scholarly journals Formulations for Bacteriophage Therapy and the Potential Uses of Immobilization

2021 ◽  
Vol 14 (4) ◽  
pp. 359
Author(s):  
Daniel Rosner ◽  
Jason Clark
Keyword(s):  
Clinical Trials ◽  
Underlying Disease ◽  
Alternative Form ◽  
Bacterial Strains ◽  
Bacteriophage Therapy ◽  
Antibiotic Resistant ◽  
Bacterial Diseases ◽  
Future Success ◽  
Effective Delivery ◽  
Phage Selection

The emergence of antibiotic-resistant pathogens is becoming increasingly problematic in the treatment of bacterial diseases. This has led to bacteriophages receiving increased attention as an alternative form of treatment. Phages are effective at targeting and killing bacterial strains of interest and have yielded encouraging results when administered as part of a tailored treatment to severely ill patients as a last resort. Despite this, success in clinical trials has not always been as forthcoming, with several high-profile trials failing to demonstrate the efficacy of phage preparations in curing diseases of interest. Whilst this may be in part due to reasons surrounding poor phage selection and a lack of understanding of the underlying disease, there is growing consensus that future success in clinical trials will depend on effective delivery of phage therapeutics to the area of infection. This can be achieved using bacteriophage formulations instead of purely liquid preparations. Several encapsulation-based strategies can be applied to produce phage formulations and encouraging results have been observed with respect to efficacy as well as long term phage stability. Immobilization-based approaches have generally been neglected for the production of phage therapeutics but could also offer a viable alternative.

scholarly journals Bacteriophage therapy: experience from the Eliava Institute, Georgia

2008 ◽  
Vol 29 (2) ◽  
pp. 96 ◽  
Author(s):  
Nina Chanishvili ◽  
Richard Sharp
Keyword(s):  
Soviet Union ◽  
Former Soviet Union ◽  
Bacterial Infections ◽  
Phage Therapy ◽  
Effective Means ◽  
Resistant Bacteria ◽  
Bacteriophage Therapy ◽  
Antibiotic Resistant ◽  
Bacterial Diseases ◽  
Wide Range

The lysis of bacteria by bacteriophage was independently discovered by Frederick Twort and Felix d?Herelle but it was d?Herelle who proposed that bacteriophage might be applied to the control of bacterial diseases. Within the former Soviet Union (FSU), bacteriophage therapy was researched and applied extensively for the treatment of a wide range of bacterial infections. In the West, however, it was not explored with the same enthusiasm and was eventually discarded with the arrival of antibiotics. However, the increase in the incidence of multi-antibiotic-resistant bacteria and the absence of effective means for their control has led to increasing international interest in phage therapy and in the long experience of the Eliava Institute. The Eliava Institute of Bacteriophage, Microbiology and Virology (IBMV), which celebrates its 85th anniversary in 2008, was founded in Tbilisi in 1923 through the joint efforts of d?Herelle and the Georgian microbiologist, George Eliava.

Dereplication by Automated Ribotyping of a Competitive Exclusion Culture Bacterial Isolate Library

2006 ◽  
Vol 69 (1) ◽  
pp. 228-232 ◽  
Author(s):  
CYNTHIA SHEFFIELD ◽  
KATE ANDREWS ◽  
ROGER HARVEY ◽  
TAWNI CRIPPEN ◽  
DAVID NISBET
Keyword(s):  
Pathogenic Bacteria ◽  
Competitive Exclusion ◽  
Bacterial Isolate ◽  
Resistant Bacteria ◽  
Restriction Enzyme Digestion ◽  
Bacterial Strains ◽  
Antibiotic Resistant ◽  
Bacterial Diseases ◽  
Disease Management Strategy ◽  
Automated Ribotyping

Concerns over the development of antibiotic-resistant bacteria within the food animal industry have intensified the search for natural approaches to the prevention and treatment of bacterial diseases. Competitive exclusion cultures are the foundation of a disease-management strategy based on the use of benign bacterial strains to prevent the establishment of pathogenic bacteria within a specific host. Differentiation of phenotypically ambiguous isolates is a critical step in establishing a manageable library of bacteria for use in the development of defined competitive exclusion cultures. We used automated ribotyping techniques to dereplicate a large collection of phenotypically ambiguous isolates from a continuous-flow competitive exclusion culture. A total of 157 isolates were screened following an EcoRI restriction enzyme digestion. The 157 isolates were resolved into 23 ribogroups, which represents an 85% reduction in the number of isolates in the bacterial isolate library. Seventy-six percent of the isolates fit into one of five ribogroups. This work demonstrated that automated ribotyping is an effective and efficient tool for dereplication of diverse bacterial isolate libraries.

scholarly journals In Vitro Antimicrobial Screening of Momordica charantia extracts against Multidrug-Resistant Bacterial Strains

2020 ◽  
Vol 2 (2) ◽  
pp. 17-21
Author(s):  
TOUSEEF HUSSAIN ◽  
HINA QAMAR ◽  
SUMBUL REHMAN ◽  
SAIMUL DEHAR
Keyword(s):  
Ethyl Acetate Fraction ◽  
Multidrug Resistant ◽  
Momordica Charantia ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Antibiotic Resistant ◽  
Bacterial Diseases ◽  
Concentration Method ◽  
First Time

A comparative study for screening the antibiotic potential of Momordica charantia successive extracts in vitro against eleven multidrug-resistant bacterial strains. Momordica charantia fresh fruit was extracted successively in different solvents in the order of increasing polarity from hexane to aqueous followed by screening against eleven antibiotic-resistant bacterial strains including both Gram-positive and Gram-negative bacterial strains using Kirby-Bauer’s disk diffusion and agar well diffusion method. A study on Staphylococcus epidermidis and Corynebacterium xerosis are reported for the first time. Significant inhibitory activity was noted against most of the resistant human pathogenic strains. Findings reported that ethyl acetate fraction shows the highest zone of inhibition while hexane, petroleum ether, chloroform and aqueous extract were almost resistant. The antibacterial efficacy of Momordica charantia is found to be significant. It’s also concluded that the controversy on the antibacterial activity of Momordica charantia fruit extract in different solvents is based on several factors like the solvent used for extraction, plant part, concentration, method of extraction, etc. M. charantia extracts could be used as an alternative anti-microbial to replace antibiotics for treating a broad spectrum of multidrug-resistant bacterial diseases.

scholarly journals Development of Effective Antibacterial Treatment: Lessons from the Past and Novel Approaches

Antibiotics ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 230
Author(s):  
Mariagrazia Di Luca ◽  
Tiziano Marzo
Keyword(s):  
Antibacterial Treatment ◽  
Bacterial Strains ◽  
Antibiotic Resistant ◽  
The Past ◽  
Novel Approaches ◽  
Rapid Diffusion

In the last three decades, the appearance and rapid diffusion of antibiotic-resistant bacterial strains have been observed [...]

scholarly journals Assessment of the microbiome during bacteriophage therapy in combination with systemic antibiotics to treat a case of staphylococcal device infection

Microbiome ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Andre Mu ◽  
Daniel McDonald ◽  
Alan K. Jarmusch ◽  
Cameron Martino ◽  
Caitriona Brennan ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Phage Therapy ◽  
Ecological Impacts ◽  
Skin Microbiome ◽  
Bacteriophage Therapy ◽  
Bacterial Diseases ◽  
Device Infection ◽  
Global Health Issue ◽  
Serious Bacterial Infections ◽  
Systemic Antibiotics

Abstract Background Infectious bacterial diseases exhibiting increasing resistance to antibiotics are a serious global health issue. Bacteriophage therapy is an anti-microbial alternative to treat patients with serious bacterial infections. However, the impacts to the host microbiome in response to clinical use of phage therapy are not well understood. Results Our paper demonstrates a largely unchanged microbiota profile during 4 weeks of phage therapy when added to systemic antibiotics in a single patient with Staphylococcus aureus device infection. Metabolomic analyses suggest potential indirect cascading ecological impacts to the host (skin) microbiome. We did not detect genomes of the three phages used to treat the patient in metagenomic samples taken from saliva, stool, and skin; however, phages were detected using endpoint-PCR in patient serum. Conclusion Results from our proof-of-principal study supports the use of bacteriophages as a microbiome-sparing approach to treat bacterial infections.

scholarly journals 1564. Activity of bacteriophage against multidrug resistant Klebsiella pneumoniae

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S782-S782
Author(s):  
Sailaja Puttagunta ◽  
Maya Kahan-Haanum ◽  
Sharon Kredo-Russo ◽  
Eyal Weinstock ◽  
Efrat Khabra ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Unmet Need ◽  
Multidrug Resistant ◽  
Beta Lactamase ◽  
Bacterial Strains ◽  
Antibiotic Resistant ◽  
Extended Spectrum Beta Lactamase ◽  
Novel Approach ◽  
Infection Types

Abstract Background The prevalence of extended-spectrum beta-lactamase (ESBL) producing and carbapenem resistant (CR) Klebsiella pneumoniae (KP) has significantly risen in all geographic regions. Infections due to these bacteria are associated with high mortality across different infection types. Even with newer options, there remains an unmet need for safe and effective therapeutic options to treat infections caused by ESBL and CR KP. Phage therapy offers a novel approach with an unprecedented and orthogonal mechanism of action for treatment of diseases caused by pathogenic bacterial strains that are insufficiently addressed by available antibiotics. Phage-based therapies confer a high strain-level specificity and have a strong intrinsic safety profile. Here we describe the identification of novel phages that can effectively target antibiotic resistant KP strains. Host range of the 21 phages on 33 strain KP panel via solid culture infectivity assays. Red marks resistance to infection while sensitivity to phage is marked in green Methods KP clinical strains were isolated from human stool specimens preserved in glycerol. Selective culturing was carried, followed by testing of individual colonies for motility, indole and urease production, sequenced and analyzed by Kleborate tool to determine antibiotic resistant genes. Natural phages were isolated from plaques that developed on susceptible bacterial targets, sequenced and characterized. Results Antibiotic-resistant KP strains encoding beta lactamase genes or a carbapenemase (n=33) were isolated from healthy individuals (n=3), and patients with inflammatory bowel disease (n=26) or primary sclerosing cholangitis (n=3). Isolates sequencing revealed bla CTX-M15 and/or bla SHV encoding strains and carbapenamase KPC-2. A panel of 21 phages targeting the beta-lactamase- and carbapenemase-producing KP strains were identified. Phage sequencing revealed that all phages belong to the Caudovirales order and include 6 Siphoviridae, 14 Myoviridae, and 1 Podoviridae. In vitro lytic activity of the phages was tested on the isolated bacteria and revealed a coverage of 70% of the 33 isolated antibiotic resistant strains, >50% of which were targeted by multiple phages. Conclusion Collectively, these results demonstrate the feasibility of identifying phage with potent activity against antibiotic resistant KP strains, and may provide a novel therapeutic approach for treatment of ESBL and CR KP infections. Disclosures All Authors: No reported disclosures

scholarly journals Polymeric Systems as Antimicrobial or Antifouling Agents

2019 ◽  
Vol 20 (19) ◽  
pp. 4866 ◽  
Author(s):  
Iolanda Francolini ◽  
Antonella Piozzi
Keyword(s):  
Bacterial Strains ◽  
Antibiotic Resistant ◽  
Polymeric Systems

The rapid increase in the emergence of antibiotic-resistant bacterial strains combined with a dwindling rate of discovery of novel antibiotic molecules has lately created an alarming issue worldwide [...]

Advances on chemically modified antimicrobial peptides for generating peptide antibiotics

2021 ◽  
Author(s):  
Samilla Rezende ◽  
karen k.n. oshiro ◽  
Nelson Junior ◽  
Octavio L. Franco ◽  
Marlon Henrique Cardoso
Keyword(s):  
Clinical Trials ◽  
Antimicrobial Peptides ◽  
Bacterial Infections ◽  
Peptide Antibiotics ◽  
Antibiotic Resistant ◽  
Chemically Modified

Antimicrobial peptides (AMPs) are pinpointed as promising molecules against antibiotic-resistant bacterial infections. Nevertheless, there is a discrepancy between the AMP sequences generated and the tangible outcomes in clinical trials. AMPs’...

scholarly journals Anemia in primary hyperparathyroidism

2020 ◽  
Vol 65 (4) ◽  
pp. 514-526
Author(s):  
A. M. Gorbacheva ◽  
S. S. Shklyayev ◽  
A. K. Eremkina ◽  
A. A. Bratchikova ◽  
N. G. Mokrysheva
Keyword(s):  
Chronic Kidney Disease ◽  
Cell Proliferation ◽  
Clinical Trials ◽  
Kidney Disease ◽  
Blood Loss ◽  
Primary Hyperparathyroidism ◽  
Underlying Disease ◽  
Erythroid Cell ◽  
Specific Factors ◽  
Clinical Description

Introduction. Anaemia is a complication of primary hyperparathyroidism (PHPT). Pathogenesis of PHPT-induced anaemia involves inhibited erythroid cell proliferation associated with the underlying disease and non-specific factors (blood loss, chronic kidney disease). However, its specific mechanisms remain unclear.Aim. Clinical description of a PHPT case with multifactorial complicating anaemia.Main findings. With putative evidence existing on relationships between PHPT and anaemia, no large clinical trials substantiated suitable algorithms for such patients’ management. The genesis of anaemia reported in this case was multifactorial and not decisively excluding PHPT from putative causes.

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