scholarly journals Protective Effect of Cashew Gum (Anacardium occidentale L.) on 5-Fluorouracil-Induced Intestinal Mucositis

2019 ◽  
Vol 12 (2) ◽  
pp. 51 ◽  
Author(s):  
João Antônio Leal de Miranda ◽  
João Erivan Façanha Barreto ◽  
Dainesy Santos Martins ◽  
Paulo Vitor de Souza Pimentel ◽  
Deiziane Viana da Silva Costa ◽  
...  
Keyword(s):  
Interleukin 1 ◽  
Immunohistochemical Analysis ◽  
Saline Group ◽  
Anacardium Occidentale ◽  
Interleukin 1 Beta ◽  
Villus Height ◽  
Cashew Gum ◽  
Inflammatory Agent ◽  
Intestinal Mucositis ◽  
Cox 2

Intestinal mucositis is a common complication associated with 5-fluorouracil (5-FU), a chemotherapeutic agent used for cancer treatment. Cashew gum (CG) has been reported as a potent anti-inflammatory agent. In the present study, we aimed to evaluate the effect of CG extracted from the exudate of Anacardium occidentale L. on experimental intestinal mucositis induced by 5-FU. Swiss mice were randomly divided into seven groups: Saline, 5-FU, CG 30, CG 60, CG 90, Celecoxib (CLX), and CLX + CG 90 groups. The weight of mice was measured daily. After treatment, the animals were euthanized and segments of the small intestine were collected to evaluate histopathological alterations (morphometric analysis), levels of malondialdehyde (MDA), myeloperoxidase (MPO), and glutathione (GSH), and immunohistochemical analysis of interleukin 1 beta (IL-1β) and cyclooxygenase-2 (COX-2). 5-FU induced intense weight loss and reduction in villus height compared to the saline group. CG 90 prevented 5-FU-induced histopathological changes and decreased oxidative stress through decrease of MDA levels and increase of GSH concentration. CG attenuated inflammatory process by decreasing MPO activity, intestinal mastocytosis, and COX-2 expression. Our findings suggest that CG at a concentration of 90 mg/kg reverses the effects of 5-FU-induced intestinal mucositis.

scholarly journals Troxerutin Prevents 5-Fluorouracil Induced Morphological Changes in the Intestinal Mucosa: Role of Cyclooxygenase-2 Pathway

2020 ◽  
Vol 13 (1) ◽  
pp. 10 ◽  
Author(s):  
João Antônio Leal de Miranda ◽  
Conceição da Silva Martins ◽  
Lázaro de Sousa Fideles ◽  
Maria Lucianny Lima Barbosa ◽  
João Erivan Façanha Barreto ◽  
...  
Keyword(s):  
Chemotherapeutic Agent ◽  
Morphological Changes ◽  
Immunohistochemical Analysis ◽  
Cyclooxygenase 2 ◽  
Villus Height ◽  
Inflammatory Agent ◽  
Cell Counts ◽  
Intestinal Mucositis ◽  
Cox 2

Intestinal mucositis is a common complication associated with 5-fluorouracil (5-FU), a chemotherapeutic agent used for cancer treatment. Troxerutin (TRX), a semi-synthetic flavonoid extracted from Dimorphandra gardneriana, has been reported as a potent antioxidant and anti-inflammatory agent. In the present study, we aimed to evaluate the effect of TRX on 5-FU-induced intestinal mucositis. Swiss mice were randomly divided into seven groups: Saline, 5-FU, TRX-50, TRX-100, TRX-150, Celecoxib (CLX), and CLX + TRX-100. The weight of mice was measured daily. After treatment, the animals were euthanized and segments of the small intestine were collected to evaluate histopathological alterations (morphometric analysis), levels of malondialdehyde (MDA), myeloperoxidase (MPO), glutathione (GSH), mast and goblet cell counts, immunohistochemical analysis, and cyclooxygenase-2 (COX-2) activity. Compared to the saline treatment, the 5-FU treatment induced intense weight loss and reduction in villus height. TRX treatment (100 mg/kg) prevented the 5-FU-induced histopathological changes and decreased oxidative stress by decreasing the MDA levels and increasing GSH concentration. TRX attenuated inflammatory process by decreasing MPO activity, intestinal mastocytosis, and COX-2 expression. TRX also reversed the depletion of goblet cells. Our findings suggest that TRX at a concentration of 100 mg/kg had chemopreventive effects on 5-FU-induced intestinal mucositis via COX-2 pathway.

scholarly journals Losartan improves intestinal mucositis induced by 5-fluorouracil in mice

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Maisie Mitchele Barbosa Oliveira ◽  
Aurigena Antunes de Araújo ◽  
Susana Barbosa Ribeiro ◽  
Polyana Crislayne Moreira de Sales Mota ◽  
Vitória Barros Marques ◽  
...  
Keyword(s):  
Intraperitoneal Administration ◽  
Saline Group ◽  
Common Side Effect ◽  
Type I ◽  
Villus Height ◽  
Stress Markers ◽  
Therapeutic Outcomes ◽  
Angiotensin Ii Antagonist ◽  
Intestinal Mucositis ◽  
Dose Reductions

AbstractIntestinal mucositis (IM) is a common side effect of 5-fluorouracil (5-FU)-based chemotherapy, which negatively impacts therapeutic outcomes and delays subsequent cycles of chemotherapy resulting in dose reductions and treatment discontinuation. In search of new pharmacological alternatives that minimize your symptoms, this work set out to study the effect of losartan (LOS), a receptor type I (AT1) angiotensin II antagonist, on intestinal mucositis induced by 5-FU. Intestinal mucositis was induced by a single intraperitoneal administration of 5-FU (450 mg/kg) in Swiss mice. Losartan (5, 25 or 50 mg/kg) or saline was orally administered 30 min before 5-FU and daily for 4 days. On 4th day, the animals were euthanized and segments of small intestine were collected to evaluate histopathological alterations (morphometric analysis), concentration of inflammatory cytokines, oxidative stress markers and genic expression of NF-κB p65, Fn-14 and TWEAK. Weight evaluation and changes in leukogram were also analyzed. 5-FU induced intense weight loss, leukopenia and reduction in villus height compared to saline group. Losartan (50 mg/kg) prevented 5-FU-induced inflammation by decreasing in the analyzed parameters compared to the 5-FU group. Our findings suggest that 50 mg/kg of losartan prevents the effects of 5-FU on intestinal mucosa in mice.

Bifidobacterium infantis has a beneficial effect on 5-fluorouracil-induced intestinal mucositis in rats

2015 ◽  
Vol 6 (1) ◽  
pp. 113-118 ◽  
Author(s):  
K.-T. Yuan ◽  
H.-L. Yu ◽  
W.-D. Feng ◽  
P. Chong ◽  
T. Yang ◽  
...  
Keyword(s):  
Body Weight ◽  
Interleukin 1 ◽  
Nuclear Antigen ◽  
Inflammatory Factors ◽  
High Dose ◽  
Villus Height ◽  
Bifidobacterium Infantis ◽  
Toxic Side Effect ◽  
Necrosis Factor Alpha ◽  
Intestinal Mucositis

Intestinal mucositis is a common toxic side effect in cancer patients receiving high-dose chemotherapy. This study aimed to evaluate the beneficial effects of Bifidobacterium infantis in a rat model of intestinal mucositis induced by 5-fluorouracil (5-FU). Thirty male Sprague-Dawley rats were divided into three groups: control, 5-FU, and 5-FU + B. infantis. A single intraperitoneal injection of 5-FU (150 mg/kg) was used to induce intestinal mucositis. B. infantis (1×109 cfu) was administered for 11 days, starting from 7 days before 5-FU injection. Intestinal mucositis was evaluated based on body weight, villus height, immunohistological expression of proliferating cell nuclear antigen (PCNA) and nuclear factor kappa beta (NF-κB), levels of the pro-inflammatory factors interleukin 1 beta and tumour necrosis factor alpha, and myeloperoxidase (MPO) concentration. The results showed that the 5-FU + B. infantis group demonstrated a higher body weight and villus height, increased expression of PCNA, reduced expression of NF-κB and pro-inflammatory factors, and lower MPO concentration compared to the 5-FU group. These data suggest that probiotic B. infantis is effective in reducing chemotherapy-induced intestinal mucositis in rats.

High-Level Secretory Expression and Purification of Recombinant Human Interleukin 1 Beta in Pichia pastoris

2016 ◽  
Vol 23 (8) ◽  
pp. 763-769 ◽  
Author(s):  
Pengfei Li ◽  
Ganggang Yang ◽  
Xiaofang Geng ◽  
Jinbao Shi ◽  
Bin Li ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Interleukin 1 ◽  
Interleukin 1 Beta ◽  
Expression And Purification ◽  
Secretory Expression ◽  
High Level

scholarly journals Purification and characterization of active human interleukin-1 beta-converting enzyme from THP.1 monocytic cells.

1993 ◽  
Vol 268 (24) ◽  
pp. 18062-18069 ◽  
Author(s):  
D.K. Miller ◽  
J.M. Ayala ◽  
L.A. Egger ◽  
S.M. Raju ◽  
T.T. Yamin ◽  
...  
Keyword(s):  
Interleukin 1 ◽  
Interleukin 1 Beta ◽  
Converting Enzyme ◽  
Purification And Characterization ◽  
Monocytic Cells
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