scholarly journals A Novel Isolate of Bacillus cereus Promotes Growth in Tomato and Inhibits Clavibacter michiganensis Infection under Greenhouse Conditions

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 506
Author(s):  
Nallely Solano-Alvarez ◽  
Juan Antonio Valencia-Hernández ◽  
Enrique Rico-García ◽  
Irineo Torres-Pacheco ◽  
Rosalía Virginia Ocampo-Velázquez ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Fossil Fuels ◽  
New Technologies ◽  
Ricinus Communis ◽  
Dry Weight ◽  
Plant Growth Promoting Bacteria ◽  
Clavibacter Michiganensis ◽  
Tomato Plants ◽  
Canker Disease ◽  
Plant Growth Promoting

The need to produce food in a sustainable way to counteract the effects of excessive use of agrochemicals opens the door to the generation of new technologies that are not based on fossil fuels and are less toxic to ecosystems. Plant growth-promoting bacteria (PGPB) could represent an alternative to chemical biofertilizers and pesticides offering protection for biotic and abiotic stresses. In this work, a bacterial isolate from roots of castor bean (Ricinus communis) was identified and named as Bacillus cereus strain “Amazcala” (B.c-A). This isolate displayed the ability to solubilize inorganic phosphate and produce gibberellic acid (GA3). Moreover, this bacterium provided significant increases in height, stem width, dry weight, and total chlorophyll content in tomato plants. Interestingly, B.c-A also significantly decreased the severity of bacterial canker disease on tomato caused by Clavibacter michiganensis (Cmm) in preventive disease assays under greenhouse conditions. Based on our results, B.c-A can be considered as PGPB and a useful tool in Cmm disease control on tomato plant under greenhouse conditions.

scholarly journals Efficacy of Plant Growth-Promoting Bacteria Bacillus cereus YN917 for Biocontrol of Rice Blast

2021 ◽  
Vol 12 ◽  
Author(s):  
Hu Zhou ◽  
Zuo-hua Ren ◽  
Xue Zu ◽  
Xi-yue Yu ◽  
Hua-jun Zhu ◽  
...  
Keyword(s):  
Disease Prevention ◽  
Plant Growth ◽  
Bacillus Cereus ◽  
Rice Blast ◽  
Growth Promotion ◽  
Gene Clusters ◽  
Plant Growth Promoting Bacteria ◽  
Detached Leaf ◽  
Plant Growth Promoting ◽  
Growth Promoting

Bacillus cereus YN917, obtained from a rice leaf with remarkable antifungal activity against Magnaporthe oryzae, was reported in our previous study. The present study deciphered the possible biocontrol properties. YN917 strain exhibits multiple plant growth-promoting and disease prevention traits, including production of indole-3-acetic acid (IAA), ACC deaminase, siderophores, protease, amylase, cellulase, and β-1,3-glucanase, and harboring mineral phosphate decomposition activity. The effects of the strain YN917 on growth promotion and disease prevention were further evaluated under detached leaf and greenhouse conditions. The results revealed that B. cereus YN917 can promote seed germination and seedling plant growth. The growth status of rice plants was measured from the aspects of rice plumule, radicle lengths, plant height, stem width, root lengths, fresh weights, dry weights, and root activity when YN917 was used as inoculants. YN917 significantly reduced rice blast severity under detached leaf and greenhouse conditions. Genome analysis revealed the presence of gene clusters for biosynthesis of plant promotion and antifungal compounds, such as IAA, tryptophan, siderophores, and phenazine. In summary, YN917 can not only be used as biocontrol agents to minimize the use of chemical substances in rice blast control, but also can be developed as bio-fertilizers to promote the rice plant growth.

scholarly journals Protection of Tomato Seedlings against Infection by Pseudomonas syringae pv. Tomato by Using the Plant Growth-Promoting Bacterium Azospirillum brasilense

2002 ◽  
Vol 68 (6) ◽  
pp. 2637-2643 ◽  
Author(s):  
Yoav Bashan ◽  
Luz E. de-Bashan
Keyword(s):  
Plant Growth ◽  
Pseudomonas Syringae ◽  
Azospirillum Brasilense ◽  
Dry Weight ◽  
Tomato Plants ◽  
Bacterial Speck ◽  
Plant Growth Promoting ◽  
Bacterial Speck Disease ◽  
Growth Promoting ◽  
Mist Chamber

ABSTRACT Pseudomonas syringae pv. tomato, the causal agent of bacterial speck of tomato, and the plant growth-promoting bacterium Azospirillum brasilense were inoculated onto tomato plants, either alone, as a mixed culture, or consecutively. The population dynamics in the rhizosphere and foliage, the development of bacterial speck disease, and their effects on plant growth were monitored. When inoculated onto separate plants, the A. brasilense population in the rhizosphere of tomato plants was 2 orders of magnitude greater than the population of P. syringae pv. tomato (107 versus 105 CFU/g [dry weight] of root). Under mist chamber conditions, the leaf population of P. syringae pv. tomato was 1 order of magnitude greater than that of A. brasilense (107 versus 106 CFU/g [dry weight] of leaf). Inoculation of seeds with a mixed culture of the two bacterial strains resulted in a reduction of the pathogen population in the rhizosphere, an increase in the A. brasilense population, the prevention of bacterial speck disease development, and improved plant growth. Inoculation of leaves with the mixed bacterial culture under mist conditions significantly reduced the P. syringae pv. tomato population and significantly decreased disease severity. Challenge with P. syringae pv. tomato after A. brasilense was established in the leaves further reduced both the population of P. syringae pv. tomato and disease severity and significantly enhanced plant development. Both bacteria maintained a large population in the rhizosphere for 45 days when each was inoculated separately onto tomato seeds (105 to 106 CFU/g [dry weight] of root). However, P. syringae pv. tomato did not survive in the rhizosphere in the presence of A. brasilense. Foliar inoculation of A. brasilense after P. syringae pv. tomato was established on the leaves did not alleviate bacterial speck disease, and A. brasilense did not survive well in the phyllosphere under these conditions, even in a mist chamber. Several applications of a low concentration of buffered malic acid significantly enhanced the leaf population of A. brasilense (>108 CFU/g [dry weight] of leaf), decreased the population of P. syringae pv. tomato to almost undetectable levels, almost eliminated disease development, and improved plant growth to the level of uninoculated healthy control plants. Based on our results, we propose that A. brasilense be used in prevention programs to combat the foliar bacterial speck disease caused by P. syringae pv. tomato.

scholarly journals Growth response of wheat cultivars to bacterial inoculation in calcareous soil

2010 ◽  
Vol 56 (No. 12) ◽  
pp. 570-573 ◽  
Author(s):  
D. Egamberdieva
Keyword(s):  
Plant Growth ◽  
Calcareous Soil ◽  
Dry Weight ◽  
Plant Growth Promoting Bacteria ◽  
Wheat Cultivars ◽  
Bacterial Strains ◽  
Bacterial Inoculation ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Selection Of

In this study the plant growth-promoting bacteria were analysed for their growth-stimulating effects on two wheat cultivars. The investigations were carried out in pot experiments using calcareous soil. The results showed that bacterial strains Pseudomonas spp. NUU1 and P. fluorescens NUU2 were able to colonize the rhizosphere of both wheat cultivars. Their plant growth-stimulating abilities were affected by wheat cultivars. The bacterial strains Pseudomonas sp. NUU1 and P. fluorescens NUU2 significantly stimulated the shoot and root length and dry weight of wheat cv. Turon, whereas cv. Residence was less affected by bacterial inoculation. The results of our study suggest that inoculation of wheat with Pseudomonas strains can improve plant growth in calcareous soil and it depends upon wheat cultivars. Prior to a selection of good bacterial inoculants, it is recommended to select cultivars that benefit from association with these bacteria.

scholarly journals Synergistic and offset effects of fungal species combinations on plant performance

2021 ◽  
Author(s):  
Yoshie Hori ◽  
Hiroaki Fujita ◽  
Kei Hiruma ◽  
Kazuhiko Narisawa ◽  
Hirokazu Toju
Keyword(s):  
Plant Growth ◽  
Synergistic Effects ◽  
Dry Weight ◽  
Fungal Species ◽  
Plant Performance ◽  
Plant Growth Promoting Bacteria ◽  
Microbial Species ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Dual Inoculation

In natural and agricultural ecosystems, survival and growth of plants depend substantially on microbes in the endosphere and rhizosphere. Although numerous studies have reported the presence of plant-growth promoting bacteria and fungi in below-ground biomes, it remains a major challenge to understand how sets of microbial species positively or negatively affect plants' performance. By conducting a series of single- and dual-inoculation experiments of 13 endophytic and soil fungi targeting a Brassicaceae plant species, we here evaluated how microbial effects on plants depend on presence/absence of co-occurring microbes. The comparison of single- and dual-inoculation experiments showed that combinations of the fungal isolates with the highest plant-growth promoting effects in single inoculations did not yield highly positive impacts on plant performance traits (e.g., shoot dry weight). In contrast, pairs of fungi including small/moderate contributions to plants in single-inoculation contexts showed the greatest effects on plants among the 78 fungal pairs examined. These results on the offset and synergistic effects of pairs of microbes suggest that inoculation experiments of single microbial species/isolates can result in the overestimation or underestimation of microbial functions in multi-species contexts. Because keeping single-microbe systems in outdoor conditions is impractical, designing sets of microbes that can maximize performance of crop plants is an important step for the use of microbial functions in sustainable agriculture.

Azospirillum plant growth-promoting strains are nonpathogenic on tomato, pepper, cotton, and wheat

1998 ◽  
Vol 44 (2) ◽  
pp. 168-174 ◽  
Author(s):  
Yoav Bashan
Keyword(s):  
Plant Growth ◽  
Plant Species ◽  
Pseudomonas Syringae ◽  
Xanthomonas Campestris ◽  
Plant Pathogens ◽  
Dry Weight ◽  
Plant Growth Promoting Bacteria ◽  
Disease Symptoms ◽  
Plant Growth Promoting ◽  
Growth Promoting

Six strains of Azospirillum belonging to five species of plant growth-promoting bacteria (A. brasilense, A. lipoferum, A. amazonense, A. irakense, and A. halopraeference) did not cause visible disease symptoms on the roots or leaves of tomato, pepper, cotton, and wheat, failed to inhibit seed germination, and did not reduce plant dry weight when seven standard techniques for the inoculation of plant pathogens were used. Similar inoculation conditions with plant pathogens (Pseudomonas syringae pv. tomato, Xanthomonas campestris pv. vesicatoria, Xanthomonas campestris pv. translucens, and Xanthomonas campestris pv. malvacearum) induced typical disease symptoms. None of Azospirillum strains caused the hypersensitive reaction on eggplant, whereas all pathogens did. All Azospirillum strains increased phytoalexin production in all disease-resistant plant species to moderate levels, but the levels were significantly lower than those induced by the compatible pathogens. The various phytoalexins produced in plants had the capacity to inhibit growth of all Azospirillum strains. Azospirillum amazonense, A. irakense, and A. halopraeference had no apparent effect on plant growth, while A. brasilense and A. lipoferum increased the dry weight of all plant species. Under partial mist conditions, all Azospirillum strains were capable of colonizing leaf surfaces (103-107 cfu/g dry weight) regardless of the plant species. These results provide experimental evidence that Azospirillum sp. might be considered safe for the inoculation of several plant species.Key words: Azospirillum, beneficial bacteria, environmental protection, plant inoculation, plant growth-promoting bacteria.

Plant growth-promoting bacteria that decrease heavy metal toxicity in plants

2000 ◽  
Vol 46 (3) ◽  
pp. 237-245 ◽  
Author(s):  
Genrich I Burd ◽  
D George Dixon ◽  
Bernard R Glick
Keyword(s):  
Heavy Metal ◽  
Plant Growth ◽  
Indian Mustard ◽  
Dry Weight ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Plant Growth Promoting ◽  
High Concentrations ◽  
Kluyvera Ascorbata ◽  
Nickel Lead

Kluyvera ascorbata SUD165 and a siderophore-overproducing mutant of this bacterium, K. ascorbata SUD165/26, were used to inoculate tomato, canola, and Indian mustard seeds which were then grown in soil for 25-42 days in the presence of either nickel, lead, or zinc. The parameters that were monitored included plant wet and dry weight, protein and chlorophyll content in the plant leaves, and concentration of heavy metal in the plant roots and shoots. As indicated by a decrease in the measured values of these parameters, in all instances, plant growth was inhibited by the presence of the added metal. Both bacterial strains were effective, although not always to a statistically significant extent, at relieving a portion of the growth inhibition caused by the metals. In most cases, the siderophore overproducing mutant K. ascorbata 165/26 exerted a more pronounced effect on plant growth than did the wild-type bacterium K. ascorbata SUD165. The data suggest that the ability of these bacteria to protect plants against the inhibitory effects of high concentrations of nickel, lead, and zinc is related to the bacteria providing the plants with sufficient iron.Key words: phytoremediation, heavy metals, siderophores, Kluyvera ascorbata.

scholarly journals Mineral Phosphate Solubilizing Bacteria Isolated from Various Plant Rhizosphere under Different Aluminum Content

2015 ◽  
Vol 10 (2) ◽  
Author(s):  
Dolly Iriani Damarjaya ◽  
Jaka Widada ◽  
Keishi Senoo ◽  
Masaya Nishiyama ◽  
Shigeto Otsuka
Keyword(s):  
Dry Weight ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Phosphate Solubilizing Bacteria ◽  
Gene Sequence Analysis ◽  
Mineral Phosphate ◽  
Plant Growth Promoting ◽  
The Media ◽  
Formation Method ◽  
Phosphate Solubilizing

The objectives of this study was to isolate and characterize the mineral phosphate solubilizing bacteriafrom rhizosphere and evaluate their potential as plant growth promoting bacteria in Al-toxic soils. The halozone formation method was used to isolate PSB using the media containing insoluble phosphates (Ca-P or Al-P)as a source of phosphate. Eight of acid and Al-tolerant PSB isolates that were able to solubilize Ca-P wereobtained from rhizosphere of clover, wheat, corn, and sunflower grown in Al-toxic soil. Identification of theisolates based on the 16S rRNA gene sequence analysis demonstrated that the isolates were strains of Burkholderia(5 strains), Pseudomonas (1 strain), Ralstonia (1 strain), and unidentified bacterium (1 strains). All PSB isolatesshowed the capability to dissolve Ca-P, and only 1 strain (Ralstonia strain) was able to dissolve Al-P in agar platemedium. The P-solubilization by these isolates was correlated with pH of medium. Inoculation of the bacterialstrains on clover on Al-toxic medium showed that all isolates increased the plant dry weight compared withuninoculated treatment. Our results showed that those PSB isolates have potential to be developed as a biofertilizerto increase the efficiency of P-inorganic fertilizer used in Al-toxic soils.

scholarly journals Production of biopolyester poly(3-hydroxybutyrate) by Bacillus cereus RCL 02, a leaf endophyte of Ricinus communis L.

2017 ◽  
Vol 7 (4) ◽  
pp. 32 ◽  
Author(s):  
Rituparna Das ◽  
Arundhati Pal ◽  
Amal K. Paul
Keyword(s):  
Bacillus Cereus ◽  
Endophytic Bacteria ◽  
Ricinus Communis ◽  
Carbon Sources ◽  
Dry Weight ◽  
Mineral Salts ◽  
Scientific Communities ◽  
Biodegradable Polyesters ◽  
Ricinus Communis L ◽  
Bacterium Bacillus

Endophytic bacteria colonizing the internal tissues of plants have attracted the attention of scientific communities in recent years for production of biodegradable polyesters like polyhydroxyalkanotaes (PHAs). A newly characterized bacterium, Bacillus cereus RCL 02 (GenBank accession no. KX458035), isolated from surface sterilized leaves of Ricinus communis L. has been explored for the production of poly(3-hydroxybutyrate) [P(3HB)], the most common PHA. As revealed by scanning electron microscopy, P(3HB) accumulating cells developed swellings or blebs and released the native granules as a function of autolysis. During growth in glucose containing mineral salts medium under batch fermentation, the isolate produced P(3HB) accounting 68% of its cell dry weight (CDW). Glucose and yeast extract when used in the ratio of 5:1, significantly influenced intracellular biopolyester accumulation (72.2%, CDW and 2.54 g/L). A further increase of polymer production (81%, CDW and 3.17 g/L) was accomplished in presence of 1.5 mM manganese as exogenous metal stress. Moreover, supplementation of the growth medium with non-conventional carbon sources especially refined sugarcane molasses further enhanced the production of both biomass (9.44 g/L) as well as polyester (83.6%, CDW and 7.89 g/L). These finding emphasises exploration of endophytic bacteria of oleaginous plants in general and R. communis L. in particular as potential but hitherto an under exploited bioresource for commercial production of biodegradable polyesters.

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