scholarly journals Identification of the Group III WRKY Subfamily and the Functional Analysis of GhWRKY53 in Gossypium hirsutum L.

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1235
Author(s):  
Dongjie Yang ◽  
Yuanyuan Liu ◽  
Hailiang Cheng ◽  
Qiaolian Wang ◽  
Limin Lv ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Cotton Fiber ◽  
Fiber Quality ◽  
Fiber Development ◽  
Amino Acid Sequences ◽  
Fiber Formation ◽  
Biotic And Abiotic Stress ◽  
Motif Analysis ◽  
Gossypium Hirsutum L ◽  
Group Iii

WRKY transcription factors had multiple functions in plant secondary metabolism, leaf senescence, fruit ripening, adaptation to biotic and abiotic stress, and plant growth and development. However, knowledge of the group III WRKY subfamily in fiber development in upland cotton (Gossypium hirsutum L.) is largely absent. Previous studies have shown that there were 21 putative group III WRKY members in G. hirsutum L. These putative amino acid sequences from the III WRKY group were phylogenetically clustered into three clades. Multiple alignment, conservative motif analysis, and gene structure analysis showed that the members clustered together in the phylogenetic tree had similar motifs and gene structures. Expression pattern analysis revealed that variation in the expression levels of these genes in different tissues and fiber development stages. To better understand the functions of putative group III WRKY genes in G. hirsutum L., we selected the cotton fiber initiation-related gene GhWRKY53 for cloning and functional identification. The subcellular localization experiment of GhWRKY53 in Nicotiana tabacum leaves showed that it was located in the nucleus. The heterologous expression of GhWRKY53 in Arabidopsis thaliana could significantly increase the density of trichomes. Twelve proteins that interacted with GhWRKY53 were screened from the cotton fiber cDNA library by yeast two-hybrid experiment. This study findings lay a foundation for further research on the role of the GhWRKY53 during cotton fiber development and provide a new insight for further studying putative group III WRKY genes in G. hirsutum L. Our research results also provide vital information for the genetic mechanism of high-quality cotton fiber formation and essential genetic resources for cotton fiber quality improvement.

scholarly journals Functional analysis of GhCHS, GhANR and GhLAR in colored fiber formation of Gossypium hirsutum L.

2019 ◽  
Author(s):  
Gao Jianfang ◽  
Shen Li ◽  
Yuan Jingli ◽  
Zheng Hongli ◽  
Su Quansheng ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Cotton Fiber ◽  
Fiber Formation ◽  
Gossypium Hirsutum L ◽  
Expression Levels ◽  
Color Formation ◽  
Colored Cotton ◽  
Natural Colored Cotton ◽  
Key Genes ◽  
Color Depth

Abstract Background The formation of natural colored fibers mainly results from the accumulation of different anthocyanidins and their derivatives in the fibers of Gossypium hirsutum L. Chalcone synthase (CHS) is the first committed enzyme of flavonoid biosynthesis, and anthocyanidins are transported into fiber cell after biosynthesis mainly by Anthocyanidin reductase (ANR) and Leucoanthocyanidin reductase (LAR) to present diverse colors with distinct stability. The biochemical and molecular mechanism of pigment formation in natural colored cotton fiber is not clear. Results The three key genes of GhCHS , GhANR and GhLAR were predominantly expressed in the developing fibers of colored cotton. In the GhCHSi , GhANRi and GhLARi transgenic cottons, the expression levels of GhCHS , GhANR and GhLAR significantly decreased in the developing cotton fiber, negatively correlated with the content of anthocyanidins and the color depth of cotton fiber. In colored cotton Zongxu1 (ZX1) and the GhCHSi , GhANRi and GhLARi transgenic lines of ZX1, HZ and ZH, the anthocyanidin contents of the leaves, cotton kernels, the mixture of fiber and seedcoat were all changed and positively correlated with the fiber color. Conclusion The three genes of GhCHS , GhANR and GhLAR were predominantly expressed early in developing colored cotton fibers and identified to be a key genes of cotton fiber color formation. The expression levels of the three genes affected the anthocyanidin contents and fiber color depth. So the three genes played a crucial part in cotton fiber color formation and has important significant to improve natural colored cotton quality and create new colored cotton germplasm resources by genetic engineering.

scholarly journals Functional analysis of GhCHS, GhANR and GhLAR in colored fiber formation of Gossypium hirsutum L.

2019 ◽  
Author(s):  
Gao Jianfang ◽  
Shen Li ◽  
Yuan Jingli ◽  
Zheng Hongli ◽  
Su Quansheng ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Cotton Fiber ◽  
Fiber Formation ◽  
Gossypium Hirsutum L ◽  
Expression Levels ◽  
Color Formation ◽  
Colored Cotton ◽  
Natural Colored Cotton ◽  
Key Genes ◽  
Color Depth

Abstract Background The formation of natural colored fibers mainly results from the accumulation of different anthocyanidins and their derivatives in the fibers of Gossypium hirsutum L. Chalcone synthase (CHS) is the first committed enzyme of flavonoid biosynthesis, and anthocyanidins are transported into fiber cell after biosynthesis mainly by Anthocyanidin reductase (ANR) and Leucoanthocyanidin reductase (LAR) to present diverse colors with distinct stability. The biochemical and molecular mechanism of pigment formation in natural colored cotton fiber is not clear. Results The three key genes of GhCHS , GhANR and GhLAR were predominantly expressed in the developing fibers of colored cotton. In the GhCHSi , GhANRi and GhLARi transgenic cottons, the expression levels of GhCHS , GhANR and GhLAR significantly decreased in the developing cotton fiber, negatively correlated with the content of anthocyanidins and the color depth of cotton fiber. In colored cotton Zongxu1 (ZX1) and the GhCHSi , GhANRi and GhLARi transgenic lines of ZX1, HZ and ZH, the anthocyanidin contents of the leaves, cotton kernels, the mixture of fiber and seedcoat were all changed and positively correlated with the fiber color. Conclusion The three genes of GhCHS , GhANR and GhLAR were predominantly expressed early in developing colored cotton fibers and identified to be a key genes of cotton fiber color formation. The expression levels of the three genes affected the anthocyanidin contents and fiber color depth. So the three genes played a crucial part in cotton fiber color formation and has important significant to improve natural colored cotton quality and create new colored cotton germplasm resources by genetic engineering.

scholarly journals Functional analysis of GhCHS, GhANR and GhLAR in colored fiber formation of Gossypium hirsutum L.

2019 ◽  
Author(s):  
Gao Jianfang ◽  
Shen Li ◽  
Yuan Jingli ◽  
Zheng Hongli ◽  
Su Quansheng ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Cotton Fiber ◽  
Fiber Formation ◽  
Gossypium Hirsutum L ◽  
Expression Levels ◽  
Color Formation ◽  
Colored Cotton ◽  
Natural Colored Cotton ◽  
Key Genes ◽  
Color Depth

Abstract Background The formation of natural colored fibers mainly results from the accumulation of different anthocyanidins and their derivatives in the fibers of Gossypium hirsutum L. Chalcone synthase (CHS) is the first committed enzyme of flavonoid biosynthesis, and anthocyanidins are transported into fiber cell after biosynthesis mainly by Anthocyanidin reductase (ANR) and Leucoanthocyanidin reductase (LAR) to present diverse colors with distinct stability. The biochemical and molecular mechanism of pigment formation in natural colored cotton fiber is not clear. Results The three key genes of GhCHS , GhANR and GhLAR were predominantly expressed in the developing fibers of colored cotton. In the GhCHSi , GhANRi and GhLARi transgenic cottons, the expression levels of GhCHS , GhANR and GhLAR significantly decreased in the developing cotton fiber, negatively correlated with the content of anthocyanidins and the color depth of cotton fiber. In colored cotton Zongxu1 (ZX1) and the GhCHSi , GhANRi and GhLARi transgenic lines of ZX1, HZ and ZH, the anthocyanidin contents of the leaves, cotton kernels, the mixture of fiber and seedcoat were all changed and positively correlated with the fiber color. Conclusion The three genes of GhCHS , GhANR and GhLAR were predominantly expressed early in developing colored cotton fibers and identified to be a key genes of cotton fiber color formation. The expression levels of the three genes affected the anthocyanidin contents and fiber color depth. So the three genes played a crucial part in cotton fiber color formation and has important significant to improve natural colored cotton quality and create new colored cotton germplasm resources by genetic engineering.

scholarly journals QUALIDADE DA FIBRA DO ALGODOEIRO BRS VERDE IRRIGADO COM ÁGUAS DE DIFERENTES NÍVEIS SALINOS

Irriga ◽  
2018 ◽  
Vol 1 (1) ◽  
pp. 209 ◽  
Author(s):  
Francisco Italo Gomes Paiva ◽  
Marcelo Tavares Gurgel ◽  
Francisco De Assis de Oliveira ◽  
Lucas Ramos Da Costa ◽  
Andygley Fernandes Mota ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Gossypium Hirsutum ◽  
Irrigation Water ◽  
Cotton Fiber ◽  
Fiber Quality ◽  
Short Fiber ◽  
Gossypium Hirsutum L ◽  
Third Phase ◽  
Salinity Levels

QUALIDADE DA FIBRA DO ALGODOEIRO BRS VERDE IRRIGADO COM ÁGUAS DE DIFERENTES NÍVEIS SALINOS FRANCISCO ITALO GOME PAIVA1; MARCELO TAVARES GURGEL2; FRANCISCO DE ASSIS DE OLIVEIRA3 ANDYGLEY FERNANDES MOTA4;LUCAS RAMOS DA COSTA 5; HERMINIO SABINO DE OLIVEIRA JUNIOR6 1Engenheiro Agrônomo, Mestrando em Manejo de solo e agua – UFERSA/RN, [email protected];2 Engenheiro Agrônomo, Prof. Doutor, Manejo solo e agua – UFERSA/RN, [email protected];3Engenheiro Agrônomo, Prof. Doutor, Manejo solo e agua – UFERSA/RN, [email protected];4Engenheiro Agrônomo, Mestre, Doutorando, UFCG – Campina Grande/PB, [email protected];5Engenheiro Agrônomo, Mestre, Doutorando – UFERSA/RN, [email protected];6Gestor Ambiental, Mestre – UFERSA/RN, [email protected].  1 RESUMO As fibras de algodão representam em torno de 80% das fibras utilizadas nas fiações brasileiras. Nesse contexto, o objetivo do presente estudo foi avaliar a qualidade da fibra do algodoeiro BRS verde, usando níveis de salinidade da água de irrigação. O experimento foi realizado em condições de campo na Fazenda Experimental Rafael Fernandes, de propriedade da Universidade Federal Rural do Semiárido­–UFERSA no período 21/10/11 até 06/02/12. O delineamento experimental adotado foi em blocos casualizados com seis tratamentos e cinco repetições, totalizando 30 parcelas experimentais. Os tratamentos resultaram das combinações de três tipos de condutividade elétrica da água de irrigação (S1- 0,55; S2-2,16 e S3-3,53 dS m-1) de acordo com as fases de desenvolvimento da cultura (T1- S1S1S1; T2- S2S2S2; T3- S3S3S3; T4- S1S2S2; T5- S1S2S3 e T6- S1S3S3), sendo a primeira fase do semeio até 30 dias; a segunda fase inicia-se dos 31 aos 90 dias; e a terceira fase dos 91 a colheita. As variáveis analisadas foram: porcentagem de fibra, comprimento da fibra, uniformidade do comprimento, maturidade, resistência, índice micronaire, índice de fibras curtas, elongação de ruptura e índice de fiabilidade. No geral, não houve efeito da salinidade da água de irrigação sobre a qualidade de fibra do algodão BRS verde, exceto para a característica elongamento da ruptura (ELG). Deste modo, torna-se viável o uso de água com salinidade de (3,5 dS m-1) em todo o ciclo da cultura considerando as condições de realização do estudo. Palavras-chave: Gossypium hirsutum L.. Condutividade elétrica. Características tecnológicas da fibra.  PAIVA,F.I.G.;GURGEL,M.T.;OLIVEIRA,F.A.;MOTA,A.F.;COSTA,L.R.;JUNIOR,H.S.EQUALITY OF COTTON FIBER BRS GREEN IRRIGATED WITH DIFFERENT SALT LEVELS    2 ABSTRACT Cotton fibers represent around 80% of the fibers used in spinning mills in Brazil. In this context, the aim of this study was to evaluate the quality of the cotton fiber BRS green, using salinity levels of irrigation water. The experiment was conducted under field condition sat the Experimental Farm Rafael Fernandes, owned by Federal Rural University of the semiarid – UFERSA the period 21/10/11 to 06/02/12. The treatments consisted of combinations of three types of electrical conductivity of irrigation water (S1- 0,55; S2-2,16 and S3-3,53 dS m-1) according to the development stages of culture T1- S1S1S1; T2- S2S2S2; T3- S3S3S3; T4- S1S2S2; T5- S1S2S3; T6- S1S3S3, the first phase being 30 days from the sowing; the second stage starts from 31 to 90 days; and the third phase of the 91 harvest. The variables analyzed were: fiber percentage, fiber length, length uniformity, maturity, strength, micronaire, short fiber index, elongation at break and reliability index. Overall, there was no effect of irrigation water salinity on the fiber quality of cotton BRS green, except for the elongation characteristic of rupture (ELG). Thus, it becomes feasible to use water with salinity (3,5 dS m-1) throughout the crop cycle considering the conditions of the study. Keywords: Gossypium hirsutum L.. Electrical conductivity. technological characteristics of the fiber.

scholarly journals Comparative transcriptome analysis of fiber and nonfiber tissues to identify the genes preferentially expressed in fiber development in Gossypium hirsutum

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jiangtao Yang ◽  
Lihua Gao ◽  
Xiaojing Liu ◽  
Xiaochun Zhang ◽  
Xujing Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gossypium Hirsutum ◽  
Rna Sequencing ◽  
Transcriptome Analysis ◽  
Cotton Fiber ◽  
Upland Cotton ◽  
Fiber Quality ◽  
Fiber Development ◽  
Comparative Transcriptome

AbstractCotton is an important natural fiber crop and economic crop worldwide. The quality of cotton fiber directly determines the quality of cotton textiles. Identifying cotton fiber development-related genes and exploring their biological functions will not only help to better understand the elongation and development mechanisms of cotton fibers but also provide a theoretical basis for the cultivation of new cotton varieties with excellent fiber quality. In this study, RNA sequencing technology was used to construct transcriptome databases for different nonfiber tissues (root, leaf, anther and stigma) and fiber developmental stages (7 days post-anthesis (DPA), 14 DPA, and 26 DPA) of upland cotton Coker 312. The sizes of the seven transcriptome databases constructed ranged from 4.43 to 5.20 Gb, corresponding to approximately twice the genome size of Gossypium hirsutum (2.5 Gb). Among the obtained clean reads, 83.32% to 88.22% could be compared to the upland cotton TM-1 reference genome. By analyzing the differential gene expression profiles of the transcriptome libraries of fiber and nonfiber tissues, we obtained 1205, 1135 and 937 genes with significantly upregulated expression at 7 DPA, 14 DPA and 26 DPA, respectively, and 124, 179 and 213 genes with significantly downregulated expression. Subsequently, Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathway analyses were performed, which revealed that these genes were mainly involved in catalytic activity, carbohydrate metabolism, the cell membrane and organelles, signal transduction and other functions and metabolic pathways. Through gene annotation analysis, many transcription factors and genes related to fiber development were screened. Thirty-six genes were randomly selected from the significantly upregulated genes in fiber, and expression profile analysis was performed using qRT-PCR. The results were highly consistent with the gene expression profile analyzed by RNA-seq, and all of the genes were specifically or predominantly expressed in fiber. Therefore, our RNA sequencing-based comparative transcriptome analysis will lay a foundation for future research to provide new genetic resources for the genetic engineering of improved cotton fiber quality and for cultivating new transgenic cotton germplasms for fiber quality improvement.

Construction of genetic map and QTL analysis of fiber quality traits for Upland cotton (Gossypium hirsutum L.)

Euphytica ◽  
2014 ◽  
Vol 201 (2) ◽  
pp. 195-213 ◽  
Author(s):  
Shiyi Tang ◽  
Zhonghua Teng ◽  
Tengfei Zhai ◽  
Xiaomei Fang ◽  
Fang Liu ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Genetic Map ◽  
Qtl Analysis ◽  
Upland Cotton ◽  
Fiber Quality ◽  
Quality Traits ◽  
Gossypium Hirsutum L ◽  
Fiber Quality Traits

scholarly journals Ameliorative effects of potassium nutrition on yield and fiber quality characteristics of cotton (Gossypium hirsutum L.) under NaCl stress

2017 ◽  
Vol 36 (01) ◽  
pp. 51-58 ◽  
Author(s):  
Muhammad Ashraf ◽  
Sher Muhammad Shahzad ◽  
Muhammad Imtiaz ◽  
Muhammad Shahid Rizwan ◽  
Muhammad Mahzar Iqbal
Keyword(s):  
Gossypium Hirsutum ◽  
Fiber Quality ◽  
Nacl Stress ◽  
Quality Characteristics ◽  
Gossypium Hirsutum L ◽  
Potassium Nutrition
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