scholarly journals Simultaneous Dual-Modal Multispectral Photoacoustic and Ultrasound Macroscopy for Three-Dimensional Whole-Body Imaging of Small Animals

Photonics ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 13
Author(s):  
Eun-Yeong Park ◽  
Sinyoung Park ◽  
Haeni Lee ◽  
Munsik Kang ◽  
Chulhong Kim ◽  
...  
Keyword(s):  
Ultrasound Imaging ◽  
Biological Tissue ◽  
Structural Information ◽  
Imaging Technique ◽  
Photoacoustic Imaging ◽  
Biological Tissues ◽  
Whole Body ◽  
Depth Information ◽  
Whole Body Imaging ◽  
Ultrasound Images

Photoacoustic imaging is a promising medical imaging technique that provides excellent function imaging of an underlying biological tissue or organ. However, it is limited in providing structural information compared to other imaging modalities, such as ultrasound imaging. Thus, to offer complete morphological details of biological tissues, photoacoustic imaging is typically integrated with ultrasound imaging. This dual-modal imaging technique is already implemented on commercial clinical ultrasound imaging platforms. However, commercial platforms suffer from limited elevation resolution compared to the lateral and axial resolution. We have successfully developed a dual-modal photoacoustic and ultrasound imaging to address these limitations, specifically targeting animal studies. The system can acquire whole-body images of mice in vivo and provide complementary structural and functional information of biological tissue information simultaneously. The color-coded depth information can be readily obtained in photoacoustic images using complementary information from ultrasound images. The system can be used for several biomedical applications, including drug delivery, biodistribution assessment, and agent testing.

Ultrasound Imaging Characterization of Soft Tissue Dynamics of the Seated Human Body

2020 ◽  
Vol 142 (6) ◽  
Author(s):  
Daisuke Yamada ◽  
Alperen Değirmenci ◽  
Robert D. Howe
Keyword(s):  
Ultrasound Imaging ◽  
Human Body ◽  
Human Subjects ◽  
Excitation Amplitude ◽  
Whole Body ◽  
Ultrasound Images ◽  
Body Dynamics ◽  
Excitation Pattern

Abstract To characterize the dynamics of internal soft organs and external anatomical structures, this paper presents a system that combines medical ultrasound imaging with an optical tracker and a vertical exciter that imparts whole-body vibrations on seated subjects. The spatial and temporal accuracy of the system was validated using a phantom with calibrated internal structures, resulting in 0.224 mm maximum root-mean-square (r.m.s.) position error and 13 ms maximum synchronization error between sensors. In addition to the dynamics of the head and sternum, stomach dynamics were characterized by extracting the centroid of the stomach from the ultrasound images. The system was used to characterize the subject-specific body dynamics as well as the intrasubject variabilities caused by excitation pattern (frequency up-sweep, down-sweep, and white noise, 1–10 Hz), excitation amplitude (1 and 2 m/s2 r.m.s.), seat compliance (rigid and soft), and stomach filling (empty and 500 mL water). Human subjects experiments (n = 3) yielded preliminary results for the frequency response of the head, sternum, and stomach. The method presented here provides the first detailed in vivo characterization of internal and external human body dynamics. Tissue dynamics characterized by the system can inform design of vehicle structures and adaptive control of seat and suspension systems, as well as validate finite element models for predicting passenger comfort in the early stages of vehicle design.

scholarly journals Characterization of a Fiber Bundle-Based Real-Time Ultrasound/Photoacoustic Imaging System and Its In Vivo Functional Imaging Applications

Micromachines ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 820
Author(s):  
He Leng ◽  
Yuhling Wang ◽  
De-Fu Jhang ◽  
Tsung-Sheng Chu ◽  
Chia-Hui Tsao ◽  
...  
Keyword(s):  
User Interface ◽  
Real Time ◽  
In Vivo Imaging ◽  
Fiber Bundle ◽  
Imaging System ◽  
Structural Information ◽  
Photoacoustic Imaging ◽  
Numerical Aperture ◽  
Biological Tissues

Photoacoustic (PA) imaging is an attractive technology for imaging biological tissues because it can capture both functional and structural information with satisfactory spatial resolution. Current commercially available PA imaging systems are limited by their bulky size or inflexible user interface. We present a new handheld real-time ultrasound/photoacoustic imaging system (HARP) consisting of a detachable, high-numerical-aperture (NA) fiber bundle-based illumination system integrated with an array-based ultrasound (US) transducer and a data acquisition platform. In this system, different PA probes can be used for different imaging applications by switching the transducers and the corresponding jackets to combine the fiber pads and transducer into a single probe. The intuitive user interface is a completely programmable MATLAB-based platform. In vitro phantom experiments were conducted to test the imaging performance of the developed PA system. Furthermore, we demonstrated (1) in vivo brain vasculature imaging, (2) in vivo imaging of real-time stimulus-evoked cortical hemodynamic changes during forepaw electrical stimulation, and (3) in vivo imaging of real-time cerebral pharmacokinetics in rats using the developed PA system. The overall purpose of this design concept for a customizable US/PA imaging system is to help overcome the diverse challenges faced by medical researchers performing both preclinical and clinical PA studies.

scholarly journals Ultrasound-on-chip platform for medical imaging, analysis, and collective intelligence

2021 ◽  
Vol 118 (27) ◽  
pp. e2019339118
Author(s):  
Jonathan M. Rothberg ◽  
Tyler S. Ralston ◽  
Alex G. Rothberg ◽  
John Martin ◽  
Jaime S. Zahorian ◽  
...  
Keyword(s):  
Ultrasound Imaging ◽  
Microelectromechanical Systems ◽  
Collective Intelligence ◽  
Low Cost ◽  
The Body ◽  
Oxide Semiconductor ◽  
Whole Body ◽  
Whole Body Imaging ◽  
Diagnostic Quality ◽  
On Chip

Over the past half-century, ultrasound imaging has become a key technology for assessing an ever-widening range of medical conditions at all stages of life. Despite ultrasound’s proven value, expensive systems that require domain expertise in image acquisition and interpretation have limited its broad adoption. The proliferation of portable and low-cost ultrasound imaging can improve global health and also enable broad clinical and academic studies with great impact on the fields of medicine. Here, we describe the design of a complete ultrasound-on-chip, the first to be cleared by the Food and Drug Administration for 13 indications, comprising a two-dimensional array of silicon-based microelectromechanical systems (MEMS) ultrasonic sensors directly integrated into complementary metal–oxide–semiconductor-based control and processing electronics to enable an inexpensive whole-body imaging probe. The fabrication and design of the transducer array with on-chip analog and digital circuits, having an operating power consumption of 3 W or less, are described, in which approximately 9,000 seven-level feedback-based pulsers are individually addressable to each MEMS element and more than 11,000 amplifiers, more than 1,100 analog-to-digital converters, and more than 1 trillion operations per second are implemented. We quantify the measured performance and the ability to image areas of the body that traditionally takes three separate probes. Additionally, two applications of this platform are described—augmented reality assistance that guides the user in the acquisition of diagnostic-quality images of the heart and algorithms that automate the measurement of cardiac ejection fraction, an indicator of heart health.

scholarly journals Generation and Analysis of Ultrasound Images Using Plane Wave and Sparse Arrays Techniques

Sensors ◽  
2018 ◽  
Vol 18 (11) ◽  
pp. 3660 ◽  
Author(s):  
Nivaldo Schiefler ◽  
Joaquim Maia ◽  
Fabio Schneider ◽  
Acácio Zimbico ◽  
Amauri Assef ◽  
...  
Keyword(s):  
Plane Wave ◽  
Ultrasound Imaging ◽  
Linear Array ◽  
Imaging Technique ◽  
Simulated Data ◽  
Ultrasound Images ◽  
Sparse Arrays ◽  
Array Transducer ◽  
Processing And Storage ◽  
And Storage

Ultrasonic imaging is one of the most important techniques to help medical diagnosis. However, obtaining high quality images requires the acquisition, processing, and storage of a large amount of data. In this work, we evaluated a new ultrasound imaging technique based on plane wave and sparse arrays to increase the scan rate and reduce the amount of data amount to be stored. The performance of the proposed method was tested using simulated echo data (from Field II) and phantom data acquired using a Verasonics system equipped with a L11-4v linear array transducer. The tests were done using 128 elements for transmission and 128, 65, 44, and 23 elements sparsely distributed for reception. The simulated data were compared with images obtained with the Delay and Sum (DAS) method and the experimental data were compared with those acquired from Verasonics. The obtained results using the Full Width at Half Maximum (FWHM) criteria at −6 dB showed that the images generated by the proposed method were similar in terms of resolutions (axial and lateral) and contrast to the simulated and the Verasonics commercial ones, indicating that the sparse reception proposed method is suitable for ultrasound imaging.

Characterization of a whole body imaging technique for PET

1990 ◽  
Vol 37 (2) ◽  
pp. 676-680 ◽  
Author(s):  
T.M. Guerrero ◽  
E.J. Hoffman ◽  
M. Dahlbom ◽  
P.D. Cutler ◽  
R.A. Hawkins ◽  
...  
Keyword(s):  
Imaging Technique ◽  
Whole Body ◽  
Whole Body Imaging ◽  
Body Imaging

scholarly journals An Adjustable Dark-Field Acoustic-Resolution Photoacoustic Imaging System with Fiber Bundle-Based Illumination

Biosensors ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 262
Author(s):  
Yuhling Wang ◽  
De-Fu Jhang ◽  
Tsung-Sheng Chu ◽  
Chia-Hui Tsao ◽  
Chia-Hua Tsai ◽  
...  
Keyword(s):  
Real Time ◽  
Imaging System ◽  
Structural Information ◽  
Photoacoustic Imaging ◽  
Biological Tissues ◽  
Dark Field ◽  
Design Concept ◽  
High Frequency Ultrasound

Photoacoustic (PA) imaging has become one of the major imaging methods because of its ability to record structural information and its high spatial resolution in biological tissues. Current commercialized PA imaging instruments are limited to varying degrees by their bulky size (i.e., the laser or scanning stage) or their use of complex optical components for light delivery. Here, we present a robust acoustic-resolution PA imaging system that consists of four adjustable optical fibers placed 90° apart around a 50 MHz high-frequency ultrasound (US) transducer. In the compact design concept of the PA probe, the relative illumination parameters (i.e., angles and fiber size) can be adjusted to fit different imaging applications in a single setting. Moreover, this design concept involves a user interface built in MATLAB. We first assessed the performance of our imaging system using in vitro phantom experiments. We further demonstrated the in vivo performance of the developed system in imaging (1) rat ear vasculature, (2) real-time cortical hemodynamic changes in the superior sagittal sinus (SSS) during left-forepaw electrical stimulation, and (3) real-time cerebral indocyanine green (ICG) dynamics in rats. Collectively, this alignment-free design concept of a compact PA probe without bulky optical lens systems is intended to satisfy the diverse needs in preclinical PA imaging studies.

Automated electron tomography: from data collection to image processing

1995 ◽  
Vol 53 ◽  
pp. 26-27
Author(s):  
Weiping Liu ◽  
Jennifer Fung ◽  
W.J. de Ruijter ◽  
Hans Chen ◽  
John W. Sedat ◽  
...  
Keyword(s):  
Image Processing ◽  
Data Collection ◽  
Structural Information ◽  
Imaging Technique ◽  
Electron Tomography ◽  
Ccd Camera ◽  
Automated Data Collection ◽  
Full Control ◽  
Transmission Electron ◽  
Amorphous Structures

Electron tomography is a technique where many projections of an object are collected from the transmission electron microscope (TEM), and are then used to reconstruct the object in its entirety, allowing internal structure to be viewed. As vital as is the 3-D structural information and with no other 3-D imaging technique to compete in its resolution range, electron tomography of amorphous structures has been exercised only sporadically over the last ten years. Its general lack of popularity can be attributed to the tediousness of the entire process starting from the data collection, image processing for reconstruction, and extending to the 3-D image analysis. We have been investing effort to automate all aspects of electron tomography. Our systems of data collection and tomographic image processing will be briefly described.To date, we have developed a second generation automated data collection system based on an SGI workstation (Fig. 1) (The previous version used a micro VAX). The computer takes full control of the microscope operations with its graphical menu driven environment. This is made possible by the direct digital recording of images using the CCD camera.

Applications of Time-OF-Flight Secondary Ion Mass Spectrometry (TOF-SIMS)

1990 ◽  
Vol 48 (2) ◽  
pp. 308-309
Author(s):  
Bruno Schueler ◽  
Robert W. Odom
Keyword(s):  
Mass Spectrometry ◽  
Secondary Ion Mass Spectrometry ◽  
Structural Information ◽  
Time Of Flight ◽  
Biological Tissues ◽  
Polymer Surfaces ◽  
Tof Sims ◽  
Secondary Ions ◽  
Ion Mass Spectrometry ◽  
Secondary Ion

Time-of-flight secondary ion mass spectrometry (TOF-SIMS) provides unique capabilities for elemental and molecular compositional analysis of a wide variety of surfaces. This relatively new technique is finding increasing applications in analyses concerned with determining the chemical composition of various polymer surfaces, identifying the composition of organic and inorganic residues on surfaces and the localization of molecular or structurally significant secondary ions signals from biological tissues. TOF-SIMS analyses are typically performed under low primary ion dose (static SIMS) conditions and hence the secondary ions formed often contain significant structural information.This paper will present an overview of current TOF-SIMS instrumentation with particular emphasis on the stigmatic imaging ion microscope developed in the authors’ laboratory. This discussion will be followed by a presentation of several useful applications of the technique for the characterization of polymer surfaces and biological tissues specimens. Particular attention in these applications will focus on how the analytical problem impacts the performance requirements of the mass spectrometer and vice-versa.

EMCAT: An automatic image-processing system for electron-microscopic tomography

1994 ◽  
Vol 52 ◽  
pp. 418-419
Author(s):  
Weiping Liu ◽  
John W. Sedat ◽  
David A. Agard
Keyword(s):  
Image Processing ◽  
Structural Information ◽  
Imaging Technique ◽  
Three Dimensional ◽  
Processing System ◽  
Electron Microscopic ◽  
Data Set ◽  
Ordered Structures ◽  
Automatic Image Processing ◽  
Em Tomography

Any real world object is three-dimensional. The principle of tomography, which reconstructs the 3-D structure of an object from its 2-D projections of different view angles has found application in many disciplines. Electron Microscopic (EM) tomography on non-ordered structures (e.g., subcellular structures in biology and non-crystalline structures in material science) has been exercised sporadically in the last twenty years or so. As vital as is the 3-D structural information and with no existing alternative 3-D imaging technique to compete in its high resolution range, the technique to date remains the kingdom of a brave few. Its tedious tasks have been preventing it from being a routine tool. One keyword in promoting its popularity is automation: The data collection has been automated in our lab, which can routinely yield a data set of over 100 projections in the matter of a few hours. Now the image processing part is also automated. Such automations finish the job easier, faster and better.

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