scholarly journals A Novel Genotype and First Record of Trypanosoma lainsoni in Argentina

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 731
Author(s):  
Anahí G. Díaz ◽  
Paula G. Ragone ◽  
Fanny Rusman ◽  
Noelia Floridia-Yapur ◽  
Rubén M. Barquez ◽  
...  
Keyword(s):  
Host Range ◽  
First Record ◽  
Morphological Characterization ◽  
Pairwise Identity ◽  
Wild Mammals ◽  
Transmission Electron ◽  
Chaco Region ◽  
Veterinary Importance ◽  
First Time

Trypanosomes are a group of parasitic flagellates with medical and veterinary importance. Despite many species having been described in this genus, little is known about many of them. Here, we report a genetic and morphological characterization of trypanosomatids isolated from wild mammals from the Argentine Chaco region. Parasites were morphologically and ultrastructurally characterized by light microscopy and transmission electron microscopy. Additionally, 18s rRNA and gGAPDH genes were sequenced and analyzed using maximum likelihood and Bayesian inference. Morphological characterization showed clear characteristics associated with the Trypanosoma genus. The genetic characterization demonstrates that the studied isolates have identical sequences and a pairwise identity of 99% with Trypanosoma lainsoni, which belongs to the clade of lizards and snakes/rodents and marsupials. To date, this species had only been found in the Amazon region. Our finding represents the second report of T. lainsoni and the first record for the Chaco region. Furthermore, we ultrastructurally described for the first time the species. Finally, the host range of T. lainsoni was expanded (Leopardus geoffroyi, Carenivora, Felidae; and Calomys sp., Rodentia, Cricetidae), showing a wide host range for this species.

scholarly journals First record of Palombitrema triangulum (Suriano, 1981) Suriano, 1997 (Monogenea: Dactylogyridae) from freshwater fishes in Brazil

2015 ◽  
Vol 75 (1) ◽  
pp. 229-233
Author(s):  
VD. Abdallah ◽  
RK. Azevedo ◽  
RJ. Silva
Keyword(s):  
Nasal Cavity ◽  
Body Surface ◽  
First Record ◽  
Freshwater Fishes ◽  
Morphological Characterization ◽  
The Body ◽  
Native Fish ◽  
Morphological Differences ◽  
First Time

This study reports for the first time the monogenean Palombitrema triangulum Suriano (1981) in freshwater fishes from Brazil, highlighting new sites of infection for this helminth and some morphological differences. Monogeneans were collected on the body surface, gills and nasal cavity of two native fish species from Brazil, Cyphocharax modestus (Fernández-Yépez, 1948) and Cyphocharax nagelii (Steindachner, 1881). A brief morphological characterization of this species is presented.

scholarly journals Morphological characterization of hemocytes in Ectemnaspis rorotaense (Floch & Abonnenc) and Ectemnaspis trombetense (Hamada, Py-Daniel & Adler) (Diptera: Simuliidae)

2015 ◽  
Vol 8 (3) ◽  
pp. 209-213
Author(s):  
Juciane Conceição da Silva ◽  
Felipe Arley Costa Pessoa ◽  
Cláudia Maria Ríos-Velásquez ◽  
Helena Rocha Côrrea de Araújo ◽  
Ana Paula Sampaio Feitosa ◽  
...  
Keyword(s):  
Cell Types ◽  
Microscopy Study ◽  
Morphological Characterization ◽  
Electron Microscopy Study ◽  
Black Flies ◽  
Morphological Variations ◽  
Transmission Electron ◽  
Amazonas State ◽  
First Time

Abstract. Hemocytes are insect immune cells which are responsible for processes of phagocytosis, encapsulation, and coagulation. This study aim to characterize the hemocyte cells in two species amazon blackflies: Ectemnaspis rorotaense (Floch & Abonnenc) and Ectemnaspis trombetense (Hamada, Py-Daniel & Adler). Black fly larvae and pupae were collected from streams in Presidente Figueiredo Municipality, Amazonas State, Brazil. Hemolymph of 36 individuals of E. rorotaense (12 larvae, 12 pupae and 12 adults) and 38 of E. trombetense (12 larvae, 12 pupae and 14 adults) were collected and the cells were characterized by light microscopy; 200 adults of each species were used to transmission electron microscopy study. In this work were showed, by the first time, the hemocyte cells of black flies amazon. Four cell types were identified: prohemocytes, granulocytes, oenocytoids, and plasmatocytes. Prohemocytes were the smallest cells and they exhibited a high nuclear-cytoplasmic ratio. Granulocytes possessed large, eccentric nuclei, and they were characterized by the presence of granules that differed in size and shape. Oenocytoids presented poorly developed nucleus with localization in central region. Plasmatocytes showed more morphological variations and large projections in the cytoplasmic membrane. The prohemocytes were the most frequent in E. rorotaense, with nearby 45% of total cells, whereas plasmatocytes and granulocytes, each one with 38%, were the most abundant in E. trombetense. This study showed that prohemocytes, granulocytes, oenocytoids, and plasmatocytes were present in the hemolymph of E. rorotaense and E. trombetense during all stages.Caracterização Morfológica de Hemócitos em Ectemnaspis rorotaense (Floch & Abonnenc) e Ectemnaspis trombetense (Hamada, Py-Daniel & Adler) (Diptera: Simuliidae)Resumo. Os hemócitos são células do sistema imune dos insetos responsáveis pelos processos de fagocitose, encapsulação e coagulação. O objetivo desse trabalho foi caracterizar os hemócitos nos simulídeos amazônicos, Ectemnaspis rorotaense (Floch & Abonnenc)e Ectemnaspis trombetense (Hamada, Py-Daniel & Adler). Larvas e pupas de simulídeos foram coletadas em igarapés no município de Presidente Figueiredo, Amazonas, Brasil. Para caracterização celular através da microscopia óptica, foi coletada a hemolinfa de 36 espécimes (12 larvas, 12 pupas e 12 adultos) de E. rorotaense e 38 espécimes (12 larvas, 12 pupas e 14 adultos) de E. trombetense. Para o estudo com microscopia eletrônica de transmissão, 200 adultos de cada espécie foram utilizados. Neste trabalho foram descritos, pela primeira vez, os hemócitos de simulídeos amazônicos. Foram identificados quatro tipos celulares em larvas, pupas e adultos: prohemócitos, plasmatócitos, granulócitos e oenocitóides. Os prohemócitos, com um núcleo volumoso em relação ao citoplasma, se mostraram as menores células. Os granulócitos foram caracterizados pela presença de grânulos de diferentes tamanhos e formas e um núcleo grande e excêntrico. Os oenocitóides revelaram núcleo pouco desenvolvido, geralmente localizado na região central. Os plasmatócitos apresentaram grandes projeções da membrana citoplasmática e maior variação morfológica. Os prohemócitos foram as células mais frequentes em E. rorotaense com 45% do total das células, enquanto os plasmatócitos e granulócitos, ambas com 38% cada, foram as mais abundantes em E. trombetense. Esse estudo mostrou que prohemócitos, granulócitos, oenocitóides e plasmatócitos são presentes na hemolinfa de E. rorotaense and E. trombetense durante todos os estágios.

scholarly journals Morphological and Ultrastructural Characterization of Hemocytes in an Insect Model, the Hematophagous Dipetalogaster maxima (Hemiptera: Reduviidae)

Insects ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 640
Author(s):  
Natalia R. Moyetta ◽  
Fabián O. Ramos ◽  
Jimena Leyria ◽  
Lilián E. Canavoso ◽  
Leonardo L. Fruttero
Keyword(s):  
Cell Biology ◽  
Cell Types ◽  
Transmission Electron ◽  
Ultrastructural Characterization ◽  
Current Classification ◽  
Contrast Microscopy ◽  
First Time ◽  
Controversial Aspect

Hemocytes, the cells present in the hemolymph of insects and other invertebrates, perform several physiological functions, including innate immunity. The current classification of hemocyte types is based mostly on morphological features; however, divergences have emerged among specialists in triatomines, the insect vectors of Chagas’ disease (Hemiptera: Reduviidae). Here, we have combined technical approaches in order to characterize the hemocytes from fifth instar nymphs of the triatomine Dipetalogaster maxima. Moreover, in this work we describe, for the first time, the ultrastructural features of D. maxima hemocytes. Using phase contrast microscopy of fresh preparations, five hemocyte populations were identified and further characterized by immunofluorescence, flow cytometry and transmission electron microscopy. The plasmatocytes and the granulocytes were the most abundant cell types, although prohemocytes, adipohemocytes and oenocytes were also found. This work sheds light on a controversial aspect of triatomine cell biology and physiology setting the basis for future in-depth studies directed to address hemocyte classification using non-microscopy-based markers.

STRUCTURAL AND MORPHOLOGICAL CHARACTERIZATION OF GOLD NANOPARTICLES BY TEM AND DIGITAL CIS TECHNIQUE

2010 ◽  
Vol 09 (05) ◽  
pp. 399-406 ◽  
Author(s):  
A. A. EL-DALY
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Au Nanoparticles ◽  
Morphological Characterization ◽  
Particle Sizes ◽  
Rigid Surface ◽  
Gold Nanocrystals ◽  
Driving Mechanisms ◽  
Transmission Electron

In this paper, we report a convenient and informative procedure for detecting the morphology and surface structure of individual gold nanocrystals using digital Crystal Image Software (CIS) processing of transmission electron microscopy (TEM) image, which comprises coalescence phenomena of these nanoparticles. The results show that the internal structure of Au nanoparticles has a core of gold atoms arranged as a Marks decahedron, surrounded by additional gold–organic compound layers forming a rigid surface layer, and its outer layer comprises four staple motif bridge molecules that resemble handles, formed an unusual pattern. The obtained results improved our understanding of the basics of the coalescence phenomena such as the driving mechanisms acting at different particle sizes. However, these discrete natures of the nanoparticles will assist in the understanding of principles of nanocore assembly and opens a new window for nanoparticles chemistry.

First record of Pulvinaria urbicola Cockerell (Hemiptera: Coccidae) from India, with a key to the Indian species of Pulvinaria Targioni Tozzetti

Zootaxa ◽  
2017 ◽  
Vol 4236 (3) ◽  
pp. 533 ◽  
Author(s):  
SUNIL JOSHI
Keyword(s):  
Host Range ◽  
First Record ◽  
Indian Species ◽  
Broad Host Range ◽  
Similar Species ◽  
Management Options ◽  
Soft Scale ◽  
Plant Pest ◽  
Plant Families ◽  
First Time

The notoriously destructive and invasive soft scale, Pulvinaria urbicola Cockerell (Hemiptera: Coccidae), is recorded for the first time from India. This scale, with variable morphological appearance and similarities with other known scales of the same genus established in India, is redescribed to facilitate its identification and separation from other similar species. Information on its host range, natural enemies and distribution is provided. Management options in the event of an outbreak are discussed briefly. A key to the species of Pulvinaria Targioni Tozzetti in India is also provided. This new arrival warrants special attention in India as it is a potentially damaging plant pest and has a broad host range across many plant families. 

Structural and Morphological Characterization of Ultrathin Films of an Asymmetric Polydiacetylene

1996 ◽  
Vol 440 ◽  
Author(s):  
H. C. Wang ◽  
D. W. Cheong ◽  
J. Kumar ◽  
C. Sung ◽  
S. K. Tripathy
Keyword(s):  
Second Harmonic ◽  
Morphological Characterization ◽  
Lb Films ◽  
Force Microscopy ◽  
Langmuir Blodgett ◽  
Glass Substrates ◽  
Atomic Force ◽  
Transmission Electron ◽  
Air Water Interface

AbstractA soluble, asymmetrically substituted polydiacetylene, poly(BPOD), has been reported to form stable monolayers at the air-water interface by the Langmuir-Blodgett (LB) technique [2]. Preformed polydiacetylene has been deposited onto hydrophobic substrates as multilayers to form second order nonlinear optical thin films. Second harmonic generation was found to increase with the number of layers. From previous atomic force microscopy (AFM) studies backbone orientation along the dipping direction with an interchain spacing of about 5 A° was indicated [2].The film morphology and preferential molecular orientation of these LB films are further investigated by transmission electron microscopy (TEM). A specifically tailored sample preparation method for the ultrathin LB films was used. Multilayer films were deposited on hydrophobic collodion covered glass substrates for this purpose. Electron diffraction was employed to study the crystalline organization of mono and multilayers of LB films as well as cast films.

scholarly journals Preparation and Characterization of Natural Zeolite Modified with Iron Nanoparticles

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Alvaro Ruíz-Baltazar ◽  
Rodrigo Esparza ◽  
Maykel Gonzalez ◽  
Gerardo Rosas ◽  
Ramiro Pérez
Keyword(s):  
Kinetic Models ◽  
Iron Nanoparticles ◽  
Morphological Characterization ◽  
Adsorption Kinetic ◽  
X Ray Diffraction ◽  
Modified Zeolite ◽  
X Ray ◽  
Transmission Electron ◽  
Zeolite Modification

This study is aimed at investigating the structural and morphological characterization of natural and modified zeolite obtained from the state of Oaxaca (Mexico). Iron nanoparticles were used for the zeolite modification. The iron nanoparticles were loaded on the zeolite surface by homogeneous nucleation. Adsorption kinetic models of pseudo first and second order were surveyed. The characterization of pristine and modified zeolite was performed by Fourier transform infrared (FTIR), transmission electron microscopy (TEM), and X-ray diffraction (XRD). From the results, three main phases were identified: clinoptilolite, mordenite, and feldspar. We could also determine the adsorption capacity of the zeolites by means of adsorption kinetic models.

Sign in / Sign up

Export Citation Format

Share Document