scholarly journals Finding Needles in a Haystack: Using Geo-References to Enhance the Selection and Utilization of Landraces in Breeding for Climate-Resilient Cultivars of Upland Cotton (Gossypium hirsutum L.)

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1300
Author(s):  
Junghyun Shim ◽  
Nonoy B. Bandillo ◽  
Rosalyn B. Angeles-Shim
Keyword(s):  
Allelic Diversity ◽  
Potential Donor ◽  
Breeding Programs ◽  
Germplasm Collections ◽  
Climate Shifts ◽  
Potential Applications ◽  
Genetic Value ◽  
Modern Breeding ◽  
Efficient Selection ◽  
Selection Of

The genetic uniformity of cultivated cotton as a consequence of domestication and modern breeding makes it extremely vulnerable to abiotic challenges brought about by major climate shifts. To sustain productivity amidst worsening agro-environments, future breeding objectives need to seriously consider introducing new genetic variation from diverse resources into the current germplasm base of cotton. Landraces are genetically heterogeneous, population complexes that have been primarily selected for their adaptability to specific localized or regional environments. This makes them an invaluable genetic resource of novel allelic diversity that can be exploited to enhance the resilience of crops to marginal environments. The utilization of cotton landraces in breeding programs are constrained by the phenology of the plant and the lack of phenotypic information that can facilitate efficient selection of potential donor parents for breeding. In this review, the genetic value of cotton landraces and the major challenges in their utilization in breeding are discussed. Two strategies namely Focused Identification of Germplasm Strategy and Environmental Association Analysis that have been developed to effectively screen large germplasm collections for accessions with adaptive traits using geo-reference-based, mathematical modelling are highlighted. The potential applications of both approaches in mining available cotton landrace collections are also presented.

scholarly journals Barcoding of ToLCV Resistant Tomato Germplasm in Bangladesh

2020 ◽  
Vol 30 (1) ◽  
pp. 107-117
Author(s):  
Mahbubah Jannat ◽  
Umma Homaira Eva ◽  
RH Sarker ◽  
Mohammad Nurul Islam
Keyword(s):  
Nuclear Genome ◽  
Intergenic Spacer ◽  
Pcr Analysis ◽  
Correct Identification ◽  
Potential Donor ◽  
Breeding Programs ◽  
Intergenic Spacer Region ◽  
Mitochondrial Region ◽  
Successful Amplification ◽  
Modern Breeding

The current study was carried out to confirm the existence of the ToLCV resistant genes (Ty-1 to Ty-5) in the germplasm using molecular markers and to identify at the genomic level following phylogenetic relationships analysis among some local tomato germplasm using DNA barcoding. Most of the tomato germplasm (Ten out of 14) contain the dominant Ty-genes as revealed by PCR analysis. “Barcoding” of the non-coding plastid trnH-psbA intergenic spacer region, three plastidal regions: rbcL, rpoB, rpoC1, spacer region of nuclear genome ITS and a mitochondrial region matK were employed following PCR and sequence analysis of the germplasm. Among all the barcode genes, rpoB, rbcL, trnH-psbA and ITS were leading candidates for successful amplification and used for the identification of the germplasm as S. lycopersicum in multi-locus identification based on their sequences. Neighbor-Joining phylogenetic tree was constructed in which the germplasm were clustered into five main clades. The current study was successful to establish an efficient barcoding protocol for the correct identification of tomatoes and was capable of establishing elite gene source(s) for biotic stress resistance tomato varieties which would serve as potential donor plants in modern breeding programs. Plant Tissue Cult. & Biotech. 30(1): 107-117, 2020 (June)

scholarly journals Short communication: Identification of cultivated and wild Vaccinium species grown in Portugal

2018 ◽  
Vol 16 (3) ◽  
pp. e07SC01
Author(s):  
Márcia Carvalho ◽  
Manuela Matos ◽  
Valdemar Carnide
Keyword(s):  
Short Communication ◽  
Vaccinium Corymbosum ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Polymorphic Markers ◽  
Wild Populations ◽  
Breeding Programs ◽  
Germplasm Collections ◽  
Simple Sequence ◽  
Selection Of

Vaccinium crops offer a variety of benefits for human health due their high levels of antioxidants. Genetic diversity between two Vaccinium species (sixteen cultivars of Vaccinium corymbosum and three wild populations of Vaccinium myrtillus) were evaluated using Inter Simple Sequence Repeat (ISSR) markers. In V. corymbosum 74 polymorphic markers corresponding to 83.2% of polymorphism were obtained while in V. myrtillus only four polymorphic markers corresponding to 83.2% and 10.6% of polymorphism were observed. The dendrogram obtained showed a clear division into two distinct groups corresponding to the two analyzed species. V. corymbosum group is divided in different sub-clusters based on cultivars pedigree relationships. Twenty-eight specific bands were detected in total; 6 for V. corymbosum and 22 for V. myrtillus. Results allowed the selection of five primers due it potential to detect specific bands in the two species. These markers could be useful for identifying species and cultivars and consequently help in the management of germplasm collections and in breeding programs.

scholarly journals COMPLEMENTARY TOOLS UTILIZED IN THE PEA (Pisum sativum L.) BREEDING PROGRAM AT UNIVERSIDAD NACIONAL DE ROSARIO

2021 ◽  
Vol 32 (Issue 2) ◽  
pp. 24-30
Author(s):  
Guindón M.F. ◽  
F. Cazzola ◽  
C.J. Bermejo ◽  
M.A. Espósito ◽  
I. Gatti ◽  
...  
Keyword(s):  
Molecular Markers ◽  
In Vitro Culture ◽  
Linear Unbiased Prediction ◽  
Germplasm Collections ◽  
Digital Phenotyping ◽  
Rate Of Increase ◽  
Best Linear Unbiased ◽  
Efficient Selection ◽  
Selection Of

Conventional breeding can be complemented by different strategies that increase the efficiency of the methodologies and the current rate of increase in yields in order to meet demand. The use of molecular markers with the aim of developing linkage maps of the species, the use of Blup (Best Linear Unbiased Prediction) for an efficient selection of progenitors to hybridize, the use of in vitro culture to artificially increase the number of F1 plants or the use of digital phenotyping for efficient digital characterization that can be performed during the periodic and routine regeneration of accessions in germplasm collections. Key words: Molecular markers, Blup, in vitro culture, digital phenotyping.

scholarly journals Development and Evaluation of a Portable, Handheld Mechanical Shaker to Study Fruit Detachment in Blueberry

HortScience ◽  
2013 ◽  
Vol 48 (3) ◽  
pp. 394-397 ◽  
Author(s):  
Anish Malladi ◽  
Tripti Vashisth ◽  
Scott NeSmith
Keyword(s):  
Vaccinium Corymbosum ◽  
Fruit Production ◽  
Highbush Blueberry ◽  
Breeding Programs ◽  
Vaccinium Ashei ◽  
Potential Applications ◽  
Southern Highbush ◽  
Time Required ◽  
Mechanical Shaker ◽  
Selection Of

A portable, handheld, mechanical shaking device was developed and its effects on fruit detachment in rabbiteye bluebbery (Vaccinium ashei Reade) and southern highbush blueberry (hybrids of mostly Vaccinium corymbosum L. and Vaccinium darrowi Camp.) were evaluated. The instrument facilitated effective detachment of fruit within a branch, especially in rabbiteye blueberry (greater than 75%). Approximately 3 to 4 seconds of shaking was sufficient to detach the majority of the fruit. Differences in the extent of fruit detachment were observed across the genotypes, especially among southern highbush blueberry genotypes. The majority of fruit detachment in rabbiteye blueberry and most of the southern highbush blueberry genotypes occurred at the point of attachment of the pedicel to the berry, although a significant portion detached with the stem intact, resulting in stemmy fruit. Although only a small proportion of the detached fruit was immature in the rabbiteye blueberry genotypes, up to 23% of the detached fruit was immature in the southern highbush blueberry genotypes. Application of the abscission agents methyl jasmonate (MeJa; 20 mm) and ethephon (1000 mg·L−1) reduced the time required for fruit detachment on mechanical shaking by up to 5-fold. Together, these data indicate that the mechanical shaking device developed here is an effective tool for studying fruit detachment in blueberry. This instrument has potential applications in blueberry research programs evaluating fruit production. It can be used in breeding programs to aid in the selection of genotypes with fruit detachment characteristics that are potentially better suited for mechanical harvesting, and also in programs involving the screening and evaluation of abscission agents in blueberry.

scholarly journals Standardizing Postharvest Quality and Biochemical Phenotyping for Precise Population Comparison

HortScience ◽  
2010 ◽  
Vol 45 (9) ◽  
pp. 1307-1309 ◽  
Author(s):  
David Rudell
Keyword(s):  
Fruit Quality ◽  
Postharvest Quality ◽  
Dynamic Nature ◽  
Breeding Programs ◽  
Germplasm Collections ◽  
Population Comparison ◽  
Standardize Evaluation ◽  
Different Populations ◽  
Marker Discovery ◽  
Selection Of

Selection of plant material with desired traits from different populations can be difficult, if not impossible, when evaluation methods are not standardized. Discerning comparable fruit postharvest traits among populations is particularly problematic because techniques and reporting protocols are often unique or non-existent for those crucial to quality and storability. Moreover, difficulties evaluating postharvest traits may be exacerbated by the dynamic nature of fruit ripening, introducing error even into intrapopulation comparisons. With the advent of biochemical phenotyping of fruit quality, opportunities to standardize evaluation of these and other important fruit postharvest traits are materializing. Standardized trait evaluation among breeding programs and, most importantly, germplasm collections is expected to allow more precise comparison between populations, expediting integration of economically important fruit quality traits into new populations as well as facilitating marker discovery.

scholarly journals Molecular Identification of Pm4 Powdery Mildew Resistant Gene in Oat

2018 ◽  
Vol 46 (2) ◽  
pp. 350-355 ◽  
Author(s):  
Sylwia Magdalena OKOŃ ◽  
Tomasz OCIEPA ◽  
Aleksandra NUCIA
Keyword(s):  
Molecular Markers ◽  
Powdery Mildew ◽  
Correlation Coefficient ◽  
Molecular Identification ◽  
Marker Assisted Selection ◽  
Mapping Population ◽  
Breeding Programs ◽  
Resistant Gene ◽  
Modern Breeding ◽  
Selection Of

The selection of specific plants with desirable traits supported by molecular markers is one of the most important tools in modern breeding programs, which lead to reduce time and cost of selection. The aim of presented study was identification of dominant markers associated with Pm4 powdery mildew resistant gene in oat. To identify dominant silicoDArT markers for Pm4 gene, F2 mapping population ‘Av1860’ × ‘Fuchs’ were analyzed using DArTseq methodology. Among obtained 46 230 silicoDArT markers, 126 markers were high correlated with resistance to powdery mildew in oat conditioned by Pm4 gene. Among selected markers, 48 sequences have been chosen for potential conversion into specific STS markers. Finally, only 20 were suitable for primer design. As a result, 5 converted markers amplified expected products in resistant bulks, 3 of them segregated according to resistance in the whole population and shoved high correlation coefficient between marker and phenotype observation. Converted markers based on PCR could be used for identification of Pm4 gene in oat. Obtained results confirm the possibility of converting silicoDArT markers into PCR-based technique, which can be used in marker assisted selection (MAS).

ESTIMASI NILAI HERITABILITAS BOBOT IKAN MAS VARIETAS PUNTEN DALAM PROGRAM SELEKSI INDIVIDU

2016 ◽  
Vol 11 (3) ◽  
pp. 217
Author(s):  
Estu Nugroho ◽  
Budi Setyono ◽  
Mochammad Su’eb ◽  
Tri Heru Prihadi
Keyword(s):  
Body Weight ◽  
Selection Response ◽  
Base Population ◽  
Breeding Programs ◽  
Male And Female ◽  
Body Weight Trait ◽  
Population Selection ◽  
Selection For ◽  
Weight Trait ◽  
Selection Of

Program pemuliaan ikan mas varietas Punten dilakukan dengan seleksi individu terhadap karakter bobot ikan. Pembentukan populasi dasar untuk kegiatan seleksi dilakukan dengan memijahkan secara massal induk ikan mas yang terdiri atas 20 induk betina dan 21 induk jantan yang dikoleksi dari daerah Punten, Kepanjen (delapan betina dan enam jantan), Kediri (tujuh betina dan 12 jantan), Sragen (27 betina dan 10 jantan), dan Blitar (15 betina dan 11 jantan). Larva umur 10 hari dipelihara selama empat bulan. Selanjutnya dilakukan penjarangan sebesar 50% dan benih dipelihara selama 14 bulan untuk dilakukan seleksi dengan panduan hasil sampling 250 ekor individu setiap populasi. Seleksi terhadap calon induk dilakukan saat umur 18 bulan pada populasi jantan dan betina secara terpisah dengan memilih berdasarkan 10% bobot ikan yang terbaik. Calon induk yang terseleksi kemudian dipelihara hingga matang gonad, kemudian dipilih sebanyak 150 pasang dan dipijahkan secara massal. Didapatkan respons positif dari hasil seleksi berdasarkan bobot ikan, yaitu 49,89 g atau 3,66% (populasi ikan jantan) dan 168,47 g atau 11,43% (populasi ikan betina). Nilai heritabilitas untuk bobot ikan adalah 0,238 (jantan) dan 0,505 (betina).Punten carp breeding programs were carried out by individual selection for body weight trait. The base population for selection activities were conducted by mass breeding of parent consisted of 20 female and 21 male collected from area Punten, eight female and six male (Kepanjen), seven female and 12 male (Kediri), 27 female and 10 male (Sragen), 15 female and 11 male (Blitar). Larvae 10 days old reared for four moths. Then after spacing out 50% of total harvest, the offspring reared for 14 months for selection activity based on the sampling of 250 individual each population. Selection of broodstock candidates performed since 18 months age on male and female populations separately by selecting based on 10% of fish with best body weight. Candidates selected broodstocks were then maintained until mature. In oder to produce the next generation 150 pairs were sets and held for mass spawning. The results revealed that selection response were positive, 49.89 g (3.66%) for male and 168.47 (11.43%) for female. Heritability for body weight is 0.238 (male) and 0.505 (female).

scholarly journals Combining metabolomic and transcriptomic approaches to assess and improve crop quality traits

2021 ◽  
Vol 2 (1) ◽  
Author(s):  
Delphine M. Pott ◽  
Sara Durán-Soria ◽  
Sonia Osorio ◽  
José G. Vallarino
Keyword(s):  
Quality Trait ◽  
Quality Traits ◽  
Crop Species ◽  
Breeding Programs ◽  
Crop Quality ◽  
Crop Varieties ◽  
New Genes ◽  
Technical Advances ◽  
Health Promoting ◽  
Selection Of

AbstractPlant quality trait improvement has become a global necessity due to the world overpopulation. In particular, producing crop species with enhanced nutrients and health-promoting compounds is one of the main aims of current breeding programs. However, breeders traditionally focused on characteristics such as yield or pest resistance, while breeding for crop quality, which largely depends on the presence and accumulation of highly valuable metabolites in the plant edible parts, was left out due to the complexity of plant metabolome and the impossibility to properly phenotype it. Recent technical advances in high throughput metabolomic, transcriptomic and genomic platforms have provided efficient approaches to identify new genes and pathways responsible for the extremely diverse plant metabolome. In addition, they allow to establish correlation between genotype and metabolite composition, and to clarify the genetic architecture of complex biochemical pathways, such as the accumulation of secondary metabolites in plants, many of them being highly valuable for the human diet. In this review, we focus on how the combination of metabolomic, transcriptomic and genomic approaches is a useful tool for the selection of crop varieties with improved nutritional value and quality traits.

scholarly journals Mapping QTL for spike fertility and related traits in two doubled haploid wheat (Triticum aestivum L.) populations

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Nicole Pretini ◽  
Leonardo S. Vanzetti ◽  
Ignacio I. Terrile ◽  
Guillermo Donaire ◽  
Fernanda G. González
Keyword(s):  
Doubled Haploid ◽  
Marker Assisted Selection ◽  
Triticum Aestivum L ◽  
Physiological Model ◽  
Yield Potential ◽  
Breeding Programs ◽  
Per Se ◽  
Genomic Regions ◽  
Fertility Traits ◽  
Selection Of

Abstract Background In breeding programs, the selection of cultivars with the highest yield potential consisted in the selection of the yield per se, which resulted in cultivars with higher grains per spike (GN) and occasionally increased grain weight (GW) (main numerical components of the yield). In this study, quantitative trait loci (QTL) for GW, GN and spike fertility traits related to GN determination were mapped using two doubled haploid (DH) populations (Baguette Premium 11 × BioINTA 2002 and Baguette 19 × BioINTA 2002). Results In total 305 QTL were identified for 14 traits, out of which 12 QTL were identified in more than three environments and explained more than 10% of the phenotypic variation in at least one environment. Eight hotspot regions were detected on chromosomes 1A, 2B, 3A, 5A, 5B, 7A and 7B in which at least two major and stable QTL sheared confidence intervals. QTL on two of these regions (R5A.1 and R5A.2) have previously been described, but the other six regions are novel. Conclusions Based on the pleiotropic analysis within a robust physiological model we conclude that two hotspot genomic regions (R5A.1 and R5A.2) together with the QGW.perg-6B are of high relevance to be used in marker assisted selection in order to improve the spike yield potential. All the QTL identified for the spike related traits are the first step to search for their candidate genes, which will allow their better manipulation in the future.

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