre Joly ◽  
Alexandra Calteau ◽  
Aurélie Wauquier ◽  
Rémi Dumas ◽  
Mylène Beuvin ◽  
...  
Keyword(s):  
Plant Growth ◽  
Crop Yields ◽  
Antibiotic Resistance Genes ◽  
Pathogenic Fungi ◽  
Phytopathogenic Fungi ◽  
In Planta ◽  
Bacillus Velezensis ◽  
Plant Growth Promoting ◽  
Growth Promoting

Agriculture is in need of alternative products to conventional phytopharmaceutical treatments from chemical industry. One solution is the use of natural microorganisms with beneficial properties to ensure crop yields and plant health. In the present study, we focused our analyses on a bacterium referred as strain B25 and belonging to the species Bacillus velezensis (synonym B. amyloliquefaciens subsp. plantarum or B. methylotrophicus), a promising plant growth promoting rhizobacterium (PGPR) and an inhibitor of pathogenic fungi inducing crops diseases. B25 strain activities were investigated. Its genes are well preserved, with their majority being common with other Bacillus spp. strains and responsible for the biosynthesis of secondary metabolites known to be involved in biocontrol and plant growth-promoting activities. No antibiotic resistance genes were found in the B25 strain plasmid. In vitro and in planta tests were conducted to confirm these PGPR and biocontrol properties, showing its efficiency against 13 different pathogenic fungi through antibiosis mechanism. B25 strain also showed good capacities to quickly colonize its environment, to solubilize phosphorus and to produce siderophores and little amounts of auxin-type phytohormones (around 13,051 µg/mL after 32 h). All these findings combined to the fact that B25 demonstrated good properties for industrialization of the production and an environmental-friendly profile, led to its commercialization under market authorization since 2018 in several biostimulant preparations and opened its potential use as a biocontrol agent.

scholarly journals Isolation and Characterization of Salt Tolerant Endophytic and Rhizospheric Plant Growth-Promoting Bacteria (PGPB) Associated with the Halophyte Plant (Sesuvium Verrucosum) Grown in KSA

2015 ◽  
Vol 3 (3) ◽  
pp. 552-560 ◽  
Author(s):  
Mohamed A.M. El-Awady ◽  
Mohamed M. Hassan ◽  
Yassin M. Al-Sodany
Keyword(s):  
Nitrogen Fixation ◽  
Acetic Acid ◽  
Plant Growth ◽  
Growth Promotion ◽  
Exponential Growth Phase ◽  
Plant Growth Promoting Bacteria ◽  
Salt Tolerant ◽  
Isolation And Characterization ◽  
Growth Promoting

This study was designed to isolate and characterize endophytic and rhizospheric bacteria associated with the halophyte plant Sesuvium verrucosum, grown under extreme salinity soil in Jeddah, Saudi Arabia. The plant growth promotion activities of isolated bacterial were evaluated in vitro. A total of 19 salt tolerant endophytic and rhizospheric bacterial isolates were obtained and grouped into six according to genetic similarity based on RAPD data. These six isolates were identified by amplification and partial sequences of 16S rDNA as Enterobacter cancerogenus,Vibrio cholerae, Bacillus subtilis, Escherichia coli and two Enterobacter sp. Isolates were then grown until exponential growth phase to evaluate the atmospheric nitrogen fixation, phosphate solubilization, and production of phytohormones such as indole-3-acetic acid, as well as 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity. While, All of the six strains were negative for ACC deaminaseactivity, two isolates showed Nitrogen fixation activity, three isolates produce the plant hormone (Indole acetic acid) and two isolates have the activity of solubiliztion of organic phosphate. Among the six isolates, the isolate (R3) from the soil around the roots is able to perform the three previous growth promoting possibilities together and it is ideal for use in promoting the growth of plants under the high salinity conditions. This isolate is candidate to prepare a friendly biofertelizer that can be used for the improvement of the crops performance under salinity conditions.Int J Appl Sci Biotechnol, Vol 3(3): 552-560

scholarly journals Analysis of expressed sequence tags derived from the endophytic fungus Neotyphodium lolii grown in vitro and in association with its host plant perennial ryegrass

2007 ◽  
Vol 13 ◽  
pp. 501-504
Author(s):  
R. Johnson ◽  
A. Khan ◽  
C. Voisey ◽  
S. Bassett ◽  
C. Gaborit ◽  
...  
Keyword(s):  
Expressed Sequence Tags ◽  
Virulence Genes ◽  
In Planta ◽  
Suppression Subtractive Hybridisation ◽  
The Public ◽  
Neotyphodium Lolii ◽  
Subtractive Hybridisation ◽  
Plant Genes ◽  
Expressed Sequence

As a first step towards a functional genomics approach to gain a greater understanding of this important symbiosis, we have generated, sequenced and analysed two EST libraries from cultures of N. lolii and six in planta subtracted EST libraries enriched for differentially expressed genes. A total of 12871 ESTs were sequenced which, after filtering for quality, clustered into 1066 contigs and 3230 singletons to give a set of 4296 unique sequences or unigenes. BLASTX analysis revealed that 60% of fungal sequences derived from cultures were of unknown function with a sub-set of these corresponding to orphans. For the in planta-derived ESTs, most of the sequences with homologs in the public databases (98%) were of ryegrass origin. Comparisons made against fully sequenced genomes revealed that most fungal ESTs were homologous to genes present in both pathogenic and non-pathogenic ascomycete filamentous fungi, whereas the subtracted libraries comprised mostly plant genes. A range of sequences having significant homology to demonstrated pathogenicity/virulence genes in other fungal pathosystems were also identified, as well as some ESTs with proven roles in endophyte secondary metabolism. Keywords: ESTs, cDNA, Neotyphodium lolii, Lolium perenne, symbiosis, mutualism, suppression subtractive hybridisation

scholarly journals Melatonin and Its Protective Role against Biotic Stress Impacts on Plants

Biomolecules ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 54 ◽  
Author(s):  
Mohamed Moustafa-Farag ◽  
Abdulwareth Almoneafy ◽  
Ahmed Mahmoud ◽  
Amr Elkelish ◽  
Marino B. Arnao ◽  
...  
Keyword(s):  
Plant Resistance ◽  
Biotic Stress ◽  
Growth Regulation ◽  
Protective Role ◽  
Virus Concentration ◽  
Plant Tolerance ◽  
Biotic And Abiotic Stress ◽  
Interaction Models ◽  
Biotic Stresses

Biotic stress causes immense damage to agricultural products worldwide and raises the risk of hunger in many areas. Plants themselves tolerate biotic stresses via several pathways, including pathogen-associated molecular patterns (PAMPs), which trigger immunity and plant resistance (R) proteins. On the other hand, humans use several non-ecofriendly methods to control biotic stresses, such as chemical applications. Compared with chemical control, melatonin is an ecofriendly compound that is an economical alternative strategy which can be used to protect animals and plants from attacks via pathogens. In plants, the bactericidal capacity of melatonin was verified against Mycobacterium tuberculosis, as well as multidrug-resistant Gram-negative and -positive bacteria under in vitro conditions. Regarding plant–bacteria interaction, melatonin has presented effective antibacterial activities against phytobacterial pathogens. In plant–fungi interaction models, melatonin was found to play a key role in plant resistance to Botrytis cinerea, to increase fungicide susceptibility, and to reduce the stress tolerance of Phytophthora infestans. In plant–virus interaction models, melatonin not only efficiently eradicated apple stem grooving virus (ASGV) from apple shoots in vitro (making it useful for the production of virus-free plants) but also reduced tobacco mosaic virus (TMV) viral RNA and virus concentration in infected Nicotiana glutinosa and Solanum lycopersicum seedlings. Indeed, melatonin has unique advantages in plant growth regulation and increasing plant resistance effectiveness against different forms of biotic and abiotic stress. Although considerable work has been done regarding the role of melatonin in plant tolerance to abiotic stresses, its role in biotic stress remains unclear and requires clarification. In our review, we summarize the work that has been accomplished so far; highlight melatonin’s function in plant tolerance to pathogens such as bacteria, viruses, and fungi; and determine the direction required for future studies on this topic.

scholarly journals Toward the molecular understanding of the action mechanism of Ascophyllum nodosum extracts on plants

2019 ◽  
Vol 32 (1) ◽  
pp. 573-597 ◽  
Author(s):  
Jonas De Saeger ◽  
Stan Van Praet ◽  
Danny Vereecke ◽  
Jihae Park ◽  
Silke Jacques ◽  
...  
Keyword(s):  
Plant Growth ◽  
Ascophyllum Nodosum ◽  
Molecular Data ◽  
Potential Contribution ◽  
In Planta ◽  
Biotic Stresses ◽  
Ethnographic Studies ◽  
Hormonal Content ◽  
Plant Growth Promoting ◽  
Promote Plant Growth

Abstract The importance of biostimulants, defined as plant growth-promoting agents that differ notably from fertilizers, is increasing steadily because of their potential contribution to a worldwide strategy for securing food production without burdening the environment. Based on folkloric evidence and ethnographic studies, seaweeds have been useful for diverse human activities through time, including medicine and agriculture. Currently, seaweed extracts, especially those derived from the common brown alga Ascophyllum nodosum, represent an interesting category of biostimulants. Although A. nodosum extracts (abbreviated ANEs) are readily used because of their capacity to improve plant growth and to mitigate abiotic and biotic stresses, fundamental insights into how these positive responses are accomplished are still fragmentary. Generally, the effects of ANEs on plants have been attributed to their hormonal content, their micronutrient value, and/or the presence of alga-specific polysaccharides, betaines, polyamines, and phenolic compounds that would, alone or in concert, bring about the observed phenotypic effects. However, only a few of these hypotheses have been validated at the molecular level. Transcriptomics and metabolomics are now emerging as tools to dissect the action mechanisms exerted by ANEs. Here, we provide an overview of the available in planta molecular data that shed light on the pathways modulated by ANEs that promote plant growth and render plants more resilient to diverse stresses, paving the way toward the elucidation of the modus operandi of these extracts.

scholarly journals Engineering the phototropin photocycle improves photoreceptor performance and plant biomass production

2019 ◽  
Vol 116 (25) ◽  
pp. 12550-12557 ◽  
Author(s):  
Jaynee E. Hart ◽  
Stuart Sullivan ◽  
Paweł Hermanowicz ◽  
Jan Petersen ◽  
L. Aranzazú Diaz-Ramos ◽  
...  
Keyword(s):  
Plant Growth ◽  
Biomass Production ◽  
Plant Biomass ◽  
Targeted Mutagenesis ◽  
Light Conditions ◽  
In Planta ◽  
Low Light ◽  
Light Regimes ◽  
Plant Biomass Production

The ability to enhance photosynthetic capacity remains a recognized bottleneck to improving plant productivity. Phototropin blue light receptors (phot1 and phot2) optimize photosynthetic efficiency in Arabidopsis thaliana by coordinating multiple light-capturing processes. In this study, we explore the potential of using protein engineering to improve photoreceptor performance and thereby plant growth. We demonstrate that targeted mutagenesis can decrease or increase the photocycle lifetime of Arabidopsis phototropins in vitro and show that these variants can be used to reduce or extend the duration of photoreceptor activation in planta. Our findings show that slowing the phototropin photocycle enhanced several light-capturing responses, while accelerating it reduced phototropin’s sensitivity for chloroplast accumulation movement. Moreover, plants engineered to have a slow-photocycling variant of phot1 or phot2 displayed increased biomass production under low-light conditions as a consequence of their improved sensitivity. Together, these findings demonstrate the feasibility of engineering photoreceptors to manipulate plant growth and offer additional opportunities to enhance photosynthetic competence, particularly under suboptimal light regimes.

Isolation and characterization of plant growth-promoting rhizobacteria from Opuntia ficus-indica and their effect on Wheat growth

2020 ◽  
Vol 9 (6) ◽  
pp. 255-265
Author(s):  
Draou N ◽  
Selami N ◽  
Karkachi N ◽  
Kacem Mourad ◽  
Kaid Harche M
Keyword(s):  
Plant Growth ◽  
Arid Region ◽  
Antagonistic Effect ◽  
Phytopathogenic Fungus ◽  
Klebsiella Pneumonia ◽  
Bacterial Strains ◽  
Isolation And Characterization ◽  
Semi Arid Region ◽  
Semi Arid

Endophytic bacteria are found inside host plants without causing disease symptoms, and form a range of di erent bene cial rela onships. In this study, four bacterial strains were isolated from roots, cladodes and non gus- tatory fruits of Opun a cus-indicaL. collected from the Algerian semi-arid region. The bacteria were able to grow at temperatures as 45 °C. 16SrRNA sequencing revealed the iden ty of isolates as Pseudomonas pu da(E1)with 98% similarity,P. brassicacearum(E2) and Klebsiella pneumonia (E3,E4)with 99% similarity. The strains were tested in vitro for their biocontrol ac vity against phytopathogenic fungus, and found to have the ability to inhibit the Fusarium oxysporum f. sp. albedinis and Aspergillus niger growth. We noticed that the most important inhibitory effect recorded with P. pu da (69.78±3.93 and 65.15±2.8), and that the feeble antagonistic effect recorded with P. brassicacearum (20.57±6.04 and 8.52 ±2.8) against of F.oxysporum f.sp albdeinis and A.niger. The plant growth promo ng (PGP) proper es were demonstrated through wheat (Triticum aestivum L.) based bioassay under hydroponic condi ons.The inocula on of bacteria showed an increase in density, length of roots and stems, indica ng the PGP abili es of these bacteria. P. pu da showed a poten al for growth of wheat plant.We no ced respectively the length of both stems and roots (17.34 ± 0.54 mm; 11.07 ± 0.67 mm), and we found that fresh and dry weight was (0.23 ± 0.029 g; 0.0375 ±0.003 g). Overall the results showed that isolates characterized were able to improve the growth of plants, and inhibit the propaga on of phytopathogenic fungi. To our knowledge, this is the rst report describing the isola on of bacteria from O. cus-indica(L.) in Algerian semi-arid region.

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