scholarly journals The Antistaphylococcal Activity of Citropin 1.1 and Temporin A against Planktonic Cells and Biofilms Formed by Isolates from Patients with Atopic Dermatitis: An Assessment of Their Potential to Induce Microbial Resistance Compared to Conventional Antimicrobials

2016 ◽  
Vol 9 (2) ◽  
pp. 30 ◽  
Author(s):  
Malgorzata Dawgul ◽  
Wioletta Baranska-Rybak ◽  
Lidia Piechowicz ◽  
Marta Bauer ◽  
Damian Neubauer ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Planktonic Cells ◽  
Microbial Resistance ◽  
Antistaphylococcal Activity ◽  
Citropin 1.1 ◽  
Temporin A

scholarly journals Activity of Temporin A and Short Lipopeptides Combined with Gentamicin against Biofilm Formed by Staphylococcus aureus and Pseudomonas aeruginosa

Antibiotics ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 566 ◽  
Author(s):  
Malgorzata Anna Paduszynska ◽  
Katarzyna Ewa Greber ◽  
Wojciech Paduszynski ◽  
Wieslaw Sawicki ◽  
Wojciech Kamysz
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Solid Phase ◽  
Well Being ◽  
Bacterial Biofilm ◽  
Planktonic Cells ◽  
Strong Activity ◽  
Phase Temperature ◽  
Synthesis Methodology ◽  
Temporin A

The formation of biofilms on biomaterials causes biofilm-associated infections. Available treatments often fail to fight the microorganisms in the biofilm, creating serious risks for patient well-being and life. Due to their significant antibiofilm activities, antimicrobial peptides are being intensively investigated in this regard. A promising approach is a combination therapy that aims to increase the efficacy and broaden the spectrum of antibiotics. The main goal of this study was to evaluate the antimicrobial efficacy of temporin A and the short lipopeptides (C10)2-KKKK-NH2 and (C12)2-KKKK-NH2 in combination with gentamicin against biofilm formed by Staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA). Peptides were synthesized with solid-phase temperature-assisted synthesis methodology. The minimum inhibitory concentrations (MICs), fractional inhibitory concentrations (FICs), minimum biofilm eradication concentrations (MBECs), and the influence of combinations of compounds with gentamicin on bacterial biofilm were determined for reference strains of SA (ATCC 25923) and PA (ATCC 9027). The peptides exhibited significant potential to enhance the antibacterial activity of gentamicin against SA biofilm, but there was no synergy in activity against planktonic cells. The antibiotic applied alone demonstrated strong activity against planktonic cells and poor effectiveness against SA biofilm. Biofilm formed by PA was much more sensitive to gentamicin, but some positive influences of supplementation with peptides were noticed. The results of the performed experiments suggest that the potential application of peptides as adjuvant agents in the treatment of biofilm-associated infections should be studied further.

scholarly journals A Novel Delivery System for the Controlled Release~of Antimicrobial Peptides: Citropin 1.1 and Temporin A

Polymers ◽  
2018 ◽  
Vol 10 (5) ◽  
pp. 489 ◽  
Author(s):  
Urszula Piotrowska ◽  
Ewa Oledzka ◽  
Anna Zgadzaj ◽  
Marta Bauer ◽  
Marcin Sobczak
Keyword(s):  
Controlled Release ◽  
Antimicrobial Peptides ◽  
Delivery System ◽  
Citropin 1.1 ◽  
Temporin A

scholarly journals Activity of Antimicrobial Peptides and Conventional Antibiotics against Superantigen Positive Staphylococcus aureus Isolated from the Patients with Neoplastic and Inflammatory Erythrodermia

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Wioletta Baranska-Rybak ◽  
Oscar Cirioni ◽  
Malgorzata Dawgul ◽  
Malgorzata Sokolowska-Wojdylo ◽  
Lukasz Naumiuk ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Peptides ◽  
Therapeutic Implication ◽  
Human Diseases ◽  
High Efficacy ◽  
Opposite Situation ◽  
Conventional Antibiotics ◽  
Aurein 1.2 ◽  
Citropin 1.1 ◽  
Temporin A

Superantigens are proteins comprising a group of molecules produced by various microorganisms. They are involved in pathogenesis of several human diseases. The aim of the study was the comparison of susceptibility to antibiotics and antimicrobial peptides (AMPs) of Staphylococcus aureus (SA) strains producing staphylococcal enterotoxins SEA, SEB, SEC, SED, and TSST-1 and nonproducing ones. In the group of the total 28 of the patients with erythrodermia the presence of SA was confirmed in 24 cases. The total of 14 strains of SA excreted enterotoxins SEA, SEC, SED, and TSST-1. We did not observe that strains producing mentioned superantigens were less susceptible to AMPs (aurein 1.2, citropin 1.1, lipopeptide, protegrin 1, tachyplesin 3, temporin A, and uperin 3.6). The opposite situation was observed in conventional antibiotics. SA strains excreting tested superantigens had higher MICs and MBCs than nonproducing ones. The interesting finding considering the high efficacy of AMPs, against all examined strains of SA, makes them attractive candidates for therapeutic implication.

Antimicrobial Activity of Different Antimicrobial Peptides (AMPs) Against Clinical Methicillin-resistant Staphylococcus aureus (MRSA)

2019 ◽  
Vol 18 (24) ◽  
pp. 2116-2126 ◽  
Author(s):  
Eleonora Ciandrini ◽  
Gianluca Morroni ◽  
Daniela Arzeni ◽  
Wojciech Kamysz ◽  
Damian Neubauer ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antimicrobial Peptides ◽  
Growth Inhibition ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Clinical Isolates ◽  
Methicillin Resistant ◽  
Time Kill ◽  
Citropin 1.1 ◽  
Temporin A

Background: Antimicrobial research is being focused to look for more effective therapeutics against antibiotic-resistant infections caused by methicillin-resistant Staphylococcus aureus (MRSA). In this direction, antimicrobial peptides (AMP) appear as promising tool. Objectives: This study evaluated the antimicrobial activity of different AMPs (Citropin 1.1, Temporin A, Pexiganan, CA(1–7)M(2–9)NH2, Pal-KGK-NH2, Pal-KKKK-NH2, LL-37) against human MRSA clinical isolates. Methods: The Minimum Inhibitory Concentration (MIC) was assessed for each AMP; then, the most active ones (Citropin 1.1, Temporin A, CA(1–7)M(2–9)NH2 and Pal-KGK-NH2) were tested against selected MRSA strains by time-kill studies. Results: The lowest MIC value was observed for Pal-KGK-NH2 (1 µg/ml), followed by Temporin A (4- 16 µg/ml), CA(1–7)M(2–9)NH2 (8-16 µg/ml) and Citropin 1.1 (16-64 µg/ml), while higher MICs were evidenced for LL-37, Pexiganan and Pal-KKKK-NH2 (> 128 µg/ml). In time-kill experiments, Citropin 1.1 and CA(1-7)M(2-9)NH2 showed a relatively high percentage of growth inhibition (>30 %) for all the tested MRSA clinical isolates, with a dose-dependent activity resulting in the highest percentage of bacterial growth inhibition (89.39%) at 2MIC concentration. Conclusion: Overall, our data demonstrated the potential of some AMPs against MRSA isolates, such as Citropin 1.1 and CA(1-7)M(2-9)NH2, that represents a promising area of development for different clinical applications.

scholarly journals Synthetic Amphibian Peptides and Short Amino-Acids Derivatives against Planktonic Cells and Mature Biofilm of Providencia stuartii Clinical Strains

2014 ◽  
Vol 63 (4) ◽  
pp. 423-431
Author(s):  
KINGA OSTROWSKA ◽  
WOJCIECH KAMYSZ ◽  
MAŁGORZATA DAWGUL ◽  
ANTONI RÓŻALSKI
Keyword(s):  
Amino Acids ◽  
Biofilm Formation ◽  
Synthetic Peptides ◽  
Antimicrobial Agents ◽  
Multidrug Resistant ◽  
Planktonic Cells ◽  
Providencia Stuartii ◽  
Conventional Antibiotics ◽  
Susceptibility Assay ◽  
Citropin 1.1

Over the last decade, the growing number of multidrug resistant strains limits the use of many of the currently available chemotherapeutic agents. Furthermore, bacterial biofilm, due to its complex structure, constitutes an effective barrier to conventional antibiotics. The in vitro activities of naturally occurring peptide (Citropin 1.1), chemically engineered analogue (Pexiganan), newly-designed, short amino-acid derivatives (Pal-KK-NH2, Pal-KKK-NH2, Pal-RRR-NH2) and six clinically used antimicrobial agents (Gatifloxacin, Ampicilin, Cefotaxime, Ceftriaxone, Cefuroxime and Cefalexin) were investigated against planktonic cells and mature biofilm of multidrug-resistant Providencia stuartii strains, isolated from urological catheters. The MICs, MBCs values were determined by broth microdilution technique. Inhibition of biofilm formation by antimicrobial agents as well as biofilm susceptibility assay were tested using a surrogate model based on the Crystal Violet method. The antimicrobial activity of amino-acids derivatives and synthetic peptides was compared to that of clinically used antibiotics. For planktonic cells, MICs of peptides and antibiotics ranged between 1 and 256 μg/ml and 256 and ≥ 2048 μg/ml, respectively. The MBCs values of Pexiganan, Citropin 1.1 and amino-acids derivatives were between 16 and 256 μg/ml, 64 and 256 μg/ml and 16 and 512 μg/ml, respectively. For clinically used antibiotics the MBCs values were above 2048 μg/ml. All of the tested peptides and amino-acids derivatives, showed inhibitory activity against P. stuartii biofilm formation, in relation to their concentrations. Pexiganan and Citropin 1.1 in concentration range 32 and 256 μg/ml caused both strong and complete suppression of biofilm formation. None of the antibiotics caused complete inhibition of biofilm formation process. The biofilm susceptibility assay verified the extremely poor antibiofilm activity of conventional antibiotics compared to synthetic peptides. The obtained results showed that synthetic peptides are generally more potent and effective than clinically used antibiotics.

Diet and heredity in infantile atopic dermatitis

1972 ◽  
Vol 105 (3) ◽  
pp. 400-404 ◽  
Author(s):  
H. S. Kaufman
Keyword(s):  
Atopic Dermatitis
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