scholarly journals The Foliar Application of Rice Phyllosphere Bacteria induces Drought-Stress Tolerance in Oryza sativa (L.)

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 387
Author(s):  
Arun Kumar Devarajan ◽  
Gomathy Muthukrishanan ◽  
Jaak Truu ◽  
Marika Truu ◽  
Ivika Ostonen ◽  
...  
Keyword(s):  
Drought Stress ◽  
Stress Tolerance ◽  
Foliar Application ◽  
Oryza Sativa L ◽  
Plant Stress ◽  
Rice Cultivar ◽  
Single Strain ◽  
Rice Leaves ◽  
Stress Related Genes ◽  
Induced Tolerance

This study assessed the potential of Bacillus endophyticus PB3, Bacillus altitudinis PB46, and Bacillus megaterium PB50 to induce drought tolerance in a susceptible rice cultivar. The leaves of the potted rice plants subjected to physical drought stress for 10 days during the flowering stage were inoculated with single-strain suspensions. Control pots of irrigated and drought-stressed plants were included in the experiment for comparison. In all treatments, the plant stress-related physiochemical and biochemical changes were examined and the expression of six stress-responsive genes in rice leaves was evaluated. The colonization potential on the surface of the rice leaves and stomata of the most successful strain in terms of induced tolerance was confirmed in the gnotobiotic experiment. The plants sprayed with B. megaterium PB50 showed an elevated stress tolerance based on their higher relative water content and increased contents of total sugars, proteins, proline, phenolics, potassium, calcium, abscisic acid, and indole acetic acid, as well as a high expression of stress-related genes (LEA, RAB16B, HSP70, SNAC1, and bZIP23). Moreover, this strain improved yield parameters compared to other treatments and also confirmed its leaf surface colonization. Overall, this study indicates that the foliar application of B. megaterium PB50 can induce tolerance to drought stress in rice.

Assessment of rice (Oryza sativa L.) genotypes for drought stress tolerance using morpho-physiological indices as a screening technique

2020 ◽  
Vol 53 (1) ◽  
Author(s):  
Asma Asma ◽  
Iqbal Hussain ◽  
Muhammad Yasin Ashraf ◽  
Muhammad Arslan Ashraf ◽  
Rizwan Rasheed ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Drought Stress ◽  
Stress Tolerance ◽  
Oryza Sativa L ◽  
Screening Technique ◽  
Drought Stress Tolerance ◽  
Physiological Indices

scholarly journals In vitro Selection of Drought Stress Tolerance in Rice (Oryza sativa L.) var. Jarava

2019 ◽  
Vol 29 (1) ◽  
pp. 127-131 ◽  
Author(s):  
Omar H Obaid ◽  
SK Reddy
Keyword(s):  
Oryza Sativa ◽  
Drought Stress ◽  
Stress Tolerance ◽  
Plant Tissue ◽  
In Vitro Selection ◽  
Oryza Sativa L ◽  
Drought Stress Tolerance ◽  
Selection Of

Abstract not available Plant Tissue Cult. & Biotech. 29(1): 127-131, 2019 (June)

scholarly journals Calcium Application Enhances Drought Stress Tolerance in Sugar Beet and Promotes Plant Biomass and Beetroot Sucrose Concentration

2019 ◽  
Vol 20 (15) ◽  
pp. 3777 ◽  
Author(s):  
Seyed Abdollah Hosseini ◽  
Elise Réthoré ◽  
Sylvain Pluchon ◽  
Nusrat Ali ◽  
Bastien Billiot ◽  
...  
Keyword(s):  
Drought Stress ◽  
Plant Growth ◽  
Sugar Beet ◽  
Stress Tolerance ◽  
Mineral Nutrition ◽  
Stress Responses ◽  
Foliar Application ◽  
Sucrose Concentration ◽  
Drought Stress Tolerance

Numerous studies have demonstrated the potential of sugar beet to lose the final sugar yield under water limiting regime. Ample evidences have revealed the important role of mineral nutrition in increasing plant tolerance to abiotic stresses. Despite the vital role of calcium (Ca2+) in plant growth and development, as well as in stress responses as an intracellular messenger, its role in alleviating drought stress in sugar beet has been rarely addressed. Here, an attempt was undertaken to investigate whether, and to what extent, foliar application of Ca2+ confers drought stress tolerance in sugar beet plants exposed to drought stress. To achieve this goal, sugar beet plants, which were grown in a high throughput phenotyping platform, were sprayed with Ca2+ and submitted to drought stress. The results showed that foliar application of Ca2+ increased the level of magnesium and silicon in the leaves, promoted plant growth, height, and leaf coverage area as well as chlorophyll level. Ca2+, in turn, increased the carbohydrate levels in leaves under drought condition and regulated transcriptionally the genes involved in sucrose transport (BvSUC3 and BvTST3). Subsequently, Ca2+ enhanced the root biomass and simultaneously led to induction of root (BvSUC3 and BvTST1) sucrose transporters which eventually supported the loading of more sucrose into beetroot under drought stress. Metabolite analysis revealed that the beneficial effect of Ca2+ in tolerance to drought induced-oxidative stress is most likely mediated by higher glutathione pools, increased levels of free polyamine putrescine (Put), and lower levels of amino acid gamma-aminobutyric acid (GABA). Taken together, this work demonstrates that foliar application of Ca2+ is a promising fertilization strategy to improve mineral nutrition efficiency, sugar metabolism, redox state, and thus, drought stress tolerance.

Production of betaine from Australian Melaleuca spp. for use in agriculture to reduce plant stress

2003 ◽  
Vol 43 (9) ◽  
pp. 1163 ◽  
Author(s):  
B. P. Naidu
Keyword(s):  
Stress Tolerance ◽  
Foliar Application ◽  
New South ◽  
Plant Stress ◽  
Positive Role ◽  
Tea Tree ◽  
South Wales ◽  
Simple Extraction ◽  
New Industry ◽  
Better Than

Some of the Melaleuca spp., native to Australia, are unique in their ability to withstand environmental stresses. The stress tolerance of these species is attributable to their ability to accumulate large quantities of organic compounds known as osmoprotectants or proline (betaine) analogues. Osmoprotectants can be extracted easily from these plants and used in seed treatment and foliar application to increase the stress tolerance of economic crops. This paper examines the potential of 8 Melaleuca spp. for the production of osmoprotectants. Melaleuca bracteata, which accumulates the proline analogue trans 4-hydroxy-N-methyl proline (MHP), was the most vigorous of all 8 species field tested in New South Wales and Queensland. A simple extraction protocol for commercial use is suggested. By growing M. bracteata, it is possible to achieve an average yield of 493 kg/ha of MHP along with 218 kg/ha of essential oil, with a gross economic return of AU$14505/ha. This return is better than that estimated for M. alternifolia, $3200/ha, which is currently grown for the production of tea tree oil. The cultivation of M. bracteata has the potential of creating a new industry for Australia, in addition to its positive role in the control of dryland salinity.

scholarly journals Molecular Programming of Drought-Challenged Trichoderma harzianum-Bioprimed Rice (Oryza sativa L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Bishnu Maya Bashyal ◽  
Pooja Parmar ◽  
Najam Waris Zaidi ◽  
Rashmi Aggarwal
Keyword(s):  
Drought Stress ◽  
Secondary Metabolites ◽  
Trichoderma Harzianum ◽  
Oryza Sativa L ◽  
Enrichment Analysis ◽  
Rice Cultivar ◽  
Glutathione Metabolism ◽  
Carbohydrate Binding ◽  
Molecular Programming ◽  
Phenyl Propanoid

Trichoderma biopriming enhances rice growth in drought-stressed soils by triggering various plant metabolic pathways related to antioxidative defense, secondary metabolites, and hormonal upregulation. In the present study, transcriptomic analysis of rice cultivar IR64 bioprimed with Trichoderma harzianum under drought stress was carried out in comparison with drought-stressed samples using next-generation sequencing techniques. Out of the 2,506 significant (p < 0.05) differentially expressed genes (DEGs), 337 (15%) were exclusively expressed in drought-stressed plants, 382 (15%) were expressed in T. harzianum-treated drought-stressed plants, and 1,787 (70%) were commonly expressed. Furthermore, comparative analysis of upregulated and downregulated genes under stressed conditions showed that 1,053 genes (42%) were upregulated and 733 genes (29%) were downregulated in T. harzianum-treated drought-stressed rice plants. The genes exclusively expressed in T. harzianum-treated drought-stressed plants were mostly photosynthetic and antioxidative such as plastocyanin, small chain of Rubisco, PSI subunit Q, PSII subunit PSBY, osmoproteins, proline-rich protein, aquaporins, stress-enhanced proteins, and chaperonins. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis states that the most enriched pathways were metabolic (38%) followed by pathways involved in the synthesis of secondary metabolites (25%), carbon metabolism (6%), phenyl propanoid (7%), and glutathione metabolism (3%). Some of the genes were selected for validation using real-time PCR which showed consistent expression as RNA-Seq data. Furthermore, to establish host–T. harzianum interaction, transcriptome analysis of Trichoderma was also carried out. The Gene Ontology (GO) analysis of T. harzianum transcriptome suggested that the annotated genes are functionally related to carbohydrate binding module, glycoside hydrolase, GMC oxidoreductase, and trehalase and were mainly upregulated, playing an important role in establishing the mycelia colonization of rice roots and its growth. Overall, it can be concluded that T. harzianum biopriming delays drought stress in rice cultivars by a multitude of molecular programming.

scholarly journals ScDREB10, an A-5c type of DREB Gene of the Desert Moss Syntrichia caninervis, Confers Osmotic and Salt Tolerances to Arabidopsis

Genes ◽  
2019 ◽  
Vol 10 (2) ◽  
pp. 146 ◽  
Author(s):  
Xiaoshuang Li ◽  
Yuqing Liang ◽  
Bei Gao ◽  
Meiheriguli Mijiti ◽  
Tohir A. Bozorov ◽  
...  
Keyword(s):  
Stress Tolerance ◽  
Plant Stress ◽  
Crop Productivity ◽  
Germination Percentage ◽  
Ros Scavenging ◽  
Transient Expression Assay ◽  
Syntrichia Caninervis ◽  
Stress Related Genes ◽  
Major Factors ◽  
Dreb Gene

Drought and salinity are major factors limiting crop productivity worldwide. DREB (dehydration-responsive element-binding) transcription factors play important roles in plant stress response and have been identified in a wide variety of plants. Studies on DREB are focused on the A-1 (DREB1) and A-2 (DREB2) groups. Studies on A-5 group DREBs, which represent a large proportion of the DREB subfamily, is limited. In this study, we characterized and analyzed the stress tolerance function of ScDREB10, an A-5c type DREB gene from the desert moss Syntrichia caninervis. Transactivation assay in yeast showed that ScDREB10 had transactivation activity. Transient expression assay revealed that ScDREB10 was distributed both in the nucleus and cytosol of tobacco leaf epidermal cells. Overexpression of ScDREB10 significantly increased the germination percentage of Arabidopsis seeds under osmotic and salt stresses, and improved the osmotic and salt stress tolerances of Arabidopsis at the seedling stage and is associated with the expression of downstream stress-related genes and improved reactive oxygen species (ROS) scavenging ability. Our study provides insight into the molecular mechanism of stress tolerance of A-5 type DREB proteins, as well as providing a promising candidate gene for crop salt and drought stress breeding.

Physiological and Biochemical Responses of Rice (Oryza Sativa L.) Varieties Against Drought Stress

2021 ◽  
Vol 50 (2) ◽  
pp. 335-342
Author(s):  
Ibrahim Selçuk Kuru ◽  
ÇIğdem Işikalan ◽  
Filiz Akbaş
Keyword(s):  
Oryza Sativa ◽  
Drought Stress ◽  
Stress Tolerance ◽  
Oryza Sativa L ◽  
Drought Treatment ◽  
Local Varieties ◽  
Drought Stress Tolerance ◽  
Biochemical Responses ◽  
Physiological And Biochemical

Physiological and biochemical responses of six different rice (Oryza sativa L.) varieties cultivated in Turkey and two local varieties, namely Karacadağ and Hazro to the drought were investigated. After 12 days of drought treatment, the highest and lowest OP values were observed in Osmancık-97 (-1.14 MPa) and Karacadağ (-1.55 MPa) varieties, respectively. In the same treatment, it was observed that the amount of proline increased 19.9-fold in Karacadağ and 3.6-fold in Osmancık-97. When the data obtained from all parameters were correlated with drought stress tolerance, Osmancık-97 and Beşer varieties were considered to be tolerant, while Gönen and Karacadağ varieties are considered to be more sensitive. Bangladesh J. Bot. 50(2): 335-342, 2021 (June)

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