scholarly journals Evaluation of the Interactions between Human Serum Albumin (HSA) and Non-Steroidal Anti-Inflammatory (NSAIDs) Drugs by Multiwavelength Molecular Fluorescence, Structural and Computational Analysis

2021 ◽  
Vol 14 (3) ◽  
pp. 214
Author(s):  
Susana Amézqueta ◽  
José Luís Beltrán ◽  
Anna Maria Bolioli ◽  
Lluís Campos-Vicens ◽  
Francisco Javier Luque ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Structural Information ◽  
Drug Bioavailability ◽  
Key Factor ◽  
Star Program ◽  
Molecular Fluorescence ◽  
Anti Inflammatory ◽  
Complementary Techniques

The interaction between drugs and transport proteins, such as albumins, is a key factor in drug bioavailability. One of the techniques commonly used for the evaluation of the drug-protein complex formation is fluorescence. This work studies the interaction of human serum albumin (HSA) with four non-steroidal anti-inflammatory drugs (NSAIDs)—ibuprofen, flurbiprofen, naproxen, and diflunisal—by monitoring the fluorescence quenching when the drug-albumin complex is formed. Two approaches—the double logarithm Stern-Volmer equation and the STAR program—are used to evaluate the binding parameters. The results are analyzed considering the binding properties, determined by using other complementary techniques and the available structural information of albumin complexes with NSAID-related compounds. Finally, this combined analysis has been synergistically used to interpret the binding of flurbiprofen to HSA.

Anti-Inflammatory Activity in the Low Molecular Weight Fraction of Commercial Human Serum Albumin (LMWF5A)

2015 ◽  
Vol 37 (1) ◽  
pp. 55-67 ◽  
Author(s):  
Gregory W. Thomas ◽  
Leonard T. Rael ◽  
Charles W. Mains ◽  
Denetta Slone ◽  
Matthew M. Carrick ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Weight Fraction ◽  
Inflammatory Activity ◽  
Low Molecular Weight ◽  
Anti Inflammatory

scholarly journals Effect of different parameters on the binding of two anti-inflammatory drugs to human serum albumin

1986 ◽  
Vol 24 (4) ◽  
pp. 1031-1037
Author(s):  
Cristina Zona ◽  
Gianna Roscetti ◽  
Francesca Venturelli ◽  
L. Giorgio Roda
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Inflammatory Drugs ◽  
Anti Inflammatory

scholarly journals Interaction studies of chiral non-steroidal anti-inflammatory drugs with HSA protein using capillary electrophoresis frontal analysis and electrokinetic chromatography

2015 ◽  
Author(s):  
◽  
Sinegugu Khulu
Keyword(s):  
Capillary Electrophoresis ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Binding Constants ◽  
Binding Energies ◽  
Frontal Analysis ◽  
Drug Concentrations ◽  
Inflammatory Drugs ◽  
Anti Inflammatory

Human Serum Albumin (HSA) predominantly found in the blood plasma proteins, acts as a carrier for many drugs. In the present work binding interactions of eight arylpropionate non-steroidal anti-inflammatory drugs (NSAIDs) were studied with Human Serum Albumin HSA using Capillary Electrophoresis (CE) under physiological conditions. The concentration of HSA was kept constant (525 μM) whereas the drug concentrations were varied between 50-300 μM in each case. The Frontal analysis (FA) and Capillary Zone Electrophoresis (CZE) modes of CE were applied together with a mathematical modelling of the experimental results with a view to obtaining pharmacokinetic properties of each drug. The binding order of the drugs to HSA were established with the three methods together with the mathematical approach. Our studies revealed the presence of more than one binding sites for some of the available drugs. Additionally, molecular docking studies were conducted to establish the binding conformations of drugs in the binding pocket of the HSA. A very good correlation between the computed binding energies (docking) and the experimental binding constants were observed throughout this study. The logK values for all eight drugs were ranging from 3.37 - 4.56 for FA, 3.16 – 4.39 for CZE, and 3.48 – 5.30 for computational studies.

Association of Valdecoxib, a Nonsteroidal Anti-Inflammatory Drug, with Human Serum Albumin

2013 ◽  
Vol 89 (6) ◽  
pp. 1399-1405 ◽  
Author(s):  
Maria V. Encinas ◽  
Eduardo Lissi ◽  
Claudio Vergara
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Inflammatory Drug ◽  
Anti Inflammatory

Solution equilibria and the X-ray structure of Cu(ii) complexation with 3-amino-N-(pyridin-2-ylmethyl)propanamide, a pseudo-mimic of human serum albumin

2016 ◽  
Vol 45 (42) ◽  
pp. 17010-17019 ◽  
Author(s):  
Graham E. Jackson ◽  
Fatin M. Elmagbari ◽  
Ahmed N. Hammouda ◽  
Raffaele P. Bonomo
Keyword(s):  
Rheumatoid Arthritis ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Copper Complexes ◽  
Inflammatory Activity ◽  
X Ray ◽  
Solution Equilibria ◽  
Anti Inflammatory

Copper complexes have anti-inflammatory activity in the treatment of inflammation associated with rheumatoid arthritis (RA).

Glycation of human serum albumin by acylglucuronides of nonsteroidal anti-inflammatory drugs of the series of phenylpropionates

Life Sciences ◽  
1999 ◽  
Vol 65 (12) ◽  
pp. PL151-PL156 ◽  
Author(s):  
Hélène Georges ◽  
Isabelle Jarecki ◽  
Patrick Netter ◽  
Jacques Magdalou ◽  
Françoise Lapicque
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Inflammatory Drugs ◽  
Anti Inflammatory

scholarly journals Interactions Between Sirolimus and Anti-Inflammatory Drugs: Competitive Binding for Human Serum Albumin

2016 ◽  
Vol 6 (2) ◽  
pp. 227-233 ◽  
Author(s):  
Arash Khodaei ◽  
Soheila Bolandnazar ◽  
Hadi Valizadeh ◽  
Leila Hasani ◽  
Parvin Zakeri-Milani
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Competitive Binding ◽  
Inflammatory Drugs ◽  
Anti Inflammatory
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