scholarly journals Evaluation of Gallic Acid-Coated Gold Nanoparticles as an Anti-Aging Ingredient

2021 ◽  
Vol 14 (11) ◽  
pp. 1071
Author(s):  
Yun-Zhen Wu ◽  
Yen-Yu Tsai ◽  
Long-Sen Chang ◽  
Ying-Jung Chen
Keyword(s):  
Gold Nanoparticles ◽  
Gallic Acid ◽  
High Glucose ◽  
Type I Collagen ◽  
Dermal Fibroblast ◽  
Skin Aging ◽  
Hydrodynamic Diameter ◽  
Type I ◽  
Matrix Metalloproteinase 1 ◽  
Transmission Electron

Hyperglycemic environment-induced oxidative stress-mediated matrix metalloproteinase-1 (MMP-1) plays a crucial role in the degradation of the extracellular matrix (ECM), which might contribute to premature skin aging. Synthesized, environmentally friendly gallic acid-coated gold nanoparticles (GA–AuNPs) have been evaluated as an anti-aging antioxidant. Their microstructure was characterized by transmission electron microscopy (TEM), which showed that GA–AuNPs are spherical when prepared at pH 11. Dynamic light scattering (DLS) analysis revealed that the average hydrodynamic diameter of a GA–AuNP is approximately 40 nm and with a zeta potential of −49.63 ± 2.11 mV. Additionally, the present data showed that GA–AuNPs have a superior ability to inhibit high glucose-mediated MMP-1-elicited type I collagen degradation in dermal fibroblast cells. Collectively, our data indicated that high-glucose-mediated ROS production was reduced upon cell treatment with GA–AuNPs, which blocked p38 MAPK/ERK-mediated c-Jun, c-Fos, ATF-2 phosphorylation, and the phosphorylation of NFκB, leading to the down-regulation of MMP-1 mRNA and protein expression in high glucose-treated cells. Our findings suggest that GA-AuNPs have a superior ability to inhibit high-glucose-mediated MMP-1-elicited ECM degradation, which highlights its potential as an anti-aging ingredient.

scholarly journals Erjingwan Extracts Exert Antiaging Effects of Skin through Activating Nrf2 and Inhibiting NF-κB

2019 ◽  
Vol 2019 ◽  
pp. 1-15
Author(s):  
Hairong Zhong ◽  
Choyoung Hong ◽  
Zhouxin Han ◽  
Seung Jin Hwang ◽  
Byunghyun Kim ◽  
...  
Keyword(s):  
Type I Collagen ◽  
Radical Scavenging ◽  
Skin Aging ◽  
Type I ◽  
Healthy Human ◽  
Noninvasive Measurements ◽  
Matrix Metalloproteinase 1 ◽  
Nourishing Yin ◽  
Medical Plants

In oriental medicine, mixtures of medical plants are always used as prescriptions for diseases. Natural products extracted from herbs have great potential antiaging effects. Previous studies and clinical trials have shown several critical functions of Erjingwan (EJW), such as nourishing Yin, kidney tonifying and aging-resistance. We assumed that EJW extracts exerted the antiaging effects through nourishing Yin. We examined the antiaging effects of EJW extracts on healthy human skin by noninvasive measurements. Then we estimated the cell proliferation and DPPH radical scavenging rate. Western blotting analysis was used to determine the expressions of matrix metalloproteinase-1 (MMP-1), type I collagen (COL1A2), p-NF-κB, NF-κB, p-IκBα, IκBα, p-Nrf2, and HO-1. EJW extracts did not affect moisture content, TEWL and skin chroma, while it significantly improved skin glossiness and skin elasticity. Moreover, EJW extracts could downregulate the MMP1 expression and upregulate the COL1A2 expression. In addition, it promoted the Nrf2 pathway while it inhibited the NF-κB pathway. With the application of cream containing EJW extracts, the skin aging state was significantly improved. Furthermore, in vitro studies showed that EJW extracts contributed to the repair of skin after injury. Taken together, the antiaging effects of EJW extracts were related to its antioxidant and anti-inflammatory abilities.

scholarly journals Effects of Tenascin C on the Integrity of Extracellular Matrix and Skin Aging

2020 ◽  
Vol 21 (22) ◽  
pp. 8693
Author(s):  
Young Eun Choi ◽  
Min Ji Song ◽  
Mari Hara ◽  
Kyoko Imanaka-Yoshida ◽  
Dong Hun Lee ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Type I Collagen ◽  
Transforming Growth Factor ◽  
Mrna Level ◽  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Cell Physiology ◽  
Type I ◽  
Tenascin C ◽  
Matrix Metalloproteinase 1

Tenascin C (TNC) is an element of the extracellular matrix (ECM) of various tissues, including the skin, and is involved in modulating ECM integrity and cell physiology. Although skin aging is apparently associated with changes in the ECM, little is known about the role of TNC in skin aging. In this study, we found that the Tnc mRNA level was significantly reduced in the skin tissues of aged mice compared with young mice, consistent with reduced TNC protein expression in aged human skin. TNC-large (TNC-L; 330-kDa) and -small (TNC-S; 240-kDa) polypeptides were observed in conditional media from primary dermal fibroblasts. Both recombinant TNC polypeptides, corresponding to TNC-L and TNC-S, increased the expression of type I collagen and reduced the expression of matrix metalloproteinase-1 in fibroblasts. Treatment of fibroblasts with a recombinant TNC polypeptide, corresponding to TNC-L, induced phosphorylation of SMAD2 and SMAD3. TNC increased the level of transforming growth factor-β1 (TGF-β1) mRNA and upregulated the expression of type I collagen by activating the TGF-β signaling pathway. In addition, TNC also promoted the expression of type I collagen in fibroblasts embedded in a three-dimensional collagen matrix. Our findings suggest that TNC contributes to the integrity of ECM in young skin and to prevention of skin aging.

scholarly journals Effects of Tenascin C on the Integrity of Extracellular Matrix and Skin Aging

2020 ◽  
Author(s):  
Young Eun Choi ◽  
Min Ji Song ◽  
Mari Hara ◽  
Kyoko Imanaka-Yoshida ◽  
Dong Hun Lee ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Type I Collagen ◽  
Mrna Level ◽  
Collagen Matrix ◽  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Cell Physiology ◽  
Type I ◽  
Tenascin C ◽  
Matrix Metalloproteinase 1

Tenascin C (TNC) is an element of the extracellular matrix (ECM) of various tissues, including the skin, and is involved in modulating ECM integrity and cell physiology. Although skin aging is apparently associated with changes in the ECM, little is known about the role of TNC in skin aging. Here we found that Tnc mRNA level was significantly reduced in the skin tissues of aged mice compared with young mice, consistent with reduced TNC protein expression in aged human skin. TNC-large (TNC-L; 330-kDa) and -small (TNC-S; 240-kDa) polypeptides were observed in conditional media from primary dermal fibroblasts. Both recombinant TNC polypeptides, corresponding to TNC-L and TNC-S, increased the expression of type I collagen and reduced the expression of matrix metalloproteinase-1 in fibroblasts. Treatment of fibroblasts with a recombinant TNC polypeptide, corresponding to TNC-L, induced phosphorylation of SMAD2 and SMAD3. TNC increased the level of TGF-β1 mRNA and upregulated the expression of type I collagen by activating the TGF-β signaling pathway. In addition, TNC also promoted the expression of type I collagen in fibroblasts embedded in a three-dimensional collagen matrix. Our findings suggest that TNC contributes to the integrity of ECM in young skin and to prevention of skin aging.

scholarly journals Acetylated Resveratrol and Oxyresveratrol Suppress UVB-Induced MMP-1 Expression in Human Dermal Fibroblasts

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1252
Author(s):  
Jae-Eun Lee ◽  
Jijeong Oh ◽  
Daeun Song ◽  
Mijeong Lee ◽  
Dongyup Hahn ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Type I Collagen ◽  
Dermal Fibroblast ◽  
Aging Effect ◽  
Inhibitory Effect ◽  
Human Dermal Fibroblast ◽  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Type I ◽  
Hdf Cells

Resveratrol (RES) and oxyresveratrol (OXYRES) are considered and utilized as active ingredients of anti-aging skin cosmetics. However, these compounds are susceptible to oxidative discoloration and unpleasant odor in solutions, limiting their use in cosmetics. Accordingly, RES and OXYRES were chemically modified to acetylated derivatives with enhanced stability, and their anti-aging effect on the skin and detailed molecular mechanism of their acetylated derivatives were investigated. Acetylated RES and OXYRES lost their acetyl group and exerted an inhibitory effect on H2O2-induced ROS levels in human dermal fibroblast (HDF) cells. In addition, RES, OXYRES, and their acetylated derivatives suppressed UVB-induced matrix metalloproteinase (MMP)-1 expression via inhibition of mitogen-activated protein kinases (MAPKs) and Akt/mTOR signaling pathways. Furthermore, RES, OXYRES, and their acetylated derivatives suppressed type I collagen in TPA-treated HDF cells. Collectively, these results suggest the beneficial effects and underlying molecular mechanisms of RES, OXYRES, and their acetylated derivatives for anti- skin aging applications.

scholarly journals Increased Fibroblast Metabolic Activity of Collagen Scaffolds via the Addition of Propolis Nanoparticles

Materials ◽  
2020 ◽  
Vol 13 (14) ◽  
pp. 3118
Author(s):  
Jeimmy González-Masís ◽  
Jorge M. Cubero-Sesin ◽  
Yendry R. Corrales-Ureña ◽  
Sara González-Camacho ◽  
Nohelia Mora-Ugalde ◽  
...  
Keyword(s):  
Metabolic Activity ◽  
Self Assembly ◽  
Type I Collagen ◽  
Fibroblast Cell ◽  
Antimicrobial Activities ◽  
Hydrodynamic Diameter ◽  
Type I ◽  
Collagen Scaffolds ◽  
Simple Method ◽  
Socially Relevant

Propolis natural extracts have been used since ancient times due to their antioxidant, anti-inflammatory, antiviral, and antimicrobial activities. In this study, we produced scaffolds of type I collagen, extracted from Wistar Hanover rat tail tendons, and impregnated them with propolis nanoparticles (NPs) for applications in regenerative medicine. Our results show that the impregnation of propolis NPs to collagen scaffolds affected the collagen denaturation temperature and tensile strength. The changes in structural collagen self-assembly due to contact with organic nanoparticles were shown for the first time. The fibril collagen secondary structure was preserved, and the D-pattern gap increased to 135 ± 28 nm, without losing the microfiber structure. We also show that the properties of the collagen scaffolds depended on the concentration of propolis NPs. A concentration of 100 μg/mL of propolis NPs with 1 mg of collagen, with a hydrodynamic diameter of 173 nm, was found to be an optimal concentration to enhance 3T3 fibroblast cell metabolic activity and cell proliferation. The expected outcome from this research is both scientifically and socially relevant since the home scaffold using natural nanoparticles can be produced using a simple method and could be widely used for local medical care in developing communities.

scholarly journals Skullcapflavone II Inhibits Degradation of Type I Collagen by Suppressing MMP-1 Transcription in Human Skin Fibroblasts

2019 ◽  
Vol 20 (11) ◽  
pp. 2734 ◽  
Author(s):  
Young Hun Lee ◽  
Eun Kyoung Seo ◽  
Seung-Taek Lee
Keyword(s):  
Type I Collagen ◽  
Three Dimensional ◽  
Transcriptional Level ◽  
Type I ◽  
Cancer Therapies ◽  
Dependent Manner ◽  
Reverse Transcription Pcr ◽  
Matrix Metalloproteinase 1 ◽  
Extracellular Signal ◽  
Tnf Α

Skullcapflavone II is a flavonoid derived from the root of Scutellaria baicalensis, a herbal medicine used for anti-inflammatory and anti-cancer therapies. We analyzed the effect of skullcapflavone II on the expression of matrix metalloproteinase-1 (MMP-1) and integrity of type I collagen in foreskin fibroblasts. Skullcapflavone II did not affect the secretion of type I collagen but reduced the secretion of MMP-1 in a dose- and time-dependent manner. Real-time reverse transcription-PCR and reporter gene assays showed that skullcapflavone II reduced MMP-1 expression at the transcriptional level. Skullcapflavone II inhibited the serum-induced activation of the extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) signaling pathways required for MMP-1 transactivation. Skullcapflavone II also reduced tumor necrosis factor (TNF)-α-induced nuclear factor kappa light chain enhancer of activated B cells (NF-κB) activation and subsequent MMP-1 expression. In three-dimensional culture of fibroblasts, skullcapflavone II down-regulated TNF-α-induced MMP-1 secretion and reduced breakdown of type I collagen. These results indicate that skullcapflavone II is a novel biomolecule that down-regulates MMP-1 expression in foreskin fibroblasts and therefore could be useful in therapies for maintaining the integrity of extracellular matrix.

scholarly journals A Novel Host/Tumor Cell Interaction Activates Matrix Metalloproteinase 1 and Mediates Invasion through Type I Collagen

1999 ◽  
Vol 274 (36) ◽  
pp. 25371-25378 ◽  
Author(s):  
Ulrike Benbow ◽  
Matthias P. Schoenermark ◽  
Teresa I. Mitchell ◽  
Joni L. Rutter ◽  
Ken-ichi Shimokawa ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Tumor Cell ◽  
Type I Collagen ◽  
Cell Interaction ◽  
Type I ◽  
Matrix Metalloproteinase 1 ◽  
Novel Host
Sign in / Sign up

Export Citation Format

Share Document